Basal, luminal and myoepithelial cells: immunophenotypical and molecular characterization

AbstractDuring aging in the human mammary gland, luminal epithelial cells lose lineage fidelity by expressing markers normally expressed in myoepithelial cells. We hypothesize that loss of lineage fidelity is a general manifestation of epithelia that are susceptible to cancer initiation. In the present study, we show that histologically normal breast tissue from younger women who are susceptible to breast cancer, as a result of harboring a germline mutation in BRCA1, BRCA2 or PALB2 genes, exhibits hallmarks of accelerated aging. These include proportionately increased luminal epithelial cells that acquired myoepithelial markers, decreased proportions of myoepithelial cells and a basal differentiation bias or failure of differentiation of cKit+ progenitors. High-risk luminal and myoepithelial cells are transcriptionally enriched for genes of the opposite lineage, inflammatory- and cancer-related pathways. We have identified breast-aging hallmarks that reflect a convergent biology of cancer susceptibility, regardless of the specific underlying genetic or age-dependent risk or the associated breast cancer subtype.

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Abstract 3121: Study of leukemia inhibitory factor (LIF) system in a murine mammary tumor comprising luminal and myoepithelial cells

10.1158/1538-7445.am2011-3121 ◽

2011 ◽

Author(s):

Martin A. Krasnapolski ◽

Ana Quaglino ◽

Edith Kordon ◽

Elisa D. Bal de Kier Joffe

Keyword(s):

Mammary Tumor ◽

Leukemia Inhibitory Factor ◽

Myoepithelial Cells ◽

Inhibitory Factor ◽

Murine Mammary Tumor ◽

Luminal And Myoepithelial Cells

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Luminal MCF-12A and myoepithelial-like Hs 578Bst cells form bilayered acini similar to human breast

10.1101/248765 ◽

2018 ◽

Author(s):

Anne Weber-Ouellette ◽

Mélanie Busby ◽

Isabelle Plante

Keyword(s):

Cell Lines ◽

Smooth Muscle Actin ◽

Three Dimensional ◽

Myoepithelial Cells ◽

Normal Breast ◽

Breast Development ◽

Basal Cells ◽

Culture Model ◽

Luminal And Myoepithelial Cells

2.3.1AbstractThe mammary gland is a complex organ, structured in a ramified epithelium supported by the stroma. The epithelium’s functional unit is the bilayered acinus, made of a layer of luminal cells surrounded by a layer of basal cells mainly composed of myoepithelial cells. The aim of this study was to develop a reproducible and manipulable three-dimensional co-culture model of the bilayered acinus in vitro to study the interactions between the two layers. Two different combinations of cell lines were co-cultured in Matrigel: SCp2 and SCg6 mice cells, or MCF-12A and Hs 578Bst human cell lines. Cell ratios and Matrigel concentration were optimized. The resulting acini were analysed by confocal microscopy using epithelial (E-cadherin) and myoepithelial (α-smooth muscle actin) markers. SCp2 and SCg6 cells formed distinct three-dimensional structures, whereas MCF-12A and Hs 578Bst cells formed some bilayered acini. This in vitro bilayered acini model will allow us to understand the role of interactions between luminal and myoepithelial cells in the normal breast development.

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Characterization in vitro of luminal and myoepithelial cells isolated from the human mammary gland by cell sorting

Differentiation ◽

10.1111/j.1432-0436.1991.tb00883.x ◽

1991 ◽

Vol 46 (3) ◽

pp. 209-221 ◽

Cited By ~ 84

Author(s):

Michael J. O'Hare ◽

Michael G. Ormerod ◽

Paul Monaghan ◽

E. Birgitte Lane ◽

Barry A. Gusterson

Keyword(s):

Mammary Gland ◽

Cell Sorting ◽

Myoepithelial Cells ◽

Human Mammary Gland ◽

Luminal And Myoepithelial Cells

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The Mammary Gland Microenvironment Directs Progenitor Cell FateIn Vivo

International Journal of Cell Biology ◽

10.1155/2011/451676 ◽

2011 ◽

Vol 2011 ◽

pp. 1-11 ◽

Cited By ~ 12

Author(s):

Karen M. Bussard ◽

Gilbert H. Smith

Keyword(s):

Mammary Gland ◽

Epithelial Cell ◽

Cell Fate ◽

Progenitor Cell ◽

Myoepithelial Cells ◽

Mammary Epithelial ◽

Terminal End Buds ◽

Mammary Stem ◽

Luminal And Myoepithelial Cells

The mammary gland is a unique organ that continually undergoes postnatal developmental changes. In mice, the mammary gland is formed via signals from terminal end buds, which direct ductal growth and elongation. Intriguingly, it is likely that the entire cellular repertoire of the mammary gland is formed from a single antecedent cell. Furthermore, in order to produce progeny of varied lineages (e.g., luminal and myoepithelial cells), signals from the local tissue microenvironment influence mammary stem/progenitor cell fate. Data have shown that cells from the mammary gland microenvironment reprogram adult somatic cells from other organs (testes, nerve) into cells that produce milk and express mammary epithelial cell proteins. Similar results were found for human tumorigenic epithelial carcinoma cells. Presently, it is unclear how the deterministic power of the mammary gland microenvironment controls epithelial cell fate. Regardless, signals generated by the microenvironment have a profound influence on progenitor cell differentiationin vivo.

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Further Studies on the Ultrastructure of Harderian Gland in Adult Rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051451 ◽

1974 ◽

Vol 32 ◽

pp. 288-289

Author(s):

Alfredo Feria-Velasco ◽

Guadalupe Tapia-Arizmendi

Keyword(s):

Fine Structure ◽

Domestic Fowl ◽

Animal Species ◽

Acinar Cells ◽

Harderian Gland ◽

Myoepithelial Cells ◽

The Other ◽

Albino Rats ◽

Adult Rats ◽

Cell Components

The fine structure of the Harderian gland has been described in some animal species (hamster, rabbit, mouse, domestic fowl and albino rats). There are only two reports in the literature dealing on the ultrastructure of rat Harderian gland in adult animals. In one of them the author describes the myoepithelial cells in methacrylate-embbeded tissue, and the other deals with the maturation of the acinar cells and the formation of the secretory droplets. The aim of the present work is to analize the relationships among the acinar cell components and to describe the two types of cells located at the perifery of the acini.

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Ultrastructure of myoepithelial cell in parotid gland

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100103772 ◽

1988 ◽

Vol 46 ◽

pp. 342-343

Author(s):

C. N. Sun

Keyword(s):

Parotid Gland ◽

Osmium Tetroxide ◽

Individual Cell ◽

Branching Processes ◽

Muscle Cells ◽

Myoepithelial Cells ◽

Uranyl Acetate ◽

Thin Sections ◽

Gland Carcinoma ◽

Parotid Gland Carcinoma

Myoepithelial cells have been observed in the prostate, harderian, apocrine, exocrine sweat and mammary glands. Such cells and their numerous branching processes form basket-like structures around the glandular acini. Their shapes are quite different from structures seen either in spindleshaped smooth muscle cells or skeletal muscle cells. These myoepithelial cells lie on the epithelial side of the basement membrane in the glands. This presentation describes the ultrastructure of such myoepithelial cells which have been found also in the parotid gland carcinoma from a 45-year old patient.Specimens were cut into small pieces about 1 mm3 and immediately fixed in 4 percent glutaraldehyde in phosphate buffer for two hours, then post-fixed in 1 percent buffered osmium tetroxide for 1 hour. After dehydration, tissues were embedded in Epon 812. Thin sections were stained with uranyl acetate and lead citrate. Ultrastructurally, the pattern of each individual cell showed wide variations.

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An Ultrastructural Study of Pericytes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010010007x ◽

1968 ◽

Vol 26 ◽

pp. 66-67

Author(s):

T. M. Murad ◽

E. von Haam

Keyword(s):

Plasma Membrane ◽

Endothelial Cell ◽

Basement Membrane ◽

Blood Vessel ◽

Vascular Endothelial Cells ◽

Myoepithelial Cells ◽

Capillary Blood ◽

The Body ◽

Capillary Blood Vessel ◽

Outer Plasma Membrane

Pericytes are vascular satellites present around capillary blood vessels and small venules. They have been observed in almost every tissue of the body and are thought to be related to vascular smooth muscle cells. Morphologically pericytes have great similarity to vascular endothelial cells and also slightly resemble myoepithelial cells.The present study describes the ultrastructural morphology of pericytes in normal breast tissue and in benign tumor of the breast. The study showed that pericytes are ovoid or elongated cells separated from the endothelial cell of the capillary blood vessel by the basement membrane of endothelial cell. The nuclei of pericytes are often very distinctive. Although some are round, oval, or elongated, others show marked irregularity and infolding of the nuclear membrane. The cytoplasm shows mono-or bipolar extension in which the cytoplasmic organelles are located (Fig. 1). These cytoplasmic extensions embrace the capillary blood vessel incompletely. The plasma membrane exhibits multiple areas of focal condensation called hemidesmosomes (Fig. 2, arrow). A variable number of pinocytotic vesicles are frequently seen lining the outer plasma membrane. Normally pericytes are surrounded by a basement membrane which is found more consistently on the outer plasma membrane separating the pericytes from the stromal connective tissue.

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