Effects of Administration of beta-Carotene, Ascorbic Acid, Persimmons, and Pods on Antioxidative Ability in UV-Irradiated ODS Rats

2005 ◽  
Vol 75 (4) ◽  
pp. 227-234 ◽  
Author(s):  
Hosotani ◽  
Yoshida ◽  
Kitagawa
Keyword(s):  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Beta Carotene ◽  
Thiobarbituric Acid ◽  
Ultraviolet B ◽  
Thiobarbituric Acid Reactive Substances ◽  
Sod Activity ◽  
Antioxidative Ability

To evaluate the effects of supplementing diets with carotenoid and ascorbic acid (AsA) on the antioxidative ability of Osteogenic Disorder-Shionogi (ODS) rats, we added synthetic b-carotene (bC), AsA, and powders of persimmon (Ka) and pods (Po) containing bC and AsA to the diet and obtained the following results. The urinary 8-hydroxydeoxyguanosine (8-OHdG) concentration was low in the –betaC•AsA and +AsA groups but high in the +betaC. AsA, +Ka, and +Po groups. The thiobarbituric acid-reactive substances (TBARS) in both the liver and skin were higher in the –betaC•AsA group than in the +betaC•AsA group and were low in the +Ka and +Po groups. As antioxidant enzymes, glutathione peroxidase (GSH-Px) activity was high in the +betaC•AsA group, low in the –betaC•AsA group in both the skin and liver, and also high in the + Ka and +Po group in the liver. Superoxide dismutase (SOD) activity was high in the –betaC•AsA group and low in the +betaC•AsA and +Ka groups in both the skin and liver. Catalase (CAT) activity in the liver was low in the –betaC•AsA, + AsA, and +betaC groups and high in the +betaC•AsA and + Po groups. These results confirmed that the administration of betaC, AsA, and persimmons and pods increases antioxidative ability in the skin and liver of ultraviolet-b(UV-B)-irradiated ODS rats.

Research on the Antioxidation of Torreya Grandis Alcohol Extracts in Cholesterol-Fed Rats

2013 ◽  
Vol 690-693 ◽  
pp. 1366-1369
Author(s):  
Qin Fang ◽  
Sheng Lang Jin
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Thiobarbituric Acid ◽  
Enzymatic Activities ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Sod Activity ◽  
Torreya Grandis

The antioxidant enzymatic activities of Torreya grandis alcohol extracts (TAE) in cholesterol-fed rats and an index of lipid peroxidation had been identified In the TAE groups, compared with the control group, significant decreases were observed in superoxide dismutase (SOD) activity in the livers and kidneys, catalase (CAT) activity in the kidneys, and glutathione peroxidase (GSH-Px) activity in the liver and kidneys. Besides, significant decreases were observed in the quantities of thiobarbituric acid reactive substances (TBARS) in the liver and kidneys of the TAE groups.

scholarly journals Lipid Peroxides and Antioxidant Enzymes in Cisplatin-Induced Chronic Nephrotoxicity in Rats

2005 ◽  
Vol 2005 (3) ◽  
pp. 139-143 ◽  
Author(s):  
Ricardo González ◽  
Cheyla Romay ◽  
Aluet Borrego ◽  
Frank Hernández ◽  
Nelson Merino ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Antioxidant System ◽  
Renal Damage ◽  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Repeated Doses

Cisplatin (CDDP), an anticancer drug, induces remarkable toxicity in the kidneys of animals and humans and it has been well documented that reactive oxygen species and the renal antioxidant system are strongly involved in acute renal damage induced by CDDP. The aim of the present study was to investigate whether or not the renal antioxidant system plays also an important role in chronic renal damage induced by repeated doses of CDDP (1 mg/kg intraperitoneally twice weekly during 10 weeks in rats). In order to elucidate it, serum creatinine and urea levels, renal glutathione and thiobarbituric acid-reactive substances (TBARS) content, as well as renal superoxide dismutase and glutathione peroxidase activities were measured in the kidney homogenates of chronically CDDP-treated rats and additionally histological studies were performed in the rat kidneys. The chronic treatment with CDDP induced a significant increase in creatinine and urea levels in serum, but the other parameters mentioned above were not significantly modified as compared to the values in nontreated rats. Taking into account these results, we conclude that chronic CDDP administration induces also severe nephrotoxicity, in contrast to CDDP acute application, without any significant modification in the activity of relevant antioxidant enzymes such as superoxide dismutase and glutathione peroxidase, renal glutathione and lipid peroxides, by which the role of the antioxidant system in chronic nephrotoxicity induced by CDDP in rats is uncertain.

Olive cake reduces glycaemia and lipemia and increases antioxidant enzymes in STZ-induced diabetes in rat erythrocytes and tissues

2019 ◽  
Vol 50 (2) ◽  
pp. 360-372
Author(s):  
Hayet Cherrad ◽  
Sherazede Bouderbala ◽  
Yahiaoui Zidan ◽  
Djamil Krouf
Keyword(s):  
Antioxidant Enzymes ◽  
Heart Muscle ◽  
Design Methodology ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Thiobarbituric Acid Reactive Substances ◽  
Olive Cake ◽  
Sod Activity ◽  
Content Type ◽  
Antioxidant Enzymes Activities

Purpose The purpose of this study is to determine the effect of olive cake (CO) on glycaemia and lipemia and lipid peroxidation and antioxidant enzymes activities in erythrocytes and tissues, in streptozotocin (STZ)-induced diabetic rats. Design/methodology/approach Diabetes was induced by a single intraperitoneal injection of STZ (55 mg/kg BW). In total, 12 diabetic D rats, weighing 260 ± 20 g, were divided into two groups fed a casein diet supplemented (D-OC) or not (D) with OC (7.5 per cent), for four weeks. Findings In D-OC compared with D, glycaemia, total cholesterol and triglycerides values (−40 per cent; p = 0.007, 27 per cent; p = 0.007 and −27 per cent; p = 0.0019). In erythrocyte, liver, kidney, heart, muscle and brain, thiobarbituric acid reactive substances contents were respectively, (−19 per cent; p = 0.03, −32 per cent; p = 0.002, −20 per cent; p = 0.04, −68 per cent; p = 0.003, −74 per cent; p = 0.0003 and −38 per cent; p = 0.04). In erythrocyte, SOD, GSH-Px and CAT activities were respectively, (+14 per cent; p = 0.01, +74 per cent; p = 0.012 and +34 per cent; p = 0.0009). In the liver, kidney, heart and muscle, SOD activity was respectively, (+31 per cent; p = 0.004, +12 per cent; p = 0.038, +43 per cent; p = 0.001 and +23 per cent; p = 0.18). GSH-Px activity was respectively, (+121 per cent; p = 0.0009, 89 per cent; p = 0.0006, + 95 per cent; p = 0.008, +71 per cent; p = 0.02 and +26 per cent; p = 0.01), in the liver, kidney, heart, muscle and brain. Catalase activity was (+21 per cent; p = 0.008) in the liver, (+88 per cent; p = 0.0002 in the kidney, +53 per cent; p = 0,002 in the heart and 83 per cent; p = 0.00004 in the muscle). Originality/value In diabetic rats, OC reduces hyperglycaemia induced by STZ and attenuates triglyceridemia and cholesterolemia. This residue is able to decrease the oxidative stress by increasing the antioxidant enzymes activity in erythrocytes and tissues. The high contents of phytoconstituents present in OC are considered to be responsible for this effect.

Biological variability of superoxide dismutase, glutathione peroxidase, and catalase in blood

1991 ◽  
Vol 37 (11) ◽  
pp. 1932-1937 ◽  
Author(s):  
L Guemouri ◽  
Y Artur ◽  
B Herbeth ◽  
C Jeandel ◽  
G Cuny ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
The Elderly ◽  
Reference Intervals ◽  
Antioxidant Enzyme Activities ◽  
Biological Variability ◽  
Sod Activity ◽  
Antioxidant Enzymes Activities ◽  
Automated Methods

Abstract We studied the biological variability of blood superoxide dismutase (SOD; EC 1.15.1.1), glutathione peroxidase (GPX; EC 1.11.1.9), and catalase (CAT; EC 1.11.1.6) in a sample of 1836 apparently health subjects, ages 4-97 years. SOD and GPX activities were assayed in plasma (P) and erythrocytes (E) by automated methods, and CAT was measured in erythrocytes by a manual technique. No statistically significant variation of these antioxidant enzyme activities according to gender was demonstrated, except for E-GPX, which was slightly but significantly higher in women than in men (P less than 0.001). Activities appear rather stable in adults less than 65 years old, but decrease for most enzymes in the elderly. There is no evidence that weight, blood pressure, or menopause influences the antioxidant enzymes' activities. In girls ages 10-14 years, E-SOD activity is reduced by 16% (P less than 0.05) after menarche. Variations related to smoking and alcohol consumption are slight and concern only P-SOD and P-GPX, respectively. Conversely, intake of some drugs (e.g., anti-inflammatory agents, antidepressants, and thyroid hormones) modifies activity of some of the three enzymes. E-SOD positively correlates with P-SOD (r = 0.216, P less than 0.001) and E-CAT (r = 0.123, P less than 0.001), and E-GPX with P-GPX (r = 0.218, P less than 0.001). Finally, we propose reference intervals for activities of the three antioxidant enzymes in blood in individuals less than 65 years old.

scholarly journals Liver and serum antioxidant status after methanol intoxication in rats.

1997 ◽  
Vol 44 (1) ◽  
pp. 139-145 ◽  
Author(s):  
E Skrzydlewska ◽  
R Farbiszewski
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Alpha Tocopherol ◽  
Thiobarbituric Acid Reactive Substances ◽  
Methanol Intoxication ◽  
Sh Groups ◽  
Sulfhydryl Compounds ◽  
Antioxidant Mechanisms

Activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and glutathione reductase (GSSG-R) and concentration of ascorbate, alpha-tocopherol, non-protein and protein-bound sulfhydryl compounds and thiobarbituric acid-reactive substances (TBA-rs) were measured in liver and serum of rats 6, 12 and 24 h and 2, 5 and 7 days after intoxication with 1.5 g or 3.0 g methanol/kg b.w. Liver GSH-Px and GSSG-R activities and SH-groups and ascorbate content were significantly diminished at 6 and 24 h, while TBA-rs were increased. Serum SOD, GSH-Px and GSSG-R activities and SH-groups concentration were reduced while TBA-rs were elevated. The changes were more intensive after application of the higher dose of methanol. It is concluded that methanol impairs the liver and blood serum antioxidant mechanisms in rats.

scholarly journals SESAMOL ANTAGONIZES ROTENONE-INDUCED CELL DEATH IN SH-SY5Y NEURONAL CELLS

2016 ◽  
Vol 8 (12) ◽  
pp. 72
Author(s):  
Rohini D. ◽  
Vijayalakshmi K.
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Superoxide Dismutase ◽  
Membrane Potential ◽  
Glutathione Peroxidase ◽  
Cell Viability ◽  
Mitochondrial Membrane ◽  
Thiobarbituric Acid ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances

<p><strong>Objective: </strong>To investigate the neuroprotective effect of sesamol against rotenone-induced cell death in SH-SY5Y cells associated with Parkinsonism.</p><p><strong>Methods: </strong>SH-SY5Y cells were maintained in Dulbecco’s modified Eagle’s medium. After differentiation, the cells were incubated with rotenone (20 μM) and sesamol at different concentrations (10-100 μM). Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. The reactive oxygen species, mitochondrial membrane potential and nuclear morphology were determined by dichlorofluorescein diacetate, rhodamine 123 and 4’, 6-diamidino-2-phenylindole, respectively. Thiobarbituric acid reactive substances, activities of catalase, superoxide dismutase, and glutathione peroxidase and glutathione level were determined by standard assays.</p><p><strong>Results: </strong>Sesamol significantly increased the cell viability and decreased the rotenone-induced cell death in SH-SY5Y cells. Sesamol antagonized rotenone-induced reactive oxygen species generation, loss of mitochondrial membrane potential and nuclear damage. Sesamol also decreased thiobarbituric acid reactive substances level, increased the activities of catalase, superoxide dismutase, glutathione peroxidase and increased the level of glutathione in rotenone-induced cells.</p><p><strong>Conclusion: </strong>The results obtained strongly indicate the promising neuroprotective role of sesamol against rotenone-induced death in SH-SY5Y cells.</p>

scholarly journals Volatile Organic Compounds, Oxidative and Sensory Patterns of Vacuum Aged Foal Meat

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1495 ◽  
Author(s):  
Alessandra Tateo ◽  
Aristide Maggiolino ◽  
Ruben Domínguez ◽  
José Manuel Lorenzo ◽  
Francesca Rita Dinardo ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Volatile Compounds ◽  
Aging Time ◽  
Sensory Evaluation ◽  
Thiobarbituric Acid ◽  
Protein Carbonyls ◽  
Thiobarbituric Acid Reactive Substances ◽  
Volatile Organic ◽  
Independent Variable

The study aimed to evaluate the effect of 14-day vacuum aging on the volatile compounds (VOC) profile, oxidative profile, antioxidant enzymes activity, and sensory evaluation in the Longissimusthoracis muscle of foal meat under vacuum aging. Longissimusthoracis (LT) was sampled in 20 mm thick slices, vacuum packed, and stored at 4 °C. Samples were randomly assigned to different aging times (1, 6, 9, 14 days after slaughtering). VOCs, thiobarbituric acid reactive substances (TBARs), hydroperoxides, carbonyl proteins, superoxide dismutase, catalase, and glutathione peroxidase were analyzed, and a sensory test was performed. A nested one-way analysis of variance (ANOVA) was performed for aging time as an independent variable. Significance was set at p < 0.05. The main VOCs originating from cooked steaks were aldehydes, (from 47.18% to 58.81% of the total volatile compounds), followed by hydrocarbons (from 9.32% and 31.99%). TBARs and hydroperoxides did not show variations due to aging (p > 0.05), instead, protein carbonyls showed higher values at the 14th day (p < 0.01). Catalase, superoxide dismutase, and glutathione peroxidase showed increasing values during aging time (p < 0.01). Vacuum aging slowed down lipid oxidation, and protein oxidation was shown to be present. However, the best vacuum aging duration is in the range of 6–9 days from slaughtering, with an improvement of sensory evaluation.

scholarly journals Oxidation-antioxidant balance in the colon mucosa of rats at different times points after ceftriaxone administration

2017 ◽  
Vol 22 (1) ◽  
pp. 11-15
Author(s):  
Y. Holota ◽  
A. Bazan ◽  
G. Tolstanova
Keyword(s):  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Colon Mucosa ◽  
Thiol Groups ◽  
Thiobarbituric Acid Reactive Substances ◽  
Protein Thiol ◽  
Sod Activity ◽  
Control Value ◽  
Active Substances

Сeftriaxone administration for 14 days (300 mg/kg, i.m.) increased level of thiobarbituric acid reactive substances and decreased the activity of superoxide dismutase and catalase antioxidant enzymes in the colon mucosa of rats immediately after antibiotics injection. On the 29th day of the experiment (in 14 days after ceftriaxone withdrawal) the level of TBA-active substances still 2.5-fold (P <0.05) exceeded the control value and SOD activity remained below control values to the 72nd day of the experiment. These were accompanied by decreased level of protein thiol groups in 15 and 29 days 1.9-fold (P <0.05) and 1.4-fold (P = 0.08), respectively. Thus antibiotics can lead to long-term oxidative disturbance in the colon mucosa of rats.

scholarly journals Serum levels of brain-derived neurotrophic factor and thiobarbituric acid reactive substances in chronically medicated schizophrenic patients: a positive correlation

2008 ◽  
Vol 30 (4) ◽  
pp. 337-340 ◽  
Author(s):  
Clarissa Severino Gama ◽  
Michael Berk ◽  
Ana Cristina Andreazza ◽  
Flávio Kapczinski ◽  
Paulo Belmonte-de-Abreu
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Neurotrophic Factor ◽  
Thiobarbituric Acid ◽  
Serum Levels ◽  
Brain Derived Neurotrophic Factor ◽  
Thiobarbituric Acid Reactive Substances ◽  
Positive Correlation ◽  
Oxidative Markers ◽  
Schizophrenic Patients

OBJECTIVE: The neurotrophins, antioxidant enzymes and oxidative markers have reciprocal interactions. This report verified in chronically stable medicated schizophrenic patients whether there are correlations between the serum levels of superoxide dismutase, a key enzyme in the antioxidant defense, thiobarbituric acid reactive substances, a direct index of lipid peroxidation, and brain-derived neurotrophic factor, the most widely distributed neurotrophin. METHOD: Sixty DSM-IV schizophrenic patients were included (43 males, 17 females). Mean age was 34.7 ± 10.8 years, mean age at first episode was 19.8 ± 7.9 years, and mean illness duration was 14.9 ± 8.5 years. Each subject had a blood sample collected for the determination of serum levels of brain-derived neurotrophic factor, thiobarbituric acid reactive substances and superoxide dismutase. RESULTS: Brain-derived neurotrophic factor levels showed a positive correlation with thiobarbituric acid reactive substances levels (r = 0.333, p = 0.009). Brain-derived neurotrophic factor levels were not correlated with superoxide dismutase levels (r = - 0.181, p = 0.166), and superoxide dismutase levels were not correlated with thiobarbituric acid reactive substances levels (r = 0.141, p = 0.284). CONCLUSIONS: The positive correlation between brain-derived neurotrophic factor and thiobarbituric acid reactive substances suggests the need of further investigation on intracellular interactions of neurotrophins, antioxidant enzymes and oxidative markers. In addition, this opens a venue for investigation on treatments for the prevention of neurotoxicity along the course of schizophrenia.

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