A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
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AbstractThe inability to over-express Aquaporin 6 (AQP6) in the plasma membrane of heterologous cells has hampered efforts to further characterize the function of this aquaglyceroporin membrane protein at atomic detail using crystallographic approaches. Using an Aquaporin 3-tGFP Reporter (AGR) system we have identified a region within loop C of AQP6 that is responsible for severely hampering plasma membrane expression. Serine substitution corroborated that amino acids present within AQP6194–213 of AQP6 loop C contribute to intracellular endoplasmic reticulum (ER) retention. This intracellular retention signal may preclude proper plasma membrane trafficking and severely curtail expression of AQP6 in heterologous expression systems.
2021 ◽
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2004 ◽
Vol 24
(11)
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pp. 5039-5049
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2009 ◽
Vol 296
(4)
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pp. C857-C867
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2009 ◽
Vol 53
(4)
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pp. 1305-1313
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2010 ◽
Vol 285
(37)
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pp. 28529-28539
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