This study reported the antioxidant activity of sappan wood (Caesalpinia sappan L.) extract and their
extract fraction from the liquid-liquid extraction and vacuum liquid chromatography. The study
comprises with phytochemical assessments, evaluation of total phenolic content (TPC) and total
flavonoid content (TFC). The evaluation of antioxidant activity was carried out by using the DPPH
method. The effectiveness of antioxidant activity in sappan wood compared to quercetin. Extract of
sappan wood (ESW) yields of 5.40%, extract fraction from liquid-liquid extraction (FE LLE) yields
of 52.15% and extract fraction from vacuum liquid chromatography (FE VLC) with silica gel and
eluent sequential from n-hexane, 5% n-hexane/95% ethyl acetate, ethyl acetate and 5% ethyl acetate/45%
methanol was produced 60 of fractions extract. The thin-layer chromatography (TLC) was analyzed
using silica gel as a stationary phase with the mobile phase of n-hexane:ethyl acetate (1:0.55) was
obtained 10 of fractions extract in fractions of 6 and 7, which showed the most positive results. The
phytochemical test indicated the presence of phenolic and flavonoids compounds. The highest values
for TPC was FE LLE (216.67 mg GAE/g), FE VLC 160.83 (mg GAE/g) and ESW (18.33 mg GAE/g),
respectively. The highest values for TFC were F VLC (152.48 mg QE/g), FE LLE (66.2 mg QE/g) and
ESW (48.4 mg QE/g), respectively. Then, the highest of antioxidants activity were F VLC (5.42 μg/mL),
FE LLE (6.41 μg/mL), quercetin (6.52 μg/mL) and ESW (7.82 μg/mL), respectively. The results showed
that the highest of TFC was related to the antioxidant activity. However, the highest value of TPC was
not showed the highest antioxidant activity. Both ESW, FE LLE and FE VLC with quercetin as control
exhibited powerful antioxidants.
Production of no-carrier-added 89Zr at an 18 MeV cyclotron, its purification and use in investigations in solvent extraction
