scholarly journals E-cadherin–downregulation and RECK-upregulation are coupled in the non-malignant epithelial cell line MCF10A but not in multiple carcinoma-derived cell lines

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Kanako Yuki ◽  
Yoko Yoshida ◽  
Ryosaku Inagaki ◽  
Hiroshi Hiai ◽  
Makoto Noda
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Epithelial Cell Line ◽  
Multiple Carcinoma ◽  
E Cadherin

scholarly journals Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.

1988 ◽  
Vol 106 (3) ◽  
pp. 761-771 ◽  
Author(s):  
P Boukamp ◽  
R T Petrussevska ◽  
D Breitkreutz ◽  
J Hornung ◽  
A Markham ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Human Skin ◽  
Cell Lines ◽  
Hacat Cell ◽  
Epithelial Cell Line ◽  
Spontaneous Transformation ◽  
Chromosomal Alterations ◽  
Hacat Cell Line

In contrast to mouse epidermal cells, human skin keratinocytes are rather resistant to transformation in vitro. Immortalization has been achieved by SV40 but has resulted in cell lines with altered differentiation. We have established a spontaneously transformed human epithelial cell line from adult skin, which maintains full epidermal differentiation capacity. This HaCaT cell line is obviously immortal (greater than 140 passages), has a transformed phenotype in vitro (clonogenic on plastic and in agar) but remains nontumorigenic. Despite the altered and unlimited growth potential, HaCaT cells, similar to normal keratinocytes, reform an orderly structured and differentiated epidermal tissue when transplanted onto nude mice. Differentiation-specific keratins (Nos. 1 and 10) and other markers (involucrin and filaggrin) are expressed and regularly located. Thus, HaCaT is the first permanent epithelial cell line from adult human skin that exhibits normal differentiation and provides a promising tool for studying regulation of keratinization in human cells. On karyotyping this line is aneuploid (initially hypodiploid) with unique stable marker chromosomes indicating monoclonal origin. The identity of the HaCaT line with the tissue of origin was proven by DNA fingerprinting using hypervariable minisatellite probes. This is the first demonstration that the DNA fingerprint pattern is unaffected by long-term cultivation, transformation, and multiple chromosomal alterations, thereby offering a unique possibility for unequivocal identification of human cell lines. The characteristics of the HaCaT cell line clearly document that spontaneous transformation of human adult keratinocytes can occur in vitro and is associated with sequential chromosomal alterations, though not obligatorily linked to major defects in differentiation.

scholarly journals Different Roles of Estrogen Receptors α and β in the Regulation of E-Cadherin Protein Levels in a Mouse Mammary Epithelial Cell Line

2008 ◽  
Vol 68 (21) ◽  
pp. 8695-8704 ◽  
Author(s):  
Luisa A. Helguero ◽  
Karolina Lindberg ◽  
Cissi Gardmo ◽  
Thomas Schwend ◽  
Jan-Åke Gustafsson ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Estrogen Receptors ◽  
Cell Line ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Mammary Epithelial Cell Line ◽  
Protein Levels ◽  
Mouse Mammary Epithelial Cell ◽  
E Cadherin

scholarly journals Sulphamoylated estradiol analogue induces antiproliferative activity and apoptosis in breast cell lines

2012 ◽  
Vol 17 (4) ◽  
Author(s):  
Michelle Visagie ◽  
Thandi Mqoco ◽  
Anna Joubert
Keyword(s):  
Estrogen Receptor ◽  
Epithelial Cell ◽  
Cell Line ◽  
Cell Density ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Breast Adenocarcinoma ◽  
Epithelial Cell Line ◽  
Antimitotic Activity

AbstractResearch into potential anticancer agents has shown that 2-methoxyestradiol exerts antiproliferative activity in vitro and in vivo in an estrogen receptor-independent manner. Due to its limited biological accessibility and rapid metabolic degradation, several new analogues have been developed in recent years. This study investigated the in vitro effects of a novel in silicodesigned compound (C16) in an estrogen receptor-positive breast adenocarcinoma epithelial cell line (MCF-7), an estrogen receptor-negative breast adenocarcinoma epithelial cell line (MDA-MB-231) and a nontumorigenic breast cell line (MCF-12A). Light microscopy revealed decreased cell density, cells blocked in metaphase and the presence of apoptotic characteristics in all three cell lines after exposure to C16 for 24 h. Polarizationoptical transmitted light differential interference contrast revealed the presence of several rounded cells and decreased cell density. The xCELLigence real-time label-independent approach revealed that C16 exerted antiproliferative activity. Significant inhibition of cell growth was demonstrated after 24 h of exposure to 0.2 μM C16 in all three cell lines. However, the non-tumorigenic MCF-12A cell line recovered extremely well after 48 h when compared to the tumorigenic cell lines. This indicates that C16 acts as an antiproliferative agent, possesses antimitotic activity and induces apoptosis in vitro. These features warrant further investigation.

Interactions of Modern Aesthetic Materials for Prosthetic Restorations with Oral Mucosa A pilot study

2018 ◽  
Vol 69 (2) ◽  
pp. 386-390
Author(s):  
Bogdan Calenic ◽  
Mihaela Pantea ◽  
Ana Maria Cristina Tancu ◽  
Paula Perlea ◽  
Maria Greabu ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Polymethyl Methacrylate ◽  
Oral Mucosa ◽  
Precious Metal ◽  
Metal Alloy ◽  
Health Science ◽  
Epithelial Cell Line ◽  
Gingival Epithelial Cell ◽  
E Cadherin

The specific objective of the present study is to assess the interactions between cells from a human gingival epithelial cell line and various aesthetic materials used in modern prosthetic dentistry. For this study six types of dental materials were selected: Cr-Co non-precious metal alloy, ceramics applied on Cr-Co non-precious metal alloy, zirconia, ceramics applied on zirconia, polymethyl methacrylate and pressed ceramics/lithium disilicate. Cells from a human gingival epithelial cell line, Ca9-22 (Health Science Research Resources Bank), were cultured on the chosen surfaces for 3, 5 and 7 days. Cellular proliferation, cell attachment (using Multiplex Arrays Technology) and cytotoxicity (MTT- Assay) were evaluated at distinct predetermined intervals. Measurements performed at each distinct predetermined interval showed no significant difference for cell proliferation and cytotoxicity between the selected surfaces, however the highest levels were registered for the polymethyl methacrylate surface. Different attachment patterns were observed for epithelial cells attached on substrates, such as significantly different levels of adherence of E-Cadherin and N-Cadherin molecules; E-Cadherin adhesion levels indicate that pressed ceramics may be the dental material which, compared to the selected materials, influences the least the homeostasis of oral mucosa.

scholarly journals Staphylococcus aureus Escapes More Efficiently from the Phagosome of a Cystic Fibrosis Bronchial Epithelial Cell Line than from Its Normal Counterpart

2006 ◽  
Vol 74 (5) ◽  
pp. 2568-2577 ◽  
Author(s):  
Todd M. Jarry ◽  
Ambrose L. Cheung
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Epithelial Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Microscopic Analysis ◽  
Bronchial Epithelial Cell ◽  
Epithelial Cell Line ◽  
Similar Degree ◽  
Electron Microscopic

ABSTRACTStaphylococcus aureusis frequently the initial bacterium isolated from young cystic fibrosis (CF) patients, and yet its role in CF disease progression has not been determined. Recent data from our lab demonstrates thatS. aureuscan invade and replicate within the CF tracheal epithelial cell line (CFT-1). Here we describe the finding that the fate of internalizedS. aureusin CFT-1 cells differs from its complemented counterpart (LCFSN).S. aureusstrain RN6390 was able to replicate within the mutant CFT-1 cells after invasion but not in the complemented LCFSN cells. At 1 h postinvasion,S. aureuscontaining vesicles within both cell lines acquired vacuolar-ATPase, lysosomal markers LAMP 1 and 2, and the lysomotrophic dye LysoTracker to a similar degree. However, at 4 h postinvasion, the percentage ofS. aureuswithin CFT-1 cells associated with these markers decreased significantly compared to LCFSN, where the association approached 100%. Transmission electron microscopic analysis revealed that the majority of bacteria within CFT-1 cells were free in the cytosol at 4 h after invasion, whereas mostS. aureusbacteria internalized by LCFSN cells remained within vesicles. These results demonstrate a fundamental difference in the fate of liveS. aureusafter invasion of CFT-1 versus LCFSN cell lines and may explain the propensity ofS. aureusto cause chronic lung infection in CF patients.

scholarly journals A Possible Role of FZD10 Delivering Exosomes Derived from Colon Cancers Cell Lines in Inducing Activation of Epithelial–Mesenchymal Transition in Normal Colon Epithelial Cell Line

2020 ◽  
Vol 21 (18) ◽  
pp. 6705 ◽  
Author(s):  
Maria Principia Scavo ◽  
Federica Rizzi ◽  
Nicoletta Depalo ◽  
Elisabetta Fanizza ◽  
Chiara Ingrosso ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Extracellular Vesicles ◽  
Epithelial Mesenchymal Transition ◽  
In Vitro Study ◽  
Cell Interaction ◽  
Epithelial Cell Line ◽  
Mesenchymal Transition

Exosomes belong to the family of extracellular vesicles released by every type of cell both in normal and pathological conditions. Growing interest in studies indicates that extracellular vesicles, in particular, the fraction named exosomes containing lipids, proteins and nucleic acid, represent an efficient way to transfer functional cargoes between cells, thus combining all the other cell–cell interaction mechanisms known so far. Only a few decades ago, the involvement of exosomes in the carcinogenesis in different tissues was discovered, and very recently it was also observed how they carry and modulate the presence of Wnt pathway proteins, involved in the carcinogenesis of gastrointestinal tissues, such as Frizzled 10 protein (FZD10), a membrane receptor for Wnt. Here, we report the in vitro study on the capability of tumor-derived exosomes to induce neoplastic features in normal cells. Exosomes derived from two different colon cancer cell lines, namely the non-metastatic CaCo-2 and the metastatic SW620, were found to deliver, in both cases, FZD10, thus demonstrating the ability to reprogram normal colonic epithelial cell line (HCEC-1CT). Indeed, the acquisition of specific mesenchymal characteristics, such as migration capability and expression of FZD10 and markers of mesenchymal cells, was observed. The exosomes derived from the metastatic cell line, characterized by a level of FZD10 higher than the exosomes extracted from the non-metastatic cells, were also more efficient in stimulating EMT activation. The overall results suggest that FZD10, delivered by circulating tumor-derived exosomes, can play a relevant role in promoting the CRC carcinogenesis and propagation.

Effects of a prostaglandin F2alpha derivative glaucoma drug on EGF expression and E-cadherin expression in a corneal epithelial cell line

2020 ◽  
Vol 39 (2) ◽  
pp. 75-82
Author(s):  
Yukihisa Takada ◽  
Osamu Yamanaka ◽  
Yuka Okada ◽  
Takayoshi Sumioka ◽  
Peter S. Reinach ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Corneal Epithelial Cell ◽  
Epithelial Cell Line ◽  
Prostaglandin F2alpha ◽  
Corneal Epithelial ◽  
E Cadherin

scholarly journals Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.

1996 ◽  
Vol 135 (6) ◽  
pp. 1643-1654 ◽  
Author(s):  
S Islam ◽  
T E Carey ◽  
G T Wolf ◽  
M J Wheelock ◽  
K R Johnson
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Cell Line ◽  
Squamous Cell ◽  
Epithelial Cell Line ◽  
Squamous Epithelial Cell ◽  
Squamous Carcinoma Cells ◽  
E Cadherin ◽  
Cell Cell

E-cadherin is a transmembrane glycoprotein that mediates calcium-dependent, homotypic cell-cell adhesion and plays an important role in maintaining the normal phenotype of epithelial cells. Disruption of E-cadherin activity in epithelial cells correlates with formation of metastatic tumors. Decreased adhesive function may be implemented in a number of ways including: (a) decreased expression of E-cadherin; (b) mutations in the gene encoding E-cadherin; or (c) mutations in the genes that encode the catenins, proteins that link the cadherins to the cytoskeleton and are essential for cadherin mediated cell-cell adhesion. In this study, we explored the possibility that inappropriate expression of a nonepithelial cadherin by an epithelial cell might also result in disruption of cell-cell adhesion. We showed that a squamous cell carcinoma-derived cell line expressed N-cadherin and displayed a scattered fibroblastic phenotype along with decreased expression of E- and P-cadherin. Transfection of this cell line with antisense N-cadherin resulted in reversion to a normal-appearing squamous epithelial cell with increased E- and P-cadherin expression. In addition, transfection of a normal-appearing squamous epithelial cell line with N-cadherin resulted in downregulation of both E- and P-cadherin and a scattered fibroblastic phenotype. In all cases, the levels of expression of N-cadherin and E-cadherin were inversely related to one another. In addition, we showed that some squamous cell carcinomas expressed N-cadherin in situ and those tumors expressing N-cadherin were invasive. These studies led us to propose a novel mechanism for tumorigenesis in squamous epithelial cells; i.e., inadvertent expression of a nonepithelial cadherin.

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