Human-origin Lactobacillus salivarius AR809 protects against immunosuppression in S. aureus-induced pharyngitis via Akt-mediated NF-κB and autophagy signaling pathways

2020 ◽  
Vol 11 (1) ◽  
pp. 270-284
Author(s):  
Guochao Jia ◽  
Xiaofeng Liu ◽  
Na Che ◽  
Yongjun Xia ◽  
Guangqiang Wang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Inflammatory Response ◽  
Signaling Pathways ◽  
Probiotic Strain ◽  
Human Origin ◽  
Lactobacillus Salivarius ◽  
Healthy Human ◽  
Pharyngeal Epithelium

Lactobacillus salivarius AR809 is a newly discovered probiotic strain from a healthy human pharynx and has potential ability to adhere to the pharyngeal epithelium and inhibit Staphylococcus aureus (S. aureus)-induced inflammatory response.

scholarly journals Adhesion to pharyngeal epithelium and modulation of immune response: Lactobacillus salivarius AR809, a potential probiotic strain isolated from the human oral cavity

2019 ◽  
Vol 102 (8) ◽  
pp. 6738-6749 ◽  
Author(s):  
G.C. Jia ◽  
N. Che ◽  
Y.J. Xia ◽  
Phoency F.-H. Lai ◽  
Z.Q. Xiong ◽  
...  
Keyword(s):  
Immune Response ◽  
Oral Cavity ◽  
Probiotic Strain ◽  
Lactobacillus Salivarius ◽  
Human Oral Cavity ◽  
Pharyngeal Epithelium

scholarly journals Bacteriocin production as a mechanism for the antiinfective activity of Lactobacillus salivarius UCC118

2007 ◽  
Vol 104 (18) ◽  
pp. 7617-7621 ◽  
Author(s):  
Sinéad C. Corr ◽  
Yin Li ◽  
Christian U. Riedel ◽  
Paul W. O'Toole ◽  
Colin Hill ◽  
...  
Keyword(s):  
Foodborne Pathogen ◽  
Antimicrobial Effect ◽  
Probiotic Strain ◽  
Bacteriocin Production ◽  
Human Origin ◽  
Lactobacillus Salivarius ◽  
Immunity Protein ◽  
Probiotic Strains ◽  
Stable Mutant

The mechanisms by which probiotic strains enhance the health of the host remain largely uncharacterized. Here we demonstrate that Lactobacillus salivarius UCC118, a recently sequenced and genetically tractable probiotic strain of human origin, produces a bacteriocin in vivo that can significantly protect mice against infection with the invasive foodborne pathogen Listeria monocytogenes. A stable mutant of Lb. salivarius UCC118 that is unable to produce the Abp118 bacteriocin also failed to protect mice against infection with two strains of L. monocytogenes, EGDe and LO28, confirming that bacteriocin production is the primary mediator of protection against this organism. Furthermore, Lb. salivarius UCC118 did not offer any protection when mice were infected with a strain of L. monocytogenes expressing the cognate Abp118 immunity protein AbpIM, confirming that the antimicrobial effect is a result of direct antagonism between Lb. salivarius and the pathogen, mediated by the bacteriocin Abp118.

YTHDF2 mediates LPS-induced osteoclastogenesis and inflammatory response via the NF-κB and MAPK signaling pathways

2021 ◽  
pp. 110060
Author(s):  
Caihong Fang ◽  
Mingli He ◽  
Di Li ◽  
Qiong Xu
Keyword(s):  
Inflammatory Response ◽  
Signaling Pathways ◽  
Mapk Signaling ◽  
Mapk Signaling Pathways

scholarly journals Staphylococcus aureus protein A induced inflammatory response in human corneal epithelial cells

2007 ◽  
Vol 354 (4) ◽  
pp. 955-961 ◽  
Author(s):  
Ashok Kumar ◽  
Alexander Mark Tassopoulos ◽  
Qiong Li ◽  
Fu-Shin X. Yu
Keyword(s):  
Staphylococcus Aureus ◽  
Epithelial Cells ◽  
Inflammatory Response ◽  
Protein A ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells

scholarly journals Effects of Staphylococcus aureus Bacteriophage K on Expression of Cytokines and Activation Markers by Human Dendritic Cells In Vitro

Viruses ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 617 ◽  
Author(s):  
Helen Freyberger ◽  
Yunxiu He ◽  
Amanda Roth ◽  
Mikeljon Nikolich ◽  
Andrey Filippov
Keyword(s):  
Staphylococcus Aureus ◽  
Dendritic Cells ◽  
Inflammatory Response ◽  
Adaptive Immune Response ◽  
Human Monocyte ◽  
Activation Markers ◽  
Mhc I ◽  
Adaptive Immune ◽  
Human Dendritic Cells

A potential concern with bacteriophage (phage) therapeutics is a host-versus-phage response in which the immune system may neutralize or destroy phage particles and thus impair therapeutic efficacy, or a strong inflammatory response to repeated phage exposure might endanger the patient. Current literature is discrepant with regard to the nature and magnitude of innate and adaptive immune response to phages. The purpose of this work was to study the potential effects of Staphylococcus aureus phage K on the activation of human monocyte-derived dendritic cells. Since phage K acquired from ATCC was isolated around 90 years ago, we first tested its activity against a panel of 36 diverse S. aureus clinical isolates from military patients and found that it was lytic against 30/36 (83%) of strains. Human monocyte-derived dendritic cells were used to test for an in vitro phage-specific inflammatory response. Repeated experiments demonstrated that phage K had little impact on the expression of pro- and anti-inflammatory cytokines, or on MHC-I/II and CD80/CD86 protein expression. Given that dendritic cells are potent antigen-presenting cells and messengers between the innate and the adaptive immune systems, our results suggest that phage K does not independently affect cellular immunity or has a very limited impact on it.

scholarly journals Whole-Genome Sequence of Lactobacillus salivarius DJ-sa-01, Isolated from Chicken Small Intestine

2018 ◽  
Vol 7 (14) ◽  
Author(s):  
Dongjun Kim ◽  
Mun-Ju Cho ◽  
Seungchan Cho ◽  
Yongjun Lee ◽  
Sung June Byun ◽  
...  
Keyword(s):  
Small Intestine ◽  
Genome Sequence ◽  
Probiotic Strain ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Lactobacillus Salivarius ◽  
Circular Chromosome ◽  
Content Type ◽  
Single Circular Chromosome

We have identified the whole-genome sequence of Lactobacillus salivarius DJ-sa-01, a potential probiotic strain for poultry, isolated from a chicken small intestine. We used the PacBio and Illumina platforms to obtain the sequence of the entire single circular chromosome.

scholarly journals Disease-Dependent Antiapoptotic Effects of Cannabidiol for Keratinocytes Observed upon UV Irradiation

2021 ◽  
Vol 22 (18) ◽  
pp. 9956
Author(s):  
Piotr Wójcik ◽  
Agnieszka Gęgotek ◽  
Neven Žarković ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Signaling Pathways ◽  
Antioxidant Properties ◽  
Human Keratinocytes ◽  
Caspase 8 ◽  
Apoptotic Pathway ◽  
Healthy Human ◽  
Uvb Irradiation ◽  
Apoptotic Signaling ◽  
Anti Inflammatory

Although apoptosis of keratinocytes has been relatively well studied, there is a lack of information comparing potentially proapoptotic treatments for healthy and diseased skin cells. Psoriasis is a chronic autoimmune-mediated skin disease manifested by patches of hyperproliferative keratinocytes that do not undergo apoptosis. UVB phototherapy is commonly used to treat psoriasis, although this has undesirable side effects, and is often combined with anti-inflammatory compounds. The aim of this study was to analyze if cannabidiol (CBD), a phytocannabinoid that has anti-inflammatory and antioxidant properties, may modify the proapoptotic effects of UVB irradiation in vitro by influencing apoptotic signaling pathways in donor psoriatic and healthy human keratinocytes obtained from the skin of five volunteers in each group. While CBD alone did not have any major effects on keratinocytes, the UVB treatment activated the extrinsic apoptotic pathway, with enhanced caspase 8 expression in both healthy and psoriatic keratinocytes. However, endoplasmic reticulum (ER) stress, characterized by increased expression of caspase 2, was observed in psoriatic cells after UVB irradiation. Furthermore, decreased p-AKT expression combined with increased 15-d-PGJ2 level and p-p38 expression was observed in psoriatic keratinocytes, which may promote both apoptosis and necrosis. Application of CBD partially attenuated these effects of UVB irradiation both in healthy and psoriatic keratinocytes, reducing the levels of 15-d-PGJ2, p-p38 and caspase 8 while increasing Bcl2 expression. However, CBD increased p-AKT only in UVB-treated healthy cells. Therefore, the reduction of apoptotic signaling pathways by CBD, observed mainly in healthy keratinocytes, suggests the need for further research into the possible beneficial effects of CBD.

scholarly journals Comparison of protein — A with clumping factor, hemolysis and coagulase tests for identification of Staphylococci isolated from Nasal swabs of healthy carriers

2007 ◽  
Vol 4 (1) ◽  
pp. 83-88
Author(s):  
Baghdad Science Journal
Keyword(s):  
Staphylococcus Aureus ◽  
Protein A ◽  
Blood Agar ◽  
Healthy Human ◽  
Nasal Swabs ◽  
Healthy Carriers

Thirty nine (12.8%) isolates of Staphylococcus aureus were isolated from 304 healthy human (Nasal swabs). It was found that percentage of males that have S. aureus is more than female's percentage. These isolates (39) were tested with different tests. Twenty seven isolates (69.23 %) were positive for Staphylococcus protein —A (SPA) ,thirty seven ( 94.8 %) were positive for tube coagulase , thirty five ( 89.7 % ) were positive with clumping factor and thirty two ( 82.05 %) had 13 — hemolytic on blood agar. It was found that 100% of the isolates (39 isolates) were positive with one, two or three tests (tube coagulase, clumping factor and SPA).

Sign in / Sign up

Export Citation Format

Share Document