scholarly journals Nanoparticles of the poly([N-(2-hydroxypropyl)]methacrylamide)-b-poly[2-(diisopropylamino)ethyl methacrylate] diblock copolymer for pH-triggered release of paclitaxel

2015 ◽  
Vol 6 (27) ◽  
pp. 4946-4954 ◽  
Author(s):  
Alessandro Jäger ◽  
Eliézer Jäger ◽  
František Surman ◽  
Anita Höcherl ◽  
Borislav Angelov ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Diblock Copolymer ◽  
Cytostatic Activity ◽  
Triggered Release ◽  
Ethyl Methacrylate ◽  
Hydroxypropyl Methacrylamide ◽  
Self Assembled

The potential of self-assembled nanoparticles for in vitro cytostatic activity has been explored on cancer cells.

X-Ray responsive nanoparticles with triggered release of nitrite, a precursor of reactive nitrogen species, for enhanced cancer radiosensitization

Nanoscale ◽  
2017 ◽  
Vol 9 (38) ◽  
pp. 14627-14634 ◽  
Author(s):  
Fang Liu ◽  
Junzhe Lou ◽  
Dimitre Hristov
Keyword(s):  
Cancer Cells ◽  
Cell Nucleus ◽  
Reactive Nitrogen Species ◽  
Nitrogen Species ◽  
Triggered Release ◽  
X Ray ◽  
Enhanced Sensitivity ◽  
New Strategy ◽  
Targeting Peptide

New strategy to enhance cancer radiotherapy: A novel gold nanosystem with surface-grafted nitroimidazole and cell nucleus-targeting peptide achieves the release of a RNS precursor, nitrite, by ionizing radiation. In vitro radiotherapy shows enhanced sensitivity of hypoxic cancer cells to X-ray radiation, presumably due to the generation of both reactive oxygen and nitrogen species.

A Versatile Activatable Fluorescence Probing Platform for Cancer Cells in Vitro and in Vivo Based on Self-Assembled Aptamer/Carbon Nanotube Ensembles

2014 ◽  
Vol 86 (18) ◽  
pp. 9271-9277 ◽  
Author(s):  
Lv’an Yan ◽  
Hui Shi ◽  
Xiaoxiao He ◽  
Kemin Wang ◽  
Jinlu Tang ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Cancer Cells ◽  
Fluorescence Probing ◽  
Self Assembled

Secondary Metabolites from a Culture of the Fungus Neosartorya pseudofischeri and Their In Vitro Cytostatic Activity in Human Cancer Cells

Planta Medica ◽  
2012 ◽  
Vol 78 (16) ◽  
pp. 1767-1776 ◽  
Author(s):  
Amnat Eamvijarn ◽  
Anake Kijjoa ◽  
Céline Bruyère ◽  
Véronique Mathieu ◽  
Leka Manoch ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Cancer Cells ◽  
Human Cancer ◽  
Cytostatic Activity ◽  
Human Cancer Cells

scholarly journals Doxorubicin-Loaded Mixed Micelles Using Degradable Graft and Diblock Copolymers to Enhance Anticancer Sensitivity

Cancers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 3816
Author(s):  
Yi-Chun Chen ◽  
Chang-Jung Chang ◽  
Ging-Ho Hsiue ◽  
Yi-Ting Chiang
Keyword(s):  
Cancer Cells ◽  
Graft Copolymer ◽  
Diblock Copolymers ◽  
Mixed Micelles ◽  
High Rate ◽  
Micellar System ◽  
Hydroxypropyl Methacrylamide ◽  
Tumor Accumulation

In this study, a graft copolymer, poly(N-(2-hydroxypropyl) methacrylamide dilactate)-co-(N-(2-hydroxypropyl) methacrylamide-co-histidine)-graft-poly(d,l-lactide), and a diblock copolymer, methoxy poly(ethylene glycol)-b-poly(d,l-lactide), were assembled into a mixed micellar system to encapsulate the anticancer drug doxorubicin (Dox). This mixed micellar system possesses the hydrophobic lactide segment of both copolymers, which reinforces its stability in physiological milieus; the histidine molecules appended on the graft copolymer provide the desired pH-responsive behavior to release Dox during internalization in cancer cells. The results demonstrate that the two copolymers were successfully prepared, and their ratios in the mixed micelles were optimized on the basis of the results of the stability tests. Under acidic conditions, the mixed micelles swell and are able to release their payloads. Therefore, the in vitro results indicate that the Dox in the mixed micelles is released effectively in response to the environmental pH of the mimetic internalization process, increasing cancer cells’ sensitivity toward Dox. The mixed micelles display low cytotoxicity due to the degradability of the polymers. The in vivo images show that the high stability of the mixed micelles ensures a high tumor accumulation. This selective tumor accumulation results in an excellent inhibition of in vivo tumor growth and a high rate of apoptosis in cancerous tissues, with low toxicity. This highly stable, mixed micellar system with a pH-dependent drug release, which enables the precise delivery of drugs to the tumor lesions, is feasible to employ clinically in cancer therapy.

scholarly journals Оценка эффективности противоопухолевого воздействия и индукции апоптоза в клетках карциномы почки человека биологически активными добавками, содержащими ресвератрол, индол-3-карбинол и кордицепин, флуоресцентными методами визуализации

2021 ◽  
Vol 129 (6) ◽  
pp. 727
Author(s):  
Н.В. Полуконова ◽  
Д.С. Исаев ◽  
А.М. Мыльников ◽  
А.Б. Бучарская ◽  
А.В. Полуконова ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Kidney Cancer ◽  
Human Tumor ◽  
Human Kidney ◽  
Antitumor Agent ◽  
Cytostatic Activity ◽  
Antitumor Effects ◽  
Human Renal Cell Carcinoma ◽  
Low Concentrations

We compared the effectiveness of antitumor effects and apoptosis induction on human renal cell carcinoma A498 extracts of cruciferous plant material (indole-3-carbinol), Chinese mushroom (cordycepin), French red wine (resveratrol) at low concentrations after 24 and 48 h using fluorescent methods of imaging apoptosis and necrosis in human tumor cells in vitro . Propidium iodide and acridine orange were used as dyes in the "alive and dead" test, which allowed us to detect the number of dead cells and cells in which apoptosis had started. It was found that indole-3-carbinol at low concentrations (0.0288 and 0.1152 mg/ml) has a pronounced cytotoxic and cytostatic activity against human kidney cancer cells, significantly exceeding the action of resveratrol at the same concentrations. At the same time, cordycepin has no cytotoxic and cytostatic activity at these concentrations. Indole-3-carbinol also has the most pronounced apoptotic activity at concentrations of 0.0144-0.1152 mg/ml, after 48 h the number of kidney cancer cells in apoptosis increases by 6.8-10 times compared to control. It is concluded that indole-3-carbinol is a promising antitumor agent for use in the complex therapy of patients with kidney cancer.

scholarly journals Docetaxel-Loaded Chitosan-Cholesterol Conjugate-Based Self-Assembled Nanoparticles for Overcoming Multidrug Resistance in Cancer Cells

Pharmaceutics ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 783
Author(s):  
Chao-Feng Mu ◽  
Fude Cui ◽  
Yong-Mei Yin ◽  
Hyun-Jong Cho ◽  
Dae-Duk Kim
Keyword(s):  
Multidrug Resistance ◽  
Zeta Potential ◽  
Cancer Cells ◽  
In Vitro Release ◽  
Spherical Shape ◽  
Entrapment Efficiency ◽  
Hydrodynamic Diameter ◽  
Anticancer Drug Delivery ◽  
Self Assembled

Cholesteryl hemisuccinate (CHS)-conjugated chitosan (CS)-based self-assembled nanoparticles (NPs) were developed for enhancing the intracellular uptake of docetaxel in multidrug resistance (MDR)-acquired cancer cells. CHS-CS was successfully synthesized and self-aggregation, particle size, zeta potential, drug entrapment efficiency, and in vitro drug release of docetaxel-loaded CHS-CS NPs were tested. The optimized NPs had a mean hydrodynamic diameter of 303 nm, positive zeta potential of 21.3 mV, and spherical shape. The in vitro release of docetaxel from the optimized CHS-CS NPs in different pH medium (pH 6.0 and 7.4) revealed that the release was improved in a more acidic condition (pH 6.0), representing a tumor cell’s environment. The superior MDR-overcoming effect of docetaxel-loaded CHS-CS NPs, compared with docetaxel solution, was verified in anti-proliferation and cellular accumulation studies in MDR-acquired KBV20C cells. Thus, CHS-CS NPs could be potentially used for overcoming the MDR effect in anticancer drug delivery.

scholarly journals Evolution from Covalent to Self-Assembled PAMAM-Based Dendrimers as Nanovectors for siRNA Delivery in Cancer by Coupled in Silico-Experimental Studies. Part II: Self-Assembled siRNA Nanocarriers

Pharmaceutics ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 324 ◽  
Author(s):  
Erik Laurini ◽  
Domenico Marson ◽  
Suzana Aulic ◽  
Maurizio Fermeglia ◽  
Sabrina Pricl
Keyword(s):  
Cancer Cells ◽  
Large Scale ◽  
Sirna Delivery ◽  
Experimental Studies ◽  
Good Manufacturing Practice ◽  
Surface Groups ◽  
Molecular Architecture ◽  
Self Assembled

In part I of this review, the authors showed how poly(amidoamine) (PAMAM)-based dendrimers can be considered as promising delivering platforms for siRNA therapeutics. This is by virtue of their precise and unique multivalent molecular architecture, characterized by uniform branching units and a plethora of surface groups amenable to effective siRNA binding and delivery to e.g., cancer cells. However, the successful clinical translation of dendrimer-based nanovectors requires considerable amounts of good manufacturing practice (GMP) compounds in order to conform to the guidelines recommended by the relevant authorizing agencies. Large-scale GMP-standard high-generation dendrimer production is technically very challenging. Therefore, in this second part of the review, the authors present the development of PAMAM-based amphiphilic dendrons, that are able to auto-organize themselves into nanosized micelles which ultimately outperform their covalent dendrimer counterparts in in vitro and in vivo gene silencing.

Isoliquiritigenin Nanosuspension Enhances Cytostatic Effects in A549 Lung Cancer Cells

Planta Medica ◽  
2020 ◽  
Vol 86 (08) ◽  
pp. 538-547 ◽  
Author(s):  
Fangxia Qiao ◽  
Yue Zhao ◽  
Yaping Mai ◽  
Jueshuo Guo ◽  
Luning Dong ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cancer Cells ◽  
A549 Cells ◽  
Cytostatic Activity ◽  
Licorice Root ◽  
Normal Cells ◽  
X Ray ◽  
Pure Drug ◽  
Pvp K30

AbstractIsoliquiritigenin, a flavonoid extracted from licorice root, has been shown to be active against most cancer cells; however, its antitumor activity is limited by its poor water solubility. The aim of this study was to develop a stable isoliquiritigenin nanosuspension for enhanced solubility and to evaluate its in vitro cytostatic activity in A549 cells. The nanosuspension of isoliquiritigenin was prepared through wet media milling with HPC SSL (hydroxypropyl cellulose-SSL) and PVP K30 (polyinylpyrrolidone-K30) as stabilizers, and the samples were then characterized according to particle size, zeta-potential, SEM (scanning electron microscopy), TEM (transmission electron microscopy), DSC (differential scanning calorimetry), XRPD (X-ray powder diffraction), FTIR (Fourier transform infrared spectroscopy), XPS (X-ray photoelectron spectroscopy), and in vitro release. The isoliquiritigenin nanosuspension prepared with HPC SSL and PVP K30 had particle sizes of 238.1 ± 4.9 nm and 354.1 ± 9.1 nm, respectively. Both nanosuspensions showed a surface charge of approximately − 20 mV and a lamelliform or ellipse shape. The dissolution of isoliquiritigenin from the 2 nanosuspensions was markedly higher than that of free isoliquiritigenin. In vitro studies on A549 cells indicated that the cytotoxicity and cellular uptake significantly improved after treatment with both nanosuspensions in comparison to the isoliquiritigenin solution. Furthermore, cell apoptosis analysis showed a 7.5 – 10-fold increase in the apoptosis rate induced by both nanosuspensions compared with pure drug. However, the cytotoxicity of pure drug and nanosuspension on normal cells (HELF) was lower, which indicated both isoliquiritigenin nanosuspensions have low toxicity to normal cells. Therefore, the isoliquiritigenin nanosuspension prepared with HPC SSL and PVP K30 as stabilizers may be a promising approach to improve the solubility and cytostatic activity of isoliquiritigenin.

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