Bioactive extracts from brewer's spent grain

2020 ◽  
Vol 11 (10) ◽  
pp. 8963-8977
Author(s):  
Teresa Bonifácio-Lopes ◽  
Ana A. Vilas Boas ◽  
Ezequiel R. Coscueta ◽  
Eduardo M. Costa ◽  
Sara Silva ◽  
...  

60% and 80% ethanolic extracts had a variety of phenolic compounds and proved to have antioxidant, antihypertensive and antibacterial activities.

LWT ◽  
2020 ◽  
Vol 133 ◽  
pp. 110136 ◽  
Author(s):  
Ingrid da Costa Maia ◽  
Carolina Thomaz dos Santos D'Almeida ◽  
Denise Maria Guimarães Freire ◽  
Elisa d'Avila Costa Cavalcanti ◽  
Luiz Claudio Cameron ◽  
...  

Author(s):  
Juan C López‐Linares ◽  
Víctor Campillo ◽  
Mónica Coca ◽  
Susana Lucas ◽  
María T García‐Cubero

Antioxidants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 265 ◽  
Author(s):  
Patricia Alonso-Riaño ◽  
María Teresa Sanz Diez ◽  
Beatriz Blanco ◽  
Sagrario Beltrán ◽  
Ester Trigueros ◽  
...  

Brewer’s spent grain (BSG) was chemically characterized obtaining 52.1% of carbohydrates, 17.8% protein, 5.9% lipids, 13.5% insoluble lignin and 24.3% of water-soluble extractives. This work has been focused on the study of polyphenol extraction of the extractive fraction by water ultrasound-assisted extraction. Selected extraction conditions were 47 °C and 21.7 mL water/gdry-BSG. The effect of solvent polarity on polyphenol extraction was studied by using ethanol aqueous mixtures, from 20% to 100% ethanol. The kinetics of polyphenol extraction have been fitted to the power law and the Weibull models yielding mean values of the root mean square deviation lower than 7.5%. Extracts have been characterized in terms of quantification of individual phenolic compounds by HPLC-DAD and protein and sugar soluble fractions (glucose, xylose, and arabinose). Polyphenol profile has been compared with other hydrolytic techniques, such as acid, basic and enzymatic hydrolysis, showing that ultrasound was not as effective as basic hydrolysis to release the phenolic acids esterified to the cell wall. A further centrifuge ultrafiltration concentration step was able to yield a retentate enriched in the protein fraction while individual phenolic compounds where mainly transferred to the permeate.


2021 ◽  
Vol 351 ◽  
pp. 129264
Author(s):  
P. Alonso-Riaño ◽  
M.T. Sanz ◽  
O. Benito-Román ◽  
S. Beltrán ◽  
E. Trigueros

2013 ◽  
Vol 61 (40) ◽  
pp. 9744-9753 ◽  
Author(s):  
Anna-Marja Aura ◽  
Piritta Niemi ◽  
Ismo Mattila ◽  
Klaus Niemelä ◽  
Annika Smeds ◽  
...  

2013 ◽  
Vol 54 (1) ◽  
pp. 382-388 ◽  
Author(s):  
Manuela M. Moreira ◽  
Simone Morais ◽  
Daniel O. Carvalho ◽  
Aquiles A. Barros ◽  
Cristina Delerue-Matos ◽  
...  

Antioxidants ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 45 ◽  
Author(s):  
Ramiro Carciochi ◽  
Carlos Sologubik ◽  
María Fernández ◽  
Guillermo Manrique ◽  
Leandro D’Alessandro

2017 ◽  
Vol 39 (3) ◽  
pp. 269 ◽  
Author(s):  
Aline Da Rosa Almeida ◽  
Márcia Regina Ferreira Geraldo ◽  
Leomara Floriano Ribeiro ◽  
Marcos Vieira Silva ◽  
Matheus Vinicius De Oliveira Brisola Maciel ◽  
...  

 Brewer's spent grain (BSG) was characterized by physicochemical, total phenolic compound and flavonoids contents. Antioxidant activity was evaluated by four different assays. The chromatographic analyses were used to quantify the phenolic compounds and the fatty acids in BSG. Ethanolic extracts were tested to evaluate antibacterial activity. The higher concentration of total phenolic compounds for BSG was obtained in the extraction with ethanol 20%. BSG showed an antioxidant potential for all tests evaluated. In the case of chromatographic analysis, phenolic acids and flavonoids, such as syringic acid and catechin, respectively, were detected in high quantities. Regarding to the fatty acids profile, polyunsaturated fatty acids, such as linoleic and oleic acids, were found in significant amounts. No antibacterial activity was reported for bacterial cultures and concentrations tested. BSG may be considered a protein source, rich in fiber, polyunsaturated fatty acids and bioactive compounds with antioxidant potential. 


2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Olga Marchut-Mikołajczyk ◽  
Piotr Drożdżyński ◽  
Arkadiusz Polewczyk ◽  
Wojciech Smułek ◽  
Tadeusz Antczak

Abstract Background Microbial surfactants called biosurfactants, thanks to their high biodegradability, low toxicity and stability can be used not only in bioremediation and oil processing, but also in the food and cosmetic industries, and even in medicine. However, the high production costs of microbial surfactants and low efficiency limit their large-scale production. This requires optimization of management conditions, including the possibility of using waste as a carbon source, such as food processing by-products. This papers describes the production and characterization of the biosurfactant obtained from the endophytic bacterial strain Bacillus pumilus 2A grown on various by-products of food processing and its potential applications in supporting plant growth. Four different carbon and nitrogen sources, pH, inoculum concentration and temperature were optimized within Taguchi method. Results Optimization of bioprocess within Taguchi method and experimental analysis revealed that the optimal conditions for biosurfactant production were brewer’s spent grain (5% w/v), ammonium nitrate (1% w/v), pH of 6, 5% of inoculum, and temperature at 30 °C, leading to 6.8 g/L of biosurfactant. Based on gas chromatography–mass spectrometry and Fourier transform infrared spectroscopy analysis produced biosurfactant was determined as glycolipid. Obtained biosurfactant has shown high and long term thermostability, surface tension of 47.7 mN/m, oil displacement of 8 cm and the emulsion index of 69.11%. The examined glycolipid, used in a concentration of 0.2% significantly enhanced growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Conclusions The endophytic Bacillus pumilus 2A produce glycolipid biosurfactant with high and long tem thermostability, what makes it useful for many purposes including food processing. The use of brewer’s spent grain as the sole carbon source makes the production of biosurfactants profitable, and from an environmental point of view, it is an environmentally friendly way to remove food processing by products. Glycolipid produced by endophytic Bacillus pumilus 2A significantly improve growth of Phaseolus vulgaris L. (bean), Raphanus L. (radish), Beta vulgaris L. (beetroot). Obtained results provide new insight to the possible use of glycolipids as plant growth promoting agents.


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