Short high fat diet triggers reversible and region specific effects in DCX+ hippocampal immature neurons of adolescent male mice

AbstractAdolescence represents a crucial period for maturation of brain structures involved in cognition. Early in life unhealthy dietary patterns are associated with inferior cognitive outcomes at later ages; conversely, healthy diet is associated with better cognitive results. In this study we analyzed the effects of a short period of hypercaloric diet on newborn hippocampal doublecortin+ (DCX) immature neurons in adolescent mice. Male mice received high fat diet (HFD) or control low fat diet (LFD) from the 5th week of age for 1 or 2 weeks, or 1 week HFD followed by 1 week LFD. After diet supply, mice were either perfused for immunohistochemical (IHC) analysis or their hippocampi were dissected for biochemical assays. Detailed morphometric analysis was performed in DCX+ cells that displayed features of immature neurons. We report that 1 week-HFD was sufficient to dramatically reduce dendritic tree complexity of DCX+ cells. This effect occurred specifically in dorsal and not ventral hippocampus and correlated with reduced BDNF expression levels in dorsal hippocampus. Both structural and biochemical changes were reversed by a return to LFD. Altogether these studies increase our current knowledge on potential consequences of hypercaloric diet on brain and in particular on dorsal hippocampal neuroplasticity.

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Normal diet Vs High fat diet - A comparative study: Behavioral and neuroimmunological changes in adolescent male mice.

Metabolic Brain Disease ◽

10.1007/s11011-017-0140-z ◽

2017 ◽

Vol 33 (1) ◽

pp. 177-190 ◽

Cited By ~ 24

Author(s):

Huali Wu ◽

Qiongzhen Liu ◽

Praveen Kumar Kalavagunta ◽

Qiaoling Huang ◽

Wenting Lv ◽

...

Keyword(s):

Comparative Study ◽

High Fat Diet ◽

Normal Diet ◽

Adolescent Male ◽

Male Mice ◽

High Fat

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Effect of Aerobic Training and High-fat Diet on Enos and Ros in Testicular Tissue of Juvenile Male Rats

Jundishapur Journal of Medical Sciences ◽

10.32598/jsmj.20.3.2045 ◽

2021 ◽

Vol 20 (3) ◽

pp. 280-289

Author(s):

Parisa Norouzzadeh ◽

◽

Roghayeh Pouzesh Jadidi ◽

Keyword(s):

Aerobic Exercise ◽

High Fat Diet ◽

Aerobic Training ◽

Testicular Tissue ◽

Normal Diet ◽

Male Rats ◽

Adolescent Male ◽

Male Mice ◽

High Fat ◽

Diet Control

Background and Objectives: This study aimed to determine the effect of a course of aerobic exercise with a high-fat diet on eNOS and ROS in testicular tissue of adolescent male rats. Subjects and Methods A total of 40 adolescent male rats (30 days old) were randomized in the following groups: normal diet control, normal diet training, high fat diet control, and high-fat diet training. The high-fat diet rats were under a high-fat regimen (5.817 kcal/g) for 30 days, and then a normal fat diet (3.801 kcal/g) was continued after the 60th day of birth. Aerobic training was conducted for four weeks included three training sessions from the 70th to 98th days of life. Results The results showed that the amount of ROS in the testicular tissue of male mice was higher only in the high-fat diet group. Also, there was no significant difference between the groups regarding eNOS testicular tissue in male mice. Conclusion A high-fat diet increases the production of reactive oxygen species in testicular tissue and is not affected by aerobic exercise. Also, neither exercise nor a high-fat diet had any effect on testicular eNOS. However, due to the limitations of this study and no evidence in this field, further studies are needed on cell phenotype, sperm fate, and identification of pathways involved in the occurrence of oxidative stress and subsequent effects of eNOS activation in testicular tissue in response to exercise and obesity.

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Thrombospondin 1 Mediates High-Fat Diet-Induced Muscle Fibrosis and Insulin Resistance in Male Mice

Endocrinology ◽

10.1210/en.2013-1587 ◽

2013 ◽

Vol 154 (12) ◽

pp. 4548-4559 ◽

Cited By ~ 43

Author(s):

Mayumi Inoue ◽

Yibin Jiang ◽

Richard H. Barnes ◽

Masakuni Tokunaga ◽

Gabriel Martinez-Santibañez ◽

...

Keyword(s):

Insulin Resistance ◽

High Fat Diet ◽

Skeletal Muscles ◽

Matrix Protein ◽

Extracellular Matrix Protein ◽

Male Mice ◽

High Fat ◽

Adipose Tissues ◽

Early Stages ◽

Thrombospondin 1

Thrombospondin 1 (THBS1 or TSP-1) is a circulating glycoprotein highly expressed in hypertrophic visceral adipose tissues of humans and mice. High-fat diet (HFD) feeding induces the robust increase of circulating THBS1 in the early stages of HFD challenge. The loss of Thbs1 protects male mice from diet-induced weight gain and adipocyte hypertrophy. Hyperinsulinemic euglycemic clamp study has demonstrated that Thbs1-null mice are protected from HFD-induced insulin resistance. Tissue-specific glucose uptake study has revealed that the insulin-sensitive phenotype of Thbs1-null mice is mostly mediated by skeletal muscles. Further assessments of the muscle phenotype using RNA sequencing, quantitative PCR, and histological studies have demonstrated that Thbs1-null skeletal muscles are protected from the HFD-dependent induction of Col3a1 and Col6a1, coupled with a new collagen deposition. At the same time, the Thbs1-null mice display a better circadian rhythm and higher amplitude of energy expenditure with a browning phenotype in sc adipose tissues. These results suggest that THBS1, which circulates in response to a HFD, may induce insulin resistance and fibrotic tissue damage in skeletal muscles as well as the de-browning of sc adipose tissues in the early stages of a HFD challenge. Our study may shed new light on the pathogenic role played by a circulating extracellular matrix protein in the cross talk between adipose tissues and skeletal muscles during obesity progression.

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Hypoglycemic effects of Auricularia auricula polysaccharides on high fat diet and streptozotocin-induced diabetic mice using metabolomics analysis

Food & Function ◽

10.1039/d1fo02022f ◽

2021 ◽

Author(s):

nannan liu ◽

Xuefeng Chen ◽

Juanna Song ◽

Mengyin Chen ◽

Pin Gong ◽

...

Keyword(s):

Diabetes Mellitus ◽

High Fat Diet ◽

Diabetic Mice ◽

Male Mice ◽

High Fat ◽

Auricularia Auricula ◽

Ultrahigh Performance Liquid Chromatography ◽

Metabolomic Approach ◽

Metabolomics Analysis

This study evaluated the hypoglycemic effect of Auricularia auricula polysaccharides (AAPs) on streptozotocin-induced type 2 diabetes mellitus (T2DM) male mice (C57BL/6J) using a metabolomic approach based on ultrahigh-performance liquid chromatography–Q...

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Influence of gender on OVA-induced airway inflammation in C57/B6J mice on a high-fat diet

European Journal of Inflammation ◽

10.1177/2058739218760946 ◽

2018 ◽

Vol 16 ◽

pp. 205873921876094 ◽

Cited By ~ 1

Author(s):

Gang Yu ◽

Lili Zhu ◽

Haiyan Li ◽

Youyou Shao ◽

Lei Chong ◽

...

Keyword(s):

Body Weight ◽

High Fat Diet ◽

Inflammatory Cells ◽

Normal Weight ◽

Male Mice ◽

High Fat ◽

Low Fat ◽

Control Male ◽

Low Fat Diet ◽

Asthma Group

Overweight/obesity has been suggested as a risk factor for asthma development, and prospective studies have confirmed that high body weight precedes asthma symptoms. However, the nature of the association between overweight/obese status and asthma remains unclear. Animal models of obesity-related asthma are very useful for understanding disease pathophysiology. Although C57/B6J mice are the most widely used animal model for researching obesity-related asthma, gender differences are not always taken into consideration. Therefore, to explore the effect of gender on the development of obesity-related asthma, both female and male C57/B6J mice were used in this study. The mice were fed with a high-fat diet or a low-fat diet as control. Body weight, body length, liver weight, and Lee’s Index were used to evaluate obesity status, and lung histology, lung inflammatory cells infiltration, and inflammatory cytokines in bronchoalveolar lavage fluid (BALF) were examined for asthma evaluation. We found that the mean body weight of male mice on a high-fat diet gradually increased and was significantly higher than control male mice on a low-fat diet ( P < 0.01), while no significant differences were found between female mice at the end of 12 weeks of feeding. Furthermore, the obese asthma group female and male mice exhibited significantly high inflammatory cells infiltration than normal weight or obese female and male mice ( P < 0.01). However, the obese asthma group presented higher Neu infiltration, Th1 cytokine, and interferon gamma (IFNγ) concentrations in BALF than the asthma group in both the genders ( P < 0.01). In conclusion, both female and male mice are suitable for the obesity-related asthma model, although male mice might be more stable. Besides, obesity-related asthma is not Th2 type asthma.

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Androgen Receptor-Dependent and Independent Atheroprotection by Testosterone in Male Mice

Endocrinology ◽

10.1210/en.2010-0663 ◽

2010 ◽

Vol 151 (11) ◽

pp. 5428-5437 ◽

Cited By ~ 58

Author(s):

Johan Bourghardt ◽

Anna S. K. Wilhelmson ◽

Camilla Alexanderson ◽

Karel De Gendt ◽

Guido Verhoeven ◽

...

Keyword(s):

Androgen Receptor ◽

Apolipoprotein E ◽

High Fat Diet ◽

Atherosclerotic Lesion ◽

Male Mice ◽

Wild Type ◽

High Fat ◽

Lesion Area ◽

Major Pathway

The atheroprotective effect of testosterone is thought to require aromatization of testosterone to estradiol, but no study has adequately addressed the role of the androgen receptor (AR), the major pathway for the physiological effects of testosterone. We used AR knockout (ARKO) mice on apolipoprotein E-deficient background to study the role of the AR in testosterone atheroprotection in male mice. Because ARKO mice are testosterone deficient, we sham operated or orchiectomized (Orx) the mice before puberty, and Orx mice were supplemented with placebo or a physiological testosterone dose. From 8 to 16 wk of age, the mice consumed a high-fat diet. In the aortic root, ARKO mice showed increased atherosclerotic lesion area (+80%, P < 0.05). Compared with placebo, testosterone reduced lesion area both in Orx wild-type (WT) mice (by 50%, P < 0.001) and ARKO mice (by 24%, P < 0.05). However, lesion area was larger in testosterone-supplemented ARKO compared with testosterone-supplemented WT mice (+57%, P < 0.05). In WT mice, testosterone reduced the presence of a necrotic core in the plaque (80% among placebo-treated vs. 12% among testosterone-treated mice; P < 0.05), whereas there was no significant effect in ARKO mice (P = 0.20). In conclusion, ARKO mice on apolipoprotein E-deficient background display accelerated atherosclerosis. Testosterone treatment reduced atherosclerosis in both WT and ARKO mice. However, the effect on lesion area and complexity was more pronounced in WT than in ARKO mice, and lesion area was larger in ARKO mice even after testosterone supplementation. These results are consistent with an AR-dependent as well as an AR-independent component of testosterone atheroprotection in male mice.

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Anti-Obesity Effects of Combined Cornus officinalis and Ribes fasciculatum Extract in High-Fat Diet-Induced Obese Male Mice

Animals ◽

10.3390/ani11113187 ◽

2021 ◽

Vol 11 (11) ◽

pp. 3187

Author(s):

Eunkuk Park ◽

Chang-Gun Lee ◽

Hyoju Jeon ◽

Hyesoo Jeong ◽

Subin Yeo ◽

...

Keyword(s):

High Fat Diet ◽

Insulin Action ◽

Normal Diet ◽

Male Mice ◽

Impaired Glucose Metabolism ◽

High Fat ◽

Beneficial Effects ◽

Cornus Officinalis ◽

Treatment And Prevention ◽

Obese Male

Medicinal plants are widely used as supplements for the treatment of various diseases because of their few side-effects. Here, we examined the anti-obesity effects of a mixture extract of Cornus officinalis and Ribes fasciculatum (CR) in high-fat diet (HFD)-induced obese male mice. Four week old male C57BL/6J mice were fed a normal diet (ND) or 60% high-fat diet (HFD) with different concentrations of CR extracts (75, 150, and 300 mg/kg/day) by oral administration for 12 weeks. CR extract administration prevented HFD-induced weight gain, hepatic steatosis, and adipocyte enlargement through the downregulation of adipogenesis-associated genes in obese male mice. In addition, CR administration improved the impaired glucose metabolism, insulin action, biochemical obesity parameters, and metabolic profiles in HFD-induced male mice. Consequently, the CR extract exhibited beneficial effects on HFD-induced systemic metabolic challenges. Taken together, our findings suggest that CR extract may be a potent therapeutic supplement for the treatment and prevention of obesity.

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Effect of high fat diet on inflammatory markers and bone morphological microstructure in growing male mice

The FASEB Journal ◽

10.1096/fasebj.26.1_supplement.650.3 ◽

2012 ◽

Vol 26 (S1) ◽

Author(s):

Misung Kim ◽

Woori Na ◽

Hanlla Kim ◽

Eunhye Park ◽

Hyun-A Lee ◽

...

Keyword(s):

High Fat Diet ◽

Inflammatory Markers ◽

Male Mice ◽

High Fat

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Microglia Regulate Neuronal Circuits in Homeostatic and High-Fat Diet-Induced Inflammatory Conditions

Frontiers in Cellular Neuroscience ◽

10.3389/fncel.2021.722028 ◽

2021 ◽

Vol 15 ◽

Author(s):

Xiao-Lan Wang ◽

Lianjian Li

Keyword(s):

High Fat Diet ◽

Microglial Activation ◽

Current Knowledge ◽

Brain Diseases ◽

High Fat ◽

Synaptic Remodeling ◽

Inflammatory Conditions ◽

Cellular Debris ◽

Neuronal Connections ◽

Almost All

Microglia are brain resident macrophages, which actively survey the surrounding microenvironment and promote tissue homeostasis under physiological conditions. During this process, microglia participate in synaptic remodeling, neurogenesis, elimination of unwanted neurons and cellular debris. The complex interplay between microglia and neurons drives the formation of functional neuronal connections and maintains an optimal neural network. However, activation of microglia induced by chronic inflammation increases synaptic phagocytosis and leads to neuronal impairment or death. Microglial dysfunction is implicated in almost all brain diseases and leads to long-lasting functional deficiency, such as hippocampus-related cognitive decline and hypothalamus-associated energy imbalance (i.e., obesity). High-fat diet (HFD) consumption triggers mediobasal hypothalamic microglial activation and inflammation. Moreover, HFD-induced inflammation results in cognitive deficits by triggering hippocampal microglial activation. Here, we have summarized the current knowledge of microglial characteristics and biological functions and also reviewed the molecular mechanism of microglia in shaping neural circuitries mainly related to cognition and energy balance in homeostatic and diet-induced inflammatory conditions.

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