Efficient radical scavenging ability of artepillin C, a major component of Brazilian propolis, and the mechanism

2003 ◽  
Vol 1 (9) ◽  
pp. 1452-1454 ◽  
Author(s):  
Ikuo Nakanishi ◽  
Yoshihiro Uto ◽  
Kei Ohkubo ◽  
Kentaro Miyazaki ◽  
Haruko Yakumaru ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Artepillin C ◽  
Brazilian Propolis ◽  
Radical Scavenging Ability

scholarly journals Inhibition of Inflammatory Response by Artepillin C in Activated RAW264.7 Macrophages

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Ewelina Szliszka ◽  
Anna Mertas ◽  
Zenon P. Czuba ◽  
Wojciech Król
Keyword(s):  
Cell Viability ◽  
Radical Scavenging ◽  
Gm Csf ◽  
Raw264.7 Macrophages ◽  
Griess Reaction ◽  
Artepillin C ◽  
Anti Inflammatory ◽  
Radical Scavenging Ability ◽  
Brazilian Green Propolis ◽  
Inflammatory Effect

Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) is the main bioactive component of Brazilian green propolis. The aim of this study was to investigate the anti-inflammatory effect of artepillin C on LPS + IFN-γ- or PMA-stimulated RAW264.7 macrophages. The cell viability was evaluated by MTT and LDH assays. The radical scavenging ability was determined using DPPH•and ABTS•+. ROS and RNS generation was analyzed by chemiluminescence. NO concentration was detected by the Griess reaction. The release of various cytokines by activated RAW264.7 cells was measured in the culture supernatants using a multiplex bead array system based on xMAP technology. NF-κB activity was confirmed by the ELISA-based TransAM NF-κB kit. At the tested concentrations, the compound did not decrease the cell viability and did not cause the cytotoxicity. Artepillin C exerted strong antioxidant activity, significantly inhibited the production of ROS, RNS, NO, and cytokine IL-1β, IL-3, IL-4, IL-5, IL-9, IL-12p40, IL-13, IL-17, TNF-α, G-CSF, GM-CSF, MCP-1, MIP-1α, MIP-1β, RANTES, and KC, and markedly blocked NF-κB expression in stimulated RAW264.7 macrophages. Our findings provide new insights for understanding the mechanism involved in the anti-inflammatory effect of artepillin C and support the application of Brazilian green propolis in complementary and alternative medicine.

PHARMACOGNOSTIC, ANTIOXIDANT AND ACUTE TOXICITY STUDY OF Ficus sycomorus (Linn) (Moraceae) ROOT AND STEM BARK

2020 ◽  
Vol 4 (2) ◽  
pp. 605-614
Author(s):  
Murtala M. Namadina ◽  
H. Haruna ◽  
U. Sanusi
Keyword(s):  
Acute Toxicity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
The Body ◽  
Toxicity Study ◽  
Bark Extract ◽  
Root Extract ◽  
Acute Toxicity Study ◽  
Radical Scavenging Ability ◽  
Phytochemical Components

Most of biochemical reactions in the body generates Reactive Oxygen Species (ROS), which are involved in the pathogenesis of oxidative stress-related disorders like diabetes, nephrotoxicity, cancer, cardiovascular disorders, inflammation and neurological disorders when they attack biochemical molecules like proteins, lipids and nucleic acid. Antioxidants are used to protect the cells or tissues against potential attack by ROS. Most medicinal plants possess a rich source of antioxidants such as flavonoids, phenols, tannins, alkaloids among others. These phytochemicals are currently pursued as an alternative and complimentary drug. In this study, phytochemical components, antioxidant and acute toxicity study of the methanol extract of stem bark and root of F. sycomorus were carried out using standard methods. Findings from this study revealed the presence of some diagnostic microscopical features such as calcium oxalate, starch, gum/mucilage, lignin, Aleurone grain, suberized/Cuticular cell wall and inulin but calcium carbonate was absent in stem bark but present in the powdered root. Quantitative physical constants include moisture contents (6.40% and 7.82%), ash value (7.20% and 9.30 %) in stem bark and root respectively. Carbohydrates, alkaloid, flavonoids, saponins, tannins, glycoside, steroid, triterpenes and phenols were present in all the extracts. They were found to exhibit potent 1,1,-diphenyl 2-picryl hydrazyl (DPPH) free scavenging activity. The DPPH radical scavenging ability of the extracts showed the following trend Ascorbic acid < stem bark extract˃ root extract. The LD50 of the methanolic stem bark and root extracts were found to be greater than 5000 mg /kg and is considered safe for use. Nonetheless, further

Capillary Electrophoresis Separation of Artepillin C: Determination in Brazilian Green Propolis

2021 ◽  
Author(s):  
Fabio Galeotti ◽  
Federica Capitani ◽  
Francesca Maccari ◽  
Veronica Mantovani ◽  
Nicola Volpi
Keyword(s):  
Capillary Electrophoresis ◽  
Voltage Gradient ◽  
Food Ingredients ◽  
Therapeutic Applications ◽  
Optimal Separation ◽  
Artepillin C ◽  
Brazilian Propolis ◽  
Main Component ◽  
Electrophoresis Separation ◽  
Brazilian Green Propolis

Abstract Propolis is important in complementary and alternative medicine having well-known therapeutic applications. Artepillin C, a main component of Brazilian (green) propolis, has attracted great attention for its anticancer action. Consequently, the synthesis of artepillin C has been reported but, due to the limited yield and elevated costs, this biomolecule is largely produced from Brazilian propolis. We report the capillary electrophoresis (CE) separation of artepillin C in Brazilian propolis also comparing the results with those of HPLC-UV-MS. Optimal separation was obtained with a simple buffer constituted of sodium tetraborate 30 mM pH 9.2 and detection at 210 nm. Artepillin C and the polyphenols of propolis were fully separated with a voltage gradient of 30 to 8 kV and a current of 300 μA for a total run of 50 min. The sensitivity of CE-UV was 22 times greater than HPLC-UV and 100 times more than HPLC-MS with also a stronger reduction in the run time and a greater robustness and reproducibility. The development of CE as an effective and reliable method for the analysis of artepillin C is desired as the standardized quality controls are essential before propolis or its biomolecules can be adopted routinely in nutraceuticals, food ingredients and therapeutic applications.

scholarly journals Preparation of Antioxidant and Antibacterial Chitosan Film from Periplaneta americana

Insects ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 53
Author(s):  
Sicong Chen ◽  
Xunfan Wei ◽  
Zhuoxiao Sui ◽  
Mengyuan Guo ◽  
Jin Geng ◽  
...  
Keyword(s):  
Food Packaging ◽  
Periplaneta Americana ◽  
Uv Light ◽  
Radical Scavenging ◽  
Chitosan Film ◽  
American Cockroach ◽  
Raw Material ◽  
Potential Resource ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Among different insects, the American cockroach (Periplaneta americana) has been bred in industrial scale successfully as a potential resource of protein, lipid, and antibacterial peptide. However, the application of its chitosan has not been studied widely, which has hindered the sufficient utilization of P. americana. In this paper, the chitosan from P. americana was separated, characterized, and processed into film (PaCSF) to examine its potential of being applied in food packaging. As the results of different characterizations showed, PaCSF was similar to shrimp chitosan film (SCSF). However, concerning the performances relating to food packaging, the two chitosan films were different. PaCSF contained more water (42.82%) than SCSF did, resulting in its larger thickness (0.08 mm). PaCSF could resist UV light more effectively than SCSF did. Concerning antioxidant activity, the DPPH radical scavenging ability of PaCSF increased linearly with time passing, reaching 72.46% after 8 h, which was better than that of SCSF. The antibacterial activity assay exhibited that PaCSF resisted the growth of Serratia marcescens and Escherichia coli more effectively than SCSF did. The results implied that P. americana chitosan could be a potential raw material for food packaging, providing a new way to develop P. americana.

scholarly journals Oxygen electrode as a new tool to evaluate hydroxyl radical-scavenging ability

2008 ◽  
Vol 134 (2) ◽  
pp. 516-520 ◽  
Author(s):  
Keiko Komagoe ◽  
Hiroaki Takeuchi ◽  
Takashi Katsu
Keyword(s):  
Hydroxyl Radical ◽  
Radical Scavenging ◽  
Oxygen Electrode ◽  
Radical Scavenging Ability

scholarly journals Radical Scavenging Ability by ESR Spin Trapping and Oxygen Radical Absorbance Capacity of Hot Water Extracts from Mushrooms

2013 ◽  
Vol 60 (4) ◽  
pp. 173-178 ◽  
Author(s):  
Tomomi Kanno ◽  
Shouei Kawamura ◽  
Etsuko Harada ◽  
Hiromi Kameya ◽  
Mitsuko Ukai ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Spin Trapping ◽  
Oxygen Radical ◽  
Hot Water ◽  
Oxygen Radical Absorbance Capacity ◽  
Water Extracts ◽  
Esr Spin Trapping ◽  
Radical Scavenging Ability

scholarly journals Radical Scavenging Ability in Mugi (Barley)-miso

1998 ◽  
Vol 93 (10) ◽  
pp. 841-843 ◽  
Author(s):  
Yasuo HAYASHIDA ◽  
Kenryo NISHIMURA ◽  
Colin J. SLAUGHTER
Keyword(s):  
Radical Scavenging ◽  
Radical Scavenging Ability
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