Background:
Diabetes Mellitus (DM) is a major public metabolic disease that influences
366 million people in the world in 2011, and this number is predicted to rise to 552 million in 2030.
DM is clinically diagnosed by a fasting blood glucose that is equal or greater than 7 mM. Therefore,
the development of effective glucose biosensor has attracted extensive attention worldwide. Fluorescence-
based strategies have sparked tremendous interest due to their rapid response, facile operation,
and excellent sensitivity. Many fluorescent compounds have been employed for precise analysis of
glucose, including quantum dots, noble metal nanoclusters, up-converting nanoparticles, organic
dyes, and composite fluorescent microspheres. Silicon dot as promising quantum dots materials have
received extensive attention, owing to their distinct advantages such as biocompatibility, low toxicity
and high photostability.
Methods:
MnO2 nanosheets on the Si nanoparticles (NPs) surface serve as a quencher. Si NPs fluorescence
can make a recovery by the addition of H2O2, which can reduce MnO2 to Mn2+, and the glucose
can thus be monitored based on the enzymatic conversion of glucose by glucose oxidase to generate
H2O2. Therefore, the glucose concentration can be derived by recording the fluorescence recovery
spectra of the Si NPs.
Results:
This probe enabled selective detection of glucose with a linear range of 1-100 μg/mL and a
limit of detection of 0.98 μg/mL. Compared with the commercial glucometer, this method showed
favorable results and convincing reliability.
Conclusion:
We have developed a novel method based on MnO2 -nanosheet-modified Si NPs for
rapid monitoring of blood glucose levels. By combining the highly sensitive H2O2/MnO2 reaction
with the excellent photostability of Si NPs, a highly sensitive, selective, and cost-efficient sensing
approach for glucose detection has been designed and applied to monitor glucose levels in human serum
with satisfactory results.
Advances in superresolution optical fluctuation imaging (SOFI)
