scholarly journals Bacterial and mycotic factors in the pathogenesis of latent endometritis and salpingitis in cows and a decrease in the sanitary quality of milk

2021 ◽  
Vol 36 ◽  
pp. 06036
Author(s):  
A.V. Filatova ◽  
G.M. Firsov ◽  
S.O. Loshchinin ◽  
V.T. Akhmadov ◽  
N.S. Fayzulina

In 82% of cows, contamination of the genitals with conditionally pathogenic microflora of E. coli was established in 17% of cases, S. aureus - 15%, P. mirabilis - 14%, S. pyogenes - 14%, S. pyogenes, R. vulgaris, C. albicans - 14.0%; S. aureus, E. coli, C. albicans - 14.0%; S. aureus, P. mirabilis, E. coli, C. albicans, A. fumigatus, M. racemosus in 12% of cases of latent endometritis. In salpingitis, microorgans were isolated in the association of S. aureus, P. mirabilis, C. albicans in 28.5% of cases; E. coli, P. mirabilis in 27.7% of cases; E. coli, P. vulgaris, C. albicans, A. fumigatus - 25.2%; S. aureus, E. coli, C. albicans, A. fumigatus - 13.1%, the rest in 5.5% of cases. In patients with latent endometritis and salpingitis, fungi were isolated in 29.6% of cases of A. fumigatus and in 70.4% of cases - C. albicans and C. crusei. Milk obtained from sick cows with latent endometritis and salpingitis showed 2 times higher total bacterial contamination than milk obtained from healthy animals.

2021 ◽  
Vol 4 (3) ◽  
pp. 3624-3628
Author(s):  
Oriana Lisette Betancourt- Gallegos ◽  
Renán Ulises Melgarejo Freire ◽  
Juan Agustín Moncada- Herrera ◽  
Javier Agustín Neumann Vásquez ◽  
Enrique Bombal Catalán

In order to protect the hygienic quality of milk and nutrition of the dairy farm, an additional solution to milking hygiene is to control the contamination of the feeders environment. The effectiveness of an FTCMR (DeLaval) PVC feeding table cover on the microbial count was estimated by comparing the mesophilic aerobic bacteria, total coliform, E. coli and butyric spore counts between concrete feeding places (control) and another with the cover. This was done between October to January in a semi-intensive milk production system in Cunco, IX Region (Chile). Sterile swab were taken in areas of 10 cm2 and all counts were performed according to standards methods. Significant differences were obtained between the feeding places according to surface area (p 0.05), in all counts of indicator microorganisms. However, there were no statistical differences (p 0.05) between counts per month of sampling, except for the E. coli indicator (p = 0.001). In conclusion, the presence of the cover would positively influence the reduction of indicator microorganisms at the feeding level between October to January, which would protect the quality of the forage, and the hygiene and duration time of the milk.


1954 ◽  
Vol 17 (7) ◽  
pp. 204-218 ◽  
Author(s):  
C. H. Allen ◽  
F. W. Fabian

Since the discovery of the role that bacteria play in disease, a search has been made for a suitable organism that would be indicative of dangerous bacterial contamination in food. Early work with pathogenic bacteria indicated that they would not be suitoble to serve this purpose since they were hard to isolate, were too fastidious, and died off quickly under conditions favorable for keeping food. For these and other reasons the search turned to other bacteria associated with pathogenic bacteria but which were hardier and easier to work with and identify. Fullfilling these requirements were two organisms, E. coli and Strept. faecalis, normal inhabitants of the intestinal tract and associated with many pathogenic bacteria especiallv the enteric bacteria which ore responsible for many food outbreaks. Today the presence of moderate numbers of these organisms is not considered a sure sign of dangerous contamination but rather of possible contamination. Their presence indicates that a search for the cause of the contamination should be made.


Author(s):  
Yunita Rusidah ◽  
Lailatul Farikhah

This study is intended to find the quality of bottled drinking water (AMDK) and drinking water (AMIU) sold around University of Muhammadiyah Kudus, in terms of organoleptic and microbiological parameters in drinking water according to SNI 01-3554-2006 and PMK No. 492 of  2010. The test is carried out using 10 sample of drinking water. They are 7 sample of AMDK (Ades, Airmu, Aqua, Cleo, Crystalin, Le mineral and Vit) and 3 sample of AMIU (Pasuruhan, Prambatan and Purwosari depots). The research consists of organoleptic and  mikrobiologi (TPC, presence of E. coli and total coliform). The results of research shows that the organoleptik of all samples is in normal condition. Whereas, microbiologically, the TPC results before dilution were only Cleo and Ades which were suitable for consumption because they were absent from bacterial contamination. While the TPC results after dilution, testing the difference between E. Coli and Total coliform resulted in 7 samples of bottled drinking water which were safe and feasible as drinking water according to SNI 01-3553-2006 and 3 samples AMIU does not fulfil PMK No. 492/2010, as drinking water requirements.


1988 ◽  
Vol 55 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Charles H. McKinnon ◽  
A. John Bramley ◽  
Steven V. Morant

SummaryThe development of devices to sample milk at the clawpiece and at the end of the pipeline during milking are described. The results of tests to compare the bacteriological and compositional quality of milk collected from the sampling devices or from the recorder jar or milk cans were similar, demonstrating that the samples provided accurate and representative values. The use of the in-line sampling technique, whereby samples of the milk are taken at three points during its passage through the milking plant, to assess accurately the sources of bacterial contamination of bulk tank milk on farms is discussed.


MEDIAGRO ◽  
2019 ◽  
Vol 14 (2) ◽  
Author(s):  
Any Widiyastuti ◽  
Dian Wahyu Harjanti ◽  
Bhakti Etza Setiani

This research was conducted to evaluate the bacterial contamination in fresh milk from farmers, milk collectors, and dairy cooperative (KUD) in Getasan Subdistrict, Semarang Regency. The evaluation of bacterial contamination has been observed by the number of bacteria, milk distribution time, reductation test, alcohol test and pH test of fresh milk samples which had been taken from 30 farmers, 30 milk collectors and 4 dairy cooperatives in Getasan Subdistrict. The result showed that in the farmers, there is 1 positive alcohol test from 30 samples, pH 6, reductation time of 7 hours, and has total bacteria 3.01 × 105 cfu / ml milk. The alcohol test of the milk from collectors also show 1 positive result from 30 samples with pH 6, reduction time of 6 hours and has total bacteria 6.17 × 105 cfu / ml milk. While in alcohol test of dairy cooperative shows 2 positive result, from 4 samples with pH 6, reductation time of 5 hours and has total bacteria 1.38 × 106 cfu / ml milk. The duration of milk distribution from farmer to KUD were 111 minutes. Thus, it is concluded that microbiological quality of milk in farmers and milk collectors level is good because it is below the maximum limit of total bacteria according to Indonesian Standart, but the amount of bacteria milk contamination in dairy cooperative is high due to the duration of distribution (111 minutes) without cooling.Keywords: bacterial count, milk quality, milk safety, distribution time


Author(s):  
V.N. Podrez ◽  
M.A. Lytina

Introduction into the daily milking procedure of the hygienic product “ItalmasVP - I 2500”, based on an iodine-polymer complex, which has a disinfecting and regenerating-healing effect for the treatment of the udder teats after milking,allows to reduce the level of bacterial contamination of the udder and improve the quality of milk by reducing bacterial contamination of milk and decreasing the number of somatic cells. “ItalmasVP - I 2500” has a high prophylactic efficacy for the incidence of mastitis in cows and ensures a longer hygienic protection of the cow’s udder teats.


1999 ◽  
Vol 66 (4) ◽  
pp. 627-631 ◽  
Author(s):  
LUBOV Yu. BROVKO ◽  
VALERY G. FROUNDJIAN ◽  
VERONIKA S. BABUNOVA ◽  
NATALYA N. UGAROVA

Enumeration of bacteria in raw milk is of public health and economic importance. Among the proposed rapid methods for assessment of bacterial contamination in raw milk, ATP bioluminescence has proved to be one of the most promising (Griffiths, 1991). Several companies produce ATP bioluminescence reagent kits and equipment for analysing raw milk samples for total bacterial count (Sutherland et al. 1994; Reybroeck & Schram, 1995). The principle of ATP bioluminescent bacterial assay is based on the following assumptions (Olsen, 1991). All living organisms contain ATP, ATP is neither associated with dead cells nor absorbed on to surfaces, colloids and so on, and there is a fairly constant ratio of ATP to biomass/number of cells for all microbial taxa independent of metabolic activity or environmental conditions. Of these assumptions, only the first seems to be indisputable. It is not the number of bacterial cells, but rather the colony forming unit (cfu) that is the denomination used when assessing the microbial quality of milk. For Gram-negative rods of the genus Enterobacteriaceae, a cfu is usually derived from a single cell. However, Gram-positive cocci (staphylococci and streptococci) grow in bunches and chains respectively (Gregg, 1991), and estimation of cell numbers may not give good agreement with the colony counts.Several approaches have been investigated to increase the sensitivity of the bioluminescent method (Pahuski et al. 1991; Sutherland et al. 1994; Reybroeck & Schram, 1995; Froundjian et al. 1999). Although the detection limit achieved by these modifications (104 cfu/ml) may be sufficient for practical use (Bautista et al. 1992; Reybroeck & Schram 1995), the accuracy of the analysis was not significantly improved. The reported values for accuracy of the estimate for cfu/ml in raw milk (Syx) by the bioluminescent method were in the range 0·27–0·87 log units (Bautista et al. 1992; Reybroeck & Schram, 1995). The purpose of the present study was to determine the reasons for the lack of accuracy of the bacterial ATP assay in raw milk.


Sign in / Sign up

Export Citation Format

Share Document