scholarly journals Antioxidative and antiphotoaging activities of neferine upon UV-A irradiation in human dermal fibroblasts

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Abidullah khan ◽  
Hongliang Bai ◽  
Maoguo Shu ◽  
Mingxia Chen ◽  
Amin Khan ◽  
...  
Keyword(s):  
Deleterious Effect ◽  
Dermal Fibroblasts ◽  
The Other ◽  
Oxygen Species ◽  
Human Dermal Fibroblasts ◽  
Ultraviolet A ◽  
Enzymatic Antioxidant ◽  
Matrix Metalloproteinase 1 ◽  
Prevention And Treatment ◽  
Skin Photoaging

Our daily exposure to ultraviolet radiation (UVR) results in the production of reactive oxygen species (ROS), lipids, proteins and DNA damage and alteration in fibroblast structure, thus contributing to skin photoaging. For this reason, the use of natural bioactive compounds with antioxidant activity could be a strategic tool to overcome ultraviolet A (UV-A) induced deleterious effect. Neferine is an alkaloid extract from the seed embryos of lotus (Nelumbo nucifera Gaertn). In the present study, we report the protective effect of neferine against UV-A induced oxidative stress and photoaging in human dermal fibroblasts (HDFs). HDFs subjected to UV-A irradiation showed increased production of ROS and malondialdehyde (MDA). Furthermore, it depleted the cellular enzymatic antioxidant superoxide dismutase (SOD) and non-enzymatic antioxidant glutathione peroxidase (GPx). On the other hand, HDFs treated with neferine followed by UV-A irradiation reversed the process, reduced the ROS and lipid peroxidation and restored the antioxidants pool. Moreover, neferine treatment significantly inhibited UV-A induced matrix metalloproteinase-1 (MMP-1) expression in HDFs. Remarkable morphological and ultrastructural alterations observed in HDFs upon UV-A irradiation, were also reduced with neferine treatment. Taken together, our results suggest that neferine has strong antioxidative and photoprotective properties and thus may be a potential agent for the prevention and treatment of UV-A mediated skin photoaging.

scholarly journals Ultrasmall prussian blue nanoparticles attenuate UVA-induced cellular senescence in human dermal fibroblasts via inhibiting ERK/AP-1 pathway

Nanoscale ◽  
2021 ◽  
Author(s):  
Yueyue Li ◽  
Ni Zeng ◽  
Zhiguo Qin ◽  
Yihe Chen ◽  
Qian Lu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Prussian Blue ◽  
Cellular Senescence ◽  
Dermal Fibroblasts ◽  
Oxygen Species ◽  
Human Dermal Fibroblasts ◽  
Ultraviolet A ◽  
Uva Irradiation ◽  
Prussian Blue Nanoparticles ◽  
Skin Photoaging

Ultraviolet A (UVA) irradiation can induce cellular senescence and cause skin photoaging, which is mainly driven by excessive production of reactive oxygen species (ROS). Emerging studies have focused on new...

scholarly journals Concentrated Growth Factor inhibit UVA-induced photoaging in Human Dermal Fibroblasts via the MAPK/AP-1 pathway

2020 ◽  
Author(s):  
Meng Zhang ◽  
Tai Zhang ◽  
Yanan Tang ◽  
Guiyun Ren ◽  
Yanning Zhang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Uv Light ◽  
Underlying Mechanism ◽  
Protective Role ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Mitogen Activated Protein Kinase ◽  
Human Dermal Fibroblasts ◽  
Matrix Metalloproteinase 1 ◽  
Skin Photoaging

Ultraviolet (UV) radiation-induced photoaging is one of the contributors to skin aging. UV light triggers oxidative stress, producing a large number of matrix metalloproteinases and degrading the extracellular matrix in skin cells, thereby causing a series of photoaging symptoms. Concentrated growth factor (CGF) is a leukocyte- and platelet-rich fibrin biomaterial that plays a protective role in the occurrence and development of skin photoaging. In the present study, we investigated the underlying mechanism of CGF in the UVA-induced photoaging of human dermal fibroblasts (HDFs). A primary culture of HDFs was isolated from normal human facial skin. The cells were treated with CGF following UVA radiation. Proliferation of cells was detected using MTT assay, followed by measurement of reactive oxygen species (ROS) using immunofluorescence assay and flow cytometry. The mRNA and protein expression levels of P38, c-Jun, and matrix metalloproteinase-1 were detected using real-time polymerase chain reaction and western blot, respectively. CGF was found to improve cell viability by inhibiting the production of ROS and reducing oxidative damage. In addition, there was lower expression of p38 and c-Jun at the mRNA and protein levels following CGF treatment, thus resulting in the inhibition of MMP-1 expression. Our results suggest that CGF could protect HDFs against UVA-induced photoaging by blocking the P38 mitogen-activated protein kinase/activated protein-1 (P38MAPK/AP-1) signaling pathway. These findings provide a new clinical strategy for the prevention of skin photoaging.

scholarly journals Efficacy of Alpinumisoflavone Isolated from Maclura tricuspidata Fruit in Tumor Necrosis Factor-α-Induced Damage of Human Dermal Fibroblasts

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 514
Author(s):  
Sullim Lee ◽  
Giang Do Hoang ◽  
Daeyoung Kim ◽  
Ho Sueb Song ◽  
Sungyoul Choi ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Dermal Fibroblasts ◽  
The Body ◽  
Hazardous Substances ◽  
Human Dermal Fibroblasts ◽  
Cutaneous Lesions ◽  
Matrix Metalloproteinase 1 ◽  
Tnf Α ◽  
Necrosis Factor

The skin is an important organ in the human body that protects the body from environmentally hazardous substances. Reactive oxygen species (ROS) cause inflammatory reactions and degradation of the extracellular matrix leading to skin aging and various cutaneous lesions. This study evaluated the potential of isoflavones isolated from Maclura tricuspidata fruit to prevent TNF-α-induced skin inflammation in normal human dermal fibroblasts (HDFs). It focused on alpinumisoflavone (AIF) that suppressed the accumulation of ROS and nitric oxide (NO) in tumor necrosis factor-alpha (TNF-α)-treated HDFs. AIF inhibited the TNF-α-induced increase in matrix metalloproteinase-1, decreased procollagen I α1, and suppressed pro-inflammatory mediators and pro-inflammatory cytokines, including NO synthase, cyclooxygenase-2, interleukin (IL)-1β, IL-6, and IL-8 that trigger inflammatory responses. AIF inhibited nuclear factor-κB and activating protein 1 mitogen-activated protein kinases that were increased by TNF-α stimulation. These results suggest that AIF may protect skin from aging and various cutaneous lesions.

scholarly journals Galvanic microparticles increase migration of human dermal fibroblasts in a wound-healing model via reactive oxygen species pathway

2014 ◽  
Vol 320 (1) ◽  
pp. 79-91 ◽  
Author(s):  
Nina Tandon ◽  
Elisa Cimetta ◽  
Aranzazu Villasante ◽  
Nicolette Kupferstein ◽  
Michael D. Southall ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Wound Healing ◽  
Reactive Oxygen ◽  
Dermal Fibroblasts ◽  
Oxygen Species ◽  
Human Dermal Fibroblasts ◽  
Wound Healing Model ◽  
Healing Model
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