scholarly journals The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jacek Francikowski ◽  
Marta Potrzebska ◽  
Elżbieta Szulińska ◽  
Monika Tarnawska ◽  
Zoltan Radai ◽  
...  

AbstractTryptophan (TRP) is one of the essential amino acids in the animal body. Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level. It has been shown to have a number of essential functions, such as in the production of other biologically active molecules. The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect—house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)—white eye. This study was aimed at determining the effects of glucose and fructose on cricket development and biochemical composition. A parallel goal was to compare the response of both cricket strains to HMD. ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio. Biochemical analyses demonstrated the effects of HMD mainly on fat and glycogen content. A decrease in food intake was also observed in the groups on HMD. However, no changes in imago body weight and water content were observed. The results of the study indicate a stronger response of the white strain to HMD compared to the wild-type strain. At the same time, a stronger detrimental effect of fructose than of glucose was apparent. Sex was found to be a modulating factor in the response to HMD.

Author(s):  
J. R. Adams ◽  
G. J Tompkins ◽  
A. M. Heimpel ◽  
E. Dougherty

As part of a continual search for potential pathogens of insects for use in biological control or on an integrated pest management program, two bacilliform virus-like particles (VLP) of similar morphology have been found in the Mexican bean beetle Epilachna varivestis Mulsant and the house cricket, Acheta domesticus (L. ).Tissues of diseased larvae and adults of E. varivestis and all developmental stages of A. domesticus were fixed according to procedures previously described. While the bean beetles displayed no external symptoms, the diseased crickets displayed a twitching and shaking of the metathoracic legs and a lowered rate of activity.Examinations of larvae and adult Mexican bean beetles collected in the field in 1976 and 1977 in Maryland and field collected specimens brought into the lab in the fall and reared through several generations revealed that specimens from each collection contained vesicles in the cytoplasm of the midgut filled with hundreds of these VLP's which were enveloped and measured approximately 16-25 nm x 55-110 nm, the shorter VLP's generally having the greater width (Fig. 1).


1962 ◽  
Vol 40 (3) ◽  
pp. 371-374 ◽  
Author(s):  
Claude Ritchot ◽  
J. E. McFarlane

Lipid other than sterol improves the growth and development of nymphs of Acheta domesticus (L.), as shown by the effect of adding wheat germ oil and linoleic acid to the diet. Such lipid is also necessary for reproduction: the effect appears to be on the male, but may also be on the female.


Author(s):  
J.M. Sorjonen ◽  
M. Karhapää ◽  
S. Holm ◽  
A. Valtonen ◽  
H. Roininen

As insect farming is becoming more popular in Europe and in the US, the use of food industry by-products in insects’ feed is drawing more attention. However, it is still unclear how to maximise the use of by-products in the feeds while meeting the nutritional requirements and maintaining profitable rearing yields with the current production technology. In this study, we conducted an experiment in Finnish small-scale cricket farming and laboratory conditions to determine whether five diet treatments, including by-product diets with barley mash and turnip rape, modify the overall fresh yield (kg), development time and feed conversion rate of the house crickets (Acheta domesticus). We discovered that the diets differed in the overall fresh cricket yield (kg) but not in the development time or the feed conversion rate of the crickets. The diets with a high proportion of by-product decreased the price of the feed but at the same time reduced the yield. Based on our results, complex by-product diets with the by-product proportion of 20-41% are the most suitable for house cricket rearing.


Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2680
Author(s):  
Sathita Areerat ◽  
Pipatpong Chundang ◽  
Chalermpol Lekcharoensuk ◽  
Attawit Kovitvadhi

There has been increasing interest in using insects as sustainable protein sources for humans and animals. Therefore, this study aimed to explore the possibility of substituting poultry meal with house cricket (Acheta domesticus: AD) or mulberry silkworm (Bombyx mori: BM) pupae. Fifty healthy adult mixed-breed dogs were selected and divided into five experimental groups, which were fed, in amounts based on daily energy requirement, with a control diet, a diet with 10% AD, with 20% AD, with 7% BM, or with 14% BM. Days 0–22 and 23–28 of the experiment served as the adaptation and collection phases, respectively. Haematology and blood chemistry were collected at days 0, 14, and 28, and body weight, body condition score, feed intake, faecal output, faecal score, faecal moisture, and apparent total tract digestibility of dry matter and nutrients were measured during the collection phase. The results from this study suggested that AD and BM can replace poultry meal without any adverse consequences on all measured parameters (p > 0.05). Therefore, AD at 20% or BM at 14% can be used in canine diet formulations. However, long-term feeding trials should be conducted and should focus on clinical signs relating to hypersensitivity disorders.


2021 ◽  
pp. 245-252
Author(s):  
Sergey Aleksandrovich Makarenko

A comparative analysis of the varietal variability of the fruits of 71 varieties of apple trees of Altai selection in terms of biochemical composition, according to generally accepted methods using statistical variation analysis based on long-term research, was carried out. Correlations were revealed between the content of chemical elements depending on other characteristics of the fruit and the period of ripening. The average content of soluble dry matter in fruits is from an average 15.5% in summer varieties to a high 16.2–16.3% in varieties with autumn and winter ripening. On average, the content of total sugars in fruits is high and varies insignificantly from 12.7 to 12.9%, the content of titratable acids in fruits is high – 1.0%. The content of ascorbic acid is higher in fruits of varieties with summer ripening and is 24.0 mg / 100 g, with autumn – 19.6 mg / 100 g, with winter – 22.0 mg / 100 g. As a result of selection for the qualitative improvement of fruits apple trees in Altai revealed a negative correlation of the biochemistry of the fetus, depending on its size for all indicators. Fruits of a significant part of varieties created in Altai have good taste, of which 27 have a sugar-acid index of 15–25. The fruits of the varieties Alpek, Altai Velvet, Altai New Year, Altai ruddy, Altai sweet, Gornoaltayskoe, Zolotaya taiga, Pepinka Altai have a very high sugar content of more than 15%, a high value of the sum of sugars – in fruits of 59 varieties from 10.0 to 14.9%. Varieties with high biochemical characteristics are their sources in breeding for improved biochemical composition of fruits.


1958 ◽  
Vol s3-99 (48) ◽  
pp. 453-457
Author(s):  
BLANCHE-P CLAYTON

1. When the testis of Acheta domesticus is fixed in Flemming's fluid and embedded in gelatine, Sudan black reveals scarcely any lipid in the externum of the acroblast. 2. It can be shown that the externum of the acroblast contains much lipid in a masked (bound) form. 3. Experiments were performed to find what substances were effective, after Flemming fixation, in unmasking the lipid of the externum and thus making it colourable by Sudan black. 4. The following substances were found to act as unmasking agents: ethanol, dioxane, phenol, resorcinol, hydroquinone, pyrogallol, tannic acid. 5. Under the conditions of the experiment, the most effective unmasking agents were 90% ethanol and 5% hydroquinone.


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