Electroactive 3D printable poly(3,4-ethylenedioxy thiophene)-graft-poly(-caprolactone) copolymers as scaffolds for muscle cell alignment

2021 ◽  
Author(s):  
Antonio Dominguez-Alfaro ◽  
Miryam Criado ◽  
David Mecerreyes ◽  
Elena Gabirondo ◽  
Haizpea Lasa ◽  
...  
Keyword(s):  
Muscle Cell ◽  
Three Dimensional ◽  
Cell Alignment ◽  
Poly Caprolactone

The development of tailor-made polymers to build artificial three-dimensional scaffolds to repair damaged skin tissues is gaining increasing attention in the bioelectronics field. Poly (3,4-ethylene dioxythiophene) (PEDOT) is the gold...

scholarly journals Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation

Polymers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 643 ◽  
Author(s):  
Dae Geun Han ◽  
Chi Bum Ahn ◽  
Ji-Hyun Lee ◽  
Yongsung Hwang ◽  
Joo Hyun Kim ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Fiber Diameter ◽  
Electrospun Fiber ◽  
Electrospun Nanofibers ◽  
Macrophage Infiltration ◽  
Vsmc Proliferation ◽  
Vascular Scaffolds ◽  
Poly Caprolactone

Due to the morphological resemblance between the electrospun nanofibers and extracellular matrix (ECM), electrospun fibers have been widely used to fabricate scaffolds for tissue regeneration. Relationships between scaffold morphologies and cells are cell type dependent. In this study, we sought to determine an optimum electrospun fiber diameter for human vascular smooth muscle cell (VSMC) regeneration in vascular scaffolds. Scaffolds were produced using poly(caprolactone) (PCL) electrospun fiber diameters of 0.5, 0.7, 1, 2, 2.5, 5, 7 or 10 μm, and VSMC survivals, proliferations, infiltrations, and phenotypes were recorded after culturing cells on these scaffolds for one, four, seven, or 10 days. VSMC phenotypes and macrophage infiltrations into scaffolds were evaluated by implanting scaffolds subcutaneously in a mouse for seven, 14, or 28 days. We found that human VSMC survival was not dependent on the electrospun fiber diameter. In summary, increasing fiber diameter reduced VSMC proliferation, increased VSMC infiltration and increased macrophage infiltration and activation. Our results indicate that electrospun PCL fiber diameters of 7 or 10 µm are optimum in terms of VSMC infiltration and macrophage infiltration and activation, albeit at the expense of VSMC proliferation.

Engineering muscle cell alignment through 3D bioprinting

2017 ◽  
Vol 105 (9) ◽  
pp. 2582-2588 ◽  
Author(s):  
Pamela Mozetic ◽  
Sara Maria Giannitelli ◽  
Manuele Gori ◽  
Marcella Trombetta ◽  
Alberto Rainer
Keyword(s):  
Muscle Cell ◽  
3D Bioprinting ◽  
Cell Alignment

scholarly journals In vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds

2020 ◽  
Vol 24 (1) ◽  
Author(s):  
Naghmeh Abbasi ◽  
Ryan S. B. Lee ◽  
Saso Ivanovski ◽  
Robert M. Love ◽  
Stephen Hamlet
Keyword(s):  
Bone Regeneration ◽  
Size Structure ◽  
Three Dimensional ◽  
Immunohistochemical Analysis ◽  
Repair Process ◽  
Micro Computed Tomography ◽  
Calvarial Defects ◽  
Promising Solution ◽  
Poly Caprolactone

Abstract Background Biomaterial-based bone tissue engineering represents a promising solution to overcome reduced residual bone volume. It has been previously demonstrated that gradient and offset architectures of three-dimensional melt electrowritten poly-caprolactone (PCL) scaffolds could successfully direct osteoblast cells differentiation toward an osteogenic lineage, resulting in mineralization. The aim of this study was therefore to evaluate the in vivo osteoconductive capacity of PCL scaffolds with these different architectures. Methods Five different calcium phosphate (CaP) coated melt electrowritten PCL pore sized scaffolds: 250 μm and 500 μm, 500 μm with 50% fibre offset (offset.50.50), tri layer gradient 250–500-750 μm (grad.250top) and 750–500-250 μm (grad.750top) were implanted into rodent critical-sized calvarial defects. Empty defects were used as a control. After 4 and 8 weeks of healing, the new bone was assessed by micro-computed tomography and immunohistochemistry. Results Significantly more newly formed bone was shown in the grad.250top scaffold 8 weeks post-implantation. Histological investigation also showed that soft tissue was replaced with newly formed bone and fully covered the grad.250top scaffold. While, the bone healing did not happen completely in the 250 μm, offset.50.50 scaffolds and blank calvaria defects following 8 weeks of implantation. Immunohistochemical analysis showed the expression of osteogenic markers was present in all scaffold groups at both time points. The mineralization marker Osteocalcin was detected with the highest intensity in the grad.250top and 500 μm scaffolds. Moreover, the expression of the endothelial markers showed that robust angiogenesis was involved in the repair process. Conclusions These results suggest that the gradient pore size structure provides superior conditions for bone regeneration.

scholarly journals Strength-duration relationship as a tool to prioritize cardiac tissue properties that govern electrical excitability

2019 ◽  
Vol 317 (1) ◽  
pp. H13-H25 ◽  
Author(s):  
Michael N. Sayegh ◽  
Natasha Fernandez ◽  
Hee Cheol Cho
Keyword(s):  
Cardiac Tissue ◽  
Three Dimensional ◽  
Assessment Tools ◽  
Electrical Strength ◽  
Design Parameters ◽  
Electrical Excitability ◽  
Cell Alignment ◽  
Tissue Properties ◽  
Duration Relationship

Gaps exist in the availability of in vitro functional assessment tools that can emulate the integration of regenerative cells and tissues to the host myocardium. We use strength-duration relationships of electrically stimulated two- and three-dimensional myocardial constructs to study the effects of pacing frequency, culture dimensions, anisotropic cell alignment, fibroblast content, and pacemaker phenotype on electrical excitability. Our study delivers electrical strength-duration as a quantifiable parameter to evaluate design parameters of engineered cardiac tissue constructs.

scholarly journals Fabrication of Nanohydroxyapatite/Poly(caprolactone) Composite Microfibers Using Electrospinning Technique for Tissue Engineering Applications

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Mohd Izzat Hassan ◽  
Tao Sun ◽  
Naznin Sultana
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
Average Diameter ◽  
Tissue Engineering Scaffold ◽  
Engineering Applications ◽  
Electrospinning Technique ◽  
Fibrous Scaffolds ◽  
Nanosized Hydroxyapatite ◽  
Poly Caprolactone

Tissue engineering fibrous scaffolds serve as three-dimensional (3D) environmental framework by mimicking the extracellular matrix (ECM) for cells to grow. Biodegradable polycaprolactone (PCL) microfibers were fabricated to mimic the ECM as a scaffold with 7.5% (w/v) and 12.5% (w/v) concentrations. Lower PCL concentration of 7.5% (w/v) resulted in microfibers with bead defects. The average diameter of fibers increased at higher voltage and the distance of tip to collector. Further investigation was performed by the incorporation of nanosized hydroxyapatite (nHA) into microfibers. The incorporation of 10% (w/w) nHA with 7.5% (w/v) PCL solution produced submicron sized beadless fibers. The microfibrous scaffolds were evaluated using various techniques. Biodegradable PCL and nHA/PCL could be promising for tissue engineering scaffold application.

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