scholarly journals Genetic variation of CAST gene in Local Karnobat and Karnobat merino sheep breeds

2022 ◽  
Vol 42 ◽  
pp. 01029
Author(s):  
Ivona Dimitrova ◽  
Milena Bozhilova-Sakova ◽  
Margarit Iliev ◽  
Atanaska Teneva
Keyword(s):  
Genetic Material ◽  
Specific Enzyme ◽  
Blood Samples ◽  
Merino Sheep ◽  
Sheep Breeds ◽  
Heterozygous Individual ◽  
Pcr Rflp ◽  
Allele Variation ◽  
Southeastern Region ◽  
Cast Gene

Karnobat sheep plays an important role in the development of sheep breeding in Southeastern region of Bulgaria. They are valuable source of genetic material. The aim of present experiment was to determine the allele variation of CAST gene in Local Karnobat and Karnobat Merino sheep breeds. A total of 60 blood samples were collected – 30 per breed. DNA was extracted and genotypes of all animals were identified by means of PCR-RFLP technique. The restriction reactions were accomplished by specific enzyme MspI. As expected both breeds were characterized with low level of genetic diversity due to the fact that mostly maintaining selection has been implemented. In Local Karnobat sheep breed was identified only one heterozygous individual from all 30. In Karnobat merino were identified allele M with frequency 0,97 and allele N with frequency 0,03. Genotypes MM and MN were revealed with frequencies 0,93 and 0,07, respectively. According to the statistical analysis both breeds were in HWE equilibrium.

scholarly journals Determination of the Polymorphism of the Booroola Fecundity B, Callipyge and Calpastatin Genes in Assaf Sheep Breed by PCR-RFLP Method

2020 ◽  
Author(s):  
Omar M. Darissa ◽  
Raeda Irekat
Keyword(s):  
Low Frequency ◽  
High Growth ◽  
Allelic Frequency ◽  
Sheep Breed ◽  
Salting Out ◽  
High Fecundity ◽  
Blood Samples ◽  
Sheep Breeds ◽  
Meat Content ◽  
Pcr Rflp

Background: The development of local sheep breeds that have high fecundity levels, high meat content and high growth rates would help satisfy the Palestinian demand of meat and lower its price. It has been reported that mutant alleles of the genes Booroola fecundity (FecB), Callipyge (CLPG) and calpastatin (CAST) are associated with high litter size, larger muscular rumps and higher tenderness of meat after sheep slaughter, respectively. PCR-RFLPs methods have been established to facilitate the survey of sheep breeds for such mutants.Methods: In this study, 117 blood samples of the most common sheep breed in Palestine, Assaf, were collected from Bethlehem and Jenin districts. Genomic DNA was extracted and purified from the blood samples using the salting out method. The genotypic ratios and allelic frequencies of the FecB, CLPG and CAST genes were determined by PCR-RFLP method. Result: The results show that the mutant allele B of the FecB gene has a very low frequency among the screened sheep with 0.01 in Bethlehem and 0.07 in Jenin. Also, 94.4% of the tested sheep have the wildtype genotype AA for the CLPG gene with a frequency of 0.95 for allel A. Moreover, the desired heterzygous genotype MN for the CAST gene is 38.4% with an allelic frequency of 0.81 for the mutant allel M. These results would assist establishing sheep breeding programs with the appropriate parental genotypes to improve the livestock sector in the country. 

scholarly journals Polymorphism of CAST gene in five sheep breeds in Bulgaria

2021 ◽  
Vol 262 ◽  
pp. 02026
Author(s):  
Ivona Dimitrova ◽  
Milena Bozhilova-Sakova ◽  
Atanaska Teneva ◽  
Tanya Ivanova ◽  
Ina Stoycheva
Keyword(s):  
Genetic Diversity ◽  
Genotypic Variation ◽  
Ovis Aries ◽  
Heterozygous Genotype ◽  
Breeding Programs ◽  
Candidate Marker ◽  
Sheep Breeds ◽  
Homozygous Genotype ◽  
Pcr Rflp ◽  
Cast Gene

CAST gene is a candidate marker that influences the intensity of growth and meat quality. The aim of this study was to investigate the genetic variability of CAST gene in five Bulgarian sheep breeds – two merino, two local and one for milk. A total of 150 ewes, belonging to these breeds were investigated for polymorphisms of CAST gene by using PCR-RFLP method. A 622 bp fragment of Ovis aries CAST gene was amplified using PCR. After restriction with endonuclease MspI two alleles were observed in Ascanian, Caucasian, Breznik and Pleven Blackhead breeds. One allele (M) and one genotype (MM) were detected in Cooper-Red Shumen breed. The highest frequency of the allele N was established in the Ascanian merino breed (0.27) followed by Caucasian merino (0.13), Breznik (0.12) and Pleven Blackhead (0.07). Two genotypes - MM and MN, were observed in Caucasian, Breznik and Pleven Blackhead breeds. The three possible genotypes were found only in Ascanian merino ewes - MM, MN and NN. The lowest frequency of the homozygous genotype MM (0.50) and the highest frequency of the heterozygous genotype MN was established in the Ascanian merino breed (0.47). The obtained results confirm that the PCR-RFLP method can be used to identify different genotypic variation of CAST gene in Bulgarian sheep breeds. The established genetic diversity in the calpastatin gene indicates that, after further associative studies, this gene may be included in the breeding programs of certain sheep breeds.

β-Lactoglobulin Gene Types in Karacabey Merino Sheep Breeds Using PCR-RFLP

2007 ◽  
Vol 32 (2) ◽  
pp. 145-148 ◽  
Author(s):  
Cengiz Elmaci ◽  
Yasemin Oner ◽  
M. S. Balcioglu
Keyword(s):  
Merino Sheep ◽  
Sheep Breeds ◽  
Pcr Rflp ◽  
Β Lactoglobulin

scholarly journals Survey of ß-Lactoglobulin and α<sub>S1</sub>-Casein polimorpbisms in Hungarian dairy sheep breeds and crosses on DNA level (short communication)

1999 ◽  
Vol 42 (4) ◽  
pp. 387-392 ◽  
Author(s):  
I. Anton ◽  
A. Zsolnai ◽  
L. Fesús ◽  
S. Kukovics ◽  
A. Molnár
Keyword(s):  
Milk Yield ◽  
Milk Protein ◽  
Short Communication ◽  
Dairy Sheep ◽  
Blood Samples ◽  
Cheese Making ◽  
Sheep Breeds ◽  
Pcr Rflp ◽  
Beta Lactoglobulin

Abstract. Beta-lactoglobulin (LGB) and the Welsh αS1-casein (αS1-casein D) types have been determined using PCR-RFLP tests in DNA isolated from blood samples collected from four dairy sheep breeds (Awassi, British Milk Sheep, Tsigaia, Lacaune), from Hungarian Merinos and from various crossbreeds (AwassixMerino) F1, (MerinoxLanghe) F1, (MerinoxPleven Blackhead) F1, (MerinoxPleven Blackhead ) F1x Black East Friesian). The following LGBA frequency values have been obtained: Awassi 0.3478; British Milk Sheep 0.6857; Tsigaia 0.5650; Lacaune 0.4730; Hungarian Merino 0.6767; (Awassix Merino) F1 0.4694; (MerinoxLanghe) F1 0.7777; (MerinoxPleven Blackhead) F1 0.5945; (MerinoxPleven Blackhead) F1 x Black East Friesian 0.6026. The Welsh αS1-casein variant was found in the Hungarian Merino breed only (4 homozygous and 16 heterozygous ewes). Plans for studies on the possible effects of these milk protein types, on milk yield and composition as well as on cheese making properties ofmilk and cheese yields are outlined.

PSXII-2 Identification of candidate SNPs associated with high prolificacy in Romanov sheep

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 254-255
Author(s):  
Alexey V Shakhin ◽  
Arsen V Dotsev ◽  
Tatiana E Deniskova ◽  
Gottfried Brem ◽  
Natalia A Zinovieva
Keyword(s):  
Higher Education ◽  
Genetic Basis ◽  
Reference Genome ◽  
Unique Property ◽  
Candidate Snps ◽  
Merino Sheep ◽  
Sheep Breeds ◽  
Important Trait ◽  
Very High ◽  
Selection Of

Abstract Prolificacy is a very important trait in sheep. Romanov sheep, the well-known Russian sheep breed, are characterized by very high prolificacy; however, the genetic basis of this unique property of Romanov sheep is still unknown. It was reported that Ovine BMPR1B gene, located on the OAR6, is associated with prolificacy in several sheep breeds. The aim of our study was to identify candidate SNPs within BMPR1B gene, related to prolificacy. To achieve this goal, using NGS technology, we sequenced ovine BMPR1B gene in Romanov sheep (n = 6), which are characterized by high prolificacy (about 270 lambs per 100 ewes). The sequences of BMPR1B gene of Noire du Velay, Tan, Southdown and Australian Horned Merino sheep breeds as well as Asiatic mouflon (n = 1), which are characterized by significantly lower prolificacy (from 110 to 180 lambs per 100 ewes) were derived from publicly available sources and used for comparison. FST analysis performed in PLINK 1.9 program revealed 10 SNPs with values higher than 0.8. The majority of candidate SNPs under putative selection were localized in the region from 29,382,098 to 29,430,387 on OAR6 of Ovine reference genome (Oar_v3.1 (Ensembl release 98). Thus, we can suggest, that this region of the BMPR1B gene can be considered as the putative region, associated with high prolificacy of Romanov sheep. Additional studies will be needed to confirm the effect of identified candidate SNPs on prolificacy traits. The research results will be useful for artificial selection of sheep with higher prolific capacity, including the introduction of desired alleles in sheep populations using genome editing technologies. This work was supported by the Russian Ministry of Science and Higher Education No. 0445-2019-0024 and RFBR No. 20-516-56002.

scholarly journals Analysis of KatG Ser315Thr Mutation in Multidrug Resistant Mycobacterium tuberculosis and SLC11A1 Polymorphism in Multidrug Resistance Tuberculosis in Central Development Region of Nepal Using PCR-RFLP Technique: A Pilot Study

1970 ◽  
Vol 1 (1) ◽  
pp. 14-21 ◽  
Author(s):  
Raunak Shrestha ◽  
Rubin Narayan Joshi ◽  
Kriti Joshi ◽  
Bal Hari Poudel ◽  
Bhupal Govinda Shrestha
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Whole Blood ◽  
Diagnostic Technique ◽  
Molecular Diagnostic ◽  
Multi Drug Resistance ◽  
Base Line ◽  
Blood Samples ◽  
Pcr Rflp ◽  
Development Region ◽  
Whole Blood Samples

Ser315Thr mutations in genes encoding the mycobacteria catalase-peroxidase (KatG) has been associated with the major resistance to isoniazid (INH) in Mycobacterium tuberculosis (MTB). Also G/C polymorphisms in INT4 region of the solute carrier family 11 member 1 gene (SLC11A1) and susceptibility towards tuberculosis (TB) has been demonstrated worldwide. 24 drug resistant MTB culture positive samples and 24 whole?blood samples were collected from different TB patients of Central Development Region of Nepal in 2009. A Polymerase Chain Reaction (PCR) - Restriction Fragment Length Polymorphism (RFLP) assay was carried out in order to investigate Ser315Thr KatG mutation and G/C polymorphism in INT4 region. 4 (16.67%) samples out of 24 MTB culture samples demonstrated the Ser315Thr KatG mutation whereas none of the 24 whole blood samples were found to contain G/C polymorphism in INT4. Though no significant correlation could be found between INT4 polymorphism and TB susceptibility, overall scenario of Nepal cannot be drawn from this data. Molecular diagnostic technique such as PCR-RFLP can be used in a robust scale to carry out base line studies in the TB population of Nepal. Key words: Multi?drug resistance; Tuberculosis; PCR; RFLP Nepal Journal of Biotechnology. Jan. 2011, Vol. 1, No. 1 : 14-21

scholarly journals FEATURES OF DIAGNOSIS OF NECROBACTERIOSIS OF COWS BY PCR-RFLP

2020 ◽  
pp. 26-37
Author(s):  
O.D. Biriukova ◽  
T.M. Suprovych ◽  
M.P. Suprovych ◽  
S.V. Laiter-Moskaliuk ◽  
I.O. Chornyi
Keyword(s):  
Molecular Genetic ◽  
Experimental Sample ◽  
Exon 2 ◽  
Blood Samples ◽  
Restriction Fragments ◽  
Black And White ◽  
Pcr Rflp ◽  
Dairy Breed ◽  
Established Technique ◽  
Gene Exon

Molecular genetic markers can detect polymorphism at the DNA level. This feature determines the possibility of their widespread use in genetics and breeding. Alleles of the BoLA-DRB3 gene (exon 2) can act as such markers if a statically significant association between the disease and the allele is established. The presence of such DNA markers in the genotype of animals makes it possible to judge the likelihood of disease in postnatal ontogenesis immediately after the birth of a heifer, based on which we can conclude about the conditions of further use of the animal in the main herd. According to the results of studying the polymorphism of the BoLA-DRB3 gene in cows of the Ukrainian black and white dairy breed resistant and susceptible to necrobacteriosis, four "informative" alleles were revealed. Two of them *03 and *22 are associated with resistance, and the other two - *16 and *23 with susceptibility to necrobacteriosis. The presence of these alleles in the genotype of the animal is determined by testing performed by PCR-RFLP. The method is time consuming, labor intensive and costly. To simplify it, the following technique is proposed. Restriction fragments of alleles *03, *16, *22 and *23 for endocluases RsaI, XhoII and HaeIII have the following DNA patterns: bbb, jbd, mba and nba. Due to the peculiarity of the restriction fragments, which is that endonuclease XhoII reveals in these alleles only one pattern b with length of 284 bp, the process of determining informative alleles can be simplified. Isolation of DNA from blood samples and amplification of a fragment of the BoLA-DRB3.2 gene with a size of 284 bp is carried out according to the established technique. Next, the restriction of the fragment by endonuclease XhoII and sampling having a pattern b. Selected samples are treated with RsaI endonuclease and only those with patterns b, j, m and n remain. The next step is to restrict the selected samples with HaeIII endonuclease and select heifers with bbb (*03) and nba (*23) genotypes. After the first restriction, blood samples without pattern b are eliminated from the experimental sample; after the second – two alleles with patterns RsaI + XhoII jb (*16) and mb (*22) are unambiguously determined, after the third – genotypes bbb and nba, which correspond to alleles *03 and *23. In total, only 75% of blood samples are typed, which reduces the material consumption, time and cost of work to identify heifers genetically susceptible (resistant) to necrobacteriosis.

scholarly journals Identifikasi Keragaman Gen FTO Pada Bangsa Sapi Potong Indonesia

2019 ◽  
Vol 6 (2) ◽  
pp. 232
Author(s):  
Sutikno Sutikno ◽  
Rudy Priyanto ◽  
Cece Sumantri ◽  
Jakaria Jakaria
Keyword(s):  
Beef Cattle ◽  
Genetic Markers ◽  
Direct Sequencing ◽  
Nucleotide Position ◽  
Fto Gene ◽  
Blood Samples ◽  
Pcr Rflp ◽  
Gene Functions ◽  
Hardy Weinberg Equilibrium ◽  
Cattle Blood

ABSTRAK Gen FTO berfungsi sebagai regulasi homeostasis, deposisi lemak dan pengaturan obesitas. Penelitian ini bertujuan untuk mengidentifikasi polimorfisme SNP g.125550A>T di ekson 3 gen FTO pada bangsa sapi potong Indonesia. Sampel darah diperoleh dari 209 ekor sapi, terdiri atas sapi bali (44), madura (20), Pesisir (20), katingan (20), Peranakan ongole (PO) (22), Pasundan (20), Sumba Ongole (SO) (11), brahman (20), simental (15), dan limousin (18). Polimorfisme gen FTO dianalisis menggunakan metode PCR-RFLP (HpyCH4III) dan direct sequencing. Hasil genotiping SNP g.125550A>T adalah polimorfik (genotipe AA, AT, dan TT) pada sapi madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, dan limousin. Frekuensi alel A dan T masing-masing adalah 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 dan 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31. Nilai Ho dan He masing-masing adalah 0,60-0,14 dan 0,44-0,18 serta dalam keseimbangan Hardy-Weinberg (P>0.05). Sementara pada sapi bali bersifat monomorfik hanya bergenotipe AA. Hasil sekuensing SNP g.125550A>T ditemukan mutasi tranvesi A menjadi T pada posisi nukleotida  g.125550. Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa SNP 125550A>T gen FTO beragam dan berpotensi dijadikan marka genetik untuk kualitas daging pada bangsa sapi potong Indonesia.Kata Kunci: gen FTO, PCR-RFLP, Sapi, SNP g.125550A>TABSTRACTThe FTO gene functions as regulation of homeostasis, fat deposition and regulation of obesity. This study aimed to identify the polymorphism of SNP g.125550A>T in exon 3 of FTO gene in Indonesian beef cattle. Blood samples were collected from 209 cattle, including bali (44), madura (20), pesisir (20), katingan (20), PO (22), pasundan (20), SO (11), brahman (20), simental (15), and limousin (18). Polymorphism of the FTO gene was analyzed using PCR-RFLP (HpyCH4III) and direct sequencing methods. The results of genotyping SNP g.125550A>T was polymorphic (AA, AT and TT genotypes) in madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, and limousin cattle. The frequency of A and T alleles were 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 and 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31 respectively. The values of Ho and He were 0,60-0,14 and 0,44-0,18 respectively and in Hardy-Weinberg equilibrium (P>0,05). While in Bali cattle was monomorphic (AA genotype). Results of sequencing SNP g.125550A>T of the FTO gene found a transverse mutation A to T at the nucleotide position g.125550. As a result of this study, it can be concluded that SNP 125550A>T of the FTO gene was diverse and potentially used as genetic markers for meat quality in Indonesian beef cattle.Keywords: cattle, FTO gene, PCR-RFLP, SNP g.125550A>T.

scholarly journals The status of the Hungarian Merino sheep breed compared to the other merino breeds

2016 ◽  
pp. 151-156
Author(s):  
Mária Szabó ◽  
Szilvia Kusza ◽  
István Csízi ◽  
István Monori
Keyword(s):  
Gene Pool ◽  
Genetic Resource ◽  
Sheep Breed ◽  
The Other ◽  
Drastic Reduction ◽  
Merino Sheep ◽  
Sheep Breeds ◽  
The World ◽  
The Status ◽  
Over Time

Merino and Merino-derived sheep breeds have been widely known and distributed across the world, both as purebred and admixed populations. They represent a diverse genetic resource which over time has been used as the basis for the development of new breeds. In spite of this, their gene-pool potential is still unexplored. The Merino sheep represent the most important sheep resource of the Hungarian husbandry. It has the largest amount of individuals between both of the stock and commercial flocks. But in Europe the Merino stocks went through a drastic reduction in number. Thus these breeds became endangered in several countries as well as in Hungary. In this study we would like to present the recent status of different Merino breeds of the world to ground our further phylogenetic research with the Hungarian Merino breed.

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