PARTICULARITIES OF FOLLICULOGENESIS DURING THE OESTROUS CYCLE IN COWS WITH THE REPEAT BREEDING SYNDROME

Particularities of folliculogenesis during the oestrous cycle in cows with the repeat breeding syndrome were detected. It was found that the repeat breeders that previously had suffered from endometritis or retention of the placenta, as well as animals with post-oestrual metrorrhagia show considerable shifts in the dynamics of follicular growth both with two and three waves of follicular development. In repeat breeders with no genital patho-morphological abnormalities there were no found considerable changes in the dynamics of follicular development compared to healthy animals. The two-wave oestrous cycles were registered in 70.6% of repeat breeders in the absence of clinical signs of genital pathology; in 50% of cows with the repeat breeding syndrome that previously had had endometritis or retention of placenta; in 66.7% of cows with post-oestrual metrorrhagia and in 66.7% of healthy animals. In cows with two waves of follicle growth, the duration of the oestrous cycle was shorter (the difference between healthy animals and cows with post-oestrual metrorrhagia made 3.4 days, P<0.025). The duration of the 1st wave of follicular growth was shorter in animals with the three-wave follicular growth. The maximum duration of the 1st wave of follicular growth was registered in the repeat breeders that previously had endometritis or retention of placenta, as well as in the animals with post-oestrual metrorrhagia (7.6 and 8.3 days against 6.4 days in healthy cows, respectively, P<0.025). The duration of the 1st wave of follicular growth in cows with two waves of follicular development did not differ significantly. In healthy cows with two waves of follicular growth, in comparison with animals with the repeat breeding syndrome, the second growth wave was longer and averaged 10.5 days. It was the shortest in cows with post-oestrual metrorrhagia (7.3 days, P<0.05). The duration of this wave was significantly shorter in cows with endometritis and retention of placenta (8.6 days, P<0.05). In healthy cows with three growth waves of follicles, the duration of the 2nd growth wave was 8.6 days. The most significant changes in follicle growth were found in repeat breeders with three growth waves that had suffered from endometritis or retention of placenta, and in animals with the repeat breeding syndrome,and post estrual metrorrhagia. In cows with post-oestrual metrorrhagia the duration of the 2nd growth wave in comparison with healthy animals was shorter by 2.4 days (P>0.05), and the third wave, on the contrary, was longer by 1.2 days (P<0.1).

COMPLEX TREATMENT OF COWS WITH RUSTERHOLZ ULCERS USING ADIPOSE-DERIVED MESENCHYMAL STEM CELLS

The use of adipose-derived mesenchymal stem cells (MSC AT) in treatment of animals with Rustergolz ulcer is determined by the anti-inflammatory properties of these cells, their ability to increase angiogenesis and stimulate internal progenitor cells to regenerate tissue functions. These properties allow to reduce the healing time of ulcerative lesions of the hooves in cows, therefore it is advisable to use a treatment regimen with the use of mesenchymal stem cells.

EFFICIENCY OF DIAGNOSIS AND COMPLEX TREATMENT OF PYOMETRA IN DOG FEMALES

Pyometra occurs in 25% of cases in dogs of different breeds, body weight, and a number of parturitions. We have found that the use of homeopathic preparations in combination with humilide in a complex treatment of pyometra allows to delay or minimize the implementation of surgical methods.

USE OF SCANNING ELECTRON MICROSCOPY FOR ASSESSMENT OF PATHOGENIC CHARACTERISTICS OF CHICKEN INFECTIOUS ANEMIA VIRUS

With the use of a scanning electron microscopy, the morphological evaluation of pathogenic properties of the chicken infectious anemia virus was carried out. It is demonstrated that this virus induces a number of structural dis-turbances in chicken body.

MORPHOLOGICAL CHANGES IN KIDNEYS OF CHICKENS IN THE NEPHROSIS-NEPHRITIC FORM OF INFECTIOUS BRONCHITIS

The article presents data on the study of pathomorphological changes in the kidney of chickens with nephrosis-nephritic form of infectious bronchitis.The formation of protein cylinders of various structures in the renal parenchyma is found. There are also marked serous glomerulitis, vacuolar and granular degeneration, necrobiosis and necrosis of certain portions of the kidney tubules, an increase in the indicators of the plasmacytic reaction.

TOXICOLOGICAL PROPERTIES AND THERAPEUTIC EFFICACY OF THE VETERINARY DRUG “MASTILACT LC” AT MASTITES IN COWS

Toxicological properties were studied and production tests were conducted of a veterinary drug “Mastilact LC”. The findings show that by the indicators of acute intragastric toxicity, the drug is referred to the IV class of danger. The therapeutic efficacy of the drug was 87.5%, the course of treatment averaged from 3.25±0.16 to 4.88±0.30 days, depending on the form of mastitis. The drug possesses no negative impact on animal body.

EFFICACY OF A VETERINARY PREPARATION “VITOL-140” IN COMPLEX TREATMENT OF DOGS AND CATS WITH DERMATITES

The use of a veterinary preparation “Vitol-140” in complex therapy of dogs and cats suffering from dermatites promotes normalization of hematological parameters, improves the time of recovery and increases the efficacy of therapy.

СOMPLEX TREATMENT OF CATTLE WITH PURULENT CONJUNCTIVO-KERATITES

The application of a nonwoven nanomaterial with tylosin and propolis 0.5×1 cm in size into the conjunctival sac once every three days in combination with the intramuscular administration of catosal at a dose of 10 ml within 5 successive days of a complex therapy improves recovery time in cattle with purulent conjunctival keratites and increases the cost-effectiveness of treatment.

PATHOMORPHOLOGY, DIAGNOSTICS AND SPECIFIC PREVENTION OF VIRAL RESPIRATORY AND ABOMASOENTERITIC INFECTIONS IN CALVES

Viral diseases in calves are common and often occur in associations. Pathomorphological changes in organs and tissues at viral infections allow making a preliminary nosological diagnosis and develop therapeutic and preventive measures to eliminate diseases. A single immunization of cows with a live-virus vaccine by the “carpet method” prevents the infection of calves in utero, and increases the safety rate of newborn calves.

THE INFLUENCE OF A COMPLEX APIPREPARATION “APHRODITE” ON BLOOD PARAMETERS OF PREGNANT COWS

The findings show that in deep pregnant cows of the Red Steppe breed changes of biochemical values of blood were mild; however, the acid-base imbalance, disturbance in mineral and protein metabolism, and a decrease in the carotene status were detected. The use of the apipreparation “Aphrodite” for prophylactic purposes contributed to the increase in the calcium level by 1.11 times, immunoglobulin M by 1.28–1.48 times, and lysozyme activity by 14.46%. Therefore, this preparation showed a pronounced immune stimulating effect.