Raw and wine processed Schisandra chinensis regulate NREM-sleep and alleviate cardiovascular dysfunction associated with insomnia by modulating HPA Axis

Planta Medica ◽  
2021 ◽  
Author(s):  
Dan Su ◽  
Jian Luo ◽  
Junqi Ge ◽  
Yali Liu ◽  
Chen Jin ◽  
...  
Keyword(s):  
Cardiovascular System ◽  
Hpa Axis ◽  
Nrem Sleep ◽  
Chemical Components ◽  
Schisandra Chinensis ◽  
Cardiovascular Dysfunction ◽  
Melatonin Receptor ◽  
Theoretical Support ◽  
Rat Pituitary ◽  
Electrocardiogram Ecg

Clinical studies have shown that insomnia and anxiety are usually accompanied by cardiovascular dysfunction. In traditional Chinese medicine, Schisandra chinensis (SC) and wine processed Schisandra chinensis (WSC) are mainly used for the treatment of dysphoria, palpitation and insomnia. However, little attention was paid to its mechanism. In this study, we monitored the effect of SC and WSC on the nervous system and cardiovascular system of free-moving rats in the real-time. Our results show that SC and WSC can alleviate cardiovascular dysfunction while promoting sleep, and we further explored their potential mechanisms. HPLC-QTOF-MS was used for the quality control of chemical components in SC and WSC. Data sciences international (DSI) physiological telemetry system was applied to collect the electroencephalogram (EEG), electrocardiogram (ECG) and other parameters of free-moving rats to understand the effects of long-term intake of SC and WSC on rats. The content of Cortisol (CORT), neurotransmitters and amino acids in rat pituitary and hypothalamus were analyzed by UPLC-MS to determine the activity of HPA axis. The expression of melatonin receptor MT1 was analyzed by immunofluorescence technique. Our results suggested that SC and WSC may play the role of promoting sleep by increasing the expression level of melatonin receptor MT1 in hypothalamus, and modulate the activity of HPA axis by regulating the levels of the related neurotransmitters and amino acid, so as to improve the abnormal cardiovascular system of rats. This study may provide theoretical support for explicating the advantages of SC and other phytomedicines in the treatment of insomnia.

Chemical Components Composition on Different Parts of Fruit in Schisandra chinensis Baillon

2016 ◽  
Vol 45 (6) ◽  
pp. 851-858 ◽  
Author(s):  
Ka Soon Lee ◽  
Bo Hee Lee ◽  
Bong Jae Seong ◽  
Sun Ick Kim ◽  
Seung Ho Han ◽  
...  
Keyword(s):  
Chemical Components ◽  
Schisandra Chinensis ◽  
Different Parts

Chemical components of male and female flowers of Schisandra chinensis

2020 ◽  
Vol 92 ◽  
pp. 104121
Author(s):  
Kandhasamy Sowndhararajan ◽  
Ju-Ho Kim ◽  
Ji Eun Song ◽  
Minju Kim ◽  
Songmun Kim
Keyword(s):  
Chemical Components ◽  
Schisandra Chinensis ◽  
Male And Female ◽  
Female Flowers

Differences in habituation to psychosocial stress between the HPA-axis, the cardiovascular system and peripheral catecholamines

2000 ◽  
Vol 25 ◽  
pp. S53 ◽  
Author(s):  
Nicole C. Schommer ◽  
Matthias Kuhr ◽  
Dirk H. Hellhammer ◽  
Rainer Luedke ◽  
Ulrike Rothe ◽  
...  
Keyword(s):  
Cardiovascular System ◽  
Hpa Axis ◽  
Psychosocial Stress

scholarly journals The mt1 Melatonin Receptor and RORβ Receptor Are Co-localized in Specific TSH-immunoreactive Cells in the Pars Tuberalis of the Rat Pituitary

2002 ◽  
Vol 50 (12) ◽  
pp. 1647-1657 ◽  
Author(s):  
Paul Klosen ◽  
Christele Bienvenu ◽  
Olivier Demarteau ◽  
Hugues Dardente ◽  
Hilda Guerrero ◽  
...  
Keyword(s):  
Cell Types ◽  
Orphan Receptor ◽  
Seasonal Effects ◽  
Pars Tuberalis ◽  
Melatonin Receptor ◽  
Functional Studies ◽  
Rat Pituitary ◽  
Mt1 Melatonin Receptor

The pars tuberalis (PT) of the pituitary represents an important target site for the time-pacing pineal hormone melatonin because it expresses a large number of mt1 receptors. Functional studies suggest that the PT mediates the seasonal effects of melatonin on prolactin (PRL) secretion. The aim of this study was the characterization of the pheno-type of melatonin-responsive cells. Furthermore, we determined whether RORβ, a retinoid orphan receptor present in the PT, was co-expressed in the same cells. We combined nonradioactive in situ hybridization (ISH) with hapten-labeled riboprobes for detection of the receptors and immunocytochemistry (ICC) for detection of αGSU (α-glycoprotein subunit), βTSH, βFSH, βLH, GH, PRL, and ACTH. Expression of mt1 mRNA was found in small round cells, co-localized with αGSU and βTSH. However, not all βTSH-containing cells expressed mt1 mRNA. The distribution of mt1- and RORβ-positive cells appeared to overlap, although more cells were labeled for RORβ than for mt1. Gonadotrophs, as well as other pars distalis cell types, were never labeled for mt1 melatonin receptor. Therefore, this study identifies the “specific” cells of the PT as the mt1 melatonin receptor-expressing cells.

scholarly journals Baihe Jizihuang Tang Ameliorates Chronic Unpredictable Mild Stress-Induced Depression-Like Behavior: Integrating Network Pharmacology and Brain-Gut Axis Evaluation

2021 ◽  
Vol 2021 ◽  
pp. 1-20
Author(s):  
Jian-ping Zhu ◽  
Hua-ying Wu ◽  
Yuan Zi ◽  
Xin-bin Xia ◽  
Meng-zhou Xie ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Hpa Axis ◽  
Hippocampal Neurons ◽  
Intestinal Flora ◽  
Network Pharmacology ◽  
Chemical Components ◽  
Mild Stress ◽  
Model Assessment ◽  
Chronic Unpredictable Mild Stress ◽  
16S Rna

Baihe Jizihuang Tang (BHT) is a traditional Chinese medicine (TCM) prescription, which can also be used as a nutritional food with medicinal value. Herein, we aimed to clarify the antidepressive effects and molecular mechanism of BHT. Network pharmacological analysis; chronic unpredictable mild stress (CUMS) rat model assessment; behavioral tests; analysis of hippocampal neurotransmitter levels, hippocampal pathological structure, and hypothalamic-pituitary-adrenal (HPA) axis; western blot analysis; 16s RNA sequencing; ultraperformance liquid chromatography (UPLC)/mass spectrometry (MS); and high-performance liquid chromatography (HPLC)/ultraviolet (UV) analysis were used. We found 8 potentially active components and 12 targets from the database. KEGG analysis suggested that BHT significantly affected BDNF/tyrosine receptor kinase B levels, glutamate binding, synaptic transmission based on neurotransmitter signal, and the response to glucocorticoid signaling pathways. Consistently, 7 chemical components were identified using UPLC/quadrupole time-of-flight/MS; among them, regalosides A, B, C, and E were unique components of lily of TCM, and their content in BHT was significantly different: regaloside A > B > E > C. BHT could nourish hippocampal neurons, affect neurotransmitter metabolism, reduce HPA axis hyperactivity, improve deficits in hippocampal tissue structure, and change depressive behavior. Moreover, BHT regulated BDNF expression in the hippocampus and improved intestinal flora deficits in CUMS rats by changing the content of Bifidobacterium, Rothia, Glutamicibacter, and Lactobacillus at the genus level. Collectively, BHT attenuated CUMS-induced depression-like behavior by regulating BDNF and intestinal flora disorder through the brain-gut axis. Therefore, including BHT in the medication list may constitute a potential strategy for preventing depression.

Melatonin receptor type 2 gene rs10830963 polymorphism and heart rate parameters in men with different types of physical activity

2020 ◽  
Vol 21 (3) ◽  
pp. 37-42
Author(s):  
S. N. Kolomeichuk ◽  
◽  
D. G. Gubin ◽  
N. Y. Prokopiev ◽  
◽  
...  
Keyword(s):  
Physical Activity ◽  
Heart Rate ◽  
Cardiovascular System ◽  
Receptor Gene ◽  
Marker Gene ◽  
Polymorphic Marker ◽  
Control Group ◽  
Melatonin Receptor ◽  
Healthy Donors ◽  
Different Types

Objective. The cardiovascular system demonstrates daily periodic changes in heart rate and blood pressure. Melatonin is involved in the regulation of the cardiovascular system by modulating sympathetic function and signaling pathways associated with nitric oxide (NO), as well as through interactions with its receptors. The aim of the study was to study the distribution of the allele frequency of the rs10830963 MTNR1B polymorphic marker gene in healthy donors, as well as to search for a relationship between variants of this gene and heart rate parameters in healthy donors with different types of exercise. Materials and methods. Study design – cross-sectional, non-randomized. The study included 80 healthy donors with various types of physical activity, as well as a control group made up of men leading a sedentary lifestyle. Venous blood samples from donors were used for DNA isolation. The polymorphism of the polymorphic marker rs10830963 of the MTNR1B type melatonin receptor gene was studied by PCR. Heart rate parameters were assessed using the Poly-Spectrum-8 / E software and hardware complex (Neurosoft, Russia) according to the generally accepted method. Results. Significantly low values of muscle mass in the control group are shown in comparison with donors of strength specialization. No associations were found between the polymorphic marker rs10830963 of the MTNR 1B gene and deviations from normal body weight and BMI, as well as parameters of heart rate variability in the studied donors. Conclusion. The rs10830963 polymorphic marker of the MTNR 1B gene is not associated with BMI and heart rate parameters of healthy donors. This sample of examined patients (less than 100 people) does not allow to reliably estimate the parameters of the heart rate in donors with a certain type of physical activity and carriage of a certain allele rs10830963 of the MTNR1B gene.

scholarly journals Proteomic Response of Rat Pituitary Under Chronic Mild Stress Reveals Insights Into Vulnerability and Resistance to Anxiety or Depression

2021 ◽  
Vol 12 ◽  
Author(s):  
Fenfang Tian ◽  
Dan Liu ◽  
Jin Chen ◽  
Wei Liao ◽  
Weibo Gong ◽  
...  
Keyword(s):  
Hpa Axis ◽  
Significant Risk ◽  
Regulatory System ◽  
Proteome Analysis ◽  
Chronic Mild Stress ◽  
Significant Risk Factor ◽  
Mild Stress ◽  
Protein Targets ◽  
Independent Analysis ◽  
Rat Pituitary

Chronic stress as one of the most significant risk factor can trigger overactivity of hypothalamic-pituitary-adrenal (HPA) axis in depression as well as anxiety. Yet, the shared and unique neurobiological underpinnings underlying the pituitary abnormality in these two disorders have not been made clear. We previously have established depression-susceptible, anxiety-susceptible and insusceptible groups using a valid chronic mild stress (CMS) model. In this work, the possible protein expression changes in the rat pituitary of these three groups were continuously investigated through the use of the comparative quantitative proteomics and bioinformatics approaches. The pituitary-proteome analysis identified totally 197 differential proteins as a CMS response. These deregulated proteins were involved in diverse biological functions and significant pathways potentially connected with the three different behavioral phenotypes, likely serving as new investigative protein targets. Afterwards, parallel reaction monitoring-based independent analysis found out that expression alterations in Oxct1, Sec24c, Ppp1cb, Dock1, and Coq3; Lama1, Glb1, Gapdh, Sccpdh, and Renbp; Sephs1, Nup188, Spp1, Prodh1, and Srm were specifically linked to depression-susceptible, anxiety-susceptible and insusceptible groups, respectively, suggesting that the same CMS had different impacts on the pituitary protein regulatory system. Collectively, the current proteomics research elucidated an important molecular basis and furnished new valuable insights into neurochemical commonalities and specificities of the pituitary dysfunctional mechanisms in HPA axis underlying vulnerability and resistance to stress-induced anxiety or depression.

scholarly journals Relationship between the Chemical Composition and the Biological Functions of Coffee

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7634
Author(s):  
Shah Saud ◽  
Ahmad Mohammad Salamatullah
Keyword(s):  
Chemical Composition ◽  
Blood Sugar ◽  
Biological Function ◽  
Biological Activities ◽  
Chemical Components ◽  
Biological Functions ◽  
Coffee Plant ◽  
Theoretical Support ◽  
The World ◽  
Further Development

Coffee is a Rubiaceae coffee plant ranked as the first of the three most important beverages in the world, with effects including lowering blood sugar, protecting the liver, and protecting the nerves. Coffee contains many chemical components, including alkaloids, phenolic acids, flavonoids, terpenoids, and so on. Chemical components in coffee are the basis of its biological function and taste. The chemical components are the basis of biological activities and form the characteristic aroma of coffee. The main chemical components and biological activities of coffee have been extensively studied, which would provide a relevant basis and theoretical support for the further development of the coffee industry.

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