scholarly journals Simple and rapid quantification of phospholipids for supramolecular membrane transport assays

2016 ◽  
Vol 14 (7) ◽  
pp. 2182-2185 ◽  
Author(s):  
Robert Hein ◽  
Can B. Uzundal ◽  
Andreas Hennig
Keyword(s):  
Membrane Transport ◽  
Phospholipid Content ◽  
H Nmr ◽  
Nmr Method ◽  
Rapid Quantification

We introduce a simple 1H NMR method for quantification of the phospholipid content of liposomes.

Synthesis of a sequence-controlled in-chain alkynyl/tertiary amino dual-functionalized terpolymer via living anionic polymerization

2018 ◽  
Vol 9 (1) ◽  
pp. 108-120 ◽  
Author(s):  
Lincan Yang ◽  
Hongwei Ma ◽  
Li Han ◽  
Xinyu Hao ◽  
Pibo Liu ◽  
...  
Keyword(s):  
Anionic Polymerization ◽  
Kinetic Characteristics ◽  
Living Anionic Polymerization ◽  
Sequence Structure ◽  
H Nmr ◽  
Nmr Method ◽  
Tertiary Amino

A dual-functionalized sequence-defined terpolymer was synthesized via living anionic polymerization; meanwhile its kinetic characteristics and sequence structure were investigated in detail via the in situ1H NMR method.

The assignment of the configuration for α-hydroxy acid esters using a CEC strategy

2016 ◽  
Vol 14 (23) ◽  
pp. 5258-5262 ◽  
Author(s):  
Ruixue Peng ◽  
Lili Lin ◽  
Yuheng Zhang ◽  
Wangbin Wu ◽  
Yan Lu ◽  
...  
Keyword(s):  
Absolute Configuration ◽  
Hydroxy Acid ◽  
H Nmr ◽  
Nmr Method ◽  
The Absolute ◽  
Acid Esters

A simple and efficient 1H NMR method for determining the absolute configuration of chiral α-hydroxy acid esters using a CEC strategy with a chiral N,N′-dioxide-scandium(iii) complex was developed.

Revision of the structure of 3-methoxy-14α-hydroxy-D-homo-1,3,5-(10)-estratrien-17a-one. A simple 1H NMR method for the determination of configuration of hydroxy group in position 5 and/or 14 of the D-homo-steroid skeleton

1991 ◽  
Vol 56 (7) ◽  
pp. 1512-1524 ◽  
Author(s):  
Miloš Buděšínský ◽  
Alexander Kasal ◽  
Želimír Procházka ◽  
Huynh Kim Thoa ◽  
Soňa Vašíčková ◽  
...  
Keyword(s):  
Hydroxy Group ◽  
Chemical Shifts ◽  
Cotton Effect ◽  
Hydroxyl Group ◽  
H Nmr ◽  
Nmr Method ◽  
Synthetic Models ◽  
Steroid Skeleton

Eignerova and Prochazka found in 1974 the Cotton effect value for 3-methoxy-14α-hydroxy-D-homo-1,3,5(10)-estratrien-17a-one (Ia) to be Δε – 2.76.Calculation of the Δε value for this compound led, however, to a substantially lower value, which suggested the hypothesis that the compound was in fact rather an epimer with the hydroxy group in position 14β. This hypothesis was studied by means of 1H NMR spectra of synthetic models, using the changes of the chemical shifts of angular methyls, induced by in situ acylation of the angular hydroxyl with an α- or β-configuration with trichloroacetyl isocyanate (TAI). The observed TAI-acylation shifts on model compounds indicated the structure Ib with a 14b-configuration of the hydroxyl group. Indenpendent proof has been given by the synthesis of both 14-hydroxy epimers, Ia and Ib. A simple 1H NMR method is proposed for the determination of configuration of the hydroxyl in position 5 or 14 of D-homo-steroid skeleton.

1H NMR as a release methodology for the analysis of phospholipids and other constituents in infant nutrition

2016 ◽  
Vol 8 (41) ◽  
pp. 7493-7499 ◽  
Author(s):  
Yulia B. Monakhova ◽  
Martina Betzgen ◽  
Bernd W. K. Diehl
Keyword(s):  
1H Nmr ◽  
Infant Nutrition ◽  
H Nmr ◽  
Nmr Method

An 1H NMR method was developed to control five phospholipid (PL) species, namely, phosphatidylcholine (PC), sphingomyelin (SPH), phosphatidylethanolamine (PE), phosphatidylserine (PS) and phosphatidylinositol (PI) in infant nutrition.

Exhibition of the Brønsted acid–base character of a Schiff base in palladium(ii) complex formation: lithium complexation, fluxional properties and catalysis of Suzuki reactions in water

2015 ◽  
Vol 44 (15) ◽  
pp. 6896-6908 ◽  
Author(s):  
Rajnish Kumar ◽  
Ganesan Mani
Keyword(s):  
Palladium Complex ◽  
Palladium Complexes ◽  
Bronsted Acid ◽  
Brønsted Acid ◽  
Acid Base ◽  
X Ray ◽  
H Nmr ◽  
Suzuki Reactions ◽  
Nmr Method ◽  
Brönsted Acid

The Brønsted acid–base character of bis(iminopyrrolylmethyl)amine was shown through the X-ray structures of palladium complexes. The bischelated palladium complex is fluxional as studied by the VT 1H NMR method and effectively catalyzes Suzuki reactions in water.

scholarly journals Validation of a Generic Quantitative 1 H NMR Method for Natural Products Analysis

2013 ◽  
Vol 24 (6) ◽  
pp. 581-597 ◽  
Author(s):  
Tanja Gödecke ◽  
José G. Napolitano ◽  
María F. Rodríguez-Brasco ◽  
Shao-Nong Chen ◽  
Birgit U. Jaki ◽  
...  
Keyword(s):  
Natural Products ◽  
H Nmr ◽  
Nmr Method ◽  
Products Analysis

Conformational analysis of [3.3.3](1,3,5)cyclophane systems

1990 ◽  
Vol 68 (3) ◽  
pp. 440-445 ◽  
Author(s):  
Tatsuya Meno ◽  
Katsuya Sako ◽  
Masahiko Suenaga ◽  
Miyuki Mouri ◽  
Teruo Shinmyozu ◽  
...  
Keyword(s):  
Conformational Analysis ◽  
Variable Temperature ◽  
Conformational Behavior ◽  
Energy Barriers ◽  
H Nmr ◽  
Nmr Method

2,2,11,11,20,20-Hexadeuterio[3.3.3](1,3,5)cyclophane 1-d6 and its precursor, [3.3.3](1,3,5)cyclophane-2,11,20-trione 2, were synthesized and their conformational behavior was examined by a variable temperature 1H NMR method; 1-d6 (ca. 1% CD2Cl2 solution) exists in a mixture of the conformer A (C3h) and the conformer B (Cs) in a 27:73 ratio at −70 °C with the energy barriers for the trimethylene bridge inversion processes [Formula: see text] being 12.4 kcal/mol (Tc = −7 °C, 270 MHz). Conformer B is more stable than A by 0.4 kcal/mol based on the populations of both conformers. In contrast to the conformational behavior of 1, inversion of the tricarbon bridges of 2 is not frozen even at −90 °C because of the increased flexibility of these tricarbon bridges. Keywords: conformational analysis, cyclophane, tri-n-butyltin deuteride.

scholarly journals Simultaneous Determination of Three Coumarins in Angelica dahurica by 1H-qNMR Method: A Fast and Validated Method for Crude Drug Quality Control

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Lan Yang ◽  
Qian Li ◽  
Yanmei Feng ◽  
Daiyu Qiu
Keyword(s):  
Extraction Method ◽  
Plant Material ◽  
Limit Of Detection ◽  
Internal Standard ◽  
H Nmr ◽  
Nmr Method ◽  
Assisted Extraction ◽  
Limit Of Quantitation ◽  
Drug Quality Control

In this study, a quantitative 1H NMR method (1H-qNMR) for determining the contents of imperatorin, byakangelicin, and oxypeucedanin in A. dahurica in traditional Chinese medicine (TCM) has been established. Dried plant material was extracted exhaustively with methanol by an ultrasonication-assisted extraction method. The 1H-qNMR measurements were performed on a 600 -MHz spectrometer with hydroquinone as the internal standard reference in deuterated dimethyl sulfoxide (DMSO-d6) solvent. Quantification was carried out using the 1H resonance signals at 6.55 ppm for hydroquinone and 7.68, 7.38-7.39, and 6.38-6.39 ppm for imperatorin, byakangelicin, and oxypeucedanin, respectively. The linearity, limit of detection (LOD), limit of quantitation (LOQ), precision, reproducibility, stability, and recovery of the methodology were evaluated, and results were good. The newly developed method has been applied to determine the three coumarins in A. dahurica.

scholarly journals Subversion of membrane transport pathways by vacuolar pathogens

2011 ◽  
Vol 195 (6) ◽  
pp. 943-952 ◽  
Author(s):  
Eric Alix ◽  
Shaeri Mukherjee ◽  
Craig R. Roy
Keyword(s):  
Membrane Transport ◽  
Bacterial Infections ◽  
Host Cells ◽  
Eukaryotic Cells ◽  
Phospholipid Content ◽  
Intracellular Pathogens ◽  
Secretion Systems ◽  
Transport Pathways ◽  
Bacterial Effectors ◽  
Insight Into

Mammalian phagocytes control bacterial infections effectively through phagocytosis, the process by which particles engulfed at the cell surface are transported to lysosomes for destruction. However, intracellular pathogens have evolved mechanisms to avoid this fate. Many bacterial pathogens use specialized secretion systems to deliver proteins into host cells that subvert signaling pathways controlling membrane transport. These bacterial effectors modulate the function of proteins that regulate membrane transport and alter the phospholipid content of membranes. Elucidating the biochemical function of these effectors has provided a greater understanding of how bacteria control membrane transport to create a replicative niche within the host and provided insight into the regulation of membrane transport in eukaryotic cells.

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