Multivalent gold nanoparticle–peptide conjugates for targeting intracellular bacterial infections

This study identifies and characterizes a gold nano-particle tagged antimicrobial peptide that can internalize cells and kill intracellular bacteria without being cytotoxic. It can also considerably reduceS.Typhi infection in animal model.

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Requirement of the antimicrobial peptide CRAMP for macrophages to eliminate phagocytosed E. coli through an autophagy pathway

10.1101/2020.07.23.218669 ◽

2020 ◽

Author(s):

Keqiang Chen ◽

Teizo Yoshimura ◽

Wanghua Gong ◽

Cuimeng Tian ◽

Jiaqiang Huang ◽

...

Keyword(s):

Antimicrobial Peptides ◽

Antimicrobial Peptide ◽

Bacterial Infections ◽

Active Form ◽

Intracellular Bacteria ◽

Wild Type ◽

E Coli ◽

Mouse Macrophages ◽

Autophagy Pathway ◽

Related Proteins

AbstractHost-derived antimicrobial peptides play an important role in the defense against extracellular bacterial infections. However, the capacity of antimicrobial peptides derived from macrophages as potential antibacterial effectors against intracellular pathogens remains unknown. In this study, we report that normal (wild type, WT) mouse macrophages increased their expression of the cathelicidin-related antimicrobial peptide (CRAMP) after infection by viable E. coli or stimulation with inactivated E. coli and its product LPS, a process involving activation of NF-κB followed by protease-dependent conversion of CRAMP from an inactive precursor to an active form. The active CRAMP was required by WT macrophages to eliminate phagocytosed E. coli, with participation of autophagy-related proteins ATG5, LC3-II, and LAMP-1 as well as conjugation of the bacteria with p62. The autophagy-mediated elimination of E. coli was impaired in CRAMP−/− macrophages resulting in retention of intracellular bacteria and fragmentation of macrophages. These results indicate CRAMP as a critical component in autophagy-mediated clearance of intracellular E. coli by macrophages.

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Models of In-Vivo Bacterial Infections for the Development of Antimicrobial Peptide-based Drugs

Current Topics in Medicinal Chemistry ◽

10.2174/1568026616666160713143017 ◽

2016 ◽

Vol 17 (5) ◽

pp. 613-619 ◽

Cited By ~ 11

Author(s):

Jlenia Brunetti ◽

Chiara Falciani ◽

Luisa Bracci ◽

Alessandro Pini

Keyword(s):

Antimicrobial Peptide ◽

Bacterial Infections

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HLA-B27-Associated Reactive Arthritis: Pathogenetic and Clinical Considerations

Clinical Microbiology Reviews ◽

10.1128/cmr.17.2.348-369.2004 ◽

2004 ◽

Vol 17 (2) ◽

pp. 348-369 ◽

Cited By ~ 97

Author(s):

Inés Colmegna ◽

Raquel Cuchacovich ◽

Luis R. Espinoza

Keyword(s):

Reactive Arthritis ◽

Bacterial Infections ◽

The Body ◽

Current Evidence ◽

Intracellular Bacteria ◽

Molecular Pathways ◽

Hla B27 ◽

Immune Mediated ◽

Clinical Considerations ◽

Active Bacteria

SUMMARY Current evidence supports the concept that reactive arthritis (ReA) is an immune-mediated synovitis resulting from slow bacterial infections and showing intra-articular persistence of viable, nonculturable bacteria and/or immunogenetic bacterial antigens synthesized by metabolically active bacteria residing in the joint and/or elsewhere in the body. The mechanisms that lead to the development of ReA are complex and basically involve an interaction between an arthritogenic agent and a predisposed host. The way in which a host accommodates to invasive facultative intracellular bacteria is the key to the development of ReA. The details of the molecular pathways that explain the articular and extra-articular manifestations of the disease are still under investigation. Several studies have been done to gain a better understanding of the pathogenesis of ReA; these constitute the basis for a more rational therapeutic approach to this disease.

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Gold nanoparticle-DNA aptamer-assisted delivery of antimicrobial peptide effectively inhibits Acinetobacter baumannii infection in mice

The Journal of Microbiology ◽

10.1007/s12275-022-1620-3 ◽

2021 ◽

Vol 60 (1) ◽

pp. 128-136

Author(s):

Jaeyeong Park ◽

Eunkyoung Shin ◽

Ji-Hyun Yeom ◽

Younkyung Choi ◽

Minju Joo ◽

...

Keyword(s):

Antimicrobial Peptide ◽

Acinetobacter Baumannii ◽

Gold Nanoparticle ◽

Dna Aptamer ◽

Assisted Delivery

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Deciphering the Role of Intramolecular Networking in Cholic Acid–Peptide Conjugates on the Lipopolysaccharide Surface in Combating Gram-Negative Bacterial Infections

Journal of Medicinal Chemistry ◽

10.1021/acs.jmedchem.8b01357 ◽

2019 ◽

Vol 62 (4) ◽

pp. 1875-1886 ◽

Cited By ~ 7

Author(s):

Kavita Yadav ◽

Sandeep Kumar ◽

Deepakkumar Mishra ◽

Mohammad Asad ◽

Madhurima Mitra ◽

...

Keyword(s):

Cholic Acid ◽

Bacterial Infections ◽

Peptide Conjugates ◽

Gram Negative

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Potential role of the antimicrobial peptide Tachyplesin III against multidrug-resistant P. aeruginosa and A. baumannii coinfection in an animal model

Infection and Drug Resistance ◽

10.2147/idr.s217020 ◽

2019 ◽

Vol Volume 12 ◽

pp. 2865-2874 ◽

Cited By ~ 2

Author(s):

Jialong Qi ◽

Ruiyu Gao ◽

Cunbao Liu ◽

Bin Shan ◽

Fulan Gao ◽

...

Keyword(s):

Animal Model ◽

Antimicrobial Peptide ◽

Potential Role ◽

Multidrug Resistant

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Interaction of human hematopoietic stem cells with bacterial pathogens

Blood ◽

10.1182/blood-2002-03-0898 ◽

2002 ◽

Vol 100 (10) ◽

pp. 3703-3709 ◽

Cited By ~ 40

Author(s):

Annette Kolb-Mäurer ◽

Martin Wilhelm ◽

Florian Weissinger ◽

Eva-Bettina Bröcker ◽

Werner Goebel

Keyword(s):

Stem Cells ◽

Hematopoietic Stem Cells ◽

Bacterial Infections ◽

Intracellular Bacteria ◽

Monocytic Differentiation ◽

Hematopoietic Stem ◽

Latex Beads ◽

Specific Growth Factor ◽

Bacterial Uptake ◽

Human Hscs

Primitive hematopoietic stem cells (HSCs) in the bone marrow are rare pluripotent cells with the capacity to give rise to all lineages of blood cells. During commitment, progenitor cells are composed mainly of cells with the potential for differentiation into 1 or 2 lineages. This commitment involves the acquisition of specific growth factor receptors and the loss of others. Viral and bacterial infections may lead to profound disturbance of hematopoiesis, which is possibly due to different susceptibility of HSCs to infectious agents. Here, we show that quiescent human HSCs are fully resistant to infection by the intracellular bacteria, Listeria monocytogenes andSalmonella enterica serovariationtyphimurium, and the extracellular pathogen Yersinia enterocolitica. During myeloid/monocytic differentiation induced by incubation with stem cell factor, thrombopoietin, and flt-3 ligand, partially differentiated HSCs emerge, which readily take up these pathogens and also latex beads by macropinocytosis. After further monocytic differentiation, bacterial uptake by macropinocytosis still occurs but internalization of the pathogens is now mainly achieved by receptor-mediated phagocytosis. These results suggest that in the case of HSCs uptake mechanisms for bacteria develop sequentially.

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