Chlorophyll Content of Gibberellin-Treated Wheat Seedlings

Higher g value stimuli (gravitational acceleration more than 1 g and referred as hyper gravity) caused by centrifugation have been shown to inhibit elongation growth of various plants. In the present study, effects of high g values were studied on wheat seeds with and without water medium at the time of exposure to high g values. Wheat seeds (variety: LOK-1) were washed with 0.5% fungicide and then 4-5 times with distilled water (D/W). Seeds were then soaked in D/W for 24 hrs. Two different experiments were performed. In initial experiment, soaked seeds were taken into the centrifuge tubes filled with 1 ml D/W. Seeds were exposed to hypergravity ranging from 500 g to 2500 g for 10 minutes. In another experiment, soaked seeds were taken into the centrifuge tubes without water or any other medium and then exposed to high g values. After exposure seeds were immediately sowed on 0.8% agar gel. Results obtained showed that exposure to high g values suppressed growth in wheat seedlings when seeds were exposed to high g values with water as a medium. Chlorophyll content also decreased with increase in g. However, no change in growth and chlorophyll content were observed when seeds were exposed without water medium up to g values as high as 2500. Thus, effects of high g value stimuli depend not only on how much centrifugal force is applied to the seeds but also depends upon how much force is experienced by the inner part of the seeds. Present observation shows that effective centrifugal force experienced by the seeds is different when applied with and without medium.

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Effect of Zno Nanoparticles on Chlorophyll Content of Wheat Plants (Triticum Aestivum L.)

International Journal of Engineering and Advanced Technology - Regular Issue ◽

10.35940/ijeat.c6406.409420 ◽

2020 ◽

Vol 9 (4) ◽

pp. 28-30

Keyword(s):

Zinc Oxide ◽

Seed Germination ◽

Plant Growth ◽

Chlorophyll Content ◽

Zno Nanoparticles ◽

Zinc Oxide Nanoparticles ◽

Control Sample ◽

Oxide Nanoparticles ◽

Noticeable Effect ◽

Wheat Seedlings

Nanoparticles have achieved novel applications in biotechnology and agricultural industries. Nanoparticles on plants may cover a new insight to the ecosystems. There is a need for advance study of the possible effects of the nanoparticles on plant growth and development. In the present investigation, zinc oxide nanoparticles have been prepared by the simple chemical route and were authorized by UV-vis spectrophotometer and X-ray diffraction (XRD) analysis alongwith transmission electron microscope (TEM). The consequence of various concentrations of synthesized zinc oxide nanoparticles on wheat seeds (variety: lok-1) was studied by soaking approach and follow its effect on seedling growth of wheat (at 5 days). The seed germination, plant growth & chlorophyll content characteristics were measured by using standard biophysical techniques and studied. Results showed enhancement in germination and growth characteristics in five days grown wheat seedlings for control upto thousand ppm. Above thousand ppm, the considerable drop off was observed in these parameters upto two thousand ppm. Also, the chlorophyll content in the control sample is greater than the samples treated with the various concentrations of zinc oxide nanoparticles. There was a noticeable effect that employing suitable concentration of ZnO nanoparticles could support the seed germination of wheat in contrast to untreated control.

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Identification of new metribuzin-tolerant wheat (Triticum spp.) genotypes

Crop and Pasture Science ◽

10.1071/cp17017 ◽

2017 ◽

Vol 68 (5) ◽

pp. 401 ◽

Cited By ~ 5

Author(s):

Roopali N. Bhoite ◽

Ping Si ◽

Katia T. Stefanova ◽

Kadambot H. M. Siddique ◽

Guijun Yan

Keyword(s):

Chlorophyll Content ◽

Weed Management ◽

Rapid Assessment ◽

Application Rate ◽

Susceptible Genotype ◽

Wheat Seedlings ◽

Leaf Chlorophyll ◽

Content Index ◽

Triticum Spp ◽

Non Destructive

Herbicide-tolerant wheats are preferred for effective weed management. Rapid phenotyping and effective differential dose are vital for the identification of tolerant genotypes among large quantities of genetic resources. A sand-tray system has been developed to enable rapid assessment of metribuzin damage in wheat seedlings. In total, 946 wheat genotypes were evaluated for metribuzin tolerance by using this system under control and metribuzin-treated conditions. SPAD chlorophyll content index (CCI) offered a non-destructive and rapid analysis of leaf chlorophyll content in wheat seedlings. The application rate for 50% reduction in SPAD CCI (I50) was 3.2-fold higher in the current tolerant genotype (Eagle Rock) than the susceptible genotype Spear. A confirmed dose of 800 g a.i. ha–1 could differentiate between metribuzin-tolerant and -susceptible lines. The experimental design with two-directional blocking followed by statistical analysis to model the spatial variation was instrumental in selecting potential tolerant or susceptible genotypes. Metribuzin reduced chlorophyll by 51.4% in treated seedlings. The overall adjusted mean SPAD CCI ranged from 13.5 to 42.7 for control (untreated) plants and from 0.1 to 29.9 for treated plants. Through repeated validation, nine genotypes had higher chlorophyll content after metribuzin treatment and significantly (P < 0.05) outperformed the tolerant Eagle Rock, whereas 18 genotypes had significantly (P < 0.05) higher chlorophyll reduction than the susceptible Spear. The top five tolerant and susceptible genotypes were selected for a genetic study of metribuzin tolerance. Domesticated forms of tetraploid and hexaploid wheats had higher tolerance to metribuzin, which suggests that the level of domestication and higher ploidy level contributes to metribuzin tolerance. The new sources of tolerance will accelerate breeding for metribuzin tolerance.

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The Effect of Exogenous Application of Quercetin Derivative Solutions on the Course of Physiological and Biochemical Processes in Wheat Seedlings

International Journal of Molecular Sciences ◽

10.3390/ijms22136882 ◽

2021 ◽

Vol 22 (13) ◽

pp. 6882

Author(s):

Marta Jańczak-Pieniążek ◽

Dagmara Migut ◽

Tomasz Piechowiak ◽

Jan Buczek ◽

Maciej Balawejder

Keyword(s):

Gas Exchange ◽

Antioxidant Capacity ◽

Chlorophyll Content ◽

Total Antioxidant Capacity ◽

Total Phenolic ◽

Chlorophyll Content And Fluorescence ◽

Wheat Seedlings ◽

Physiological Processes ◽

Total Antioxidant ◽

Physiological And Biochemical

Quercetin, classified as a flavonoid, is a strong antioxidant that plays a significant role in the regulation of physiological processes in plants, which is particularly important in the case of biotic and abiotic stresses. The study investigated the effect of the use of potassium quercetin solutions in various concentrations (0.5%, 1.0%, 3.0% and 5.0%) on the physiological and biochemical properties of wheat seedlings. A pot experiment was carried out in order to determine the most beneficial dose of this flavonoid acting as a bio-stimulant for wheat plants. Spraying with quercetin derivative solutions was performed twice, and physiological measurements (chlorophyll content and fluorescence as well as gas exchange) were carried out on the first and seventh days after each application. The total phenolic compounds content and the total antioxidant capacity were also determined. It was shown that the concentrations of potassium quercetin applied have a stimulating effect on the course of physiological processes. In the case of most of the tested physiological parameters (chlorophyll content and fluorescence and gas exchange) and the total antioxidant capacity, no significant differences were observed in their increase as a result of application with concentrations of 3.0 and 5.0%. Therefore, the beneficial effect of quercetin on the analysed parameters is already observed when spraying with a concentration of 3.0%.

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Effect of Zno Nanoparticles on Chlorophyll Content of Wheat Plants (Triticum Aestivum L.)

International Journal of Innovative Technology and Exploring Engineering - Special Issue ◽

10.35940/ijeat.c6406.049420 ◽

2020 ◽

Vol 9 (4) ◽

pp. 28-30

Keyword(s):

Zinc Oxide ◽

Seed Germination ◽

Plant Growth ◽

Chlorophyll Content ◽

Zno Nanoparticles ◽

Zinc Oxide Nanoparticles ◽

Control Sample ◽

Oxide Nanoparticles ◽

Noticeable Effect ◽

Wheat Seedlings

Nanoparticles have achieved novel applications in biotechnology and agricultural industries. Nanoparticles on plants may cover a new insight to the ecosystems. There is a need for advance study of the possible effects of the nanoparticles on plant growth and development. In the present investigation, zinc oxide nanoparticles have been prepared by the simple chemical route and were authorized by UV-vis spectrophotometer and X-ray diffraction (XRD) analysis alongwith transmission electron microscope (TEM). The consequence of various concentrations of synthesized zinc oxide nanoparticles on wheat seeds (variety: lok-1) was studied by soaking approach and follow its effect on seedling growth of wheat (at 5 days). The seed germination, plant growth & chlorophyll content characteristics were measured by using standard biophysical techniques and studied. Results showed enhancement in germination and growth characteristics in five days grown wheat seedlings for control upto thousand ppm. Above thousand ppm, the considerable drop off was observed in these parameters upto two thousand ppm. Also, the chlorophyll content in the control sample is greater than the samples treated with the various concentrations of zinc oxide nanoparticles. There was a noticeable effect that employing suitable concentration of ZnO nanoparticles could support the seed germination of wheat in contrast to untreated control.

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Comparative growth and physiological responses of tetraploid and hexaploid species of wheat to flooding stress

Acta agriculturae Slovenica ◽

10.14720/aas.2018.111.2.04 ◽

2018 ◽

Vol 111 (2) ◽

pp. 285

Author(s):

Mohammad Sadegh KHOSRAVI ◽

Reza HEIDARI ◽

Rashid JAMEI ◽

Seyed Mousa MOUSAVI KOUHI ◽

Maryam MOUDI

Keyword(s):

Chlorophyll Content ◽

Hexaploid Wheat ◽

Triticum Turgidum ◽

Sugar Content ◽

Soluble Sugar ◽

Tetraploid Wheat ◽

Root Porosity ◽

Wheat Seedlings ◽

Ploidy Levels ◽

Flooding Stress

Present study is aimed to comparatively investigate the response of two ploidy levels of wheat including a tetraploid (Triticum turgidum L.) and a hexaploid (Triticum aestivum L.) wheat to different durations of flooding stress. Wheat seedlings were exposed to flooding stress for 0, 3, 6 and 9 days. Results showed that all flooding treatments significantly decreased the shoot and root length, and chlorophyll content of both species of wheat. The decrease in chlorophyll content of tetraploid wheat was more than that of hexaploid one. In both species, ADH activity of root was significantly increased under flooding stress, where the increase was more in hexaploid wheat. Flooding stress did not significantly affect root and shoot water content, root porosity, and shoot protein content of any wheat species. Tetraploid and hexaploid wheat used different mechanisms for better tolerance of flooding condition, where tetraploid wheat increased the proline content but in hexaploid wheat, an increase in soluble sugar content was observed.

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Comprehensive evaluation of physiological traits under nitrogen stress and participation of linolenic acid in nitrogen-deficiency response in wheat seedlings

BMC Plant Biology ◽

10.1186/s12870-020-02717-5 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Xiaoxiao Liu ◽

Shiwen Wang ◽

Xiping Deng ◽

Zhiyong Zhang ◽

Lina Yin

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Chlorophyll Content ◽

Linolenic Acid ◽

Acid Composition ◽

Nitrogen Deficiency ◽

Plant Production ◽

Wheat Cultivars ◽

Wheat Seedlings ◽

N Deficiency

Abstract Background Nitrogen (N) deficiency is a major constraint for plant production in many areas. Developing the new crop genotypes with high productivity under N deficiency is an important approach to maintain agricultural production. Therefore, understanding how plant response to N deficiency and the mechanism of N-deficiency tolerance are very important for sustainable development of modern crop production. Results In this study, the physiological responses and fatty acid composition were investigated in 24 wheat cultivars under N-deficient stress. Through Pearson’s correlation analysis and principal component analysis, the responses of 24 wheat cultivars were evaluated. The results showed that the plant growth and carbohydrate metabolism were all differently affected by N deficiency in all tested wheat cultivars. The seedlings that had high shoot biomass also maintained high level of chlorophyll content under N deficiency. Moreover, the changes in fatty acid composition, especially the linolenic acid (18:3) and the double bond index (DBI), showed close positive correlations with the shoot dry weight and chlorophyll content alterations in response to N-deficient condition. These results indicated that beside the chlorophyll content, the linolenic acid content and DBI may also contribute to N-deficiency adaptation, thus could be considered as efficient indicators for evaluation of different response in wheat seedlings under N-deficient condition. Conclusions The alteration in fatty acid composition can potentially contribute to N-deficiency tolerance in plants, and the regulation of fatty acid compositions maybe an effective strategy for plants to adapt to N-deficient stress.

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Effects of SA and NO interaction on root growth and chlorophyll content of wheat seedlings under Cd stress

Acta Ecologica Sinica ◽

10.5846/stxb202007131819 ◽

2021 ◽

Vol 41 (23) ◽

Author(s):

黎桂英，王兆丰，张娅，李嘉敏，蒋晓煜，杨颖丽 LI Guiying

Keyword(s):

Root Growth ◽

Chlorophyll Content ◽

Cd Stress ◽

Wheat Seedlings

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