Calcium phosphate cements for bone engineering and their biological properties

Bacterial adhesion to the calcium phosphate surface is a serious problem in surgery. To prevent bacterial infection, the development of calcium-phosphate cements (CPCs) with bactericidal properties is indispensable. The aim of this study was to fabricate antibacterial CPCs and evaluate their biological properties. Silver-containing tricalcium phosphate (Ag-TCP) microspheres consisting of α/β-TCP phases were synthesized by an ultrasonic spray-pyrolysis technique. The powders prepared were mixed with the setting liquid to fabricate the CPCs. The resulting cements consisting of β-TCP and hydroxyapatite had a porous structure and wash-out resistance. Additionally, silver and calcium ions could be released into the culture medium from Ag-TCP cements for a long time accompanied by the dissolution of TCP. These data showed the bioresorbability of the Ag-TCP cement. In vitro antibacterial evaluation demonstrated that both released and immobilized silver suppressed the growth of bacteria and prevented bacterial adhesion to the surface of CPCs. Furthermore, histological evaluation by implantation of Ag-TCP cements into rabbit tibiae exhibited abundant bone apposition on the cement without inflammatory responses. These results showed that Ag-TCP cement has a good antibacterial property and good biocompatibility. The present Ag-TCP cements are promising for bone tissue engineering and may be used as antibacterial biomaterials.

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Physico-chemical–mechanical and in vitro biological properties of calcium phosphate cements with doped amorphous calcium phosphates

Biomaterials ◽

10.1016/j.biomaterials.2006.10.018 ◽

2007 ◽

Vol 28 (6) ◽

pp. 956-965 ◽

Cited By ~ 85

Author(s):

Marion Julien ◽

Ibrahim Khairoun ◽

Racquel Z. LeGeros ◽

Severine Delplace ◽

Paul Pilet ◽

...

Keyword(s):

Calcium Phosphate ◽

Calcium Phosphates ◽

Biological Properties ◽

Calcium Phosphate Cements ◽

Physico Chemical

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Preparation, Physical-Chemical Characterization, and Cytocompatibility of Polymeric Calcium Phosphate Cements

International Journal of Biomaterials ◽

10.1155/2011/467641 ◽

2011 ◽

Vol 2011 ◽

pp. 1-13 ◽

Cited By ~ 10

Author(s):

Rania M. Khashaba ◽

Mervet Moussa ◽

Christopher Koch ◽

Arthur R. Jurgensen ◽

David M. Missimer ◽

...

Keyword(s):

Calcium Phosphate ◽

Setting Time ◽

Chemical Characterization ◽

Biological Properties ◽

X Ray Diffraction ◽

X Ray ◽

Type Iii ◽

Calcium Phosphate Cements ◽

And Control

Aim. Physicochemical mechanical andin vitrobiological properties of novel formulations of polymeric calcium phosphate cements (CPCs) were investigated.Methods. Monocalcium phosphate, calcium oxide, and synthetic hydroxyapatite were combined with either modified polyacrylic acid, light activated polyalkenoic acid, or polymethyl vinyl ether maleic acid to obtain Types I, II, and III CPCs. Setting time, compressive and diametral strength of CPCs was compared with zinc polycarboxylate cement (control). Specimens were characterized using X-ray diffraction, scanning electron microscopy, and infrared spectroscopy.In vitrocytotoxicity of CPCs and control was assessed.Results. X-ray diffraction analysis showed hydroxyapatite, monetite, and brushite. Acid-base reaction was confirmed by the appearance of stretching peaks in IR spectra of set cements. SEM revealed rod-like crystals and platy crystals. Setting time of cements was 5–12 min. Type III showed significantly higher strength values compared to control. Type III yielded high biocompatibility.Conclusions. Type III CPCs show promise for dental applications.

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Novel magnetic nanocomposite injectables: calcium phosphate cements impregnated with ultrafine magnetic nanoparticles for bone regeneration

RSC Advances ◽

10.1039/c4ra12640h ◽

2015 ◽

Vol 5 (18) ◽

pp. 13411-13419 ◽

Cited By ~ 31

Author(s):

Roman A. Perez ◽

Kapil D. Patel ◽

Hae-Won Kim

Keyword(s):

Magnetic Nanoparticles ◽

Calcium Phosphate ◽

Bone Regeneration ◽

Magnetite Nanoparticles ◽

Biological Properties ◽

Magnetic Nanocomposite ◽

Calcium Phosphate Cements ◽

Physico Chemical

Novel magnetic nanocomposite injectables made of calcium phosphate cements and magnetite nanoparticles demonstrated excellent physico-chemical and biological properties effective for bone regeneration.

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Factors influencing the drug release from calcium phosphate cements

Bioactive Materials ◽

10.1016/j.bioactmat.2021.05.032 ◽

2021 ◽

Author(s):

Marco Fosca ◽

Julietta V. Rau ◽

Vuk Uskoković

Keyword(s):

Calcium Phosphate ◽

Drug Release ◽

Calcium Phosphate Cements ◽

Factors Influencing

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The Potential of Calcium/Phosphate Containing MAO Implanted in Bone Tissue Regeneration and Biological Characteristics

International Journal of Molecular Sciences ◽

10.3390/ijms22094706 ◽

2021 ◽

Vol 22 (9) ◽

pp. 4706

Author(s):

Shun-Yi Jian ◽

Salim Levent Aktug ◽

Hsuan-Ti Huang ◽

Cheng-Jung Ho ◽

Sung-Yen Lin ◽

...

Keyword(s):

Corrosion Resistance ◽

Calcium Phosphate ◽

Soft Tissues ◽

Salt Spray ◽

Surface Characteristics ◽

Corrosion Current ◽

Biological Properties ◽

Bone Tissue Regeneration ◽

Additional Surgery

Micro arc oxidation (MAO) is a prominent surface treatment to form bioceramic coating layers with beneficial physical, chemical, and biological properties on the metal substrates for biomaterial applications. In this study, MAO treatment has been performed to modify the surface characteristics of AZ31 Mg alloy to enhance the biocompatibility and corrosion resistance for implant applications by using an electrolytic mixture of Ca3(PO4)2 and C10H16N2O8 (EDTA) in the solutions. For this purpose, the calcium phosphate (Ca-P) containing thin film was successfully fabricated on the surface of the implant material. After in-vivo implantation into the rabbit bone for four weeks, the apparent growth of soft tissues and bone healing effects have been documented. The morphology, microstructure, chemical composition, and phase structures of the coating were identified by SEM, XPS, and XRD. The corrosion resistance of the coating was analyzed by polarization and salt spray test. The coatings consist of Ca-P compounds continuously have proliferation activity and show better corrosion resistance and lower roughness in comparison to mere MAO coated AZ31. The corrosion current density decreased to approximately 2.81 × 10−7 A/cm2 and roughness was reduced to 0.622 μm. Thus, based on the results, it was anticipated that the development of degradable materials and implants would be feasible using this method. This study aims to fabricate MAO coatings for orthopedic magnesium implants that can enhance bioactivity, biocompatibility, and prevent additional surgery and implant-related infections to be used in clinical applications.

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Mechanical and biological properties of electrodeposited calcium phosphate coatings

Materials Science and Engineering C ◽

10.1016/j.msec.2019.03.020 ◽

2019 ◽

Vol 100 ◽

pp. 475-484 ◽

Cited By ~ 19

Author(s):

T. Mokabber ◽

Q. Zhou ◽

A.I. Vakis ◽

P. van Rijn ◽

Y.T. Pei

Keyword(s):

Calcium Phosphate ◽

Biological Properties ◽

Phosphate Coatings ◽

Calcium Phosphate Coatings

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Therapy and regeneration-combined bone cements for minimally invasive treatment of neoplastic bone defects

Journal of Materials Chemistry B ◽

10.1039/d1tb00703c ◽

2021 ◽

Author(s):

Yu Qu ◽

Hui Zhuang ◽

Meng Zhang ◽

Yufeng Wang ◽

Dong Zhai ◽

...

Keyword(s):

Calcium Phosphate ◽

Minimally Invasive ◽

Tumor Cells ◽

Bone Tumor ◽

Tumor Resection ◽

Bone Defects ◽

Bone Cements ◽

Invasive Treatment ◽

Calcium Phosphate Cements ◽

Bone Tumor Resection

Although calcium phosphate cements (CPC) have been clinically used to repair bone defects caused by bone tumor resection, traditional CPC cannot kill the remaining tumor cells after surgery and prevent...

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Amorphous–Crystalline Calcium Phosphate Coating Promotes In Vitro Growth of Tumor-Derived Jurkat T Cells Activated by Anti-CD2/CD3/CD28 Antibodies

Materials ◽

10.3390/ma14133693 ◽

2021 ◽

Vol 14 (13) ◽

pp. 3693

Author(s):

Yurii P. Sharkeev ◽

Ekaterina G. Komarova ◽

Valentina V. Chebodaeva ◽

Mariya B. Sedelnikova ◽

Aleksandr M. Zakharenko ◽

...

Keyword(s):

T Cells ◽

Calcium Phosphate ◽

Biological Effects ◽

Electrical Potential ◽

Biological Properties ◽

Modern Trend ◽

Calcium Phosphate Coating ◽

Jurkat T Cells ◽

Micro Arc Oxidation

A modern trend in traumatology, orthopedics, and implantology is the development of materials and coatings with an amorphous–crystalline structure that exhibits excellent biocopatibility. The structure and physico–chemical and biological properties of calcium phosphate (CaP) coatings deposited on Ti plates using the micro-arc oxidation (MAO) method under different voltages (200, 250, and 300 V) were studied. Amorphous, nanocrystalline, and microcrystalline statesof CaHPO4 and β-Ca2P2O7were observed in the coatings using TEM and XRD. The increase in MAO voltage resulted in augmentation of the surface roughness Ra from 2.5 to 6.5 µm, mass from 10 to 25 mg, thickness from 50 to 105 µm, and Ca/P ratio from 0.3 to 0.6. The electrical potential (EP) of the CaP coatings changed from −456 to −535 mV, while the zeta potential (ZP) decreased from −53 to −40 mV following an increase in the values of the MAO voltage. Numerous correlations of physical and chemical indices of CaP coatings were estimated. A decrease in the ZP magnitudes of CaP coatings deposited at 200–250 V was strongly associated with elevated hTERT expression in tumor-derived Jurkat T cells preliminarily activated with anti-CD2/CD3/CD28 antibodies and then contacted in vitro with CaP-coated samples for 14 days. In turn, in vitro survival of CD4+ subsets was enhanced, with proinflammatory cytokine secretion of activated Jurkat T cells. Thus, the applied MAO voltage allowed the regulation of the physicochemical properties of amorphous–crystalline CaP-coatings on Ti substrates to a certain extent. This method may be used as a technological mechanism to trigger the behavior of cells through contact with micro-arc CaP coatings. The possible role of negative ZP and Ca2+ as effectors of the biological effects of amorphous–crystalline CaP coatings is discussed. Micro-arc CaP coatings should be carefully tested to determine their suitability for use in patients with chronic lymphoid malignancies.

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Early-stage macroporosity enhancement in calcium phosphate cements by inclusion of poly(N-vinylpyrrolidone) particles as a porogen

Materials Today Communications ◽

10.1016/j.mtcomm.2020.100901 ◽

2020 ◽

Vol 23 ◽

pp. 100901 ◽

Cited By ~ 3

Author(s):

Irene Lodoso-Torrecilla ◽

Floris Stumpel ◽

John A. Jansen ◽

Jeroen J.J.P. van den Beucken

Keyword(s):

Calcium Phosphate ◽

Early Stage ◽

Calcium Phosphate Cements

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