Bioinspired by both mussel foot protein and bone sialoprotein: universal adhesive coatings for the promotion of mineralization and osteogenic differentiation

2020 ◽  
Vol 11 (31) ◽  
pp. 4995-5004
Author(s):  
Xiaoling Long ◽  
Huilin Xu ◽  
Dongyue Zhang ◽  
Jianshu Li
Keyword(s):  
Osteogenic Differentiation ◽  
Bone Sialoprotein ◽  
Universal Adhesive ◽  
Natural Protein ◽  
Implant Material ◽  
Mussel Foot Protein

Natural protein bioinspired coatings are developed to promote the mineralization and osteogenic differentiation of MC3T3-E1 cells for implant material use.

scholarly journals Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured with different concentrations of prolactin

2017 ◽  
Vol 69 (6) ◽  
pp. 1573-1580
Author(s):  
K.P. Oliveira ◽  
A.M.S. Reis ◽  
A.P. Silva ◽  
C.L.R. Silva ◽  
A.M. Goes ◽  
...  
Keyword(s):  
Stem Cells ◽  
Alkaline Phosphatase ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Osteogenic Differentiation ◽  
Bone Sialoprotein ◽  
Collagen I ◽  
Female Rats ◽  
Osteogenic Potential ◽  
Vitro Effect

ABSTRACT The objective was to evaluate the in vitro effect of prolactin in osteogenic potential of adipose tissue-derived mesenchymal stem cells (ADSCs) in female rats. ADSCs were cultured in osteogenic medium with and without the addition of prolactin and distributed into three groups: 1) ADSCs (control), 2) ADSCs with addition of 100ng/mL of prolactin and 3) ADSCs with addition of 300ng/mL of prolactin. At 21 days of differentiation, the tests of MTT conversion into formazan crystals, percentage of mineralized nodules and cells per field and quantification of genic transcript for alkaline phosphatase, osteopontin, osteocalcin, bone sialoprotein, BMP-2 and collagen I by real-time RT-PCR were made. The addition of prolactin reduced the conversion of MTT in group 3 and increased the percentage of cells per field in the groups 2 and 3, however without significantly increasing the percentage of mineralized nodules and the expression of alkaline phosphatase, osteopontin, osteocalcin, bone sialoprotein, BMP-2 and collagen I. In conclusion, the addition of prolactin in concentrations of 100ng/mL and 300ng/mL does not change the osteogenic differentiation to the ADSCs of female rats despite increase in the cellularity of the culture.

scholarly journals Bioactive natural protein–hydroxyapatite nanocarriers for optimizing osteogenic differentiation of mesenchymal stem cells

2016 ◽  
Vol 4 (20) ◽  
pp. 3555-3561 ◽  
Author(s):  
Z. Z. Ding ◽  
Z. H. Fan ◽  
X. W. Huang ◽  
S. M. Bai ◽  
D. W. Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sustained Release ◽  
Osteogenic Differentiation ◽  
Drug Loading ◽  
Composite Nanoparticles ◽  
Core Shell ◽  
Loading Capacity ◽  
Natural Protein

Core–shell HA–silk composite nanoparticles as BMP-2 carriers show superior drug loading capacity and sustained release. The improved osteo-differentiation of stem cells on the carriers suggested a promising approach to designing bioactive nanocarrier systems with enhanced functions.

scholarly journals In vitro bone sialoprotein-I expression in combined gingival stromal cells and platelet rich fibrin during osteogenic differentiation

2019 ◽  
Vol 17 (12) ◽  
pp. 2341 ◽  
Author(s):  
Alexander Patera Nugraha ◽  
Ida Bagus Narmada ◽  
Diah Savitri Ernawati ◽  
Aristika Dinaryanti ◽  
Eryk Hendrianto ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Sialoprotein ◽  
Platelet Rich Fibrin

scholarly journals Induction of Osteogenic Differentiation of Mesenchymal Stem Cells by Bioceramic Root Repair Material

Materials ◽  
2019 ◽  
Vol 12 (14) ◽  
pp. 2311 ◽  
Author(s):  
Hadeel Y. Edrees ◽  
Sawsan T.H. Abu Zeid ◽  
Hazem M. Atta ◽  
Mehal A. AlQriqri
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Alkaline Phosphatase ◽  
Mesenchymal Stem Cells ◽  
Cell Viability ◽  
Osteogenic Differentiation ◽  
Bone Sialoprotein ◽  
Bone Morphogenetic Protein 2 ◽  
Repair Material ◽  
Morphogenetic Protein

This study aimed to evaluate the osteogenic activity of Endosequence Root Repair Material (ERRM) putty using rat mesenchymal stem cells (MSCs). The extract of set ERRM and ProRoot-mineral trioxide aggregate (MTA) (control) was cocultured with rat MSCs and incubated for one, three, and seven days. The cell viability and proliferation were assessed. A quantitative real-time polymerase chain reaction for bone morphogenetic protein-2 (BMP-2), alkaline phosphatase, bone sialoprotein, and osteocalcin gene expression was performed. Both materials enhanced cell viability and proliferation, which increased over time. On day seven, the cells treated with either material exhibited significantly greater cell viability compared with control untreated cells. MSCs treated with either material showed deeper alkaline phosphatase staining after three days compared to control untreated cells. Treated MSCs also exhibited upregulation of the gene expression of bone morphogenetic protein-2, alkaline phosphatase, bone sialoprotein, and osteocalcin. Both ERRM and ProRoot-MTA enhance the osteogenic differentiation of MSCs.

scholarly journals Antibacterial activity, bio-compatibility and osteogenic differentiation of graphene oxide coating on 3D-network poly-ether-ether-ketone for orthopaedic implants

2021 ◽  
Vol 32 (11) ◽  
Author(s):  
Cui Guo ◽  
Ran Lu ◽  
Xin Wang ◽  
Su Chen
Keyword(s):  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Osteogenic Differentiation ◽  
Antibacterial Properties ◽  
Orthopaedic Implant ◽  
Implant Material ◽  
3D Network ◽  
Ether Ether Ketone ◽  
Poly Ether Ether Ketone ◽  
Ether Ketone

AbstractPoly-ether-ether-ketone (PEEK) has attracted increasing attention as a promising orthopaedic implant material owing to its excellent mechanical properties and biocompatibility. However, its antibacterial properties must be improved as an implant material. In this study, PEEK was sulfonated to obtain a porous surface, and graphene oxide (GO) was deposited to form a coating with antibacterial activity and biocompatibility. After PEEK was sulfonated for different durations, GO was deposited on the surface to prepare the coating (SPEEK-GO), which was then characterised using scanning electron microscopy (SEM), Raman spectroscopy, and contact angle measurements. The in vitro study included antimicrobial and cellular tests. The results showed that the PEEK sulfonated using a 10-min treatment exhibited a uniform porous structure and provided a better basal surface for the deposition of GO. The SPEEK-GO coating displayed strong antibacterial activity against two common dental pathogens. It exhibited good adhesion and proliferation of MC3T3-E1. Moreover, it showed osteogenic differentiation as bone implant material.

Bone sialoprotein mRNA and protein expression in human multiple myeloma cell lines and patients

2000 ◽  
Vol 111 (4) ◽  
pp. 1118-1121 ◽  
Author(s):  
A. Bellahcene ◽  
I. Van Riet ◽  
C. de Greef ◽  
N. Antoine ◽  
M. F. Young ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Protein Expression ◽  
Cell Lines ◽  
Myeloma Cell ◽  
Bone Sialoprotein ◽  
Multiple Myeloma Cell ◽  
Human Multiple Myeloma ◽  
Mrna And Protein Expression

Técnica de mascaramento de substrato escurecido com lente de contato dental cimentada sobre faceta menos translúcida

2020 ◽  
Vol 10 (37) ◽  
pp. 38-45
Author(s):  
Lara Pepita de Souza Oliveira ◽  
Rebeka de Oliveira Reis ◽  
Ely Moacyr de Souza Portela ◽  
Sybilla Torres Dias ◽  
Cristiane Maria Brasil Leal
Keyword(s):  
Phosphoric Acid ◽  
Composite Resin ◽  
Intermediate Structure ◽  
Dental Ceramics ◽  
Central Incisor ◽  
Universal Adhesive ◽  
Patient Reported ◽  
Satisfaction With Treatment ◽  
Natural Teeth ◽  
Smile Aesthetics

Dental ceramics have an excellent ability to reproduce natural teeth appearance, but once the substrate has an altered coloration, it is necessary to mask this intercurrence, considering the characteristics of the ceramic and cement selected. This article aims to present a clinical case of rehabilitation with ceramic laminates to mask a darkened central incisor by planning a more opaque porcelain structure under a translucent contact lens. A female patient sought dental care complaining of dissatisfaction with smile aesthetics. It was clinically identified the presence of a Class IV darkened restoration in composite resin in element 11. In treatment planning harmony in the shape and color of elements 11 and 21 was aimed at through lithium disilicate lenses. An opaque intermediate structure was used to cover the darkening in tooth 11. The double molding technique was performed to obtain the working model. Prior to cementing, the surfaces were treated. In ceramics, conditioning with 10% hydrofluoric acid and 37% phosphoric acid, silane application and universal adhesive; in dental substrate, conditioning with 37% phosphoric acid and application of universal adhesive were performed. In laminates definitive cementation Allcem Venner cement was chosen. Patient reported satisfaction with treatment final aesthetics. Thus, cementation technique of a translucent laminate over an opaque facet is a good option for masking substrates with darkened coloration.

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