scholarly journals Proof-of-concept for 2D/CT element analysis of entire cryofrozen islets of Langerhans using a cryoloop synchrotron X-ray fluorescence setup

2020 ◽  
Vol 35 (7) ◽  
pp. 1368-1379
Author(s):  
Björn De Samber ◽  
Mohammed Bensellam ◽  
Stijn J. M. Van Malderen ◽  
Frank Seiboth ◽  
Dennis Brückner ◽  
...  
Keyword(s):  
Islets Of Langerhans ◽  
Biological Samples ◽  
Chemical Imaging ◽  
Proof Of Concept ◽  
Trace Level ◽  
Element Analysis ◽  
X Ray ◽  
Sr Xrf

This work reports on trace level chemical imaging of vitrified islets of Langerhans in 2D/CT mode using synchrotron X-ray fluorescence (SR-XRF). The newly developed method can be used for other biological samples that can be captured in a cryoloop.

Application of SR-XRF Imaging and Micro-Xanes to Meteorites, Archaeological Objects and Animal Tissues

1991 ◽  
Vol 35 (B) ◽  
pp. 1307-1315 ◽  
Author(s):  
Izumi Nakai ◽  
Atsuo Iida
Keyword(s):  
Synchrotron Radiation ◽  
Biological Samples ◽  
Chemical State ◽  
Geological Samples ◽  
Animal Tissues ◽  
Edge Structure ◽  
X Ray ◽  
Sr Xrf ◽  
Mercury Intoxication ◽  
Archaeological Objects

AbstractSynchrotron Radiation Induced X-ray Fluorescence analysis was successfully applied to the analyses of meteorites, archaeological objects and animal tissues. Electronic states of Fe and Ti in chondrules of chondrites were clarified by micro-XANES to be Fe2+ and Ti4+. Ancient iron implement (B.C. 3c A.D. 3c) with zoning of rusts was characterized by micro-XANES. Two dimensional chemical state analyses of the sample were made by selective excitation of iron. Trace element analyses of Cu, Zn, Se, and Hg in the brain and kidney of rat, mouse, and guinea pig dosed with alkyl mercury and/or Se were made from a view point of mercury intoxication. Strong correlation between Hg and Cu, Zn, Se was observed by the correlation analysis of the elemental distributions. The XRF imaging also disclosed decrease of Cu and Zn level in cancerous tissues of human kidney compared with normal ones.Advantages of Synchrotron Radiation (SR) as an X-ray source of X-ray Fluorescence (XRF) Analysis have been demonstrated by many pioneering researchers. The advantages include wavelength tunability, high intensity and high resolution, which allow us to carry out nondestructive multielemental analysis of trace elements. However, practical application of the SR-XRF is still limited in numbers. The authors expected that the application of SR-XRF is most promising in the fields of archaeological objects, geological samples, and biological samples and started the present research projects in 1987. Now we have established the analytical procedures of these samples by SR-XRF that we report a part of our results, which so far we obtained from the analyses of geological samples, archaeological objects and biological samples.The techniques of chemical state analysis used in this study are based on the following two approaches: one is XANES (X-ray absorption Near Edge Structure) and the other is SIXES (Selectively Induced X-ray Emission Spectroscopy). It is well known that the former technique together with EXAFS has been developed rapidly with the development of intense SR beams. SIXES was recently established by Sakurai et al. It enables us to carry out chemical state mapping by X-ray fluorescence using absorption edge shifts. This method was first applied to practical problems in this study.

Medium-energy microprobe station at the SXRMB of the CLS

2017 ◽  
Vol 24 (1) ◽  
pp. 333-337 ◽  
Author(s):  
Qunfeng Xiao ◽  
Aimee Maclennan ◽  
Yongfeng Hu ◽  
Mark Hackett ◽  
Peter Leinweber ◽  
...  
Keyword(s):  
Distributed Systems ◽  
Energy Range ◽  
Light Source ◽  
Biological Samples ◽  
Imaging Techniques ◽  
Chemical Imaging ◽  
Beam Size ◽  
Medium Energy ◽  
Technical Design ◽  
X Ray

Micro-XAFS and chemical imaging techniques have been widely applied for studies of heterogeneously distributed systems, mostly in hard X-ray (>5 keV) or in soft X-ray (<1.5 keV) energies. The microprobe endstation of the SXRMB (soft X-ray microcharacterization beamline) at the Canadian Light Source is optimized at the medium energy (1.7–5 keV), and it has been recently commissioned and is available for general users. The technical design and the performance (energy range, beam size and flux) of the SXRMB microprobe are presented. Examples in chemical imaging and micro-XAFS in the medium energy for important elements such as P, S and Ca for soil and biological samples are highlighted.

Artefacts in the x-ray microanalysis of frozen-hydrated biological samples

1987 ◽  
Vol 45 ◽  
pp. 658-659
Author(s):  
Patrick Echlin
Keyword(s):  
Electron Scattering ◽  
Biological Samples ◽  
Fracture Face ◽  
Detailed Structure ◽  
X Ray ◽  
Morphological Appearance ◽  
The Poor ◽  
Freeze Dried

A number of papers have appeared recently which purport to have carried out x-ray microanalysis on fully frozen hydrated samples. It is important to establish reliable criteria to be certain that a sample is in a fully hydrated state. The morphological appearance of the sample is an obvious parameter because fully hydrated samples lack the detailed structure seen in their freeze dried counterparts. The electron scattering by ice within a frozen-hydrated section and from the surface of a frozen-hydrated fracture face obscures cellular detail. (Fig. 1G and 1H.) However, the morphological appearance alone can be quite deceptive for as Figures 1E and 1F show, parts of frozen-dried samples may also have the poor morphology normally associated with fully hydrated samples. It is only when one examines the x-ray spectra that an assurance can be given that the sample is fully hydrated.

Computer programs for the calculation of x-ray intensities emitted by elements in multi-layer structures

1990 ◽  
Vol 48 (2) ◽  
pp. 216-217
Author(s):  
G.F. Bastin ◽  
H.J.M. Heijligers ◽  
J.M. Dijkstra
Keyword(s):  
Software Package ◽  
Light Element ◽  
Matrix Correction ◽  
Excellent Performance ◽  
Element Analysis ◽  
X Ray ◽  
Know How ◽  
Accurate Knowledge ◽  
Layer Structures ◽  
Bulk Matrix

For the calculation of X-ray intensities emitted by elements present in multi-layer systems it is vital to have an accurate knowledge of the x-ray ionization vs. mass-depth (ϕ(ρz)) curves as a function of accelerating voltage and atomic number of films and substrate. Once this knowledge is available the way is open to the analysis of thin films in which both the thicknesses as well as the compositions can usually be determined simultaneously.Our bulk matrix correction “PROZA” with its proven excellent performance for a wide variety of applications (e.g., ultra-light element analysis, extremes in accelerating voltage) has been used as the basis for the development of the software package discussed here. The PROZA program is based on our own modifications of the surface-centred Gaussian ϕ(ρz) model, originally introduced by Packwood and Brown. For its extension towards thin film applications it is required to know how the 4 Gaussian parameters α, β, γ and ϕ(o) for each element in each of the films are affected by the film thickness and the presence of other layers and the substrate.

An improved interference correction for trace element analysis

1992 ◽  
Vol 50 (2) ◽  
pp. 1646-1647
Author(s):  
John J. Donovan ◽  
Donald A. Snyder ◽  
Mark L. Rivers
Keyword(s):  
Trace Element Analysis ◽  
Accurate Estimate ◽  
Simple Expression ◽  
Standard Reference Materials ◽  
Characteristic Line ◽  
Analytical Line ◽  
Element Analysis ◽  
Electron Probe Analysis ◽  
Calculated Concentration ◽  
X Ray

We present a simple expression for the quantitative treatment of interference corrections in x-ray analysis. WDS electron probe analysis of standard reference materials illustrate the success of the technique.For the analytical line of wavelength λ of any element A which lies near or on any characteristic line of another element B, the observed x-ray counts at We use to denote x-ray counts excited by element i in matrix j (u=unknown; s=analytical standard; ŝ=interference standard) at the wavelength of the analytical line of A, λA (Fig. 1). Quantitative analysis of A requires an accurate estimate of These counts can be estimated from the ZAF calculated concentration of B in the unknown C,Bu measured counts at λA in an interference standard of known concentration of B (and containing no A), and ZAF correction parameters for the matrices of both the unknown and the interference standard at It can be shown that:

scholarly journals Principles of Different X-ray Phase-Contrast Imaging: A Review

2021 ◽  
Vol 11 (7) ◽  
pp. 2971
Author(s):  
Siwei Tao ◽  
Congxiao He ◽  
Xiang Hao ◽  
Cuifang Kuang ◽  
Xu Liu
Keyword(s):  
Biological Samples ◽  
Phase Contrast ◽  
Recent Progress ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray ◽  
X Ray Imaging ◽  
Internal Structures ◽  
X Ray Absorption ◽  
Made In

Numerous advances have been made in X-ray technology in recent years. X-ray imaging plays an important role in the nondestructive exploration of the internal structures of objects. However, the contrast of X-ray absorption images remains low, especially for materials with low atomic numbers, such as biological samples. X-ray phase-contrast images have an intrinsically higher contrast than absorption images. In this review, the principles, milestones, and recent progress of X-ray phase-contrast imaging methods are demonstrated. In addition, prospective applications are presented.

scholarly journals Trace element limitation of lymph nodes structure according to the x-ray fluorescent analysis with synchrotron radiation (SR XRF)

2020 ◽  
Author(s):  
O. V. Gorchakova ◽  
Yu. P. Kolmogorov ◽  
V. N. Gorchakov ◽  
G. A. Demchenko ◽  
S. N. Abdreshov
Keyword(s):  
Synchrotron Radiation ◽  
Lymph Nodes ◽  
Trace Element ◽  
X Ray ◽  
Fluorescent Analysis ◽  
Sr Xrf

scholarly journals Geochemistry and Mineralogy of Ice-Dammed Lake Sediments of the Lębork Deposit

2021 ◽  
Author(s):  
Radosław Rogoziński ◽  
Alina Maciejewska
Keyword(s):  
Chemical Composition ◽  
Lake Sediments ◽  
Inductively Coupled Plasma ◽  
Mineralogical Composition ◽  
Raw Material ◽  
Element Analysis ◽  
X Ray ◽  
Dominant Component ◽  
Clay Deposits ◽  
Dammed Lake

AbstractVarved clay deposits from ice-dammed lakes are a particularly important and broadly applied raw material used for the production of high-quality ceramics (red bricks, roof tiles, etc.), but the mineralogy and geochemistry of these sediments are not fully understood. The aim of the present study was to determine the chemical and mineralogical composition of ice-dammed lake sediments of the Lębork deposit. Major-element analysis of the compositions of selected samples from the ice-dammed lake clays was performed by X-ray fluorescence (XRF) and trace elements were determined by inductively coupled plasma-mass spectrometry. The mineralogical composition of clay samples was determined by X-ray diffraction (XRD). Analyses of the chemical composition of the ice-dammed lake clays of the Lębork deposit showed that the dominant component was SiO2 with a mean content of 56.13 wt.%; the second most abundant component was Al2O3, with a mean content for the entire deposit of 11.61 wt.%. Analysis by ICP-MS indicated the presence of rare earth elements (REE), e.g. cerium, neodymium, lanthanum, and praseodymium; their mean contents are: 56.9, 27.0, 26.3, and 7.3 ppm, respectively. Mineralogical analysis of the varved clays identified quartz, muscovite, calcite, and clay minerals – illite, kaolinite, and montmorillonite. The material filling the Lębork basin is characterized by small lateral and vertical variability in chemical composition. The results of the present study may be of considerable importance in determining the parent igneous, metamorphic, and sedimentary rocks, the weathering products of which supplied material to the ice-dammed lake, as well as in determining the mechanisms and character of the sedimentation process itself.

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