scholarly journals Effect of feeding chelates of trace elements on morphological and biochemical blood indicators of cows

2017 ◽  
Vol 19 (74) ◽  
pp. 119-122
Author(s):  
S. V. Kulibaba ◽  
M. M. Dolgaya ◽  
N. S. Emelyanova ◽  
G. O. Goncharenko
Keyword(s):  
Trace Elements ◽  
Total Protein ◽  
Blood Cells ◽  
Inorganic Phosphorus ◽  
Total Calcium ◽  
Chelate Complexes ◽  
Animal Blood ◽  
Significant Difference ◽  
Experimental Group ◽  
Effect Of Feeding

In the article was discusses the questions about the effect of feeding chelate complexes of trace elements of copper (Cu), zinc (Zn), manganese (Mn) on morphological and biochemical blood indicators of cows of Ukrainian black mottled dairy breed on the second month of lactation. For the experiment, 40 cows were selected and formed four groups: one control and three experimental. The cows in the control group fed a premix of sulfate salts of Cu, Zn and Mn in doses that are 100% covered their lack in a forage. Experimental animals from I, II and III groups were fed premixes with chelate complexes of Cu, Zn and Mn, which offset the deficiency of trace elements in the feed ration for 100, 50 and 25% (calculated on pure element), respectively. In animal blood examined the number of red blood cells, white blood cells, hemoglobin, phagocytic activity of neutrophils, and in serum – total protein, albumin, globulin, glucose, total calcium, inorganic phosphorus, carotene, creatinine, urea, cholesterol, activity of AST, ALT and alkaline phosphatase. As a result studies, have shown that blood indicators of the animals were within physiological fluctuations. Found that in the serum of animals of III experimental group the contents of total protein was lowered by 4.1% (P < 0.05) than in the first, and by 3.6% (P < 0.1) relative to the second group. It was determined that the concentration of albumin and total calcium in serum of II experimental group of cows was higher by 4.6% and by 6.0% (P < 0.1), respectively, relative to the third group. Content of inorganic phosphorus in the blood serum of animals of II group was higher at trends over the indicator in the serum of animals of the control and first experimental groups by 8.1% and by 6.9%, respectively. According to other indicators of animals blood does not have significant difference.

scholarly journals THE INFLUENCE OF NEW BEDDING MATERIAL ON MORPHOBIOCHEMICAL AND IMMUNOLOGICAL PARAMETERS OF BLOOD OF GOSLINGS OF BROILERS

2021 ◽  
Vol 247 (3) ◽  
pp. 244-248
Author(s):  
V.G. Sofronov ◽  
◽  
R.N. Fayzrakhmanov ◽  
N.I. Danilova ◽  
E.L. Kuznetsova ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Inorganic Phosphorus ◽  
The Body ◽  
Allergic Reactions ◽  
Bedding Material ◽  
Total Calcium ◽  
Immunological Parameters ◽  
Mucous Membranes

The harmlessness of the new hygienic bedding material and the effect of broilers on the body have been studied. During the experiment, it was found that the new hygienic litter material, representing waste from the woodworking industry, treated with thermochemical and biotechnological methods, does not have an irritating effect on the skin, does not irritate the mucous membranes of the eyes and allergic reactions in rabbits, but, on the contrary, improves certain morphobiochemical and immunological parameters of the body’s blood are goslings. Ultimately, this was reflected in an increase in red blood cells by 7.04 %, hemoglobin – 5.20 %, total protein – 6.3 %, total calcium – 5.8 %, inorganic phosphorus – 5.1 %, glucose – 5,4 % compared with the control.

scholarly journals State of protein and mineral exchange in broiler-chickens for the use of Zinc and Mangane chelates

2019 ◽  
pp. 85-94
Author(s):  
V. Sakara ◽  
A. Melnyk ◽  
F. Markhenkov
Keyword(s):  
Total Protein ◽  
Broiler Chickens ◽  
Zinc Content ◽  
Calcium Content ◽  
Inorganic Phosphorus ◽  
The Body ◽  
Control Group ◽  
Total Protein Content ◽  
Total Calcium ◽  
Experimental Group

The results of the application of a complex of Zinc and Manganese chelates to protein, macro- and micro-mineral exchanges in the body of broiler chickens of the Cobb 500 cross-breed are presented. The administration of Zn and Mn chelates in a dose of 0,2 ml/l for chicken broilers of 23 days of age (1st experimental group) during 14 days influenced the increase in the total protein content to 35,3±0,8 g/l, which is 8 % higher compared with the control – 32,5±0,7 g/l (p<0,01 ) The concentration of total Calcium in serum of broiler chickens 37-day-old (end of trial) was higher in the first experimental group by 7,3 % (2,05±0,06 mmol/l) compared to control – 1,9±0,03 mmol/l. In the second group, where chelates were administered in a dose of 0,4 ml/l of water, the Calcium content was 2,0±0,02 mmol/l, which is 5 % more than the control group. The two-week application of Zn and Mn chelates also led to an increase in the level of inorganic Phosphorus in the serum of the first group to 2,6±0,12 mmol/l (+ 11,5 %) compared with the control group. After administration of chelates Zn and Mn at doses of 0,2 ml/l water, the Zinc content was higher by 4,3% (23,5±0,2 μmol/l) compared to control – 22,5±0,34 μmol/l (p<0,01). In the second group, where the dose of chelates was 0,4 ml/l, the concentration of Zinc was 23,6±0,16 μmol/l, which is more than 4,6 % for control (p<0,01). At the end of the study, the content of Manganese in serum of broiler chickens in the first group was 1,9±0,07 μmol/l (p<0,05), which is 10,4% higher compared with the control – 1,7±0,06 μmol/l (3rd sampling). The level of this element in the second group was 2,0±0,08 μmol/l (p<0,01), which is higher by 16,7 % relative to the control. At the end of the experiment, the coupon concentration in the first and second experimental groups was 12,6 and 9,3 % higher, compared with control, 7,0±0,37 μmol/l. In turn, the serum Ferrum content of the first group was greater by 9,3 % (19,3±0,69 μmol/l), and the second by 6,7 % (18,8±0,47 μmol/l) compared to the bird of the control group. Key words: chicken broilers, chelates, Zinc, Manganese, Cuprom, Ferrum, metabolism.

scholarly journals Застосування неорганічних та органічних сполук Со, Cu та Zn за їх недостатності у дійних корів

2017 ◽  
Vol 19 (78) ◽  
pp. 177-181
Author(s):  
L.G. Slivinska ◽  
V.I. Rusyn ◽  
I.A. Maksymovych ◽  
M.I. Leno ◽  
B.O. Chernushkin ◽  
...  
Keyword(s):  
Trace Elements ◽  
Body Weight ◽  
Dairy Cows ◽  
Organic Compounds ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Total Calcium ◽  
Ratio Of Components ◽  
Experimental Group ◽  
Cu And Zn

The results of research of certain biochemical parameters of blood in dairy cows for the prevention of insufficiency of Co, Cu and Zn by inorganic and organic compounds of trace elements are presented. The purpose of the work – to investigate the state of individual biochemical parameters of blood in dairy cows when using inorganic and organic compounds Co, Cu and Zn to prevent their insufficiency. The research material was the blood serum of experimental animals, which determined the content of total protein and the percentage of albumin, total calcium content, inorganic phosphorus, aspartateaminotransferase (AST) activity, alanineaminotransferase (ALT), alkaline phosphatase (AP).For research on dairy cows of the first experimental group, together with the feeds of the main diet, a mixture of trace elements sulphates was fed in the following ratio of components (mg/100 kg body weight): CuSO4 – 50, CoSO4 – 5, ZnSO4 – 120. Animals of the second experimental group fed a mixture of methionates and lysinates of trace elements in the following ratio of components (mg/ 100 kg of body weight): CuMet – 15, CuLis – 15, CoMet – 1, CoLis – 1, ZnMet – 35, ZnLis – 35. Feeding the mixture of compounds of fragile microelements was carried out by mixing them with mixed fodder, once a day, for 60 days. The evaluation of the research results was carried out at the beginning and at the end of the experiment.On the background of the deficit of Сobalt, Сuprum and Zinc, clinically diseased animals have hypoproteinemia, hypoalbuminemia, hypocalcemia, increased activity of AST, ALT and AP, indicating a metabolic abnormality in cows and development of microelementosis. It was established that the use of chelated cheeses in the form of methionates and lysinates of scarce microelements had a better therapeutic effect compared to inorganic compounds. The result of the application of chelate compounds of trace elements was a possible increase in the content of total protein in the blood serum, fractions of albumins, total calcium, decrease in the activity of AST, ALT and AP. 

scholarly journals BIOLOGICAL EFFECTS OF SILICON OXIDE NANOPARTICLES ON BROILER CHICKEN

2020 ◽  
Vol 5 (2) ◽  
pp. 63-71
Author(s):  
Vladimir Nikulin ◽  
Aleksandra Mustafina
Keyword(s):  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Silicon Oxide ◽  
The Body ◽  
Control Group ◽  
The Third ◽  
Experimental Group

The aim of the study is to increase the productive qualities of broiler chickens by including ultrafine silicon oxide into main diet. During the experiment, the biological effect of ultrafine silicon oxide on broiler chickens was estab-lished. Use of ultrafine SiO2 particles for poultry feeding contributed to an increase in the number of red blood cells and content of total protein and albumins. By the end of the experiment, the number of red blood cells in birds in-creased by 17.43% (P≤0.001) – in the blood of birds of the first experimental group, 16.51% (P≤0.01) – the second one, 20.80% (P≤0.001) – the third experimental and 21.71% (P≤0.001) – the fourth experimental group, compared with the indicator of the control group. The amount of total protein in blood serum of chickens of the first and the second experimental groups increased by 1.36-1.39 %, in the third and fourth ones there was a significant (P≤0.05) increase by 5.45 and 3.05%, respectively. The blood glucose content of chickens in the experimental groups is higher by 8.04-23.65% compared to this indicator with ones in the control group. During the experiment feed con-sumption per 1 kg gain of live weight decreased: in the first experimental group by 3.00 % in the second by – 0.50 %, the third – 6.00 % the fourth– by 4.50 %, compared to this with the control group. The chicken’s vibrancy of the first the experimental group was higher by 4.77 %, the second – by 6.20 %, the third – by 19.25 % and the fourth– by 11.59% than in the control one. Consequently, when converting the feed energy into the body energy of a broiler chicken, the energy conversion coefficient of the experimental group of poultry is higher than that of the control one by 7.16-21.76 %. Thus, the most optimal dose for further research was determined.

scholarly journals Метаболічний профіль крові корів, хворих на післяродову гіпокальціємію

2013 ◽  
pp. 76-80
Author(s):  
Л. Л. Юськів ◽  
В. В. Влізло
Keyword(s):  
Fatty Acids ◽  
Alkaline Phosphatase ◽  
Parathyroid Hormone ◽  
Total Protein ◽  
Clinical Signs ◽  
Inorganic Phosphorus ◽  
Total Calcium ◽  
25 Hydroxycholecalciferol

Проведено дослідження вмісту 25-гідроксихолекальциферолу (25-ОНD3), паратиреоїдного гормону (ПТГ) та кальцитоніну (КТ), а також кальцію загального, зв'язаного з білком та ультрафільтрувального, неорганічного фосфору, маґнію, активності лужної фосфатази, неетерифікованих жирних кислот (НЕЖК), глюкози і білка у крові корів, хворих на післяродову гіпокальціємію. Встановлено, що у крові корів із клінічними ознаками післяродової гіпокальціємії вміст 25-ОНD3 був вищим, а вміст ПТГ і КТ – нижчим, порівняно зі здоровими коровами в 1–2-й день після отелення. Водночас у крові корів, хворих на післяродову гіпо-кальціємію, знижується вміст загального, білокзв’язаного та ультрафільтрувального кальцію, неорганічного фосфору, глюкози, загального білка й підвищується кількість НЕЖК, маґнію, а також активність лужної фосфатази. It was researched the content of 25-hydroxycholecalciferol (25-OND3), parathyroid hormone (PTH) and calcitonin (CT) and total calcium bounded with the protein and ultrafiltered inorganic phosphorus, magnesium, alkaline phosphatase, nonetherified fatty acids (NEFA), glucose and protein in the blood of cows suffering from postpartum hypocalcemia. It was found that in the blood of cows with clinical signs of postpartum hypocalcemia ,25- OHD3 content was higher, but the content of PTH and CT - lower in coparisant with healthy cows within 1-2 days after calving. However, in the blood of cows suffering from postpartum hypocalcemia was decreasing concentration of total, protein-linked and ultrafiltrates calcium, inorganic phosphorus, glucose, total protein and was increasing concentration of NEFA, magnesium and activity of alkaline phosphatase.

scholarly journals Biochemical processes in cows and the biological value of milk under influence of cobalt citrate

2017 ◽  
Vol 19 (74) ◽  
Author(s):  
M. Khomyn ◽  
I. Kovalchuk ◽  
S. Kropyvka ◽  
M. Tsap
Keyword(s):  
Milk Production ◽  
Total Protein ◽  
Dry Matter ◽  
Inorganic Phosphorus ◽  
Animal Blood ◽  
Biological Value ◽  
Biochemical Processes ◽  
Mineral Supplements ◽  
Daily Milk Production ◽  
Daily Milk

The aim of research was to study the effect of different amounts of cobalt citrate produced by nanotechnology on biochemical processes in cows, their productivity and biological value of milk in the first two months of lactation. To achieve this goal the subsequent problems have been solved. We investigated the effect of different amounts of cobalt citrate on biochemical parameters of blood and milk of cows and controlled their average daily yield. The experiment has been conducted in 3 groups of cows (5 cows each) of Ukrainian black and white dairy cattle, analogue by body weight (590–620 kg), performance (6,5–6,8 thousand kg of milk per past lactation) and lactation (3–4 lactation). Unlike the control cows, animals experimental (ІІ) group for two months were fed by mineral supplements in the form of cobalt citrate in an amount of 19 mg Co/kg of dry matter of diet and research (ІІІ) – cobalt citrate in an amount of 34 mg Co/kg of dry matter of diet. Duration of cobalt citrate feeding of cows of research groups was 2 months. In all groups of cows the samples of venous blood were taken in preparation for feeding additives period and during 60 day of additives feeding period in order to determine the concentration of total protein, ceruloplasmin, sialic acids, hexoses bound to proteins and activity of amino transferases. The milk production has been controlled in days of sampling with the definition of daily milk yield per cow and taking an average sample for the determination of milk fat, protein, lactose, inorganic phosphorus and calcium. It has been established that the inclusion in the diet of cows of experimental (ІІ) group of mineral supplements contributed to an increase in animal blood total protein content by 11.9% (P < 0.05), inorganic phosphorus by 15.3% (P < 0.05) and hexoses bound protein by 8.8% (P < 0.05). Under these conditions milk content of inorganic phosphorus increased by 5.6% (P < 0.05) and average daily milk production increased by 4.5%. The use of mineral additives in third experimental group of animals within two months contributed to an increase in blood ceruloplasmin content by 5.1% (P < 0.05) and hexoses bound protein by 11.5% (P < 0.01). Mineral supplements promote 5.4% raise of average daily milk production and increase it to the lactose content by 0.10% (in absolute).

scholarly journals Variability of blood serum biochemical parameters in Karakachan sheep

2015 ◽  
Vol 31 (1) ◽  
pp. 55-62 ◽  
Author(s):  
O. Stevanovic ◽  
M. Stojiljkovic ◽  
D. Nedic ◽  
D. Radoja ◽  
V. Nikolic ◽  
...  
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Age Groups ◽  
Balkan Peninsula ◽  
Inorganic Phosphorus ◽  
Sheep Breed ◽  
Mean Values ◽  
Indigenous Sheep ◽  
Significant Difference ◽  
Glutamyl Transferase

Karakachan sheep represents an endangered, indigenous sheep breed from Balkan Peninsula. There is relatively little information about the characteristics of this sheep breed in the veterinary scientific literature. The aim of this research was an examination of certain metabolic profile parameters of the Karakachan sheep blood serum, and variability of their concentrations in comparison to age and some other indigenous sheep breeds from Balkans. Examination was conducted on 14 clinically healthy sheep divided in two age groups. Blood samples were collected by puncture of v. jugularis and blood serum was separated after spontaneous coagulation. The concentrations of total protein, albumin, calcium, inorganic phosphorus, aspartate amino transferase (AST) and ?- glutamyl transferase (GGT) were determined. In relation to age of Karakachan sheep, statistically significant difference between the calculated mean values of examined parameters was not observed. A statistically significant difference was found between the mean concentrations of the studied parameters in Karakachan sheep and other breeds in total protein (Tsigai, Dubrovnik and Dalmatian sheep), albumin (Dalmatian), calcium and inorganic phosphorus (Tsigai, Dubrovnik) and AST's (Dalmatian, Karakachan sheep from Bulgaria).

scholarly journals Serum proteinogram and biochemistry of Holstein cows in the peripartum period

2019 ◽  
Vol 39 (5) ◽  
pp. 342-347
Author(s):  
Thaís G. Rocha ◽  
Camila Bortoletto ◽  
Daniela G. Silva ◽  
Kalina M.M.G. Simplício ◽  
Luiz F. Zafalon ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Total Protein ◽  
Repeated Measures ◽  
Transition Period ◽  
Serum Levels ◽  
Inorganic Phosphorus ◽  
Holstein Cows ◽  
Total Calcium ◽  
Peripartum Period

ABSTRACT: In face of the few reports found in national literature analyzing the potential influence of parturition number in serum proteinogram and biochemical profile in the peripartum period of high yielding dairy cows, the aim of the present study was to comparatively evaluate the dynamics of these serum constituents’ concentrations in blood samples obtained from primiparous and multiparous Holstein cows, 60 and 30 days prepartum and in the day of parturition. Data were analyzed by repeated measures variance analysis (ANOVA) and differences between groups and moments were analyzed by Tukey’s test. Results were considered significant when P<0.05. Parity influenced levels of total protein, albumin, globulins, magnesium, cholesterol, which were higher in multiparous cows, as well as concentrations of ceruloplasmin, total calcium, chloride and alkaline phosphatase activity, which were higher in primiparous cows. Parturition influenced serum concentrations of ceruloplasmin (+58%), transferrin (-25%), haptoglobin (+33%), total protein (-17%), globulins (-25%), immunoglobulin A (-43%), immunoglobulin G (-24%), total calcium (-12%), inorganic phosphorus (-10%), chloride (+5%), sodium (+4%), cholesterol (-23%), triglycerides (-38.6%), as well as activities of aspartate aminotransferase (+14%) and alkaline phosphatase (+28%). A decrease in serum levels of total calcium, inorganic phosphorus, cholesterol and triglycerides was more pronounced in multiparous than in primiparous cows. These results demonstrate that the interpretation of proteinogram and serum constituents should take into consideration lactation number and the moment of parturition as relevant factors in high yielding dairy cows in the transition period.

scholarly journals Protein-synthesizing function of the liver of cows at experimental fasciolosis

2021 ◽  
Vol 4 (1) ◽  
pp. 12-15
Author(s):  
D. V. Frejuk ◽  
V. V. Stybel
Keyword(s):  
Functional State ◽  
Causative Agent ◽  
Total Protein ◽  
The Body ◽  
Control Group ◽  
Blood Proteins ◽  
Functional Connection ◽  
Animal Blood ◽  
The World ◽  
Experimental Group

Cattle fasciolosis is quite widespread in the world, including in Ukraine. Animal blood proteins, being in close functional connection with proteins of various tissues, reflect the changes that occur in the tissues and organs of the body during disorders of their metabolic processes caused by pathological factors. The aim of the study was to investigate the level of total protein and its fractions in the blood of cows in experimental fasciolosis. 12 cows of 4–5 years of age, black-spotted breed, were selected for experiment, out of which 2 groups were formed, six animals in each. Animals in the control group were clinically healthy. Animals of the experimental group were experimentally infected with adolescents. During the research, the rules of compulsory experiments were followed - selection and keeping of analogue animals in groups. The cows' diet was balanced in terms of nutrients and minerals. In the study of protein-sensitizing function of the liver of cows, it was found that during fasciolosis invasion, the content of total protein decreased by 13.8 %. These changes were due to a decrease in albumin and an increase in globulins. Thus, in fasciological invasion of cows, the level of albumin in the serum decreased by 24.4 %, and the level of globulins increased by 7.9 %. The value of the albumin / globulin ratio in the blood of infested cows with the causative agent of fasciolosis is quite indicative. It was found that the albumin-globulin ratio in the blood of cows of the experimental group was probably lower compared to the control group of animals. This value of the coefficient, of course, indicates the suppression of protein-synthesizing function of the liver in these periods of research. In the future it is planned to investigate the functional state of the liver of cows with experimental fasciolosis.

scholarly journals Effect of feeding of chelated zinc form on security, productivity and slaughter parameters of broilers

2021 ◽  
pp. 110-118
Author(s):  
Tetiana Fotina ◽  
Hanna Fotina ◽  
Svitlana Nazarenko ◽  
Roman Tymoshenko ◽  
Oleksii Fotin
Keyword(s):  
Broiler Chickens ◽  
Average Daily Gain ◽  
Experimental Studies ◽  
Live Weight ◽  
Feed Consumption ◽  
Control Group ◽  
Significant Difference ◽  
Veterinary Faculty ◽  
Experimental Group ◽  
Effect Of Feeding

The results of the effect of feeding the chelated form of Zinc on the safety, productivity and slaughter parameters of broiler chickens are presented. The aim of this study was to determine the safety, productivity and slaughter parameters of broilers on the background of feeding chelated form of Zinc. Materials and methods. Experimental studies were conducted during 2020 on broiler chickens cross Cobb-500 in the vivarium of the Veterinary Faculty of Sumy National Agrarian University (Ukraine), divided into two groups on the principle of analogues (control and experimental) of 50 broilers each. The source of zinc for chickens in the control group was their sulfates. Chelated forms of Zinc were used for broiler chickens of the experimental group. All groups of broiler chickens were administered the compound with compound feed in a dose that corresponded to the daily requirement of the bird in this trace element. The duration of the experiment is 42 days. The growth and development of broilers were evaluated on the basis of determining the relevant zootechnical indicators. Live weight of birds was determined by individual weighing on scales of the VNC type with an accuracy of ±1 g at the age of 7, 14, 21, 28, 35 and 42 days. Both scientific and economic experiments also determined the preservation of livestock - daily by the number of culled and dead birds. Feed consumption was recorded daily, for each week of cultivation and for the entire period of the experiment. At the end of the experiment, the cost of feed per 1 kg of live gained weight was calculated. Results. According to the results of the conducted researches, it was established that feeding of the chelated form of Zinc probably increased both the average daily gain and the total live weight of broiler chickens at different age periods of rearing. The live weight of chickens at the beginning of the experiment was almost the same, but at 7 days of age it tended to increase in chickens of the experimental group, although a significant difference between groups in this indicator was not found. It was found that the weight of uneviscerated, semi-eviscerated and eviscerated chicken in poultry of the experimental group was greater than similar indicators in the young animals of the control group by 9.3, 9.2, and 9.8 %, and this difference was significant (p<0.01). The yield of semi-eviscerated carcasses was higher in ones of the experimental group (84.19 %), and the difference was significant (p<0.05). Conclusions. It was found that the live weight of chickens of the experimental group (consumption of feed with a chelated form of Zinc) prevailed at 7 days of age by 1.1 g; on the 14th day – on 22.1 g (p<0.01); at 21 days – 35.4 g (p<0.01); at 28 days – 94.5 g (p<0.05); at 35 days – 133.6 g (p<0.01); at 42 days – at 218.9 g (p<0.05). Preservation of young animals in the experimental group, which used chelated compounds of Zinc, was higher and amounted to 96 %, compared with the control – 92 %. By age, feed costs per unit weight gain were lower in the experiment, respectively: 0.05; 0.08; 0.12; 0.06; 0.07 and 0.05 kg, compared to the control group. Growing broiler chickens on compound feed with a chelated form of Zinc by 9.8 % (p<0.01) increases the weight of the eviscerated carcass.

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