Rational design of a FRET based nanoprobe of gold conjugated carbon dots for simultaneous monitoring and disruption of Pseudomonas aeruginosa biofilm through selective detection of virulence factor pyocyanin

2021 ◽  
Author(s):  
Chandni Sharma ◽  
Ashish K Shukla ◽  
Amitabha Acharya
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Effective Treatment ◽  
Virulence Factor ◽  
Carbon Dots ◽  
Rational Design ◽  
Selective Detection ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Different Types

Successful and effective treatment of different types of infections depends upon fast and selective identification of the microorganisms. Pseudomonas aeruginosa is a gram-negative bacterium which is involved in number of...

scholarly journals Draft Genome Sequences of Two Pseudomonas aeruginosa Isolates from the Female Urogenital Tract

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Genevieve Johnson ◽  
Carine R. Mores ◽  
Alan J. Wolfe ◽  
Catherine Putonti
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Environmental Conditions ◽  
Draft Genome ◽  
Urogenital Tract ◽  
Negative Bacterium ◽  
Genome Sequences ◽  
Gram Negative ◽  
Content Type

Pseudomonas aeruginosa is a Gram-negative bacterium that has the ability to survive in and readily adapt to a variety of environmental conditions. Here, we report 2 genome sequences of P. aeruginosa strains, UMB1046 and UMB5686, isolated from the female urogenital tract.

scholarly journals Structure and Function of the PiuA and PirA Siderophore-Drug Receptors from Pseudomonas aeruginosa and Acinetobacter baumannii

2017 ◽  
Vol 61 (4) ◽  
Author(s):  
Lucile Moynié ◽  
Alexandre Luscher ◽  
Dora Rolo ◽  
Daniel Pletzer ◽  
Antoni Tortajada ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Acinetobacter Baumannii ◽  
Rational Design ◽  
Transport Systems ◽  
Gram Negative ◽  
Content Type ◽  
Drug Conjugates ◽  
Membrane Complex ◽  
And Function ◽  
Drug Receptors

ABSTRACT The outer membrane of Gram-negative bacteria presents an efficient barrier to the permeation of antimicrobial molecules. One strategy pursued to circumvent this obstacle is to hijack transport systems for essential nutrients, such as iron. BAL30072 and MC-1 are two monobactams conjugated to a dihydroxypyridone siderophore that are active against Pseudomonas aeruginosa and Acinetobacter baumannii. Here, we investigated the mechanism of action of these molecules in A. baumannii. We identified two novel TonB-dependent receptors, termed Ab-PiuA and Ab-PirA, that are required for the antimicrobial activity of both agents. Deletion of either piuA or pirA in A. baumannii resulted in 4- to 8-fold-decreased susceptibility, while their overexpression in the heterologous host P. aeruginosa increased susceptibility to the two siderophore-drug conjugates by 4- to 32-fold. The crystal structures of PiuA and PirA from A. baumannii and their orthologues from P. aeruginosa were determined. The structures revealed similar architectures; however, structural differences between PirA and PiuA point to potential differences between their cognate siderophore ligands. Spontaneous mutants, selected upon exposure to BAL30072, harbored frameshift mutations in either the ExbD3 or the TonB3 protein of A. baumannii, forming the cytoplasmic-membrane complex providing the energy for the siderophore translocation process. The results of this study provide insight for the rational design of novel siderophore-drug conjugates against problematic Gram-negative pathogens.

scholarly journals Draft Genome Sequence of a Pseudomonas aeruginosa Strain Able To Decompose N , N -Dimethyl Formamide

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Lingyue Yan ◽  
Ming Yan ◽  
Lin Xu ◽  
Li Wei ◽  
Liting Zhang
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Genome Sequence ◽  
Draft Genome ◽  
Carbon Sources ◽  
Draft Genome Sequence ◽  
Dimethyl Formamide ◽  
Organic Chemicals ◽  
Negative Bacterium ◽  
High Concentration ◽  
Gram Negative

Pseudomonas aeruginosa is a Gram-negative bacterium, which uses a variety of organic chemicals as carbon sources. Here, we report the genome sequence of the Cu1510 isolate from wastewater containing a high concentration of N , N -dimethyl formamide.

scholarly journals Draft Genome Sequences of Pseudomonas aeruginosa Isolates from Wounded Military Personnel: TABLE 1 

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Brock A. Arivett ◽  
Dave C. Ream ◽  
Steven E. Fiester ◽  
Destaalem Kidane ◽  
Luis A. Actis
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Military Personnel ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Negative Bacterium ◽  
Genome Sequences ◽  
Gram Negative ◽  
Hospital Acquired Infections ◽  
Extensive Drug Resistance ◽  
Hospital Acquired

Pseudomonas aeruginosa , a Gram-negative bacterium that causes severe hospital-acquired infections, is grouped as an ESKAPE ( Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa , and Enterobacter species) pathogen because of its extensive drug resistance phenotypes and effects on human health worldwide. Five multidrug resistant P. aeruginosa strains isolated from wounded military personnel were sequenced and annotated in this work.

Molybdenum Binding by Pseudomonas aeruginosa

1986 ◽  
Vol 39 (8) ◽  
pp. 1205 ◽  
Author(s):  
S Stojkovski ◽  
RJ Magee ◽  
J Liesegang
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Uronic Acid ◽  
Extracellular Polysaccharide ◽  
Uronic Acids ◽  
Negative Bacterium ◽  
Molybdenum Complex ◽  
Gram Negative ◽  
Pseudomonas Aeruginosa Pao1 ◽  
Polysaccharide Layer

The uptake of molybdenum by certain bacteria hinders its role as a trace metal in the micronutrients for plant growth. The binding of molybdenum by the Gram-negative bacterium Pseudomonas aeruginosa, PAO1, has been investigated. A molybdenum complex of uronic acid, which forms in the extracellular polysaccharide layer (slime), was isolated and characterized by a variety of techniques. Comparisons with 'mimic' compounds of uronic acids suggest that Pseudomonas aeruginosa, PAO1, produces a binuclear, di-oxo-bridged magnesium salt MgMo2O4(C6H8O7)2.5H2O; this indicates the important role of uronic acids in metallic uptake by bacteria.

scholarly journals Rational Design of Potent and Selective Inhibitors of an Epoxide Hydrolase Virulence Factor from Pseudomonas aeruginosa

2016 ◽  
Vol 59 (10) ◽  
pp. 4790-4799 ◽  
Author(s):  
Seiya Kitamura ◽  
Kelli L. Hvorecny ◽  
Jun Niu ◽  
Bruce D. Hammock ◽  
Dean R. Madden ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulence Factor ◽  
Epoxide Hydrolase ◽  
Rational Design ◽  
Selective Inhibitors

scholarly journals Biodegradation α-endosulfan and α-cypermethrin by Acinetobacter schindleri B7 isolated from the microflora of grasshopper (Poecilimon tauricola)

2021 ◽  
Author(s):  
Ozlem Gur Ozdal ◽  
Omer Faruk ALGUR
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Megaterium ◽  
Biological Treatment ◽  
Minimal Salt Medium ◽  
Salt Medium ◽  
Negative Bacterium ◽  
Pesticide Degradation ◽  
Gram Negative ◽  
Degrading Bacteria ◽  
Brevibacillus Parabrevis

Abstract Extensive applications of pesticides have led to the contamination of ecosystem. Therefore, the isolation of new pesticide degrading bacteria is important. For the biodegradation of α-endosulfan and α-cypermethrin, new bacteria isolates were isolated from grasshopper (Poecilimon tauricola). According to different tests, these isolated bacteria were identified as Pseudomonas aeruginosa B5, Acinetobacter johnsonii B6, Acinetobacter schindleri B7, Bacillus megaterium B9 and Brevibacillus parabrevis B12. The first two of these bacteria have been isolated as those that can use only α-endosulfan and the last two only use α-cypermethrin. Moreover, A. schindleri B7 was determined to be able to degrade both pesticides. When glucose was added to non-sulfur medium containing α-endosulfan (100 mg/L) and minimal salt medium containing α-cypermethrin (100 mg/L), both pesticide degradation and bacterial growth was increased. As a result, A. schindleri, a new gram negative bacterium, can inevitably be used in the biological treatment of environments exposed to pesticides.

Pseudomonas aeruginosa

2020 ◽  
pp. 1041-1044
Author(s):  
G.C.K.W. Koh ◽  
Sharon J. Peacock
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Urinary Tract ◽  
Opportunistic Pathogen ◽  
Selective Advantage ◽  
Innocent Bystander ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Skin Ulcers ◽  
Wide Range ◽  
Pathogen Diagnosis

Pseudomonas aeruginosa is a highly versatile environmental Gram-negative bacterium that can be isolated from a wide range of habitats, including soil, marshes, and the ocean, as well as from plant and animal tissues. It is resistant to many disinfectants and antibiotics, giving it a selective advantage in hospitals. It rarely causes infection in the healthy host but is a major opportunistic pathogen. Diagnosis is usually straightforward when the organism is cultured from samples collected from normally sterile sites, but is often challenging when infection is suspected in non-sterile sites such as a catheterized urinary tract, burns, or skin ulcers, because P. aeruginosa may be either a pathogen or an innocent bystander. Treatment can be challenging as P. aeruginosa is intrinsically resistant to a broad range of antimicrobials.

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