Association of Pseudomonas putida formaldehyde dehydrogenase with superparamagnetic nanoparticles: an effective way of improving the enzyme stability, performance and recycling

2015 ◽  
Vol 39 (3) ◽  
pp. 2162-2167 ◽  
Author(s):  
Caterina G. C. M. Netto ◽  
Leandro H. Andrade ◽  
Henrique E. Toma
Keyword(s):  
Magnetic Nanoparticles ◽  
Chemical Modification ◽  
Pseudomonas Putida ◽  
Enzyme Stability ◽  
Superparamagnetic Nanoparticles ◽  
Formaldehyde Dehydrogenase ◽  
Stability Performance ◽  
Modification Process

The association of formaldehyde dehydrogenase fromPseudomonas putida(FalDH) with two different magnetic nanoparticles led to distinct enzyme responses depending upon the chemical modification process applied.

Formaldehyde Dehydrogenase from Pseudomonas putida: A Zinc Metalloenzyme

1984 ◽  
Vol 96 (5) ◽  
pp. 1587-1591 ◽  
Author(s):  
Susumu ŌGUSHI ◽  
Makoto ANDO ◽  
Daisuke TSURU
Keyword(s):  
Pseudomonas Putida ◽  
Formaldehyde Dehydrogenase

scholarly journals Palm Olein as Renewable Raw Materials for Industrial and Pharmaceutical Products Applications: Chemical Characterization and Physicochemical Properties Studies

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Darfizzi Derawi ◽  
Bashar Mudhaffar Abdullah ◽  
Hasniza Zaman Huri ◽  
Rahimi M. Yusop ◽  
Jumat Salimon ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Palm Olein ◽  
Raw Materials ◽  
Viscosity Index ◽  
Chemical Characterization ◽  
Pharmaceutical Products ◽  
Raw Material ◽  
Chemical Compositions ◽  
Renewable Raw Materials ◽  
Modification Process

Palm olein (POo) is widely produced as edible oil in tropical countries. POois considered as renewable raw material for the new industrial and pharmaceutical products synthesis based on its characterization. Palm olein was good on its viscosity index, oxidative stability, and flash and fire point. POocontained unsaturated triacylglycerols (TAGs): POO (33.3%); POP (29.6%) which plays an important role in chemical modification process to produce new industrial products. The double bond was detected on1H-NMR (5.3 ppm) and13C-NMR (130 ppm) spectra. The chemical compositions of POowere tested by using high performance liquid chromatography (HPLC) and gas chromatography (GC) techniques. This unsaturated oil is potentially to be used as renewable raw materials in chemical modification process to synthesise polyols, polyurethane, and biolubricant for industrial and pharmaceutical products application.

scholarly journals Magnetosensibility and Magnetic Properties of Ectatomma brunneun Smith, F. 1858 Ants

Sociobiology ◽  
2021 ◽  
Vol 68 (1) ◽  
pp. 5188
Author(s):  
Márlon César Pereira ◽  
Maria da Graça Cardoso Pereira-Bomfim ◽  
Ingrid De Carvaho Guimarães ◽  
Candida Anitta Pereira Rodrigues ◽  
Jilder Peña Serna ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Magnetic Nanoparticles ◽  
Geomagnetic Field ◽  
Nestmate Recognition ◽  
The Body ◽  
Superparamagnetic Nanoparticles ◽  
Behavioral Repertoire ◽  
Behavior Study ◽  
The Social ◽  
First Time

The aim of the present paper is to study magnetosensibility and to seek for magnetic nanoparticles in ants. The social insects, by living in colonies, developed very efficient methods of nestmate recognition, being less tolerant towards individuals from other colonies. Therefore, any kind of strange behavior between nestmates and/or conspecifics, besides those present in their own behavioral repertoire, is not expected. The behavior study in the present paper analyze whether changes in the intensity of applied magnetic fields on Ectatomma brunneun (Smith) ants can cause changes in the normal pattern of interaction between conspecifics. A pair of coils generating a magnetic field was used to change the whole local geomagnetic field. Magnetometry studies were done on abdomens and head + antennae using a SQUID magnetometer. The results show that changes in the geomagnetic field affect the usual pattern of interactions between workers from different colonies. The magnetometry results show that abdomens present superparamagnetic nanoparticles and heads present magnetic single domain nanoparticles. Behavior experiments show for the first time that Ectatomma brunneun ants are magnetosensible. The change in nestmate recognition of Ectatomma ants observed while a magnetic field is applied can be associated to some kind of disturbance in a magnetosensor presented in the body based on magnetic nanoparticles.

scholarly journals Synthesis of Poly-Sodium-Acrylate (PSA)-Coated Magnetic Nanoparticles for Use in Forward Osmosis Draw Solutions

Nanomaterials ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 1238 ◽  
Author(s):  
Irena Ban ◽  
Sabina Markuš ◽  
Sašo Gyergyek ◽  
Miha Drofenik ◽  
Jasmina Korenak ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Magnetic Nanoparticles ◽  
External Magnetic Field ◽  
Osmotic Pressure ◽  
Forward Osmosis ◽  
Superparamagnetic Nanoparticles ◽  
Molar Ratio ◽  
Sodium Acrylate ◽  
Molar Ratios ◽  
Magnetite Phase

The synthesis of magnetic nanoparticles (MNPs) coated with hydrophilic poly-sodium-acrylate (PSA) ligands was studied to assess PSA-MNP complexes as draw solution (DS) solutes in forward osmosis (FO). For MNP-based DS, the surface modification and the size of the MNPs are two crucial factors to achieve a high osmolality. Superparamagnetic nanoparticles (NP) with functional groups attached may represent the ideal DS where chemical modifications of the NPs can be used in optimizing the DS osmolality and the magnetic properties allows for efficient recovery (DS re-concentration) using an external magnetic field. In this study MNPs with diameters of 4 nm have been prepared by controlled chemical co-precipitation of magnetite phase from aqueous solutions containing suitable salts of Fe2+ and Fe3+ under inert atmosphere and a pure magnetite phase could be verified by X-ray diffraction. Magnetic colloid suspensions containing PSA-coated MNPs with three different molar ratios of PSA:MNP = 1:1, 1:2 and 1:3 were prepared and assessed in terms of osmotic pressure, aggregation propensity and magnetization. Fourier Transform Infrared Spectroscopy (FTIR) confirmed the presence of PSA on coated MNPs and pristine PSA-MNPs with a molar ratio PSA:MNP = 1:1 exhibited an osmotic pressure of 30 bar. Molar ratios of PSA:MNP = 1:2 and 1:3 lead to the formation of less stabile magnetic colloid solutions, which led to the formation of aggregates with larger average hydrodynamic sizes and modest osmotic pressures (5.5 bar and 0.2 bar, respectively). After purification with ultrafiltration, the 1:1 nanoparticles exhibited an osmotic pressure of 9 bar with no aggregation and a sufficient magnetization of 25 emu/g to allow for DS regeneration using an external magnetic field. However, it was observed that the amount of PSA molecules attached to the MNPs decreased during DS recycling steps, leaving only strong chelate-bonded core-shell PSA as coating on the MNPs. This demonstrates the crucial role of MNP coating robustness in designing an efficient MNP-based DS for FO.

Preparation and characterization of Pseudomonas putida esterase immobilized on magnetic nanoparticles

2006 ◽  
Vol 39 (5) ◽  
pp. 1089-1095 ◽  
Author(s):  
Shyh-Yu Shaw ◽  
Yu-Jen Chen ◽  
Jung-Jung Ou ◽  
Lewis Ho
Keyword(s):  
Magnetic Nanoparticles ◽  
Pseudomonas Putida

scholarly journals Nanoreactors based on DNAzyme-functionalized magnetic nanoparticles activated by magnetic field

Nanoscale ◽  
2018 ◽  
Vol 10 (3) ◽  
pp. 1356-1365 ◽  
Author(s):  
Saira F. Bakshi ◽  
Nataliia Guz ◽  
Andrey Zakharchenko ◽  
Han Deng ◽  
Alexei V. Tumanov ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Magnetic Nanoparticles ◽  
Cancer Biomarkers ◽  
Superparamagnetic Nanoparticles ◽  
Functionalized Magnetic Nanoparticles

A new biomimetic nanoreactor for analysis of mRNA cancer biomarkers is presented based on DNAzyme bound to superparamagnetic nanoparticles.

scholarly journals Immobilization of carboxypeptidase from Sulfolobus solfataricus on magnetic nanoparticles improves enzyme stability and functionality in organic media

2014 ◽  
Vol 14 (1) ◽  
pp. 82 ◽  
Author(s):  
Silvia Sommaruga ◽  
Elisabetta Galbiati ◽  
Jesus Peñaranda-Avila ◽  
Chiara Brambilla ◽  
Paolo Tortora ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Enzyme Stability ◽  
Sulfolobus Solfataricus ◽  
Organic Media

scholarly journals PEGylated versus non-PEGylated magnetic nanoparticles as camptothecin delivery system

2014 ◽  
Vol 5 ◽  
pp. 1312-1319 ◽  
Author(s):  
Paula M Castillo ◽  
Mario de la Mata ◽  
Maria F Casula ◽  
José A Sánchez-Alcázar ◽  
Ana P Zaderenko
Keyword(s):  
Magnetic Nanoparticles ◽  
Water Solubility ◽  
Cancer Cell Line ◽  
Lung Cancer Cell Line ◽  
Superparamagnetic Nanoparticles ◽  
Bioactive Molecules ◽  
Effective Administration ◽  
Natural Alkaloid ◽  
Apoptotic Activity ◽  
Average Size

Camptothecin (CPT; (S)-(+)-4-ethyl-4-hydroxy-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14-(4H,12H)-dione) is a highly cytotoxic natural alkaloid that has not yet found use as chemotherapeutic agent due to its poor water-solubility and chemical instability and, as a consequence, no effective administration means have been designed. In this work, camptothecin has been successfully loaded into iron oxide superparamagnetic nanoparticles with an average size of 14 nm. It was found that surface modification of the nanoparticles by polyethylene glycol enables loading a large amount of camptothecin. While the unloaded nanoparticles do not induce apoptosis in the H460 lung cancer cell line, the camptothecin-loaded nanoparticle formulations exhibit remarkable pro-apoptotic activity. These results indicate that camptothecin retains its biological activity after loading onto the magnetic nanoparticles. The proposed materials represent novel materials based on naturally occurring bioactive molecules loaded onto nanoparticles to be used as chemotherapeutic formulations. The procedure seems apt to be extended to other active molecules extracted from natural products. In addition, these materials offer the potential of being further implemented for combined imaging and therapeutics, as magnetic nanoparticles are known to be multifunctional tools for biomedicine.

scholarly journals Mechano-sorptive creep of Portuguese pinewood chemically modified

2014 ◽  
Vol 56 (1) ◽  
pp. 30-44
Author(s):  
Duarte Barroso Lopes ◽  
Carsten Mai
Keyword(s):  
Cell Wall ◽  
Chemical Modification ◽  
Experimental Work ◽  
Creep Behaviour ◽  
Hydrophobic Effect ◽  
Untreated Wood ◽  
Melamine Resin ◽  
Chemically Modified ◽  
Modification Process ◽  
Better Than

Summary The effect of chemical modification on mechano-sorptive creep in bending was studied by experimental work. Stakes with 20 × 20 × 400 mm RTL of Portuguese wood species (Pinus pinaster Aiton) modified with 1,3-dimethylol-4,5- dihydroxyethyleneurea (DMDHEU), m-methylated melamine resin (MMF), tetraethoxysilane (TEOS) and amid wax (WA) were measured under asymmetric moistening conditions over a period of 42 days (app. 1000 hours) with stress level (SL) of 12 MPa, according to ENV 1156. The cell wall treatments (DMDHEU and MMF resins) had shown significant reduction of creep (creep factors, kc) when compared to untreated wood under similar conditions. Both types of resins and levels of treatments (different WPG) did not shown significant effects. In the lumen fill treatments, deposit material of TEOS did not affected the creep behaviour (kc); Wax treatment was shown a particular compliance of creep due to avoid exchange moisture (by the hydrophobic effect of wax). The anti-creep efficiency (ACE) correlated better than other mechanical or physical properties imparted by the modification process

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