Shared and unique metabolic features of the malignant and benign thyroid lesions determined with use of 1H HR MAS NMR spectroscopy

AbstractThe purpose of this work was to investigate the distinct and common metabolic features of the malignant and benign thyroid lesions in reference to the non-transformed tissue from the contralateral gland (chronic thyroiditis and colloid goiter). 1H HR MAS NMR spectra of 38 malignant lesions, 32 benign lesions and 112 samples from the non-tumoral tissue (32 from chronic thyroiditis and 80 samples from colloid goiter) were subjected both to multivariate and univariate analysis. The increased succinate, glutamine, glutathione, serine/cysteine, ascorbate, lactate, taurine, threonine, glycine, phosphocholine/glycerophosphocholine and decreased lipids were found in both lesion types in comparison to either colloid goiter or chronic thyroiditis. The elevated glutamate and choline, and reduced citrate and glucose were additionally evident in these lesions in reference to goiter, while the increased myo-inositol—in comparison to thyroiditis. The malignant lesions were characterized by the higher alanine and lysine levels than colloid goiter and thyroiditis, while scyllo-inositol was uniquely increased in the benign lesions (not in cancer) in comparison to both non-tumoral tissue types. Moreover, the benign lesions presented with the unique increase of choline in reference to thyroiditis (not observed in the cancerous tissue). The metabolic heterogeneity of the non-tumoral tissue should be considered in the analysis of metabolic reprogramming in the thyroid lesions.

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Thyroid lesions incidentally detected by 18F-FDG PET-CT ― a two centre retrospective study

Radiology and Oncology ◽

10.2478/raon-2014-0039 ◽

2015 ◽

Vol 49 (2) ◽

pp. 121-127 ◽

Cited By ~ 10

Author(s):

Jan Jamsek ◽

Ivana Zagar ◽

Simona Gaberscek ◽

Marko Grmek

Keyword(s):

Fdg Pet ◽

Malignant Lesion ◽

Standardized Uptake Value ◽

Benign Lesions ◽

Fdg Uptake ◽

Pet Ct ◽

Thyroid Uptake ◽

Malignant Lesions ◽

Fdg Pet Ct ◽

Thyroid Lesions

AbstractBackground. Incidental18F-FDG uptake in the thyroid on PET-CT examinations represents a diagnostic challenge. The maximal standardized uptake value (SUVmax) is one possible parameter that can help in distinguishing between benign and malignant thyroid PET lesions.Patients and methods. We retrospectively evaluated18F-FDG PET-CT examinations of 5,911 patients performed at two different medical centres from 2010 to 2011. If pathologically increased activity was accidentally detected in the thyroid, the SUVmaxof the thyroid lesion was calculated. Patients with incidental18F-FDG uptake in the thyroid were instructed to visit a thyroidologist, who performed further investigation including fine needle aspiration cytology (FNAC) if needed. Lesions deemed suspicious after FNAC were referred for surgery.Results. Incidental18F-FDG uptake in the thyroid was found in 3.89% ― in 230 out of 5,911 patients investigated on PET-CT. Malignant thyroid lesions (represented with focal thyroid uptake) were detected in 10 of 66 patients (in 15.2%). In the first medical centre the SUVmaxof 36 benign lesions was 5.6 ± 2.8 compared to 15.8 ± 9.2 of 5 malignant lesions (p < 0.001). In the second centre the SUVmaxof 20 benign lesions was 3.7 ± 2.2 compared to 5.1 ± 2.3 of 5 malignant lesions (p = 0.217). All 29 further investigated diffuse thyroid lesions were benign.Conclusions. Incidental18F-FDG uptake in the thyroid was found in 3.89% of patients who had a PET-CT examination. Only focal thyroid uptake represented a malignant lesion in our study ― in 15.2% of all focal thyroid lesions. SUVmaxshould only serve as one of several parameters that alert the clinician on the possibility of thyroid malignancy.

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Role of CD10 Marker in Differentiating Malignant Thyroid Neoplasms from Benign Thyroid Lesions (Immunohistochemical & Histopathological Study)

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2018.456 ◽

2018 ◽

Vol 6 (12) ◽

pp. 2295-2300 ◽

Cited By ~ 1

Author(s):

Samia Mohamed Gabal ◽

Mostafa Mohamed Salem ◽

Rasha Ramadan Mostafa ◽

Shaimaa Mohamed Abdelsalam

Keyword(s):

Papillary Carcinoma ◽

Lymphoblastic Leukemia ◽

Thyroid Neoplasms ◽

Lymphoid Cells ◽

Histopathological Study ◽

Benign Lesions ◽

Cd10 Expression ◽

Malignant Thyroid Neoplasms ◽

Benign Thyroid ◽

Thyroid Lesions

BACKGROUND: CD10 was initially recognised as a cell–surface antigen expressed by acute lymphoblastic leukaemias, and hence it’s early designation as Common Acute Lymphoblastic Leukemia Antigen (CALLA). Also, it has been proven to be reactive in various non-lymphoid cells and tissue and different types of neoplasms. AIM: To evaluate the immunohistochemical expression of CD10 in malignant thyroid neoplasms and different benign lesions and to assess whether CD10 can be used as a malignancy marker in thyroid pathology or not. MATERIAL AND METHODS: A total of 83 archived, formalin fixed, paraffin embedded tissue blocks of 83 cases of malignant thyroid neoplasms and different benign lesions. The samples were immunohistochemically analysed for CD10 expression. A p-value of less than 0.05 was considered statistically significant. RESULTS: CD10 was expressed in 91% of the studied malignant thyroid neoplasms and 58% of benign thyroid lesions. It was expressed in 26 of 28 (92.9%) conventional papillary carcinomas, ten of 10 (100%) follicular variants of papillary carcinoma, seven of nine (77.8%) minimally invasive follicular carcinomas, two of three (66.7%) widely invasive follicular carcinomas, and seven of 7 (100%) undifferentiated carcinomas, seven of 11 (66.7%) adenomatous nodules and eight of 15 (53.3%) follicular adenomas. No statistically significant correlations were detected between CD10 expression and patients’ age, sex, lymph node metastasis, tumour stage and capsular invasion. CONCLUSION: CD10 shows strong sensitivity (91.2%) and moderate specificity (42.3%) in the diagnosis of malignancy overall and shows strong sensitivity (86.4%) and moderate specificity (42.3%) in the diagnosis of malignancy in the follicular-patterned lesions. So, CD10 might be useful in differentiating malignant from benign thyroid lesions (good positive test) and in the diagnosis of follicular variant of papillary carcinoma.

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The reliability of fine needle aspiration cytology in the diagnosis of thyroid swelling

International Surgery Journal ◽

10.18203/2349-2902.isj20175121 ◽

2017 ◽

Vol 4 (12) ◽

pp. 3827

Author(s):

Obaid Syed ◽

Anand Auti

Keyword(s):

Needle Aspiration ◽

Benign Lesions ◽

Aspiration Cytology ◽

Medical College ◽

Malignant Case ◽

Female Predominance ◽

Thyroid Swelling ◽

Needle Aspiration Cytology ◽

Benign Thyroid ◽

Thyroid Lesions

Background: For a thyroid swelling, FNAC is the primary investigation. In most of the lesions, after excision, the Histopathology remains the same, but at times a few surprises do occur when FNAC reported benign lesions are found to be malignant on histopathology. The study aims to find reliability of FNAC in diagnosing Thyroid Lesions.Methods: This prospective study was conducted on 111 patients with thyroid swelling attending IIMSR, Medical College, Jalna during a period of two years. These patients with thyroid lesions were subjected to FNAC and then following the surgery correlated with the histopathology report.Results: Majority of the patients were in the age group of 30-50 years with female predominance. Out of 111 patients studied with FNAC, 1 sample was inadequate. Among remaining 110 cases 99 were benign thyroid lesions, 6 were malignant and 5 were highly suspicious of malignancy. HPE of thyroid specimen confirmed 101 cases as benign and 9 cases as malignant. Case in which inadequate material was obtained on FNAC was not included in study.Conclusions: FNAC is about 98.18 % reliable in diagnosing thyroid lesions. FNAC is easy, reliable and accurate investigation for all thyroid swellings.

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Thyroid Follicular Adenoma with Tracheal Stenosis

Journal of Clinical Case Studies ◽

10.16966/2471-4925.231 ◽

2021 ◽

Vol 6 (4) ◽

Author(s):

Kusunoki T ◽

Wada R

Keyword(s):

Tracheal Stenosis ◽

Malignant Tumors ◽

Follicular Adenoma ◽

Thyroid Tumor ◽

Thyroid Tumors ◽

Thyroid Lymphoma ◽

Chronic Thyroiditis ◽

Preoperative Ct ◽

Malignant Lesions ◽

Benign Thyroid

Some cases of thyroid malignant tumors and thyroid lymphoma were reported to have caused tracheal stenosis and choking. Benign thyroid tumors with dyspnea due to tracheal stenosis are very rare. We experienced a benign thyroid tumor that caused tracheal stenosis and dyspnea. In the preoperative CT, there was tracheal stenosis due to enlarged bilateral thyroid lobes and the width of the stenotic lumen was 7mm. Subtotal thyroidectomy improved the dyspnea. Postoperative histopathologic examination confirmed follicular adenoma without malignant lesions or chronic thyroiditis. On postoperative CT, the tracheal stenosis had improved and the lumen had increased to 15mm. The above findings would suggest that it should be keep in mind that even benign thyroid tumors with tracheal stenosis of less than 7mm in the lumen have the possibility of causing dyspnea.

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Grading of endometrial cancer using 1H HR-MAS NMR-based metabolomics

Scientific Reports ◽

10.1038/s41598-021-97505-y ◽

2021 ◽

Vol 11 (1) ◽

Cited By ~ 1

Author(s):

Agnieszka Skorupa ◽

Michał Poński ◽

Mateusz Ciszek ◽

Bartosz Cichoń ◽

Mateusz Klimek ◽

...

Keyword(s):

Endometrial Cancer ◽

Statistical Significance ◽

Univariate Analysis ◽

Magic Angle Spinning ◽

Mas Nmr ◽

Metabolic Reprogramming ◽

Magic Angle ◽

Isoleucine Leucine ◽

Glycine Metabolism ◽

Angle Spinning

AbstractThe tissue metabolomic characteristics associated with endometrial cancer (EC) at different grades were studied using high resolution (400 MHz) magic angle spinning (HR-MAS) proton spectroscopy. The metabolic profiles were obtained from 64 patients (14 with grade 1 (G1), 33 with grade 2 (G2) and 17 with grade 3 (G3) tumors) and compared with the profile acquired from 10 patients with the benign disorders. OPLS-DA revealed increased valine, isoleucine, leucine, hypotaurine, serine, lysine, ethanolamine, choline and decreased creatine, creatinine, glutathione, ascorbate, glutamate, phosphoethanolamine and scyllo-inositol in all EC grades in reference to the non-transformed tissue. The increased levels of taurine was additionally detected in the G1 and G2 tumors in comparison to the control tissue, while the elevated glycine, N-acetyl compound and lactate—in the G1 and G3 tumors. The metabolic features typical for the G1 tumors are the increased dimethyl sulfone, phosphocholine, and decreased glycerophosphocholine and glutamine levels, while the decreased myo-inositol level is characteristic for the G2 and G3 tumors. The elevated 3-hydroxybutyrate, alanine and betaine levels were observed in the G3 tumors. The differences between the grade G1 and G3 malignances were mainly related to the perturbations of phosphoethanolamine and phosphocholine biosynthesis, inositol, betaine, serine and glycine metabolism. The statistical significance of the OPLS-DA modeling was also verified by an univariate analysis. HR-MAS NMR based metabolomics provides an useful insight into the metabolic reprogramming in endometrial cancer.

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Sensitivity gains, linearity, and spectral reproducibility in nonuniformly sampled multidimensional MAS NMR spectra of high dynamic range

Journal of Biomolecular NMR ◽

10.1007/s10858-014-9824-4 ◽

2014 ◽

Vol 59 (2) ◽

pp. 57-73 ◽

Cited By ~ 26

Author(s):

Christopher L. Suiter ◽

Sivakumar Paramasivam ◽

Guangjin Hou ◽

Shangjin Sun ◽

David Rice ◽

...

Keyword(s):

Nmr Spectra ◽

Dynamic Range ◽

High Dynamic Range ◽

Mas Nmr ◽

High Dynamic

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Hydration effects in the carbon-13 CP/MAS NMR spectra of solid (1 →3)-β-D-glucans

Macromolecules ◽

10.1021/ma00133a046 ◽

1984 ◽

Vol 17 (3) ◽

pp. 501-502 ◽

Cited By ~ 20

Author(s):

C. A. Fyfe ◽

P. J. Stephenson ◽

M. G. Taylor ◽

T. L. Bluhm ◽

Y. Deslandes ◽

...

Keyword(s):

Nmr Spectra ◽

Mas Nmr ◽

Cp Mas Nmr

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Blood Oxygenation Level Dependent Magnetic Resonance Imaging (MRI), Dynamic Contrast Enhanced MRI and Diffusion Weighted MRI for Benign and Malignant Breast Cancer Discrimination: A Preliminary Experience

Cancers ◽

10.3390/cancers13102421 ◽

2021 ◽

Vol 13 (10) ◽

pp. 2421

Author(s):

Roberta Fusco ◽

Vincenza Granata ◽

Mauro Mattace Raso ◽

Paolo Vallone ◽

Alessandro Pasquale De Rosa ◽

...

Keyword(s):

Standard Deviation ◽

Univariate Analysis ◽

Mean Value ◽

Blood Oxygenation ◽

Breast Lesions ◽

Dce Mri ◽

Malignant Lesions ◽

The Mean ◽

And Diffusion ◽

Skewness And Kurtosis

Purpose. To combine blood oxygenation level dependent magnetic resonance imaging (BOLD-MRI), dynamic contrast enhanced MRI (DCE-MRI), and diffusion weighted MRI (DW-MRI) in differentiation of benign and malignant breast lesions. Methods. Thirty-seven breast lesions (11 benign and 21 malignant lesions) pathologically proven were included in this retrospective preliminary study. Pharmaco-kinetic parameters including Ktrans, kep, ve, and vp were extracted by DCE-MRI; BOLD parameters were estimated by basal signal S0 and the relaxation rate R2*; and diffusion and perfusion parameters were derived by DW-MRI (pseudo-diffusion coefficient (Dp), perfusion fraction (fp), and tissue diffusivity (Dt)). The correlation coefficient, Wilcoxon-Mann-Whitney U-test, and receiver operating characteristic (ROC) analysis were calculated and area under the ROC curve (AUC) was obtained. Moreover, pattern recognition approaches (linear discrimination analysis and decision tree) with balancing technique and leave one out cross validation approach were considered. Results. R2* and D had a significant negative correlation (−0.57). The mean value, standard deviation, Skewness and Kurtosis values of R2* did not show a statistical significance between benign and malignant lesions (p > 0.05) confirmed by the ‘poor’ diagnostic value of ROC analysis. For DW-MRI derived parameters, the univariate analysis, standard deviation of D, Skewness and Kurtosis values of D* had a significant result to discriminate benign and malignant lesions and the best result at the univariate analysis in the discrimination of benign and malignant lesions was obtained by the Skewness of D* with an AUC of 82.9% (p-value = 0.02). Significant results for the mean value of Ktrans, mean value, standard deviation value and Skewness of kep, mean value, Skewness and Kurtosis of ve were obtained and the best AUC among DCE-MRI extracted parameters was reached by the mean value of kep and was equal to 80.0%. The best diagnostic performance in the discrimination of benign and malignant lesions was obtained at the multivariate analysis considering the DCE-MRI parameters alone with an AUC = 0.91 when the balancing technique was considered. Conclusions. Our results suggest that the combined use of DCE-MRI, DW-MRI and/or BOLD-MRI does not provide a dramatic improvement compared to the use of DCE-MRI features alone, in the classification of breast lesions. However, an interesting result was the negative correlation between R2* and D.

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TAMI-06. PRECLINICAL MODELS REVEAL BRAIN-MICROENVIRONMENT SPECIFIC METABOLIC DEPENDENCIES IN GLIOBLASTOMA

Neuro-Oncology ◽

10.1093/neuonc/noaa215.895 ◽

2020 ◽

Vol 22 (Supplement_2) ◽

pp. ii214-ii214

Author(s):

Jenna Minami ◽

Nicholas Bayley ◽

Christopher Tse ◽

Henan Zhu ◽

Danielle Morrow ◽

...

Keyword(s):

Tumor Microenvironment ◽

Cultured Cells ◽

Tumor Biology ◽

Metabolic Reprogramming ◽

Neoplastic Progression ◽

Extracellular Milieu ◽

Metabolic Defects ◽

Metabolic Heterogeneity

Abstract Metabolic reprogramming is a hallmark of cancer, and malignant cells must acquire metabolic adaptations to fuel neoplastic progression. Mutations or changes in metabolic gene expression can impose nutrient dependencies in tumors, and even in the absence of metabolic defects, cancer cells can become auxotrophic for particular nutrients or metabolic byproducts generated by other cells in the tumor microenvironment (TME). Conventional cell lines do not recapitulate the metabolic heterogeneity of glioblastoma (GBM), while primary cultured cells do not account for the influences of the microenvironment and the blood brain barrier on tumor biology. Additionally, these systems are under strong selective pressure divergent from that in vivo, leading to reduced heterogeneity between cultured tumor cells. Here, we describe a biobank of direct-from-patient derived orthotopic xenografts (GliomaPDOX) and gliomaspheres that reveal a subset of gliomas that, while able to form in vivo, cannot survive in vitro. RNA sequencing of tumors that can form both in vivo and in vitro (termed “TME-Indifferent”) compared to that of tumors that can only form in vivo (termed “TME-Dependent”) revealed transcriptional changes associated with altered nutrient availability, emphasizing the unique metabolic programs impacted by the tumor microenvironment. Furthermore, TME-dependent tumors lack metabolic signatures associated with nutrient biosynthesis, thus indicating a potential dependency of these tumors on scavenging specific nutrients from the extracellular milieu. Collectively, these data emphasize the metabolic heterogeneity within GBM, and reveal a subset of gliomas that lack metabolic plasticity, indicating a potential brain-microenvironment specific metabolic dependency that can be targeted for therapy.

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The Application of Fine Needle Aspiration Biopsy in the Diagnosis of Axillary Masses

Acta Cytologica ◽

10.1159/000513149 ◽

2021 ◽

pp. 1-7

Author(s):

Cong-Gai Huang ◽

Meng-Ze Li ◽

Shao-Hua Wang ◽

Xiao-Qin Tang ◽

Johannes Haybaeck ◽

...

Keyword(s):

Lymph Node ◽

Fine Needle Aspiration ◽

Predictive Value ◽

Fine Needle Aspiration Biopsy ◽

Needle Aspiration ◽

Benign Lesions ◽

Fine Needle ◽

Significant Difference ◽

Malignant Lesions ◽

Axillary Mass

Introduction: We intend to determine the diagnostic power of fine needle aspiration biopsy (FNAB) for differentiation between malignant and benign lesions on axillary masses and draw the physicians’ attention to the benefits of FNAB cytology in the diagnosis of axillary masses. Methods: In this study, 1,328 patients with an axillary mass diagnosed by FNAB were retrospectively reviewed. These cases were registered at the affiliated hospital of Southwest Medical University (China), July 2014 to June 2017. Cytological results were verified either by histopathology following surgical resection or clinical follow-up. Results: Of the 1,328 patients affected by axillary masses, 987 (74.3%) cases were female, and 341 (25.7%) cases were male. The highest incidence of patients was in the age group of 41–50 years (375, 28.2%). There were 1,129 (85.0%) patients with benign lesions and 199 (15.0%) with malignant lesions. Of the 199 malignant lesions cases, 21 cases were lymphomas, 2 cases were accessory breast cancers, and 176 cases were lymph node metastatic tumors. Under lymph node metastases, the most frequent primary tumors were breast cancer (141, 80.1%), followed by lung cancer (21, 11.9%). According to the study, the characters of 1,328 cases showed statistically significant difference (χ2 = 4.534, p = 0.033), and the incidence of females with axillary mass was significantly higher than that of males. There was a statistically significant difference in the distribution of benign and malignant cases in the patient age groups (χ2 = 1.129, p = 0.000), and the incidence of patients of 41–50 years of age was significantly higher than that of other patients. The diagnostic accuracy of FNAB in axillary masses was analyzed with the results of 95.98% of sensitivity, 99.56% of specificity, 97.45% of positive predictive value, and 99.29% of negative predictive value. Conclusion: Our results confirm that FNAB is a valuable initial screening method regarding pathologic diagnosis of axillary mass, in particular with respect to malignancy in 41- to 50-year-old female patients.

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