Cell Wall Permeability of Pinto Bean Cotyledon Cells Regulates In Vitro Fecal Fermentation and Gut Microbiota

2021 ◽  
Author(s):  
Yanrong Huang ◽  
Sushil Dhital ◽  
Feitong Liu ◽  
Xiong Fu ◽  
Qiang Huang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Structural Changes ◽  
Microbiota Composition ◽  
Colonic Fermentation ◽  
Pinto Bean ◽  
Wall Permeability ◽  
Cell Wall Permeability ◽  
Cotyledon Cells ◽  
Whole Foods

Processing induced structural changes of whole foods on regulation of colonic fermentation rate and microbiota composition are least understood and often overlooked. In the present study, intact cotyledon cells from...

scholarly journals Process-induced cell wall permeability modulates the in vitro starch digestion kinetics of common bean cotyledon cells

2018 ◽  
Vol 9 (12) ◽  
pp. 6544-6554 ◽  
Author(s):  
Andrea Pallares Pallares ◽  
Beatriz Alvarez Miranda ◽  
Ngoc Quynh Anh Truong ◽  
Clare Kyomugasho ◽  
Claire Maria Chigwedere ◽  
...  
Keyword(s):  
Cell Wall ◽  
Common Bean ◽  
Thermal Processing ◽  
Simulated Digestion ◽  
Wall Permeability ◽  
Digestion Kinetics ◽  
Cell Wall Permeability ◽  
Cotyledon Cells ◽  
Kinetics Of

Cell wall barrier role during in vitro simulated digestion of starch in common bean cotyledon cells can be modified through variation of thermal processing intensity.

An extensin peroxidase is associated with white-light inhibition of lupin (Lupinus albus) hypocotyl growth

1999 ◽  
Vol 26 (1) ◽  
pp. 29 ◽  
Author(s):  
P. Jackson ◽  
S. Paulo ◽  
C. P. P. Ricardo ◽  
M. Brownleader ◽  
P. O. Freire
Keyword(s):  
Cell Wall ◽  
White Light ◽  
Cell Expansion ◽  
Structural Changes ◽  
Lupinus Albus ◽  
Extension Rate ◽  
Hypocotyl Growth ◽  
Cross Links ◽  
Quantitative Changes

The spatial distribution of the major basic (B2; pI 8.8) peroxidase of the intercellular fluid has an inverse relation with extension rate in etiolated hypocotyls of Lupinus albus L., suggesting its possible role in the control of cell expansion. White-light irradiation of etiolated hypocotyls resulted in growth inhibition and the induction of B2 and acidic (A2, pI 4.7–5.2) isoperoxidases (EC 1.1.11.7) to higher physiological activities. However, only the activities of the B2 isoperoxidases underwent quantitative changes in both space and time which suggested their role in growth-retardation. We have purified the B2 and A2 (pI 5.2) peroxidases to apparent electrophoretic homogeneity. To corroborate evidence obtained elsewhere that growth cessation coincides with cell wall structural changes and cell wall rigidification, we have shown that the B2 peroxidase, and not A2 peroxidase, cross-links tomato extensin in vitro. The B2 peroxidase may therefore catalyse the developmentally and light regulated formation of a covalently cross-linked cell wall extensin matrix in lupin hypocotyls. The cell wall would be more rigid or more recalcitrant to wall-loosening and subsequently contribute to the control of cell expansion.

scholarly journals Carbon Source-Induced Modifications in the Mycolic Acid Content and Cell Wall Permeability of Rhodococcus erythropolis E1

2003 ◽  
Vol 69 (12) ◽  
pp. 7019-7027 ◽  
Author(s):  
Ivana Sokolovská ◽  
Raoul Rozenberg ◽  
Christophe Riez ◽  
Paul G. Rouxhet ◽  
Spiros N. Agathos ◽  
...  
Keyword(s):  
Cell Wall ◽  
Carbon Source ◽  
Acid Content ◽  
Rhodococcus Erythropolis ◽  
Mycolic Acid ◽  
Mycolic Acids ◽  
Carbon Chains ◽  
Linear Alkanes ◽  
Wall Permeability ◽  
Cell Wall Permeability

ABSTRACT The influence of the carbon source on cell wall properties was analyzed in an efficient alkane-degrading strain of Rhodococcus erythropolis (strain E1), with particular focus on the mycolic acid content. A clear correlation was observed between the carbon source and the mycolic acid profiles as estimated by high-performance liquid chromatography and mass spectrometry. Two types of mycolic acid patterns were observed after growth either on saturated linear alkanes or on short-chain alkanoates. One type of pattern was characterized by the lack of odd-numbered carbon chains and resulted from growth on linear alkanes with even numbers of carbon atoms. The second type of pattern was characterized by mycolic acids with both even- and odd-numbered carbon chains and resulted from growth on compounds with odd-numbered carbon chains, on branched alkanes, or on mixtures of different compounds. Cellular short-chain fatty acids were twice as abundant during growth on a branched alkane (pristane) as during growth on acetate, while equal amounts of mycolic acids were found under both conditions. More hydrocarbon-like compounds and less polysaccharide were exposed at the cell wall surface during growth on alkanes. Whatever the substrate, the cells had the same affinity for aqueous-nonaqueous solvent interfaces. By contrast, bacteria displayed completely opposite susceptibilities to hydrophilic and hydrophobic antibiotics and were found to be strongly stained by hydrophobic dyes after growth on pristane but not after growth on acetate. Taken together, these data show that the cell wall composition of R. erythropolis E1 is influenced by the nutritional regimen and that the most marked effect is a radical change in cell wall permeability.

scholarly journals A Tetrameric Porin Limits the Cell Wall Permeability ofMycobacterium smegmatis

2002 ◽  
Vol 277 (40) ◽  
pp. 37567-37572 ◽  
Author(s):  
Harald Engelhardt ◽  
Christian Heinz ◽  
Michael Niederweis
Keyword(s):  
Cell Wall ◽  
Wall Permeability ◽  
Cell Wall Permeability

scholarly journals The Activity of Cell-Wall Modifying β-1,3-Glucanases in Soybean Grown in Presence of Heavy Metals

2016 ◽  
Vol 15 (2) ◽  
pp. 114-121
Author(s):  
Monika Bardáčová ◽  
Marína Maglovski ◽  
Zuzana Gregorová ◽  
Yevheniia Konotop ◽  
Miroslav Horník ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Cell Wall ◽  
Cell Walls ◽  
Regulation Mechanism ◽  
Soybean Variety ◽  
Toxic Heavy Metals ◽  
Metal Sequestration ◽  
Enzyme Isoforms ◽  
Wall Permeability ◽  
Cell Wall Permeability

AbstractCell walls represent the first barrier that can prevent the entrance of toxic heavy metals into plants. The composition and the flexibility of the cell wall are regulated by different enzymes. The ß-1,3-glucanases control the degradation of the polysaccharide callose as a flexible regulation mechanism of cell wall permeability and/or its ability to bind metals under stress conditions. The profile and activity of ß-1,3-glucanases in the presence of heavy metals, however, has rarely been studied. Here we studied these enzymes in four soybean varieties (Glycine max) grown in the presence of cadmium ions. These analyses revealed three acidic and one basic enzyme isoforms in each soybean variety, but only two of the acidic isoforms in the variety Moravians were substantially responsive to the presence of Cd2+. Since the responses of certain glucanases were detected mainly in the varieties sensitive to metal and accumulating high amounts of metals, we assume their role in the defense rather than strategic metal sequestration.

scholarly journals Impact of Methoxymycolic Acid Production by Mycobacterium bovis BCG Vaccines

2004 ◽  
Vol 72 (5) ◽  
pp. 2803-2809 ◽  
Author(s):  
Adam Belley ◽  
David Alexander ◽  
Tania Di Pietrantonio ◽  
Manon Girard ◽  
Joses Jones ◽  
...  
Keyword(s):  
Cell Wall ◽  
Mycobacterium Bovis ◽  
Acid Production ◽  
Acid Synthesis ◽  
Permeability Barrier ◽  
Mycolic Acids ◽  
Cell Wall Permeability ◽  
The Impact

ABSTRACT BCG vaccines are a family of closely related daughter strains of an attenuated isolate of Mycobacterium bovis derived by in vitro passage from 1908 to 1921. During subsequent laboratory propagation of the vaccine strain until its lyophilization in 1961, BCG Pasteur underwent at least seven further genomic mutations. The impact of these mutations on the properties of the vaccine is currently unknown. One mutation, a glycine-to-aspartic acid substitution in the mmaA3 gene, occurred between 1927 and 1931 and impairs methoxymycolic acid synthesis in BCG strains obtained from the Pasteur Institute after this period. Mycolic acids of the cell wall are classified into three functional groups (alpha-, methoxy-, and ketomycolic acids), and together these lipids form a highly specialized permeability barrier around the bacterium. To explore the impact of methoxymycolic acid production by BCG strains, we complemented the functional gene of mmaA3 into BCG Denmark and tested a number of in vitro and in vivo phenotypes. Surprisingly, restoration of methoxymycolic acids alone had no effect on cell wall permeability, resistance to antibiotics, or growth in cultured macrophages and C57BL/6 mice. Our results demonstrate that the loss of methoxymycolic acid production did not apparently affect the virulence of BCG strains.

scholarly journals 3D-QSAR and Cell Wall Permeability of Antitubercular Nitroimidazoles against Mycobacterium tuberculosis

Molecules ◽  
2013 ◽  
Vol 18 (11) ◽  
pp. 13870-13885 ◽  
Author(s):  
Sang-Ho Lee ◽  
Minsung Choi ◽  
Pilho Kim ◽  
Pyung Myung
Keyword(s):  
Cell Wall ◽  
Mycobacterium Tuberculosis ◽  
3D Qsar ◽  
Wall Permeability ◽  
Cell Wall Permeability
Sign in / Sign up

Export Citation Format

Share Document