scholarly journals Effects of Nanotexture on Electrical Profiling of Single Tumor Cell and Detection of Cancer from Blood in Microfluidic Channels

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Muhymin Islam ◽  
Mohammad Motasim Bellah ◽  
Adeel Sajid ◽  
Mohammad Raziul Hasan ◽  
Young-tae Kim ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Microfluidic Channels ◽  
Single Tumor Cell ◽  
Single Tumor

scholarly journals Probing single-tumor cell interactions with different-age type I collagen networks by synchrotron-based Fourier transform infrared microspectroscopy

2014 ◽  
Vol 19 (11) ◽  
pp. 111612 ◽  
Author(s):  
Marie Guilbert ◽  
Christophe Eklouh-Molinier ◽  
Katia Wehbe ◽  
Josep Sulé-Suso ◽  
Ying Yang ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Tumor Cell ◽  
Type I Collagen ◽  
Fourier Transform Infrared ◽  
Cell Interactions ◽  
Type I ◽  
Infrared Microspectroscopy ◽  
Fourier Transform Infrared Microspectroscopy ◽  
Single Tumor Cell ◽  
Single Tumor

scholarly journals Real-time live-cell analysis system for screening single tumor cell clones and analyzing their colony-forming ability

2017 ◽  
Vol 25 (10) ◽  
pp. 881
Author(s):  
Chang-Zheng Liu ◽  
Xiao-Lei Jiao ◽  
Dun-Qin Gao ◽  
Long-Bin Xing ◽  
Hui Liu ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Real Time ◽  
Live Cell ◽  
Cell Analysis ◽  
Cell Clones ◽  
Analysis System ◽  
Single Tumor Cell ◽  
Single Tumor ◽  
Live Cell Analysis

A quantitative observation and imaging of single tumor cell migration and deformation using a multi-gap microfluidic device representing the blood vessel

2006 ◽  
Vol 72 (3) ◽  
pp. 153-160 ◽  
Author(s):  
K.C. Chaw ◽  
M. Manimaran ◽  
Francis E.H. Tay ◽  
S. Swaminathan
Keyword(s):  
Cell Migration ◽  
Blood Vessel ◽  
Tumor Cell ◽  
Microfluidic Device ◽  
Tumor Cell Migration ◽  
Single Tumor Cell ◽  
Single Tumor

The IMiDs® Immunomodulatory Drugs Revlimid® (Lenalidomide) and CC-4047 Induce Growth Arrest and Apoptosis in NHL Tumor Cells In Vitro.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 2388-2388 ◽  
Author(s):  
Laura G. Corral ◽  
Dan Zhu ◽  
Yuedi Wang ◽  
Bernd Stein
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Growth Arrest ◽  
Additive Effect ◽  
Immunomodulatory Drugs ◽  
Raji Cells ◽  
Pre Treatment ◽  
Single Tumor Cell ◽  
Single Tumor

Abstract IMiDs® immunomodulatory drugs are thalidomide analogues that have been developed for improved anti-cancer and anti-inflammatory properties and decreased side effects. Many IMiDs® immunomodulatory drugs have been shown to have activities in hematologic cancers and solid malignancies, as well as having profound effects on the bone marrow microenvironment. Specifically in NHL, it was shown that addition of Revlimid® or CC-4047 to Rituxan enhances anti-tumor activity in a SCID mouse lymphoma model. Here we tested the direct effects of Revlimid® and CC-4047 on NHL tumor cells by treating Raji cells with each drug alone or with each drug in combination with anti-CD20 antibodies B1 and Rituxan. CC-4047 alone caused up to 40% inhibition of proliferation at 10 μM in Raji cells, which corresponded to G1 arrest. In combination with B1, CC-4047 showed a small additive effect at 10 μM while Revlimid® effects were minimal up to 10 μM. In combination with Rituxan, CC-4047 showed a slight additive effect at 10 μM and Revlimid® at 50 μM. We have also developed a co-culture assay of PBMC and NHL tumor cells as an in vitro model of tumor-host immune system interaction, to further explore the anti-tumor potential of the drugs in NHL. This assay is non-radioactive and flow cytometry based. In this co-culture system using Raji and PBMC, we have shown that pre-treatment of PBMC with Revlimid® or CC-4047 can enhance the PBMC activity in inducing Raji cell apoptosis in a dose dependent manner. In addition, our data indicate that pre-treatment of Raji cells with Rituxan can further enhance the apoptosis induced by PBMC pre-treated with Revlimid® or CC-4047. Since minimal additive effect between each drug and Rituxan was observed in the Raji single tumor cell model, these studies suggest that the co-culture system is a more appropriate cellular model to assess the anti-tumor activities of certain IMiDs® immunomodulatory drugs. This system can reveal the effects of certain IMiD® immunomodulatory drugs not observable with single tumor cell proliferation models. In summary, our data clearly demonstrate that Revlimid® and CC-4047 directly induce NHL tumor cell growth arrest and effectively enhance tumor cell apoptosis induced by PBMC. These results support clinical evaluation of Revlimid® and certain IMiDs® immunomodulatory drugs in relapsed B-cell NHL in combination with Rituxan.

Electrochemiluminescence Biosensor for Nucleolin Imaging in a Single Tumor Cell Combined with Synergetic Therapy of Tumor

ACS Sensors ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 1216-1222 ◽  
Author(s):  
Wanxia Gao ◽  
Yong Liu ◽  
Huairong Zhang ◽  
Zonghua Wang
Keyword(s):  
Tumor Cell ◽  
Single Tumor Cell ◽  
Single Tumor

Prognostic impact of isolated tumor cells in breast cancer axillary nodes: single tumor cell(s) versus tumor cell cluster(s) and microanatomic location

2011 ◽  
Vol 131 (2) ◽  
pp. 645-651 ◽  
Author(s):  
Johanna H. Vestjens ◽  
Maaike de Boer ◽  
Paul J. van Diest ◽  
Carolien H. van Deurzen ◽  
Jos A. van Dijck ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Cell ◽  
Tumor Cells ◽  
Cell Cluster ◽  
Prognostic Impact ◽  
Isolated Tumor Cells ◽  
Axillary Nodes ◽  
Single Tumor Cell ◽  
Single Tumor

scholarly journals Detection of single tumor cell resistance with aptamer biochip

2012 ◽  
Vol 4 (5) ◽  
pp. 935-940 ◽  
Author(s):  
LIXUE WANG ◽  
QIN ZHENG ◽  
QUAN’AN ZHANG ◽  
HANFENG XU ◽  
JINLONG TONG ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Resistance ◽  
Single Tumor Cell ◽  
Single Tumor ◽  
Tumor Cell Resistance

scholarly journals Raman profile alterations of irradiated human nasopharyngeal cancer cells detected with laser tweezer Raman spectroscopy

RSC Advances ◽  
2020 ◽  
Vol 10 (24) ◽  
pp. 14368-14373 ◽  
Author(s):  
Sufang Qiu ◽  
Youliang Weng ◽  
Ying Li ◽  
Yang Chen ◽  
Yuhui Pan ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Statistical Analysis ◽  
Tumor Cell ◽  
Cancer Cells ◽  
Multivariate Statistical Analysis ◽  
Nasopharyngeal Cancer ◽  
Multivariate Statistical ◽  
Laser Tweezer ◽  
Single Tumor Cell ◽  
Single Tumor

Laser tweezer Raman spectroscopy combined with multivariate statistical analysis was used for evaluating the radiotherapeutic effect on a single tumor cell.

scholarly journals The Architecture of Co-Culture Spheroids Regulates Tumor Invasion within a 3D Extracellular Matrix

2020 ◽  
Vol 15 (03) ◽  
pp. 131-141
Author(s):  
Yu Ling Huang ◽  
Carina Shiau ◽  
Cindy Wu ◽  
Jeffrey E. Segall ◽  
Mingming Wu
Keyword(s):  
Extracellular Matrix ◽  
Tumor Microenvironment ◽  
Tumor Cell ◽  
Tumor Invasion ◽  
Cancer Metastasis ◽  
Tumor Spheroid ◽  
Micrometer Scale ◽  
Single Tumor Cell ◽  
Single Tumor ◽  
Invasion Assays

Tumor invasion, the process by which tumor cells break away from their primary tumor and gain access to vascular systems, is an important step in cancer metastasis. Most current 3D tumor invasion assays consisted of a single tumor cell embedded within an extracellular matrix (ECM). These assays taught us much of what we know today on how key biophysical (e.g., ECM stiffness) and biochemical (e.g., cytokine gradients) parameters within the tumor microenvironment guided and regulated tumor invasion. One limitation of the single tumor cell invasion assays was that it did not account for cell–cell adhesion within the tumor. In this paper, we developed a micrometer scale 3D co-culture spheroid invasion assay that recapitulated physiologically realistic tumor microenvironment and was compatible with microscopic imaging. Micrometer scale co-culture spheroids (1:1 ratio of metastatic breast cancer MDA-MB-231 and non-tumorigenic epithelial MCF-10A cells) were made using an array of microwells, and then were embedded within a collagen matrix in a microfluidic platform. Real time imaging of tumor spheroid invasion revealed that the spatial distribution of the two cell types within the tumor spheroid critically regulated tumor invasion. This work linked tumor architecture with tumor invasion and highlighted the importance of the biophysical cues within the bulk of the tumor in tumor invasion.

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