Total phenol and flavonoid contents of extracts from different plant parts of Teucrium montanum L.

Annonamuricata plant parts possess a broad range of medicinal and biological properties. This research compared the chemical composition and antioxidant properties of Annonamuric at aparts. Proximate, mineral, total phenol and total flavonoid content as well as invitro antioxidant activity were examined. Results revealed the leaves contained significantly(p<0.05) higher composition of moisture (8.69±0.22%), ash (4.60±0.02%), protein (14.53±0.11%), crude fat (10.28±0.03%),chromium (0.38±0.05 mg/100g), nickel (1.75±0.04mg/100g), total phenol (1.01±0.03mgpyrocatechol/mL) and total flavonoid (1.12±0.03mgGAE/mL)compared to the respective values for root. Carbohydrate (9.29±0.24%), lead (0.13±0.02mg/100g) and cobalt (1.93±0.02mg/100g) composition was significantly lower(p<0.05) in the leaves compared to the respective compositions in the root. The leaf and root extract exhibited a concentration-dependent increase in hydroxyl radical scavenging activity with no observable(p<0.05)difference in their EC50 value. This study suggests the leaves of A. muricata found in Covenant University had better chemical composition when compared to the root. Nonetheless, these plant parts may be further exploited for not only their nutritive composition and mineral content but also a natural source of antioxidant agents.

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Insights into the bioactive compounds, antioxidant potential and TLC profiling of different extracts of Tomato plants

Jahangirnagar University Journal of Biological Sciences ◽

10.3329/jujbs.v7i2.40748 ◽

2018 ◽

Vol 7 (2) ◽

pp. 65-77 ◽

Cited By ~ 2

Author(s):

Sium Ahmed ◽

Faisal Bin Rahman ◽

Shawon Ahmed ◽

Abdullah Mohammad Shohael

Keyword(s):

Protein Content ◽

Bioactive Compounds ◽

Total Protein ◽

Tomato Plant ◽

Tomato Fruit ◽

Total Phenol ◽

Antioxidant Potential ◽

Total Protein Content ◽

Total Flavonoids ◽

Plant Parts

Tomato (Solanum lycopersicum L.) is a widely cultivated vegetable crop worldwide and its consumption is increasing day by day. The only edible part of tomato plant is its fruit. Therefore, the residual tomato plant parts are considered as waste after fruit collection despite some portion of it is used as livestock feed and in the production of fertilizer. Moreover, due to the consumption issue, much of the research is focused on tomato fruit rather than other plant parts. Bioactive compounds can be present in any part of the plant and can be isolated and recovered. Therefore, tomato plant may be useful as a source of bioactive compounds. Our study focuses on the phytochemical constituents and bioactive compounds that are present in different parts of the tomato plant that were being less studied before. Qualitative phytochemical tests were performed to identify the presence of different bioactive compounds. Saponins, tannins, glycosides, phenols, alkaloids, flavonoids and resins are present in significant amount. Total phenol, total flavonoids, total tannin, and total protein content were measured quantitatively. Leaf samples showed higher concentrations of bioactive compounds than roots and stem. Highest total phenol content (66.43 mg/g), total flavonoids content (28.00 mg/g), total tannin content (7.36 mg/g) and total protein content (26.55 mg/g) was found from the leaf water extract. DPPH scavenging assay was performed to find the antioxidant potential and positive results were found. Thin layer chromatography (TLC) was also employed to check the constituents. Our study found several spots for different samples and their retention factors (Rf) were evaluated. Jahangirnagar University J. Biol. Sci. 7(2): 65-77, 2018 (December)

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Studies on some Primary Metabolites Extraction and Quantification in Different Plant Parts of Eupatorium triplinerve Vahl.

International Letters of Chemistry Physics and Astronomy ◽

10.18052/www.scipress.com/ilcpa.49.66 ◽

2015 ◽

Vol 49 ◽

pp. 66-73

Author(s):

N. Sugumar ◽

S. Karthikeyan

Keyword(s):

Ascorbic Acid ◽

Chemical Composition ◽

Medicinal Plant ◽

Total Phenol ◽

World Population ◽

Chlorophyll B ◽

Primary Metabolites ◽

Plant Parts ◽

Life Saving ◽

The World

Medicinal plant is most exclusive sources of life saving drugs for the majority of the world population. Laboratory evaluations were made assess the study of primary metabolites of various plants in selected species Eupatorium triplinerve Vahl. During the present investigation comparative study of primary metabolites Carbohydrates, Protein, Ascorbic acid, Starch, Sugar, Chlorophyll, Carotenoids and Total phenol of genus Eupatorium triplinerve were undertaken for study and all the their chemical composition. The leaf highest amount of chlorophyll-a (4.95gm), chlorophyll-b (4.05gm), carotenoids (3.29gm),ascorbic acid (41.13gm/gdm), sugar (3.92gm/gdm), starch(5.89gm/gdm)protein (67.82µg/mg), total phenol (84.00gm/gdm). The root lowest amount of sugar (2.00gm/gdm), starch (3.15gm/gdm), protein (60.01µg/mg), ascorbic acid (43.09gm/gdm), total phenol (65.13gm/gdm).

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ANTIOXIDANT ACTIVITY AND TOTAL PHENOL CONTENT OF DIFFERENT PLANT PARTS OF LEBANESE ANNONA SQUAMOSA LINN.

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2020v12i8.36992 ◽

2020 ◽

pp. 100-105

Author(s):

FARAH IBRAHIM ◽

ALI JABER ◽

GHASSAN IBRAHIM ◽

EDMOND CHEBLE

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Total Phenol ◽

Free Radical Scavengers ◽

Total Phenol Content ◽

Phenol Content ◽

Annona Squamosa ◽

Plant Parts ◽

Ethanolic Extracts

Objective: The aim of this work was to characterize the antioxidant properties and to evaluate the total phenol content of leaves, bark, pericarp, and pulp extracts of Lebanese Annona squamosa Linn. (A. squamosa),, as well as a total screening of secondary metabolites present in the various plant parts studied. Methods: Two solvent systems were used for extraction: ethanol 80 % and methanol 80 %. The antioxidant activity of different extracts was investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The Total Phenol Content (TPC) of the different plant parts are determined and compared via Folin-Ciocalteu method. The results were presented as the mean of three separate experiments and error bars were used to illustrate standard deviation. Results: The phenolic content was found to be highest in the A. squamosa leaves methanolic and ethanolic extracts (117.2 mg and 112.92 gallic acid extract/g, respectively). The results showed that A. squamosa leaves methanolic and ethanolic extracts display the highest antioxidant activities than the bark, pulp and pericarp extracts, with half-maximal inhibitory concentration values 13.61 and 15.97 μg. ml-1 respectively. Ethanol 80 % and methanol 80 % were found to be efficient for the extraction of phenolic compounds. Conclusion: Results of this study indicate the presence of promising compounds in Lebanese A. squamosa that are able to act as antioxidants and free radical scavengers.

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Total phenol, flavonoid, individual phenolic acid contents, and antioxidant and cytotoxic activities of methanol extracts from Ficus cordata ssp. salicifolia

Canadian Journal of Plant Science ◽

10.1139/cjps-2020-0055 ◽

2021 ◽

Author(s):

Fatima Omari Alzahrani ◽

Abdelazim Ali Ahmed ◽

Sami Asir Al-Robai ◽

Haidar Abdalgadir Mohamed ◽

Mohamed Abdallah ◽

...

Keyword(s):

Antioxidant Activity ◽

Cytotoxic Activity ◽

Cell Lines ◽

Phenolic Acid ◽

Total Phenol ◽

Breast Adenocarcinoma ◽

Cytotoxic Activities ◽

Methanol Extracts ◽

Fruit Extracts ◽

Plant Parts

ABSTRACT This study aimed to evaluate the total phenol, flavonoid, and individual phenolic acid contents, and the antioxidant and cytotoxic activities of methanol extracts from <i>Ficus cordata</i> ssp. <i>salicifolia</i>. Spectrophotometry and gas chromatography-mass spectrometry (GC-MS) were used for chemical analyses, and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay was conducted to determine the antioxidant activity. The cytotoxic activity was assessed against MCF7 (human breast adenocarcinoma), A2780 (human ovary adenocarcinoma), HT29 (human colon adenocarcinoma), and MRC5 (normal human fetal lung fibroblast) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The total content of phenols, flavonoids, and antioxidant activity of the studied dry leaf, stem bark, twig, and fruit extracts were between 13.14 and 22.94 mg GAEg-1 DW, 8.03 and 12.72 mg QEg-1 DW, and 75.59% and 83.96%, respectively. Ten individual phenolic acids were quantified in the studied plant parts. The prominent phenolic acid was p-hydroxybenzoic acid, followed by vanillic acid. Methanolic fruit extracts exhibited promising cytotoxic activity against MCF7 (IC50: 21.11 µg mL-1). Additionally, A2780 cell lines responded very well to all the extracts, with an IC50 from 8.16–13.72 µg mL-1, except for the fruit extract, which was 47.39 µg mL-1. The leaf extract showed significant cytotoxicity against HT29 cell lines, with an IC50 of 8.81 µg mL-1. All extracts showed IC50 values ranging from 24.68 to 56.15 µg mL-1 on normal cells. Further examination of the plant parts in this study could identify compounds accounting for the observed antioxidant and cytotoxic activities and might represent potential novel anticancer drugs.

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