scholarly journals Metabolomics-derived marker metabolites to characterize Phaeocystis pouchetii physiology in natural plankton communities

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Constanze Kuhlisch ◽  
Julia Althammer ◽  
Andrey F. Sazhin ◽  
Hans H. Jakobsen ◽  
Jens C. Nejstgaard ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Stationary Phase ◽  
Free Fatty Acids ◽  
Trophic Levels ◽  
The Arctic ◽  
Zooplankton Grazing ◽  
Growth Phases ◽  
Phytoplankton Blooms ◽  
Phaeocystis Pouchetii ◽  
Plankton Communities

AbstractPhaeocystis pouchetii (Hariot) Lagerheim, 1893 regularly dominates phytoplankton blooms in higher latitudes spanning from the English Channel to the Arctic. Through zooplankton grazing and microbial activity, it is considered to be a key resource for the entire marine food web, but the actual relevance of biomass transfer to higher trophic levels is still under discussion. Cell physiology and algal nutritional state are suggested to be major factors controlling the observed variability in zooplankton grazing. However, no data have so far yielded insights into the metabolic state of Phaeocystis populations that would allow testing this hypothesis. Therefore, endometabolic markers of different growth phases were determined in laboratory batch cultures using comparative metabolomics and quantified in different phytoplankton blooms in the field. Metabolites, produced during exponential, early and late stationary growth of P. pouchetii, were profiled using gas chromatography-mass spectrometry. Then, metabolites were characterized that correlate with the growth phases using multivariate statistical analysis. Free amino acids characterized the exponential growth, whereas the early stationary phase was correlated with sugar alcohols, mono- and disaccharides. In the late stationary phase, free fatty acids, sterols and terpenes increased. These marker metabolites were then traced in Phaeocystis blooms during a cruise in the Barents Sea and North Norwegian fjords. About 50 endometabolites of P. pouchetii were detected in natural phytoplankton communities. Mannitol, scyllo-inositol, 24-methylcholesta-5,22-dien-3β-ol, and several free fatty acids were characteristic for Phaeocystis-dominated blooms but showed variability between them. Distinct metabolic profiles were detected in the nutrient-depleted community in the inner Porsangerfjord (< 0.5 µM NO3−, < 0.1 µM PO 4 3− ), with high relative amounts of free mono- and disaccharides indicative for a limited culture. This study thereby shows how the variable physiology of phytoplankton can alter the metabolic landscape of entire plankton communities.

scholarly journals FadD Is Required for Utilization of Endogenous Fatty Acids Released from Membrane Lipids

2011 ◽  
Vol 193 (22) ◽  
pp. 6295-6304 ◽  
Author(s):  
Ángel Pech-Canul ◽  
Joaquina Nogales ◽  
Alfonso Miranda-Molina ◽  
Laura Álvarez ◽  
Otto Geiger ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Stationary Phase ◽  
Free Fatty Acids ◽  
Sinorhizobium Meliloti ◽  
Membrane Lipids ◽  
Membrane Phospholipids ◽  
Content Type ◽  
Fatty Acid Transporter ◽  
In The Wild

FadD is an acyl coenzyme A (CoA) synthetase responsible for the activation of exogenous long-chain fatty acids (LCFA) into acyl-CoAs. Mutation offadDin the symbiotic nitrogen-fixing bacteriumSinorhizobium melilotipromotes swarming motility and leads to defects in nodulation of alfalfa plants. In this study, we found thatS. melilotifadDmutants accumulated a mixture of free fatty acids during the stationary phase of growth. The composition of the free fatty acid pool and the results obtained after specific labeling of esterified fatty acids with a Δ5-desaturase (Δ5-Des) were in agreement with membrane phospholipids being the origin of the released fatty acids.Escherichia colifadDmutants also accumulated free fatty acids released from membrane lipids in the stationary phase. This phenomenon did not occur in a mutant ofE. coliwith a deficient FadL fatty acid transporter, suggesting that the accumulation of fatty acids infadDmutants occurs inside the cell. Our results indicate that, besides the activation of exogenous LCFA, in bacteria FadD plays a major role in the activation of endogenous fatty acids released from membrane lipids. Furthermore, expression analysis performed withS. melilotirevealed that a functional FadD is required for the upregulation of genes involved in fatty acid degradation and suggested that in the wild-type strain, the fatty acids released from membrane lipids are degraded by β-oxidation in the stationary phase of growth.

scholarly journals Lipid and Fatty Acids Accumulation Features of Entomoneis cf. paludosa during Exponential and Stationary Growth Phases in Laboratory Culture

Diversity ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 459
Author(s):  
Yekaterina Bedoshvili ◽  
Yulia Podunay ◽  
Alyona Nikonova ◽  
Artyom Marchenkov ◽  
Elvira Bairamova ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Stationary Phase ◽  
Growth Phase ◽  
Exponential Growth ◽  
Lipid Bodies ◽  
Growth Phases ◽  
Stationary Growth ◽  
Culture Growth

Diatoms are capable of accumulating substantial amounts of triacylglycerides in their cells, which differ in the composition of fatty acids depending on the conditions of cultivation, making them attractive subjects in biotechnology. In the present study, we characterized the structural features of lipid bodies in the diatom Entomoneis cf. paludosa (W. Smith) Reimer strain 8.0727-B and revealed the peculiarities of fatty acid composition in cultures during the stationary and exponential growth phases. Laser scanning confocal microscopy revealed an increased number of lipid bodies in the cytoplasm during the stationary phase of culture growth. Electron microscopy of ultrathin sections showed that an extreme increase in the number and size of plastoglobules in the cells occurs in the stationary phase of culture growth. The gas chromatography with mass spectrometric detection method revealed differences in the fatty acid composition depending on the growth phase. The studied strain can be recommended as a source of hexadecanoic and octadecanoic fatty acids from the culture during the stationary growth phase, as well as eicosapentaenoic fatty acid from the culture during the exponential growth phase.

The Lipid Composition of the Coccolithophore Emiliania Huxleyi and Its Possible Ecophysiological Significance

1996 ◽  
Vol 76 (3) ◽  
pp. 579-594 ◽  
Author(s):  
D.W. Pond ◽  
R.P. Harris
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Stationary Phase ◽  
Stationary Phases ◽  
Stationary Growth Phase ◽  
Emiliania Huxleyi ◽  
Growth Phases ◽  
Stationary Growth ◽  
South African Isolate ◽  
Total Fatty Acids

The lipid class and fatty acid composition of eight geographically disperse isolates of Emiliania huxleyi, grown under 12 h L:D cycles and harvested during logarithmic and stationary growth phases, were examined. Cell size and chlorophyll content tended to decrease from logarithmic to stationary growth phase, Methyl and ethyl ketones were the dominant lipid classes, although proportions exhibited no clear pattern either between strains or growth phases. Neutral lipid hardly accumulated over the course of the growth experiments, and triacylglycerol was either absent or only present at low levels. In all strains with the exception of a South African isolate, levels of total fatty acid per cell decreased markedly between logarithmic and stationary phases, primarily attributable to reductions in the levels of saturated and monounsaturated fatty acids. Major fatty acids in all strains during both growth phases were 14:0,16:0,18:1 (n-9), 18:4 (n-3), 18:5 (n-3) and 22:6 (n-3). Although all strains were rich in polyunsaturated fatty acids (47–72% of total fatty acids) stationary phase cultures consistently contained the highest proportions. The polyunsaturated fatty acid docosahexanoic acid (22:6, n-3) was the most abundant fatty acid in all strains, comprising a maximum of 38·4% of total fatty acids in strain M 181 during stationary phase. Multivariate analysis (PCA) allowed logarithmic and stationary phase cultures to be distinguished although no obvious intra-isolate variability was apparent. The results are discussed in terms of the importance of lipids for the ecophysiology of E. huxleyi and the role of this dominant coccolithophore in the marine food chain.

scholarly journals Lipid and endocrine status of participants of long ski crossings in the Аrctic

2021 ◽  
Vol 17 (1) ◽  
pp. 38-43
Author(s):  
A. A. Rozumenko ◽  
L. M. Polyakov
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
North Pole ◽  
The Arctic ◽  
Insulin Content ◽  
High Density Lipoproteins ◽  
Very Low Density Lipoproteins ◽  
Male Athletes ◽  
The North ◽  
Endocrine Status

The paper presents the lipid and endocrine status of athletes — members of the two Arctic long ski crossings. The aim of the study was to study the main indicators of lipid metabolism, cortisol and insulin content in athletes during long skiing in the Arctic.Material and methods. A survey of sportsmen-­participants of two polar long ski crossings.Results. A significant decrease in the concentration of the total fraction of low and very low density lipoproteins in the blood serum was revealed in male athletes — participants of two long Arctic ski crossings. At the same time, a shift in the lipoprotein spectrum of blood towards an increase in the content of high density lipoproteins due to the second type of subfraction was revealed. The dynamics of the concentration of free fatty acids in the blood of participants in ski crossings in the Arctic was of a multidirectional nature, expressed in an increase in the level of free fatty acids compared to the initial level in the training trip and in a decrease in the level of free fatty acids at all stages of the trip to the North Pole of relative inaccessibility. During the training hike an increase in the level of cortisol in the blood was revealed, as well as an increase in the insulin content after the end of the hike. During the transition to the North Pole of relative inaccessibility a significant decrease in blood cortisol concentration compared with the preparatory period was revealed, as well as the absence of significant changes in insulin content at all stages of the transition.

GENERAL EFFECTS OF α-MELANOCYTE STIMULATING HORMONE IN THE RABBIT

1965 ◽  
Vol 48 (4) ◽  
pp. 609-618 ◽  
Author(s):  
H. K. Dyster-Aas ◽  
C. E. T. Krakau
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Serum Calcium ◽  
Calcium Level ◽  
Serum Calcium Level ◽  
Systemic Effects ◽  
List Type ◽  
Aqueous Flare ◽  
Melanocyte Stimulating Hormone ◽  
Blood Aqueous Barrier

ABSTRACT In addition to the previously described permeability disturbance in the blood aqueous barrier of the eye, measured as an increase of the aqueous flare, a series of transitory systemic effects have been recorded following the subcutaneous injection of synthetic α-MSH: marked increase of the free fatty acids in plasma, decrease in the serum calcium level, decrease in the blood pressure, increase in the skin temperature, increased frequency and diminished amplitude of respiration, presence of slow waves in the EEG. There is a correlation between the magnitude of the aqueous flare increase and the increase of free fatty acids in plasma and also between the aqueous flare and the minimum serum calcium level.

The Relationship of Fasting Free Fatty Acids, Adipose Tissue, Insulin Resistance, and Fasting Glucose Concentrations with Subsequent ß-Cell Function in Nondiabetic Subjects

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 1812-P
Author(s):  
MARIA D. HURTADO ◽  
J.D. ADAMS ◽  
MARCELLO C. LAURENTI ◽  
CHIARA DALLA MAN ◽  
CLAUDIO COBELLI ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Fatty Acids ◽  
Adipose Tissue ◽  
Free Fatty Acids ◽  
Cell Function ◽  
Fasting Glucose ◽  
Relationship Of ◽  
The Relationship

1010-P: Effects of MEDI0382, an Oxyntomodulin-Like Peptide with Targeted GLP-1/Glucagon Receptor Activity, on Amino Acids, Ketones, and Free Fatty Acids

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 1010-P
Author(s):  
VICTORIA E. PARKER ◽  
DARREN ROBERTSON ◽  
TAO WANG ◽  
DAVID C. HORNIGOLD ◽  
MAXIMILIAN G. POSCH ◽  
...  
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Free Fatty Acids ◽  
Receptor Activity ◽  
Glucagon Receptor
Sign in / Sign up

Export Citation Format

Share Document