scholarly journals Effects of integrated biocontrol on bacterial wilt and rhizosphere bacterial community of tobacco

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yun Hu ◽  
Yanyan Li ◽  
Xiaoqiong Yang ◽  
Chunli Li ◽  
Lin Wang ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Physicochemical Properties ◽  
Rice Bran ◽  
Bacterial Wilt ◽  
Community Diversity ◽  
Antagonistic Bacteria ◽  
Soil Physicochemical Properties ◽  
Long Term Effects ◽  
Calcium Cyanamide ◽  
Colonization Ability

AbstractBacterial wilt as a soil-borne disease was caused by Ralstonia solanacearum, and seriously damages the growth of tobacco. Integrated biocontrol method was explored to control bacterial wilt. Nevertheless, the long-term effects of the integrated biocontrol method on soil bacterial community, soil physicochemical properties and the incidence of bacterial wilt are not well understood. In this study, B. amyoliquefaciens ZM9, calcium cyanamide and rice bran were applied to tobacco fields in different ways. The disease index and incidence of tobacco bacterial wilt (TBW), soil physicochemical properties, colonization ability of B. amyoliquefaciens ZM9, and rhizopshere bacterial community were investigated. The results showed that the integrated application of B. amyoliquefaciens ZM9, rice bran and calcium cyanamide had the highest control efficiency of TBW and bacteria community diversity. Additionally, the integrated biocontrol method could improve the colonization ability of B. amyoliquefaciens ZM9. Furthermore, the integrated biocontrol method could effectively suppress TBW by regulating soil physicochemical properties, promoting beneficial bacteria and antagonistic bacteria of rhizopshere soil. This strategy has prospect of overcoming the defects in application of a single antagonistic bacteria and provides new insights to understand how to improve the colonization capacity of antagonistic bacteria and control efficacy for TBW.

scholarly journals Integrated biocontrol of tobacco bacterial wilt by antagonistic bacteria and marigold

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yun Hu ◽  
Wan Zhao ◽  
Xihong Li ◽  
Ji Feng ◽  
Chunli Li ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Bacterial Wilt ◽  
Rhizosphere Soil ◽  
Synergistic Effects ◽  
Inhibitory Effect ◽  
Integrated Treatment ◽  
Antagonistic Bacteria ◽  
Soil Physicochemical Properties ◽  
Microbial Composition ◽  
Effective Measure

AbstractTobacco bacterial wilt (TBW) is seriously damages the growth of tobacco. There is an urgent need to find a safer and more effective measure to control TBW. In this study, B. amyloliquefaciens ZM9 and marigold powder were applied to the tobacco roots alone or in combination, and the potential inhibition of TBW was assessed. On the other hand, the effects of these treatments on soil physicochemical properties, rhizosphere microbial community and soil metabolites were also evaluated. The results showed that the application of B. amyloliquefaciens ZM9 or marigold powder alone significantly reduced the abundance of R. solanacearum in rhizosphere soil, while the integrated treatment showed the strongest inhibitory effect. Moreover, the integrated treatment can inhibit the secretion of chemoattractants, and affect the change of rhizosphere soil microbial composition. In conclusion, the combination of antagonistic bacteria agent B. amyloliquefaciens ZM9 with marigold powder can enhance the suppression of TBW. Furthermore, B. amyloliquefaciens ZM9 and marigold have synergistic effects on suppressing TBW by regulation soil physicochemical properties, soil metabolites and microbial structure. This study provide a promising strategy for TBW control by integrated applying of B. amyloliquefaciens ZM9 and marigold powder.

scholarly journals Variation of rhizosphere bacterial community diversity in the desert ephemeral plant Ferula sinkiangensis across environmental gradients

2019 ◽  
Author(s):  
Zhang Tao ◽  
Dang Han Li ◽  
Wang Zhong Ke ◽  
Lv Xin Hua ◽  
Zhuang Li
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Physicochemical Properties ◽  
Bacterial Community Structure ◽  
Soil Depth ◽  
Community Diversity ◽  
Slope Position ◽  
Soil Physicochemical Properties ◽  
Rhizosphere Bacterial Community ◽  
Ferula Sinkiangensis

Abstract Background Ferula sinkiangensis is a desert short-lived medicinal plant, and its number is rapidly decreasing. Rhizosphere microbial community plays an important role in regulating global biogeochemical cycle, plant growth and adaptability. However, the Ferula sinkiangensis bacterial community and the processes that drive its assembly remain unclear. Results In this study, based on Illumina HiSeq high-throughput sequencing, we explored the diversity, structure and composition of Ferula sinkiangensis rhizosphere bacterial communities at different slope positions (upper, middle and bottom) and soil depths (0-10 cm, 10-25 cm, 25-40 cm) and their correlation with soil physicochemical properties. Actinobacteria (22.7%), Proteobacteria (18.6%), Acidobacteria (14.0%), Gemmatimonadetes (10.1%), Cyanobacteria (7.9%), Bacteroidetes (6.9%), Planctomycetes (3.9%), Verrucomicrobia (3.5%), Firmicutes (3.4%) and Chloroflexi (3.2%) were the dominant bacterial phyla in Ferula sinkiangensis rhizosphere soil. Variance analysis showed that the diversity and abundance of rhizosphere bacterial community in Ferula sinkiangensis were significantly different at various slope positions and soil depths. Specifically, the diversity of bacterial community was significantly higher at the top than the bottom of the slope, and the diversity and richness of bacterial community were significantly greater in the 0-10cm than the 25-40cm soil layer. Linear discriminant effect size (LEfSe) analysis showed the specific phyla and genera of bacteria affected by slope position and soil depth. For example, Planctomycetes, Sphingomonas , Rubrobacter and Adhaeribacter by slope position and significant impact on soil depth. In addition, distance-based redundancy analysis (db-RDA) and variance analysis showed that soil physicochemical factors jointly explained 29.81% of variation in Ferula sinkiangensis rhizosphere bacterial community structure. There was a significant positive correlation between available phosphorus（AP）and the diversity of Ferula sinkiangensis rhizosphere bacterial community ( p < 0.01), whereas pH largely explained the variation of Ferula sinkiangensis rhizosphere bacterial community structure (5.58%, p < 0.01), followed by altitude (5.53%), total salt (TS, 5.21%) and total phosphorus (TP, 4.90%). Conclusion Our results revealed the heterogeneity and variation trends of Ferula sinkiangensis rhizosphere bacterial community diversity and abundance on a fine spatial scale (slope position and depth) and shed new light on the interaction mechanisms between Ferula sinkiangensis rhizosphere bacterial community and soil physicochemical properties.

Soil physicochemical properties and bacterial community composition jointly affect crop yield

2020 ◽  
Vol 112 (5) ◽  
pp. 4358-4372
Author(s):  
Meiqi Chen ◽  
Jisheng Xu ◽  
Zengqiang Li ◽  
Bingzi Zhao ◽  
Jiabao Zhang
Keyword(s):  
Bacterial Community ◽  
Physicochemical Properties ◽  
Community Composition ◽  
Crop Yield ◽  
Bacterial Community Composition ◽  
Soil Physicochemical Properties

scholarly journals Comparison of Bacterial Communities in Soil Samples with and without Tomato Bacterial Wilt Caused by Ralstonia solanacearum Species Complex

2020 ◽  
Author(s):  
Ying Zhang ◽  
Anna Hu ◽  
Jianuan Zhou ◽  
Wenfei Zhang ◽  
Peng Li
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Diversity Index ◽  
Chemical Properties ◽  
Interaction Network ◽  
Wilt Disease ◽  
Community Diversity ◽  
Bacterial Strains

Abstract BackgroundRalstonia solanacearum is one of the most notorious soil-born phytopathogen that causes a severe wilt disease with deadly effects on many economically important crops. The microbial community structure and interactions are commonly changed between bacterial wilt susceptible soil and healthy soil. Here, the bacterial community structure, correlation analysis with soil chemical properties, interaction network of healthy soil (HS, nearly no disease happened at recent three years) and diseased soil (DS, suffered heavy bacterial wilt disease) were analyzed.ResultsCompared to DS, a higher bacterial community diversity index was found in HS, and the relative abundance of main genera Bacillus, Gaiellales, Roseiflexus, Gemmatimonadaceae, Nocardioides and Anaerolineacear reached significant level. Redundancy analysis on genus level indicated that rapid available phosphate played key role on bacterial community distribution difference, and showed negative correlation with the other four chemical properties. Interaction network analysis further demonstrated that the higher genus community diversity and more extensive interactions were existed in HS network and formed stable network, and the genera Mycobacterium, Cyanobacteria and Rhodobiaceae should be the key components that sustain the network stably. Seven clusters of orthologous groups reached significant level difference between HS and DS. Moreover, 55 bacterial strains with distinct antagonistic activities to R. solancearum were isolated and identified. ConclusionsIn summary, our findings indicate that the bacterial diversity and interaction network changed between the HS and DS samples, which are also provide a good research basis for future biological control to the bacterial wilt.

scholarly journals Comparison of Bacterial Communities in Soil Samples with and without Tomato Bacterial Wilt Caused by Ralstonia solanacearum Species Complex

2020 ◽  
Author(s):  
Ying Zhang ◽  
Anna Hu ◽  
Jianuan Zhou ◽  
Wenfei Zhang ◽  
Peng Li
Keyword(s):  
Bacterial Community ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Diversity Index ◽  
Organic Matter Content ◽  
Interaction Network ◽  
Wilt Disease ◽  
Community Diversity ◽  
Bacterial Strains ◽  
Genus Level

Abstract Background: Ralstonia solanacearum is one of the most notorious soil-borne phytopathogens. It causes a severe wilt disease with deadly effects on many economically important crops. The microbita of disease-suppressive soils are thought that they can contribute to the disease resistance of crop plants, thus, to conduct the microbial community and their interaction characteristics between suppressive soil (SS) and conducive soil (CS) will help to understand resistance mechanism. Here, the bacterial community structure, correlation analysis with soil chemical properties, interaction network of SS (nearly no disease in three years), and CS (suffered heavy bacterial wilt disease) were analyzed. Results: Compared with CS, a higher bacterial community diversity index was found in SS , and the relative abundance of main genera Bacillus , Gaiellales , Roseiflexus , Gemmatimonadaceae , Nocardioides , and Anaerolineacear reached significant levels. Redundancy analysis at the genus level indicated that the available phosphate played a key role in the bacterial community distribution, and its role was negatively correlated with soil pH, organic matter content, alkali-hydrolyzable nitrogen, and available potassium contents. Interaction network analysis further demonstrated that greater diversity at the genus level existed in the SS network and formed a stable network. Additionally, the species of Mycobacterium , Cyanobacteria , and Rhodobiaceae are the key components that sustain the network stability. Seven clusters of orthologous groups exhibited significant differences between SS and CS. Moreover, 55 bacterial strains with distinct antagonistic activities to R. solancearum were isolated and identified. Conclusions: Our findings indicate that the bacterial diversity and interaction network differed between the CS and SS samples, providing a good foundation in the study of bacterial wilt.

scholarly journals Effects of Sphaeropsis Blight on Rhizosphere Soil Bacterial Community Structure and Soil Physicochemical Properties of Pinus sylvestris var. mongolica in Zhanggutai, China

Forests ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 954
Author(s):  
Saiyaremu Halifu ◽  
Xun Deng ◽  
Xiaoshuang Song ◽  
Yuning An ◽  
Ruiqing Song
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Pinus Sylvestris ◽  
Physicochemical Properties ◽  
Bacterial Community Structure ◽  
Rhizosphere Soil ◽  
Liaoning Province ◽  
Soil Physicochemical Properties ◽  
Α Diversity ◽  
Significant Difference

Pinus sylvestris var. mongolica is an important tree species for ecological construction and environmental restoration owing to its rapid growth rate and excellent stress resistance. Pinus sylvestris var. mongolica sphaeropsis blight is a widespread disease caused by Sphaeropsis sapinea. This study was focused on non-infected (CK) and infected (SS) Pinus sylvestris var. mongolica plants in Zhanggutai area, Liaoning Province, China. Illumina high-throughput sequencing based on the templates of sequencing-by-synthesis working with reversible terminators is a widely used approach. In the present study, systematic differences in relationships among rhizosphere soil physicochemical properties, bacterial community structure, diverse bacterial genera, and alpha diversity indices between the two categories were evaluated. The current findings are as follows: (1) Shannon’s index of SS soil was significantly higher than CK, and it was significantly lower in May than July and September (p < 0.05). (2) Non-metric multidimensional scaling (NMDS) showed a difference in bacterial community structure during May (spring), July (summer), and September. (3) At the phylum level, no significant difference was found in the bacterial genera between CK and SS soil for three seasons; however, at the genus level, there were about 19 different bacterial genera. The correlation studies between 19 different bacterial genera and environmental factors and α-diversity indicated that bacterial genera of non-infected and infected Pinus sylvestris var. mongolica were distributed differently. The bacterial genera with CK were positively correlated with soil physicochemical properties, while a negative correlation was found for SS. In conclusion, the differences in nutrient and microbial community structure in the rhizosphere soil of Pinus sylvestris var. mongolica are the main causes of shoot blight disease.

scholarly journals No tillage and residue mulching method on bacterial community diversity regulation in a black soil region of Northeastern China

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0256970
Author(s):  
Lijun Cai ◽  
Zhenhua Guo ◽  
Jingtao Zhang ◽  
Zhijia Gai ◽  
Jingqi Liu ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Physicochemical Properties ◽  
Black Soil ◽  
Sanjiang Plain ◽  
Northeastern China ◽  
Soil Bacterial Community ◽  
No Tillage ◽  
Soil Physicochemical Properties ◽  
The Sanjiang Plain ◽  
Soil Bacterial

Soil microorganisms are important components of agricultural ecosystems; they are important in agricultural soil nutrient cycle and are easily affected by soil tillage. The response of soil microbial community to tillage is very complex, and the effect of the no tillage and residue mulching method on soil microbial diversity remains unclear. In 2019, the soil was collected from an experimental field after 10 years of continuous cultivation in the black soil area of the Sanjiang Plain in Northeastern China. In this study, the diversity and composition of the soil bacterial community and their relationship with soil properties were explored via high-throughput sequencing under no tillage with four residue mulching treatments. No tillage with 60% residue mulching (NTR3) significantly increased the alpha diversity of the rhizosphere soil bacteria and changed the composition of the bacterial community—consistent with changes in soil physicochemical properties. Proteobacteria, Acidobacteria, and Actinobacteria were the dominant phyla in the sample soil. Soil physicochemical properties explained 80.6% of the changes in soil diversity and composition, of which soil organic carbon, soil pH, and soil temperature were the principal contributors. Our results suggest that no tillage and residue mulching is conducive to increasing soil organic carbon and soil nutrient content, which is a beneficial conservation tillage measure for black soil protection in Sanjiang Plain of Northeast China. The no tillage with residue mulching, especially 60% residue mulching, alters soil bacterial community and highlights the importance of soil physicochemical properties in shaping the diversity and composition of the soil bacterial community. Our findings contribute to a broad understanding of the effects of no tillage and residue mulching on bacterial community differences and provide a scientific basis for the optimization of no tillage measures and sustainable utilization of the black soil of the Sanjiang Plain in Northeastern China.

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