scholarly journals A tube-source X-ray microtomography approach for quantitative 3D microscopy of optically challenging cell-cultured samples

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Ilmari Tamminen ◽  
Kalle Lehto ◽  
Markus Hannula ◽  
Miina Ojansivu ◽  
Laura Johansson ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Structure ◽  
Large Cell ◽  
Optical Methods ◽  
Cell Nuclei ◽  
Tissue Engineering Scaffolds ◽  
X Ray ◽  
Cell Structures ◽  
X Ray Imaging ◽  
Spatial Coverage

Abstract Development and study of cell-cultured constructs, such as tissue-engineering scaffolds or organ-on-a-chip platforms require a comprehensive, representative view on the cells inside the used materials. However, common characteristics of biomedical materials, for example, in porous, fibrous, rough-surfaced, and composite materials, can severely disturb low-energy imaging. In order to image and quantify cell structures in optically challenging samples, we combined labeling, 3D X-ray imaging, and in silico processing into a methodological pipeline. Cell-structure images were acquired by a tube-source X-ray microtomography device and compared to optical references for assessing the visual and quantitative accuracy. The spatial coverage of the X-ray imaging was demonstrated by investigating stem-cell nuclei inside clinically relevant-sized tissue-engineering scaffolds (5x13 mm) that were difficult to examine with the optical methods. Our results highlight the potential of the readily available X-ray microtomography devices that can be used to thoroughly study relative large cell-cultured samples with microscopic 3D accuracy.

scholarly journals X-ray imaging optimization of 3D tissue engineering scaffolds via combinatorial fabrication methods

Biomaterials ◽  
2008 ◽  
Vol 29 (12) ◽  
pp. 1901-1911 ◽  
Author(s):  
Yanyin Yang ◽  
Shauna M. Dorsey ◽  
Matthew L. Becker ◽  
Sheng Lin-Gibson ◽  
Gary E. Schumacher ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Tissue Engineering Scaffolds ◽  
X Ray ◽  
X Ray Imaging

Solvent Free Fabrication of Biodegradable Porous Polymers

2004 ◽  
Author(s):  
Xiaoxi Wang ◽  
Wei Li ◽  
Vipin Kumar
Keyword(s):  
Tissue Engineering ◽  
Polymer System ◽  
Solvent Free ◽  
Porous Polymers ◽  
Tissue Engineering Scaffolds ◽  
Co2 Gas ◽  
Biochemical Sensors ◽  
Foaming Process ◽  
Cell Structures ◽  
Chemical Blowing Agents

Biodegradable porous polymers with interconnected pores of sub-micrometers to a few hundred micrometers find many applications in emerging technology areas such as tissue engineering, controlled drug delivery, and biochemical sensors. However, most of the current fabrication processes involve organic solvents and chemical blowing agents that may cause environmental concerns and leave residues harmful to biological cells. This paper presents a solvent free fabrication approach for biodegradable porous polymers. Ultrasound cavitation is introduced after the solid state foaming process to produce open cell structures. The material used in this study is polylactic acid (PLA). It belongs to a family of biodegradable polymers that can be used for tissue engineering scaffolds. In order to identify suitable conditions to apply ultrasound, a saturation and foaming study is conducted for the PLA-CO2 gas polymer system. The effects of various process variables are discussed.

Investigation of particle-functionalized tissue engineering scaffolds using X-ray tomographic microscopy

2008 ◽  
Vol 100 (4) ◽  
pp. 820-829 ◽  
Author(s):  
J.V. Nygaard ◽  
M.Ø. Andersen ◽  
K.A. Howard ◽  
M. Foss ◽  
C. Bünger ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Tissue Engineering Scaffolds ◽  
X Ray

Influence of Particles on Cell Structure of Al-Mg Alloys Foamed by Melt Foaming Method

2007 ◽  
Vol 544-545 ◽  
pp. 363-366
Author(s):  
Soo Han Park ◽  
Hwan Goo Seong ◽  
Yeong Hwan Song ◽  
Chang Hwan Seo ◽  
Zulkifli ◽  
...  
Keyword(s):  
Cell Structure ◽  
Surface Structures ◽  
Mg Alloy ◽  
X Ray ◽  
Cell Structures ◽  
Negative Effect ◽  
Oxide Particles ◽  
Al Mg Alloys ◽  
Scanning Electron ◽  
Microstructural Examination

Al-Mg alloy foams containing different Mg contents were synthesized via a conventional melt foaming method. The surface structures of pores formed in resultant foamed alloys was characterized by scanning electron microscopy and x-ray diffractometer. It was found that the pore structures were deteriorated with increasing Mg contents, while the percent porosities did not vary with increasing Mg contents, about 90% and 3~5 mm in pore size. The detailed microstructural examination conducted on increasing Mg containing alloy foams revealed presence of various oxide particles on the surface of individual cells, including MgAl2O4 particles in a form of fine spinel; its small amount for Al-1wt%Mg alloy foam but relative high amount of Al-4wt%Mg one. This suggested the negative effect of MgAl2O4 on the stable pore and thus cell structures in corresponding alloy foams. The possible mechanism associated with MgAl2O4 formation was discussed in the present study.

Low-dose phase-based X-ray imaging techniques for in situ soft tissue engineering assessments

Biomaterials ◽  
2016 ◽  
Vol 82 ◽  
pp. 151-167 ◽  
Author(s):  
Zohreh Izadifar ◽  
Ali Honaramooz ◽  
Sheldon Wiebe ◽  
George Belev ◽  
Xiongbiao Chen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Low Dose ◽  
Imaging Techniques ◽  
X Ray ◽  
X Ray Imaging ◽  
Soft Tissue Engineering

scholarly journals Data of low-dose phase-based X-ray imaging for in situ soft tissue engineering assessments

Data in Brief ◽  
2016 ◽  
Vol 6 ◽  
pp. 644-651 ◽  
Author(s):  
Zohreh Izadifar ◽  
Ali Honaramooz ◽  
Sheldon Wiebe ◽  
George Belev ◽  
Xiongbiao Chen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Low Dose ◽  
X Ray ◽  
X Ray Imaging ◽  
Soft Tissue Engineering

scholarly journals Porosity Pattern of 3D Chitosan/Bioactive Glass Tissue Engineering Scaffolds Prepared for Bone Regeneration

2021 ◽  
Vol 15 (1) ◽  
pp. 41-56
Author(s):  
Hoda G.H. Hammad ◽  
Miral Nagy F. Salama
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Regeneration ◽  
Phase Identification ◽  
Tissue Engineering Scaffolds ◽  
Composite Scaffolds ◽  
X Ray ◽  
Pore Sizes ◽  
Porous Chitosan ◽  
Scanning Electron

Aim: The study was conducted to investigate the obtained external and internal porosity and the pore-interconnectivity of specific fabricated bioactive composite tissue engineering scaffolds for bone regeneration in dental applications. Materials and Methods: In this study, the bioactive glass [M] was elaborated as a quaternary system to be incorporated into the chitosan [C] scaffold preparation on a magnetic stirrer to provide bioactivity and better strength properties for the attempted composite scaffolds [C/ M] of variable compositions. The homogenous chitosan/bioactive glass mix was poured into tailor-made cylindrical molds [10cm×10cm]; a freeze-dryer program was used for the creation of uniform and interconnected macropores for all prepared chitosan-based scaffolds. The morphology of fabricated chitosan [C] and chitosan-bioactive glass [C/ M] composite scaffolds was studied by a scanning electron microscope [SEM] and a mercury porosimeter. In addition, the in-vitro biodegradation rate of all elaborated scaffolds was reported after immersing the prepared scaffolds in a simulated body fluid [SBF] solution. Furthermore, for every prepared scaffold composition, characterization was performed for phase identification, microstructure, porosity, bioactivity, and mechanical properties using an X-ray diffraction analysis [XRD], an X-ray Fourier transfer infrared spectroscopy [FTIR], a mercury porosimetry, a scanning electron microscopy [SEM] coupled to an energy-dispersive X-ray spectrometry [EDS] and a universal testing machine, respectively. Results: All the prepared porous chitosan-based composite materials showed pore sizes suitable for osteoblasts seeding, with relatively larger pore sizes for the C scaffolds. Conclusion: The smart blending of the prepared bioactive glass [M] with the chitosan matrix offered some advantages, such as the formation of an apatite layer for cell adhesion upon the scaffold surfaces, the reasonable decrease in scaffold pore size, and the relative increase in compressive strength that were enhanced by the incorporation of [M]. Therefore, the morphology, microstructure, and mechanical behavior of the elaborated stress loaded biocomposite tissue engineering scaffolds seem highly dependent on their critical contented bioactive glass.

Study on Tissue Engineering Scaffolds of Silk Fibroin-Chitosan/ Nano-Hydroxyapatite Composite

2007 ◽  
Vol 330-332 ◽  
pp. 971-975 ◽  
Author(s):  
Guang Wu Wen ◽  
Jing Wang ◽  
Mu Qin Li ◽  
Xiang Cai Meng
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Porous Scaffolds ◽  
High Porosity ◽  
Tissue Engineering Scaffolds ◽  
Component Structure ◽  
X Ray ◽  
Hydroxyapatite Composite ◽  
Ray Diffusion ◽  
Interconnected Pores

The porous scaffolds of silk fibroin-chitosan /nano-hydroxyapatite (SF-CS / n-HA) were fabricated through the freeze- drying technique. Component, structure and morphology of scaffolds were studied by infrared (IR), X-ray diffusion (XRD) and scanning electron microscope (SEM), and the mechanical properties of the scaffolds were measured. The simulated body fluid (SBF) experiments were conducted to assess the bioactivity of the scaffolds. Results indicate that chemical binding is formed between HA and organics, the macropore diameter of the scaffolds varies from 150 to 400μm. The porous scaffolds with interconnected pores possess a high porosity of 78%-91% and compressive strength of 0.26 -1.96MPa, which can be controlled by adjusting the concentration of organic phases and prefreezing temperature. In the SBF tests, a layer of randomly oriented bone-like apatite crystals formed on the scaffold surface, which suggested that the composite material had good bioactivity. Studies suggest the feasibility of using SF-CS /n-HA composite scaffolds for bone tissue engineering.

Preparation of oriented bacterial cellulose nanofibers by flowing medium-assisted biosynthesis and influence of flowing velocity

2018 ◽  
Vol 38 (3) ◽  
pp. 299-305 ◽  
Author(s):  
Honglin Luo ◽  
Wei Li ◽  
Zhiwei Yang ◽  
Haiyong Ao ◽  
Guangyao Xiong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bacterial Cellulose ◽  
Culture Medium ◽  
Cellulose Nanofibers ◽  
X Ray Diffraction ◽  
Tissue Engineering Scaffolds ◽  
X Ray ◽  
Biomimetic Scaffolds ◽  
Nanofiber Alignment ◽  
Thermal Stability Of

AbstractNanofiber alignment in tissue engineering scaffolds is a crucial factor controlling the cell behavior. In this work, we report a facile approach to obtain aligned nanofibers of bacterial cellulose (BC) by forcing the culture medium of bacteria to flow along a fixed direction. The emphasis of this work was placed on the effect of flowing velocity on the alignment of the as-prepared oriented BC (OBC). X-ray diffraction (XRD) and Fourier transform infrared (FTIR) analyses indicated that the velocity affected the crystallinity and thermal stability of BC while the chemical structure did not change with the velocity. The controllable alignment of BC nanofibers makes them a promising material for the construction of biomimetic scaffolds for tissue engineering and regenerative medicine.

Defects of Platelet Function Recognized by X-Ray Imaging and Scanning Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 610-613
Author(s):  
M.G. Baldini ◽  
S. Morinaga ◽  
D. Minasian ◽  
R. Feder ◽  
D. Sayre ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Biology ◽  
Imaging Technique ◽  
Human Platelets ◽  
X Rays ◽  
X Ray ◽  
X Ray Imaging ◽  
Complex Phenomena ◽  
Scanning Electron

Contact X-ray imaging is presently developing as an important imaging technique in cell biology. Our recent studies on human platelets have demonstrated that the cytoskeleton of these cells contains photondense structures which can preferentially be imaged by soft X-ray imaging. Our present research has dealt with platelet activation, i.e., the complex phenomena which precede platelet appregation and are associated with profound changes in platelet cytoskeleton. Human platelets suspended in plasma were used. Whole cell mounts were fixed and dehydrated, then exposed to a stationary source of soft X-rays as previously described. Developed replicas and respective grids were studied by scanning electron microscopy (SEM).

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