Gynura divaricata exerts hypoglycemic effects by regulating the PI3K/AKT signaling pathway and fatty acid metabolism signaling pathway

Abstract Introduction: Dysregulation of fatty acid metabolism often occurs in tumor, which mainly constitutes of fatty acid synthesis and oxidation. In recent years, studies found that fatty acid metabolism participated in regulation of tumor immune microenvironment, which further influenced the progress of cancer. Thus, it is important to explore the key fatty acid metabolism-related molecules, which not only affects the prognosis of ovarian cancer, but also shows a close correlation with immune microenvironment of cancer.Methods: Database from TCGA was used to explore the fatty acid metabolism-related molecules, which correlated with the prognosis of ovarian cancer using univariate and multivariate cox proportional regression model. Nomogram was constructed to predict the prognostic probability based on ACSM3 and clinicopathological parameters. GDSC database was used to investigate the chemosensitivity of ovarian cancer cells. The correlation between ACSM3 and immune status of ovarian cancer was analyzed by TIMER and TISIDB online tools. In addition, CCK8 assay was used to investigate the chemosensitivity of ovarian cancer cells, real time-PCR and western blot were used to investigate the expression of chemoresistance-related genes.Results: ACSM3 worked as an independent favorable prognostic molecule through univariate and multivariate cox regression analysis. For the use in clinical, nomogram was constructed, and higher expression of ACSM3 showed better prognosis. We found that ACSM3 could regulate PI3K/AKT signaling, and GDSC database showed that PI3K/AKT inhibitor could promote the chemosensitivity of ovarian cancer cells. In addition, the expression of ACSM3 showed significantly correlated with the immune status of ovarian cancer. In vitro experiments showed that ACSM3 can promote the chemosensitivity of ovarian cancer cells by inhibiting PI3K/AKT signaling pathway.Conclusion: Our results showed that ACSM3 acted as a favorable prognostic-related biomarker for ovarian cancer, which could promote chemosensitivity of ovarian cancer through inhibiting PI3K/AKT signaling pathway. This might be due to participate in regulating immune status of ovarian cancer microenvironment.

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Inhibition of fatty acid synthase suppresses U-2 OS cell invasion and migration via downregulating the activity of HER2/PI3K/AKT signaling pathway in vitro

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2013.09.024 ◽

2013 ◽

Vol 440 (2) ◽

pp. 229-234 ◽

Cited By ~ 19

Author(s):

Tao Fang Wang ◽

Heng Wang ◽

Ai Fen Peng ◽

Qing Feng Luo ◽

Zhi Li Liu ◽

...

Keyword(s):

Fatty Acid ◽

Signaling Pathway ◽

Cell Invasion ◽

Fatty Acid Synthase ◽

Akt Signaling ◽

Akt Signaling Pathway ◽

Invasion And Migration ◽

And Migration

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PdpaMn inhibits fatty acid synthase-mediated glycolysis by down-regulating PI3K/Akt signaling pathway in breast cancer

Anti-Cancer Drugs ◽

10.1097/cad.0000000000000968 ◽

2020 ◽

Vol 31 (10) ◽

pp. 1046-1056 ◽

Cited By ~ 1

Author(s):

Eric Achiborebador Okrah ◽

Qiang Wang ◽

Hexiu Fu ◽

Qiuyun Chen ◽

Jing Gao

Keyword(s):

Breast Cancer ◽

Fatty Acid ◽

Signaling Pathway ◽

Fatty Acid Synthase ◽

Akt Signaling ◽

Akt Signaling Pathway

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Involvement of nitric oxide/cyclic GMP signaling pathway in the regulation of fatty acid metabolism in rat hepatocytes

Biochemical Pharmacology ◽

10.1016/s0006-2952(02)01623-4 ◽

2003 ◽

Vol 65 (5) ◽

pp. 807-812 ◽

Cited By ~ 38

Author(s):

Javier Garcı́a-Villafranca ◽

Alberto Guillén ◽

José Castro

Keyword(s):

Nitric Oxide ◽

Fatty Acid ◽

Signaling Pathway ◽

Fatty Acid Metabolism ◽

Cyclic Gmp ◽

Acid Metabolism ◽

Rat Hepatocytes

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Anti-Cancer Properties of Coix Seed Oil against HT-29 Colon Cells through Regulation of the PI3K/AKT Signaling Pathway

Foods ◽

10.3390/foods10112833 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2833

Author(s):

Chunlei Ni ◽

Bailiang Li ◽

Yangyue Ding ◽

Yue Wu ◽

Qiuye Wang ◽

...

Keyword(s):

Fatty Acid ◽

Signaling Pathway ◽

Cell Apoptosis ◽

Seed Oil ◽

Akt Signaling ◽

Regulation Mechanism ◽

Akt Signaling Pathway ◽

Colon Cells ◽

Coix Seed ◽

Ht 29

This study aims to observe the effects of coix seed oil (CSO) on HT-29 cells and investigate its possible regulation mechanism of the PI3K/Akt signaling pathway. Fatty acid analysis showed that coix seed oil mainly contains oleic acid (50.54%), linoleic acid (33.76%), palmitic acid (11.74%), and stearic acid (2.45%). Fourier transform infrared results found that the fatty acid functional groups present in the oil matched well with the vegetable oil band. The results from CCK-8 assays showed that CSO dose-dependently and time-dependently inhibited the viability of HT-29 cells in vitro. CSO inhibited cell viability, with IC50 values of 5.30 mg/mL for HT-29 obtained after 24 h treatment. Morphological changes were observed by apoptotic body/cell nucleus DNA (Hoechst 33258) staining using inverted and fluorescence microscopy. Moreover, flow cytometry analysis was used to evaluate the cell cycle and cell apoptosis. It showed that CSO induced cell apoptosis and cycle arrest in the G2 phase. Quantitative real-time PCR and Western blotting revealed that CSO induced cell apoptosis by downregulating the PI3K/AKT signaling pathway. Additionally, CSO can cause apoptosis in cancer cells by activating caspase-3, up-regulating Bax, and down-regulating Bcl-2. In conclusion, the results revealed that CSO induced G2 arrest and apoptosis of HT-29 cells by regulating the PI3K/AKT signaling pathway.

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Cyanidin-3-O-β-glucoside regulates fatty acid metabolism via an AMP-activated protein kinase-dependent signaling pathway in human HepG2 cells

Lipids in Health and Disease ◽

10.1186/1476-511x-11-10 ◽

2012 ◽

Vol 11 (1) ◽

pp. 10 ◽

Cited By ~ 53

Author(s):

Honghui Guo ◽

Guoling Liu ◽

Ruimin Zhong ◽

Yun Wang ◽

Duan Wang ◽

...

Keyword(s):

Fatty Acid ◽

Protein Kinase ◽

Signaling Pathway ◽

Fatty Acid Metabolism ◽

Hepg2 Cells ◽

Acid Metabolism ◽

Amp Activated Protein Kinase ◽

Dependent Signaling Pathway

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Fatty Acid Synthase Regulates Proliferation and Migration of Colorectal Cancer Cells Via HER2-PI3K/Akt Signaling Pathway

Nutrition and Cancer ◽

10.1080/01635581.2012.701704 ◽

2012 ◽

Vol 64 (6) ◽

pp. 864-870 ◽

Cited By ~ 22

Author(s):

Nan Li ◽

Xiaodong Bu ◽

Xiaoqiang Tian ◽

Peng Wu ◽

Lin Yang ◽

...

Keyword(s):

Colorectal Cancer ◽

Fatty Acid ◽

Cancer Cells ◽

Signaling Pathway ◽

Fatty Acid Synthase ◽

Akt Signaling ◽

Akt Signaling Pathway ◽

Colorectal Cancer Cells ◽

And Migration ◽

Proliferation And Migration

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Network-based approach to identify biomarkers predicting response and prognosis for HER2-negative breast cancer treatment with taxane-anthracycline neoadjuvant chemotherapy

PeerJ ◽

10.7717/peerj.7515 ◽

2019 ◽

Vol 7 ◽

pp. e7515 ◽

Cited By ~ 2

Author(s):

Cui Jiang ◽

Shuo Wu ◽

Lei Jiang ◽

Zhichao Gao ◽

Xiaorui Li ◽

...

Keyword(s):

Breast Cancer ◽

Fatty Acid ◽

Neoadjuvant Chemotherapy ◽

Cancer Treatment ◽

Signaling Pathway ◽

Fatty Acid Metabolism ◽

Acid Metabolism ◽

Breast Cancer Treatment ◽

Chemotherapeutic Response ◽

Ppar Signaling

Objective This study aims to identify effective gene networks and biomarkers to predict response and prognosis for HER2-negative breast cancer patients who received sequential taxane-anthracycline neoadjuvant chemotherapy. Materials and Methods Transcriptome data of training dataset including 310 HER2-negative breast cancer who received taxane-anthracycline treatment and an independent validation set with 198 samples were analyzed by weighted gene co-expression network analysis (WGCNA) approach in R language. Gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis were performed for the selected genes. Module-clinical trait relationships were analyzed to explore the genes and pathways that associated with clinicopathological parameters. Log-rank tests and COX regression were used to identify the prognosis-related genes. Results We found a significant correlation of an expression module with distant relapse–free survival (HR = 0.213, 95% CI [0.131–0.347], P = 4.80E−9). This blue module contained genes enriched in biological process of hormone levels regulation, reproductive system, response to estradiol, cell growth and mammary gland development as well as pathways including estrogen, apelin, cAMP, the PPAR signaling pathway and fatty acid metabolism. From this module, we further screened and validated six hub genes (CA12, FOXA1, MLPH, XBP1, GATA3 and MAGED2), the expression of which were significantly associated with both better chemotherapeutic response and favorable survival for BC patients. Conclusion We used WGCNA approach to reveal a gene network that regulate HER2-negative breast cancer treatment with taxane-anthracycline neoadjuvant chemotherapy, which enriched in pathways of estrogen signaling, apelin signaling, cAMP signaling, the PPAR signaling pathway and fatty acid metabolism. In addition, genes of CA12, FOXA1, MLPH, XBP1, GATA3 and MAGED2 might serve as novel biomarkers predicting chemotherapeutic response and prognosis for HER2-negative breast cancer.

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