Effect of new bedding material on morpho-biochemical and immunological factors of gosling blood

The purpose of research and production experiment in the conditions of Akhmetov farm located in the Vysokogorsky district of the Republic of Tatarstan was to study the harmlessness of new sanitary bedding material and the effect on gosling broilers. The experiment evidenced that a new sanitary bedding material made of woodworking waste, thermochemically and biotechnologically treated, does not have an irritating effect on the skin and mucous membranes of the eye, nor does it provoke allergic reactions in rabbits, but, on the contrary, improves certain morphobiochemical and immunological factors of gosling blood. Ultimately, this resulted in accelerated red blood cells by 6.94 %, hemoglobin – 5.20 %, total protein – 6.30 %, γ-globulin – 9.38 %, total calcium – 6.14 %, inorganic phosphorus – 5.31 %, glucose – 5.38 %, increased phagocytic activity by 2.24 %, phagocytic number – 4.37 %, and increased number of S. Marcescens-stimulated cells through the NBT test by 5.17 % compared with the control, respectively. The data obtained are consistent with the advanced livestock viability in the experiment by 1.8 %, as compared with the control.

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THE INFLUENCE OF NEW BEDDING MATERIAL ON MORPHOBIOCHEMICAL AND IMMUNOLOGICAL PARAMETERS OF BLOOD OF GOSLINGS OF BROILERS

Scientific Notes Kazan Bauman State Academy of Veterinary Medicine ◽

10.31588/2413-4201-1883-247-3-244-248 ◽

2021 ◽

Vol 247 (3) ◽

pp. 244-248

Author(s):

V.G. Sofronov ◽

◽

R.N. Fayzrakhmanov ◽

N.I. Danilova ◽

E.L. Kuznetsova ◽

...

Keyword(s):

Red Blood Cells ◽

Total Protein ◽

Blood Cells ◽

Inorganic Phosphorus ◽

The Body ◽

Allergic Reactions ◽

Bedding Material ◽

Total Calcium ◽

Immunological Parameters ◽

Mucous Membranes

The harmlessness of the new hygienic bedding material and the effect of broilers on the body have been studied. During the experiment, it was found that the new hygienic litter material, representing waste from the woodworking industry, treated with thermochemical and biotechnological methods, does not have an irritating effect on the skin, does not irritate the mucous membranes of the eyes and allergic reactions in rabbits, but, on the contrary, improves certain morphobiochemical and immunological parameters of the body’s blood are goslings. Ultimately, this was reflected in an increase in red blood cells by 7.04 %, hemoglobin – 5.20 %, total protein – 6.3 %, total calcium – 5.8 %, inorganic phosphorus – 5.1 %, glucose – 5,4 % compared with the control.

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Effect of feeding chelates of trace elements on morphological and biochemical blood indicators of cows

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.15421/nvlvet7426 ◽

2017 ◽

Vol 19 (74) ◽

pp. 119-122

Author(s):

S. V. Kulibaba ◽

M. M. Dolgaya ◽

N. S. Emelyanova ◽

G. O. Goncharenko

Keyword(s):

Trace Elements ◽

Total Protein ◽

Blood Cells ◽

Inorganic Phosphorus ◽

Total Calcium ◽

Chelate Complexes ◽

Animal Blood ◽

Significant Difference ◽

Experimental Group ◽

Effect Of Feeding

In the article was discusses the questions about the effect of feeding chelate complexes of trace elements of copper (Cu), zinc (Zn), manganese (Mn) on morphological and biochemical blood indicators of cows of Ukrainian black mottled dairy breed on the second month of lactation. For the experiment, 40 cows were selected and formed four groups: one control and three experimental. The cows in the control group fed a premix of sulfate salts of Cu, Zn and Mn in doses that are 100% covered their lack in a forage. Experimental animals from I, II and III groups were fed premixes with chelate complexes of Cu, Zn and Mn, which offset the deficiency of trace elements in the feed ration for 100, 50 and 25% (calculated on pure element), respectively. In animal blood examined the number of red blood cells, white blood cells, hemoglobin, phagocytic activity of neutrophils, and in serum – total protein, albumin, globulin, glucose, total calcium, inorganic phosphorus, carotene, creatinine, urea, cholesterol, activity of AST, ALT and alkaline phosphatase. As a result studies, have shown that blood indicators of the animals were within physiological fluctuations. Found that in the serum of animals of III experimental group the contents of total protein was lowered by 4.1% (P < 0.05) than in the first, and by 3.6% (P < 0.1) relative to the second group. It was determined that the concentration of albumin and total calcium in serum of II experimental group of cows was higher by 4.6% and by 6.0% (P < 0.1), respectively, relative to the third group. Content of inorganic phosphorus in the blood serum of animals of II group was higher at trends over the indicator in the serum of animals of the control and first experimental groups by 8.1% and by 6.9%, respectively. According to other indicators of animals blood does not have significant difference.

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Stable Mixed Hematopoietic Chimerism in Dogs Conditioned with Donor Antigen, Anti-CD154 Antibody and 100cGy TBI.

Blood ◽

10.1182/blood.v106.11.5210.5210 ◽

2005 ◽

Vol 106 (11) ◽

pp. 5210-5210

Author(s):

Christoph Jochum ◽

Mechthild Beste ◽

Eustacia Zellmer ◽

Michael A. Harkey ◽

Scott S. Graves ◽

...

Keyword(s):

Blood Cells ◽

White Blood Cells ◽

Sublethal Dose ◽

Mixed Chimerism ◽

Allergic Reactions ◽

Donor Cells ◽

Mucous Membranes ◽

Hematopoietic Chimerism ◽

Total Body ◽

Peripheral White Blood Cells

Abstract Stable mixed chimerism can be established in dogs given a sublethal dose of 200cGy total body irradiation (TBI) before and immunosupression with mycophenolate mofetil (MMF) and Cyclosporine (CSP). When the TBI dose is reduced to 100 cGy, only transient engraftment is observed. Here we asked whether stable engraftment after 100 cGy TBI could be accomplished by first reducing the intensity of host immune responsiveness with the help of an anti-CD154 antibody which blocks the CD40-CD154 pathway. Accordingly, recipients were given a single i.v. injection of 5 mg/kg anti-CD154 antibody (day -5) followed one day later by combined i.v./sc. injections of 107 peripheral white blood cells/kg from their intended DLA-identical littermate marrow donors. TBI, 100cGy (delivered at 7 cGy/min), was given on day 0 followed by infusion of a median of 4.22x108 marrow cells/kg. Postgrafting immunosuppression consisted of MMF (10mg/kg, BID, days 0 to 28) and CSP (15 mg/kg BID, days -1 to 35). Six dogs have been transplanted. Two dogs are too early after transplant for evaluation, while four dogs have been followed for >9, 12, >18 and >26 weeks. All four dogs had initial engraftment. Three of four dogs have continued to show stable mixed donor/host hematopoietic chimerism among lymphocytes and granulocytes from the peripheral blood. One dog rejected the graft 12 weeks after transplantation. All six transplanted dogs are alive and well. Besides mild allergic reactions to the antibody in five of the six dogs (redness of the eyes, skin rashes and dry mucous membranes), no additional side effects attributed to the pretransplant treatment were observed. Data were consistent with previous findings in dogs, in which the costimulatory signal between B7 and CD28 was blocked, and support the hypothesis, that stable marrow allografts would be established by combining non-myeloablative pretransplant host immunosuppression and posttransplant immunosupression of both host and donor cells using MMF and CSP.

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CHANGES OF ORGANIC ACID-SOLUBLE PHOSPHORUS, DIPHOSPHOGLYCERATE, ADENOSINETRIPHOSPHATE, AND INORGANIC PHOSPHORUS IN THE BLOOD CELLS OF RATS DURING THE DEVELOPMENT AND HEALING OF RICKETS

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)73882-9 ◽

1938 ◽

Vol 126 (2) ◽

pp. 749-761

Author(s):

S. Rapoport ◽

George Martin Guest

Keyword(s):

Organic Acid ◽

Blood Cells ◽

Inorganic Phosphorus ◽

Soluble Phosphorus

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Improving transfusion practice in transfusion dependent thalassaemia patients

Thalassemia Reports ◽

10.4081/thal.2017.6821 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 1

Author(s):

Chathupa Wickremaarachchi ◽

Elizabeth McGill ◽

Annmarie Bosco ◽

Giselee Kidson-Gerber

Keyword(s):

Blood Cells ◽

Maximum Rate ◽

Fluid Overload ◽

Transfusion Practice ◽

Red Cells ◽

Transfusion Rate ◽

Allergic Reactions ◽

Medical Review ◽

Transfusion Reaction ◽

Change In Practice

The aim of this study was to improve current transfusion practice in transfusiondependent thalassaemia patients by determining whether safe transition from triplewashed red cells (TWRC) to leucodepleted red cells (LDRC), increasing transfusion rates, reducing the use of frusemide and creating uniform practice across patients is possible. In patients receiving regular transfusions (50), triple-washed red blood cells were changed to LDRC, transfusion rates were increased to 5 mL/kg/h (in line with the Cooley’s Foundation guidelines) to a maximum of 300 mL/h and frusemide was ceased. Medical review occurred at completion of the transfusion. Of the 20 patients on TWRC, 18 were transitioned to leucodepleted red cells (90%). Recurrent allergic reactions in 2 patients required re-institution of TWRC. 7 of the 8 patients on regular frusemide ceased this practice with no documented transfusion-related fluid overload. One patient refused. Of the eligible 50 patients, 20 patients (40%) were increased to the maximum transfusion rate of 300 mLs/h; 6 (12%) increased rate but refused to go to the maximum; 9 (18%) refused a change in practice and 15 (30%) were already at the maximum rate. There was only one documented transfusion reaction (palpitations) however this patient was able to tolerate a higher transfusion rate on subsequent transfusions. Thalassemia patients on TWRC were safely transitioned to LDRC. Transfusion rates were safely increased, with a calculated reduction in day-stay bed time of 17.45 h per month. This confirms a guideline of 5 mL/kg/h for transfusion-dependant thalassaemia patients with preserved cardiac function is well tolerated and may be translated to other centres worldwide. 本研究的目的是通过确定是否有可能进行从三洗红细胞（TWRC）到去白细胞红细胞（LDRC）的安全过渡，提高输血速率，减少速尿的使用，并在患者中实施统一规则，从而改进输血依赖型地中海贫血患者中现有的输血实践。在接受定期输血的患者（50例）中，将三洗红细胞改为 LDRC，输血速率提高至5 mL/kg/h（符合库利氏贫血基金会的指引），最高可达到300 mL/h，并停止使用速尿。输血完成后进行体检。在使用TWRC的20例患者中，18例转为去白细胞红细胞（90%）。2例患者产生的复发性过敏反应需要重新输以TWRC。8名定期使用速尿的患者中，7名中止了使用该药物，并且没有输血相关液体超负荷的相关记录。一名病人拒绝。在符合条件的50例患者中，20例（40%）增加至300 mL/h的最大输血速率；6例（12%）输血速率提高但拒绝增加至最大；9例（18%）拒绝做出改变，15例（30%）已经达到了最大速率。只产生一例有记录的输血反应（心悸），但是该患者在随后的输血中能够耐受更高的输血速率。使用TWRC的地中海贫血患者安全转用LDRC。输血速率安全地得到提高，计算出减少的白天卧床时间为每月17.45小时。这证实了5 mL/kg/h的指引在心功能得到保护的输血依赖型地中海贫血患者中有良好的耐受性，可以推广至全球其他中心。

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Метаболічний профіль крові корів, хворих на післяродову гіпокальціємію

Вісник Полтавської державної аграрної академії ◽

10.31210/visnyk2013.02.20 ◽

2013 ◽

pp. 76-80

Author(s):

Л. Л. Юськів ◽

В. В. Влізло

Keyword(s):

Fatty Acids ◽

Alkaline Phosphatase ◽

Parathyroid Hormone ◽

Total Protein ◽

Clinical Signs ◽

Inorganic Phosphorus ◽

Total Calcium ◽

25 Hydroxycholecalciferol

Проведено дослідження вмісту 25-гідроксихолекальциферолу (25-ОНD3), паратиреоїдного гормону (ПТГ) та кальцитоніну (КТ), а також кальцію загального, зв'язаного з білком та ультрафільтрувального, неорганічного фосфору, маґнію, активності лужної фосфатази, неетерифікованих жирних кислот (НЕЖК), глюкози і білка у крові корів, хворих на післяродову гіпокальціємію. Встановлено, що у крові корів із клінічними ознаками післяродової гіпокальціємії вміст 25-ОНD3 був вищим, а вміст ПТГ і КТ – нижчим, порівняно зі здоровими коровами в 1–2-й день після отелення. Водночас у крові корів, хворих на післяродову гіпо-кальціємію, знижується вміст загального, білокзв’язаного та ультрафільтрувального кальцію, неорганічного фосфору, глюкози, загального білка й підвищується кількість НЕЖК, маґнію, а також активність лужної фосфатази. It was researched the content of 25-hydroxycholecalciferol (25-OND3), parathyroid hormone (PTH) and calcitonin (CT) and total calcium bounded with the protein and ultrafiltered inorganic phosphorus, magnesium, alkaline phosphatase, nonetherified fatty acids (NEFA), glucose and protein in the blood of cows suffering from postpartum hypocalcemia. It was found that in the blood of cows with clinical signs of postpartum hypocalcemia ,25- OHD3 content was higher, but the content of PTH and CT - lower in coparisant with healthy cows within 1-2 days after calving. However, in the blood of cows suffering from postpartum hypocalcemia was decreasing concentration of total, protein-linked and ultrafiltrates calcium, inorganic phosphorus, glucose, total protein and was increasing concentration of NEFA, magnesium and activity of alkaline phosphatase.

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Serum proteinogram and biochemistry of Holstein cows in the peripartum period

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-5819 ◽

2019 ◽

Vol 39 (5) ◽

pp. 342-347

Author(s):

Thaís G. Rocha ◽

Camila Bortoletto ◽

Daniela G. Silva ◽

Kalina M.M.G. Simplício ◽

Luiz F. Zafalon ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Dairy Cows ◽

Total Protein ◽

Repeated Measures ◽

Transition Period ◽

Serum Levels ◽

Inorganic Phosphorus ◽

Holstein Cows ◽

Total Calcium ◽

Peripartum Period

ABSTRACT: In face of the few reports found in national literature analyzing the potential influence of parturition number in serum proteinogram and biochemical profile in the peripartum period of high yielding dairy cows, the aim of the present study was to comparatively evaluate the dynamics of these serum constituents’ concentrations in blood samples obtained from primiparous and multiparous Holstein cows, 60 and 30 days prepartum and in the day of parturition. Data were analyzed by repeated measures variance analysis (ANOVA) and differences between groups and moments were analyzed by Tukey’s test. Results were considered significant when P<0.05. Parity influenced levels of total protein, albumin, globulins, magnesium, cholesterol, which were higher in multiparous cows, as well as concentrations of ceruloplasmin, total calcium, chloride and alkaline phosphatase activity, which were higher in primiparous cows. Parturition influenced serum concentrations of ceruloplasmin (+58%), transferrin (-25%), haptoglobin (+33%), total protein (-17%), globulins (-25%), immunoglobulin A (-43%), immunoglobulin G (-24%), total calcium (-12%), inorganic phosphorus (-10%), chloride (+5%), sodium (+4%), cholesterol (-23%), triglycerides (-38.6%), as well as activities of aspartate aminotransferase (+14%) and alkaline phosphatase (+28%). A decrease in serum levels of total calcium, inorganic phosphorus, cholesterol and triglycerides was more pronounced in multiparous than in primiparous cows. These results demonstrate that the interpretation of proteinogram and serum constituents should take into consideration lactation number and the moment of parturition as relevant factors in high yielding dairy cows in the transition period.

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Determination of haematological and biochemical parameters of Przewalski horses (Equus przewalski) kept by the Prague Zoo

Veterinární Medicína ◽

10.17221/7242-vetmed ◽

2014 ◽

Vol 59 (No. 1) ◽

pp. 11-21 ◽

Cited By ~ 6

Author(s):

J. Tomenendalova ◽

R. Vodicka ◽

I. Uhrikova ◽

J. Doubek

Keyword(s):

Blood Cells ◽

Biochemical Parameters ◽

Total Bilirubin ◽

Age Groups ◽

Inorganic Phosphorus ◽

The Czech Republic ◽

Eosinophilic Granulocytes ◽

Domestic Horses ◽

Lower Count

The objective of this study was to determine ranges of haematological and biochemical parameters of Przewalski horses (Equus przewalski) kept in the Czech Republic, to compare the results with data reported for the same species as well as for domestic horses (Equus caballus), and to evaluate differences between both genders and age groups. Stallions showed significantly higher counts of red blood cells and bands, higher levels of total bilirubin and magnesium, and a higher activity of GGT. Higher concentrations of cholesterol, vitamin A and sodium were found in mares. Young animals were characterised by a lower count of eosinophilic granulocytes, a higher activity of ALP and higher concentrations of calcium, inorganic phosphorus, sodium and creatinine. Other differences were not significant.  

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