scholarly journals Effect of Geobacillus toebii GT-02 addition on composition transformations and microbial community during thermophilic fermentation of bean dregs

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaojia Chen ◽  
Chengjian Wu ◽  
Xiang Li ◽  
Chenyang Wang ◽  
Qinyu Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Dry Matter ◽  
Nitrogen Loss ◽  
Organic Fertilizer ◽  
Promoting Effect ◽  
Dry Matter Loss ◽  
Thermophilic Fermentation ◽  
Rdna Sequencing ◽  
Microbial Number ◽  
Bean Dregs

AbstractBean dregs can be prepared into organic fertilizer by microbial fermentation. Geobacillus toebii GT-02, which has promoting effect on bean dregs fermentation, was isolated from horse dung and it grows within a range of 40–75 °C and pH 6.50–9.50. The effectiveness of GT-02 addition on composition transformations and the microbial community in bean dregs thermophilic fermentation at 70 °C for 5 days was investigated (T1). Fermentation of bean dregs without GT-02 served as control (CK). The results showed that T1 (the germination index (GI) = 95.06%) and CK (GI = 86.42%) reached maturity (defined by GI ≥ 85%) on day 3 and day 5, respectively. In addition, the total nitrogen loss of T1 (18.46%) on day 3 was lower than that in CK (24.12%). After thermophilic fermentation, the total organic carbon and dry matter loss of T1 (53.51% and 54.16%) was higher than that in CK (41.72% and 42.82%). The mean microbial number in T1 was 4.94 × 107 CFUs/g dry matter, which was 5.37 times higher than that in CK. 16S rDNA sequencing identified Bacillus, Geobacillus and Thermobacillus as dominant in CK, while Bacillus, Ammoniibacillus and Geobacillus were dominant in T1. A canonical correspondence analysis showed that Geobacillus and Ammoniibacillus were positively correlated with the GI. Thus, thermophilic fermentation with GT-02 can promote the maturity of bean dregs, which indicated the potential application value of GT-02 in thermophilic fermentation.

Effect of Geobacillus toebii addition on composition transformations and microbial community in thermophilic bean dreg composting

2021 ◽  
Author(s):  
Xiaojia Chen ◽  
Chengjian Wu ◽  
Chenyang Wang ◽  
Qinyu Li ◽  
Peng Zhou ◽  
...  
Keyword(s):  
Microbial Community ◽  
Dry Matter ◽  
Thermophilic Bacterium ◽  
Commercial Application ◽  
Dry Matter Loss ◽  
Rdna Sequencing ◽  
Microbial Number ◽  
Nitrogen Contents ◽  
Bean Dregs ◽  
Lower Carbon

Abstract A thermophilic bacterium, Geobacillus toebii GT-02, was successfully isolated from horse dung. The effectiveness of Geobacillus toebii GT-02 addition on composition transformations and the microbial community in bean dreg composting at 70°C for 5 days was investigated (T1), and the control consisted of bean dreg compost without GT-02 (CK). After 5 days, T1 presented higher total nitrogen contents (2.77%) and lower carbon-to-nitrogen ratios (14.4) than CK (2.34% and 16.9, respectively). The germination index (GI) of T1 was 135%, which was 56% higher than that of CK (86%). The dry matter loss in T1 was 54.16% (26.82% higher than the CK value (42.82%)). The mean microbial number in T1 was 4.94x107 CFUs/g dry matter, which was 5.37 times that in CK. 16S rDNA sequencing identified Bacillus, Geobacillus and Thermobacillus as dominant in CK, while Bacillus, Ammoniibacillus and Geobacillus were dominant in T1. A canonical correspondence analysis showed that Geobacillus and Ammoniibacillus were positively correlated with the GI. Thus, composting with GT-02 can promote the maturity of bean dregs, and GT-02 has potential commercial application in thermophilic composting.

scholarly journals Streptomyces sp. strain TOR3209: a rhizosphere bacterium promoting growth of tomato by affecting the rhizosphere microbial community

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Dong Hu ◽  
Shuhong Li ◽  
Ying Li ◽  
Jieli Peng ◽  
Xiaoyan Wei ◽  
...  
Keyword(s):  
Microbial Community ◽  
Plant Biomass ◽  
Organic Fertilizer ◽  
Growth Stages ◽  
Production Traits ◽  
Bacterial Strains ◽  
Promoting Effect ◽  
Tomato Production ◽  
Fruit Setting ◽  
Tomato Growth

AbstractAiming at revealing the possible mechanism of its growth promoting effect on tomato, the correlations among Streptomyces sp. TOR3209 inoculation, rhizobacteriome, and tomato growth/production traits were investigated in this study. By analyses of Illumina sequencing and plate coating, differences in rhizosphere microbial communities were found in different growth stages and distinct inoculation treatments. The plant biomass/fruit yields and relative abundances of families Flavobacteriaceae, Sphingobacteriaceae, Polyangiaceae and Enterobacteriaceae in treatments T (tomato inoculated with TOR3209) and TF (tomato inoculated with TOR3209 + organic fertilizer) were higher than that in the controls (CK and CK+ organic fertilizer), respectively. The analysis of Metastats and LEfSe revealed that the genera Flavobacterium and Sorangium in seedling stage, Klebsiella in flowering stage, Collimonas in early fruit setting stage, and genera Micrococcaceae, Pontibacte and Adhaeribacter in late fruit setting stage were the most representative rhizobacteria that positively responded to TOR3209 inoculation. By cultivation method, five bacterial strains positively correlated to TOR3209 inoculation were isolated from rhizosphere and root endosphere, which were identified as tomato growth promoters affiliated to Enterobacter sp., Arthrobacter sp., Bacillus subtilis, Rhizobium sp. and Bacillus velezensis. In pot experiment, TOR3209 and B. velezensis WSW007 showed joint promotion to tomato production, while the abundance of inoculated TOR3209 was dramatically decreased in rhizosphere along the growth of tomato. Conclusively, TOR3209 might promote the tomato production via changing of microbial community in rhizosphere. These findings provide a better understanding of the interactions among PGPR in plant promotion.

Machine learning algorithms to predict the dry matter loss of stored soybean grains ( Glycine max L.)

2021 ◽  
Author(s):  
Juliana Soares Zeymer ◽  
Felipe Guzzo ◽  
Marcos Eduardo Viana Araujo ◽  
Richard Stephen Gates ◽  
Paulo Cesar Corrêa ◽  
...  
Keyword(s):  
Machine Learning ◽  
Glycine Max ◽  
Dry Matter ◽  
Learning Algorithms ◽  
Machine Learning Algorithms ◽  
Dry Matter Loss ◽  
Glycine Max L

scholarly journals Microbiological Activity during Co-Composting of Food and Agricultural Waste for Soil Amendment

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 928
Author(s):  
Vladimir Mironov ◽  
Anna Vanteeva ◽  
Alexander Merkel
Keyword(s):  
Microbial Community ◽  
Agricultural Waste ◽  
Temperature Changes ◽  
Culture Independent ◽  
Mineralization Of Organic Matter ◽  
Microbial Number ◽  
Mature Compost ◽  
Stimulate Plant Growth ◽  
And Function ◽  
Favorable Conditions

This study aims to establish the relationship between ambient parameters and the diversity, composition, and function of microbial communities that predominate at each stage of the co-composting of food and agricultural waste. Culture-based and culture-independent methods were used to investigate the changes in the microbiota. The favorable conditions of high initial humidity and C/N ratio caused a decrease in the richness and biodiversity of the microbiota when such conditions existed. During the thermophilic stage, the total microbial number increased, and active mineralization of organic matter was carried out by members of the genera Bacillus, Caldibacillus, Aspergillus, and Penicillium. The fungal community was sensitive to drastic temperature changes. Byssochlamys dominated among fungi during the transition from the mesophilic to the thermophilic stage and during cooling. The biodiversity increased with time and was associated with the dynamics of germination and nitrification indices, so that the more diverse the microbial community, the higher the properties of compost that stimulate plant growth and development. The microbial community of the mature compost, together with mineral plant nutrients ready for consumption and humic compounds, make this compost a good soil additive.

scholarly journals BIOGAS PRODUCTION FROM AMARANTH BIOMASS

2013 ◽  
Vol 10 (2) ◽  
pp. 59-62
Author(s):  
Vladimír Sitkey ◽  
Ján Gaduš ◽  
Ľubomír Kliský ◽  
Alexander Dudák
Keyword(s):  
Dry Matter ◽  
Large Scale ◽  
Biogas Production ◽  
Organic Fertilizer ◽  
Matter Content ◽  
Raw Material ◽  
Dry Matter Content ◽  
Optimum Conditions ◽  
Average Yield ◽  
Amaranthus Spp

Abstract Energy variety of amaranth (Amaranthus spp.) was grown in large-scale trials in order to verify the capability of its cultivation and use as a renewable energy source in a biogas plant. The possibility of biogas production using anaerobic co-fermentation of manure and amaranth silage was verified in the experimental horizontal fermentor of 5 m3 volume, working at mesophilic conditions of 38-40 °C. The goal of the work was also to identify the optimum conditions for growth, harvesting and preservation of amaranth biomass, to optimize biogas production process, and to test the residual slurry from digestion process as a high quality organic fertilizer. The average yield of green amaranth biomass was 51.66 t.ha-1 with dry matter content of 37%. Based on the reached results it can be concluded that amaranth silage, solely or together with another organic materials of agricultural origin, is a suitable raw material for biogas production.

Losses from cattle faeces during chemical analysis

1974 ◽  
Vol 14 (71) ◽  
pp. 716 ◽  
Author(s):  
L Falvey ◽  
A Woolley
Keyword(s):  
Dry Matter ◽  
Nitrogen Loss ◽  
Routine Method ◽  
Drying Time ◽  
Sample Digestion ◽  
Faecal Material ◽  
Kjeldahl Method ◽  
Cattle Faeces ◽  
Nitrogen Contents

The effects of drying temperature and method of sample digestion on the determination of nitrogen and dry matter in the faeces of cattle grazing pastures of two different nitrogen contents were examined. Nitrogen loss varied from 8 to 20 per cent depending on the temperatures and associated length of drying time. The variation in the loss between samples from animals grazing the two pastures was small, tending to be higher for the samples taken from animals grazing a higher nitrogen pasture. Sample digestion by the H2SO4-H2O2 method was found to be as satisfactory as the micro-Kjeldahl method. Drying at 100�C for 24 hours gave the least loss of nitrogen. This procedure in conjunction with the H2SO4-H2O2 , digestion is proposed as a routine method for determination of nitrogen in faecal material.

scholarly journals Evaluating the effects of storage conditions on dry matter loss and nutritional quality of grain legume fodders in West Africa

2020 ◽  
Vol 262 ◽  
pp. 114419
Author(s):  
D.B. Akakpo ◽  
I.J.M de Boer ◽  
S. Adjei-Nsiah ◽  
A.J. Duncan ◽  
K.E. Giller ◽  
...  
Keyword(s):  
West Africa ◽  
Nutritional Quality ◽  
Dry Matter ◽  
Storage Conditions ◽  
Grain Legume ◽  
Dry Matter Loss

Carbon Dioxide Evolution from High-Moisture Shelled Corn Treated with Iprodione†

1995 ◽  
Vol 58 (6) ◽  
pp. 673-677 ◽  
Author(s):  
SLAVEN ALJINOVIC ◽  
CARL J. BERN ◽  
PRINCE N. DUGBA ◽  
MANJIT K. MISRA
Keyword(s):  
Carbon Dioxide ◽  
Untreated Control ◽  
Dry Matter ◽  
Storage Life ◽  
The Other ◽  
High Moisture ◽  
Carbon Dioxide Evolution ◽  
Dry Matter Loss

Carbon dioxide evolution was used to determine the storage life of 22.7% moisture shelled corn. Four iprodione fungicide treatments plus an untreated control were tested. The fungicide was tested on corn having three levels of mechanical kernel damage: 7% (hand shelled), 25% (combine harvested), and 16% (a blend of the other two damage levels). All iprodione treatments significantly increased storage life. Corn samples with higher levels of kernel damage took shorter times to reach the 0.5% dry-matter loss (DML) level. For combine-shelled corn, the fungicide increased storage life 17% at 15 mg/kg of corn and 46% at 20 mg/kg of corn.

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