Modified Cyclodextrins Are Chemically Defined Glucan Inducers of Defense Responses in Grapevine Cell Cultures

2006 ◽  
Vol 54 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Roque Bru ◽  
Susana Sellés ◽  
Juan Casado-Vela ◽  
Sarai Belchí-Navarro ◽  
Maria Angeles Pedreño
Keyword(s):  
Cell Cultures ◽  
Defense Responses ◽  
Modified Cyclodextrins

scholarly journals Free and Conjugated Benzoic Acid in Tobacco Plants and Cell Cultures. Induced Accumulation upon Elicitation of Defense Responses and Role as Salicylic Acid Precursors

2001 ◽  
Vol 125 (1) ◽  
pp. 318-328 ◽  
Author(s):  
Julie Chong ◽  
Marie-Agnès Pierrel ◽  
Rossitza Atanassova ◽  
Danièle Werck-Reichhart ◽  
Bernard Fritig ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Benzoic Acid ◽  
Cell Cultures ◽  
Defense Responses ◽  
Tobacco Plants

Genes Expressed in Ascochyta raftiei-Inoculated Chickpea Plants and Elicited Cell Cultures as Detected by Differential cDNA-Hybridization

2000 ◽  
Vol 55 (1-2) ◽  
pp. 44-54 ◽  
Author(s):  
Yuki Ichinose ◽  
Karin Tiemann ◽  
Claudia Schwenger-Erger ◽  
Kazuhiro Toyoda ◽  
Frauke Hein ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Defense Mechanism ◽  
Regulation Of Gene Expression ◽  
Gene Activation ◽  
Defense Responses ◽  
Cdna Clones ◽  
Primary Metabolism ◽  
Plant Defense Responses ◽  
Wide Range ◽  
Cdna Hybridization

Abstract In response to the exogenous application of elicitors and attempted invasion by pathogens, plants exhibit a wide range of defense reactions. To understand the defense mechanism s at the level of gene activation and deactivation, differential screenings were perform ed to isolate cDNA clones which are differentially expressed in pathogen-inoculated resistant chickpea plants and elicitor-treated cell cultures. A plenty of genes were isolated and arranged in 5 groups, namely defense-related pathways, signal transduction pathways, regulation of gene expression, catabolic pathways and primary metabolism . Most of these genes were activated although several genes were also found to be suppressed. We discuss the plausible functions of cDNA products in plant defense responses. The cDNA s provide a variety of tools to investigate m olecular mechanisms of defense responses and clearly reflect the massive genomic and metabolic changes which occur during manifestation of antimicrobial defense.

scholarly journals Defense Response in Slash Pine: Chitosan Treatment Alters the Abundance of Specific mRNAs

1997 ◽  
Vol 10 (1) ◽  
pp. 135-137 ◽  
Author(s):  
Mary E. Mason ◽  
John M. Davis
Keyword(s):  
Plant Defense ◽  
Cell Cultures ◽  
Cinnamic Acid ◽  
Differential Display ◽  
Defense Response ◽  
Sequence Similarity ◽  
Defense Responses ◽  
Slash Pine ◽  
Plant Defense Responses ◽  
Specific Mrnas

We used differential display to identify chitosan responsive cDNAs in slash pine cell cultures. Two clones that showed increased mRNA abundance had sequence similarity to genes with roles in major plant defense responses, clone 18 to cinnamic acid 4-hydroxylase, and clone 30 to chitinase.

scholarly journals Plant Cell Cultures as a Tool to Study Programmed Cell Death

2021 ◽  
Vol 22 (4) ◽  
pp. 2166
Author(s):  
Massimo Malerba ◽  
Raffaella Cerana
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Plant Cell ◽  
Cell Cultures ◽  
Cultured Cells ◽  
Defense Responses ◽  
Plant Cell Cultures ◽  
Aerenchyma Formation ◽  
Biotic Stresses ◽  
Whole Plant

Programmed cell death (PCD) is a genetically controlled suicide process present in all living beings with the scope of eliminating cells unnecessary or detrimental for the proper development of the organism. In plants, PCD plays a pivotal role in many developmental processes such as sex determination, senescence, and aerenchyma formation and is involved in the defense responses against abiotic and biotic stresses. Thus, its study is a main goal for plant scientists. However, since PCD often occurs in a small group of inaccessible cells buried in a bulk of surrounding uninvolved cells, its study in whole plant or complex tissues is very difficult. Due to their uniformity, accessibility, and reproducibility of application of stress conditions, cultured cells appear a useful tool to investigate the different aspects of plant PCD. In this review, we summarize how plant cell cultures can be utilized to clarify the plant PCD process.

scholarly journals Plant Defense Responses in Opium Poppy Cell Cultures Revealed by Liquid Chromatography-Tandem Mass Spectrometry Proteomics

2008 ◽  
Vol 8 (1) ◽  
pp. 86-98 ◽  
Author(s):  
Katherine G. Zulak ◽  
Morgan F. Khan ◽  
Joenel Alcantara ◽  
David C. Schriemer ◽  
Peter J. Facchini
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Plant Defense ◽  
Cell Cultures ◽  
Defense Responses ◽  
Tandem Mass ◽  
Plant Defense Responses ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

Bacteriophages Associated with Turkey Coecums in Bluecomb-Infected and Healthy Birds

1969 ◽  
Vol 27 ◽  
pp. 226-227
Author(s):  
A. E. Ritchie
Keyword(s):  
Host Cell ◽  
Causal Relationship ◽  
Cell Cultures ◽  
Cell Interaction ◽  
Etiologic Agent ◽  
Viral Origin ◽  
Intestinal Contents ◽  
Host Cell Interaction

The cause of bluecomb disease in turkeys is unknown. Filtration of infective intestinal contents suggests a viral origin. To date, it has not been possible to isolate the etiologic agent in various cell cultures. The purpose of this work was to characterize as many virus-like entities as were recognizable in intestines of both healthy and bluecomb-infected turkeys. By a comparison of the viral populations it was hoped that some insight might be gained into the cause of this disease. Studies of turkey hemorraghic enteritis by Gross and Moore (Avian Dis. 11: 296-307, 1967) have suggested that a bacteriophage-host cell interaction may bear some causal relationship to that disease.

The Fine Structure of Rat Granulosa Cell Cultures Correlated with Progestin Secretion

1973 ◽  
Vol 31 ◽  
pp. 552-553
Author(s):  
T. M. Crisp ◽  
F.R. Denys
Keyword(s):  
Fine Structure ◽  
Granulosa Cell ◽  
Cell Cultures ◽  
Single Injection ◽  
Surrounding Medium ◽  
Petri Dish ◽  
Female Rats ◽  
Vaginal Smear ◽  
Immature Female ◽  
Serum Gonadotropin

The purpose of this paper is to present observations on the fine structure of rat granulosa cell cultures grown in the presence of an adenohypophyseal explant and to correlate the morphology of these cells with progestin secretion. Twenty-six day old immature female rats were given a single injection of 5 IU pregnant mares serum gonadotropin (PMS) in order to obtain ovaries with large vesicular follicles. At 66 hrs. post-PMS administration (estrus indicated by vaginal smear cytology), the ovaries were removed and placed in a petri dish containing medium 199 and 100 U penicillin/streptomycin (P/S)/ml. Under a 20X magnification dissecting microscope, some 5-8 vesicular follicles/ovary were punctured and the granulosa cells were expressed into the surrounding medium. The cells were transferred to centrifuge tubes and spun down at 1000 rpm for 5 mins.

Morphological Examination of a Herpes-type Virus Indigenous for Tree Shrews

1970 ◽  
Vol 28 ◽  
pp. 160-161
Author(s):  
J. P. Brunschwig ◽  
R. M. McCombs ◽  
R. Mirkovic ◽  
M. Benyesh-Melnick
Keyword(s):  
Electron Microscopy ◽  
Soft Tissue ◽  
Chick Embryo ◽  
Soft Tissue Sarcoma ◽  
Cell Cultures ◽  
Tree Shrew ◽  
Type Virus ◽  
Morphological Examination ◽  
Tree Shrews ◽  
Embryo Cells

A new virus, established as a member of the herpesvirus group by electron microscopy, was isolated from spontaneously degenerating cell cultures derived from the kidneys and lungs of two normal tree shrews. The virus was found to replicate best in cells derived from the homologous species. The cells used were a tree shrew cell line, T-23, which was derived from a spontaneous soft tissue sarcoma. The virus did not multiply or did so poorly for a limited number of passages in human, monkey, rodent, rabbit or chick embryo cells. In the T-23 cells, the virus behaved as members of the subgroup B of herpesvirus, in that the virus remained primarily cell associated.

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