scholarly journals Biodegradation of polycyclic aromatic hydrocarbons, phenol and sodium sulfate by Nocardia species isolated and characterized from Iranian ecosystems

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Davood Azadi ◽  
Hasan Shojaei
Keyword(s):  
Aromatic Hydrocarbons ◽  
Organic Contaminants ◽  
Environmental Samples ◽  
Pcr Amplification ◽  
Hazardous Materials ◽  
Sequencing Analysis ◽  
Nocardia Species ◽  
Anthropogenic Pollutants ◽  
Microbiological Methods ◽  
Polycyclic Aromatic

AbstractAnthropogenic pollutants are known to have adverse effect on ecosystem, biodiversity and human health. Bioremediation is an option that has been widely used to remediate organic contaminants and reduce the risk of these hazardous materials. Microorganisms are readily available to screen and can be rapidly characterized to be applied in many extreme environmental conditions. Actinomycetes have a great potential for the production of bioactive secondary metabolites which have biodegradation activity. This study aimed to screen and characterize Nocardia species with biodegradation potential from diverse Iranian ecosystems. The isolates were screened from 90 collected environmental samples, identified and characterized using conventional and molecular microbiological methods including the PCR amplification and sequencing analysis of 16S rRNA and rpoB genetic markers. Growth rate in presence of pollutants, chromatography, Gibbs and turbidometric methods were used to determine bioremediation ability. A total of 19 Nocardia isolates were recovered from the cultured samples (21.1%) that belonged to 10 various species. The most prevalent Nocardia species was N. farcinica; 4 isolates (21%), followed by N. cyriacigeorgica and N. cashijiensis like; 3 isolates each (15.7%) and N. asteroides and N. kroppenstedtii; 2 isolates each (10.5%). Our results showed that various Nocardia species have great potential for bioremediation purposes, although they have not received much attention of the scholars for such significant usage.

Biodegradation of polycyclic aromatic hydrocarbons, phenol and sodium sulfate by Nocardia species Isolated and characterized from Iranian ecosystem.

2020 ◽  
Author(s):  
Davood Azadi ◽  
Hasan Shojaei
Keyword(s):  
Aromatic Hydrocarbons ◽  
Environmental Samples ◽  
Chemical Compounds ◽  
Environmental Chemical ◽  
Nocardia Species ◽  
Chemical Pollutants ◽  
Current Concern ◽  
Biodegradation Process ◽  
Polycyclic Aromatic ◽  
Hazardous Pollutants

Abstract Background Industrial products are persistently hazardous pollutants to the environment and are of current concern to human health. Bioremediation, a process used to treat contaminated environment is an option that enhances the efficacy of the natural biodegradation process and degrade the target pollutants. Different species of bacteria have been reported for their ability to degrade pollutants to the environment. Nocardia live in diverse unfavorable environmental conditions and have a high catabolic capacity. This study aimed to screen and characterize Nocardia with bioremediation capability from Iranian diverse ecosystems. Result A total of 90 collected environmental samples were screened for Nocardia spp. The isolates were characterized based on conventional and molecular methods including sequence analysis of 16S rRNA. Growth rate in presence of pollutants, chromatography, Gibbs and turbidometric methods were used to determine bioremediation ability. The Nocardia isolates included 19 strains that belonged to 10 various species. The most prevalent Nocardia species were N. farcinica, 4 isolates (21%), followed by N. cyriacigeorgica and N. cashijiensis like, 3 isolates each (15.7%), and N. asteroids and N. kroppenstedtii 2 isolates each (10.5%). The isolates were classified in two categories based on their bioremediation ability: isolates with previously reported that have a bioremediation ability, and the isolates that showed bioremediation ability that first reported in the current study. Conclusions Our study showed that Nocardia spp. demonstrate high ability to degrade environmental chemical pollutants placing this bacteria among the best candidates for the detoxification of habitats contaminated with this chemical compounds.

scholarly journals Evidence of contamination by oil and oil products in the Santos-São Vicente estuary, São Paulo, Brazil

2012 ◽  
Vol 60 (2) ◽  
pp. 117-126 ◽  
Author(s):  
Juliana Souza Azevedo ◽  
Belisandra Lopes ◽  
Alberto Katsumiti ◽  
Elisabete Santis Braga ◽  
Helené Roche ◽  
...  
Keyword(s):  
Aromatic Hydrocarbons ◽  
Aromatic Compounds ◽  
Organic Contaminants ◽  
Brazilian Coast ◽  
Industrial Complex ◽  
Pah Metabolites ◽  
Polycyclic Aromatic ◽  
History Of ◽  
The Impact ◽  
Oil And Oil Products

Different components of the mixed function oxidase (MFO) system and the levels of fluorescent aromatic compounds in bile (FACs) were measured in Cathorops spixii in order to assess the impact of polycyclic aromatic hydrocarbons (PAHs). Fish were sampled in an estuary (Santos/São Vicente) with a history of contamination by PAHs, mainly due to the presence of the industrial complex of Cubatão city and of another of low anthropogenic influence (Cananéia) on the Brazilian coast. FACs were higher in fish from the polluted site, and the PAH 5 and 6-ring metabolites were the most frequent - with 14% and 15%, respectively. Levels of the different components of the MFO system showed the same variation profile as the FACs for both estuaries. Therefore, the values found for somatic indexes and biomarkers with data of bile PAH metabolites indicate the presence of organic contaminants, especially in the area subject to the influence of the industrial complex on the Santos/São Vicente estuary.

scholarly journals Evaluation of a Simplified Method for GC/MS Qualitative Analysis of Polycyclic Aromatic Hydrocarbons, Polychlorinated Biphenyls, and Organic Pesticides Using PARADISe Computer Program

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3727
Author(s):  
Łukasz Dąbrowski
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Polychlorinated Biphenyls ◽  
Computer Program ◽  
Aromatic Hydrocarbons ◽  
Environmental Samples ◽  
Organic Carbon Content ◽  
Standard Mixture ◽  
Polycyclic Aromatic ◽  
Organic Pesticides

For complex matrices such as environmental samples, there is usually a problem with not fully resolved peaks during GC/MS analysis. The PARADISe computer program (based on the PARFAC2 model) allows the identification of peaks using the deconvoluted mass spectra and the NIST MS library. The number of repetitions required by this software (at least five) is a real limitation for the determination of semi-volatile compounds, like polycyclic aromatic hydrocarbons, polychlorinated biphenyls, and organic pesticides in environmental samples. In this work, the method to overcome this condition was proposed and evaluated. The sets of the five files required by PARADISe were prepared by mathematically modifying the original GC/MS chromatograms obtained for the standard mixture (C = 2 µg/mL of 40 compounds) and real sample extracts (soil samples with different total organic carbon content and one cardboard extract) spiked with standards. Total average match factor for all the substances identified in a standard mixture was 874 (near 900—“excellent match”), and for all the substances in the real samples, it was 786 (near 800—“good match”). The results from PARADISe were comparable to those obtained with other programs: AMDIS (NIST) and MassHunter (Agilent), tested also in this work. PARADISe software can be effectively used for chromatogram deconvolution and substance identification.

scholarly journals Adsorption of Polycyclic Aromatic Hydrocarbons (PAHS) from Aqueous Solutions on Different Sorbents

2014 ◽  
Vol 13 (2) ◽  
pp. 87-96 ◽  
Author(s):  
Marzena Smol ◽  
Maria Włodarczyk-Makuła ◽  
Dariusz Włóka
Keyword(s):  
Aqueous Solution ◽  
Activated Carbon ◽  
Aqueous Solutions ◽  
Aromatic Hydrocarbons ◽  
Environmental Samples ◽  
Sorption Process ◽  
Mineral Sorbent ◽  
Polycyclic Aromatic ◽  
Individual Hydrocarbons ◽  
Treatment Procedures

Abstract This paper presents the results of the possibility and effectiveness of PAHs removal from a model aqueous solution, during the sorption on the selected sorbents. Six PAHs (naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene) listed by EPA for the analysis in the environmental samples were determined. Model aqueous solution was prepared with RESTEK 610 mix PAHs standard. After the sorption process, decrease in the concentration of individual hydrocarbons was observed. The removal percentage was dependent on the type of sorbent (quartz sand, mineral sorbent, activated carbon). The highest efficiency (98.1%) was observed for activated carbon.. The results shows that the sorption processes can be used in aqueous solutions treatment procedures.

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