Antioxidant capacity and cytotoxic activity of essential oil of dry leaves from Guatteria blepharophylla (Mart.) R. E. Fries (Annonaceae)

In this study, an efficient method to enhance phenolic compound production in the in vitro cultured shoots of Polyscias filicifolia was developed. The phenolic compound content in P. filicifolia has not yet been reported. Shoots were treated with methyl jasmonate (JM) or salicylic acid (SA) at doses of 50, 100, or 200 µM. HPLC-UV-VIS and LC-MS techniques were used for the determination of chlorogenic, caffeic, and ferulic acids. The total phenolics and flavonoids were quantified, and the antioxidant capacity of plant extracts was determined using DPPH and ABTS methods. Finally, the cytotoxic activity of P. filicifolia extracts in normal (HaCaT) and cancer (A549) cells was investigated. Further, the effect of the extracts on cisplatin cytotoxicity was assessed.The elicitors significantly enhanced phenolic production compared to that in untreated shoots and leaves of intact plants. Chlorogenic acid was the most abundant compound with the highest yield of 5.03 ±0.25 mg/g DW after treatment with 50 µM SA. The total flavonoid and phenolic content was significantly and dose-dependently influenced by JM. The highest antioxidant capacity was noted in extracts derived from shoots grown on media supplemented with 50 µM SA and 200 µM JM; these doses were used for further cytotoxic activity investigations. The extracts from JM or SA treatments reduced cancer cell viability and increased their mortality, whereas the extract from JM treatment exhibited protective effect on normal cells. Moreover, the comparison of cytotoxic properties of plant extracts and cisplatin indicated that plant phenolic compounds in combination with anticancer drugs could reduce the detrimental effect of the latter on human cells.

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Essential oil composition and cytotoxic activity ofDucrosia anethifoliaandDucrosia flabellifoliafrom Iran

Journal of Essential Oil Research ◽

10.1080/10412905.2013.773656 ◽

2013 ◽

Vol 25 (2) ◽

pp. 160-163 ◽

Cited By ~ 13

Author(s):

S. Shahabipour ◽

O. Firuzi ◽

M. Asadollahi ◽

E. Faghihmirzaei ◽

K. Javidnia

Keyword(s):

Essential Oil ◽

Cytotoxic Activity ◽

Essential Oil Composition ◽

Oil Composition

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Thymol Chemotype Origanum vulgare L. Essential Oil as a Potential Selective Bio-Based Herbicide on Monocot Plant Species

Molecules ◽

10.3390/molecules25030595 ◽

2020 ◽

Vol 25 (3) ◽

pp. 595 ◽

Cited By ~ 4

Author(s):

Daniela Gruľová ◽

Lucia Caputo ◽

Hazem S. Elshafie ◽

Beáta Baranová ◽

Laura De Martino ◽

...

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Cytotoxic Activity ◽

Plant Species ◽

Pseudomonas Syringae ◽

Penicillium Expansum ◽

Chemical Pollution ◽

Origanum Vulgare ◽

Promising Source ◽

Dicot Species

Searching for new bio-based herbicides is crucial for decreasing chemical pollution, protecting the environment, and sustaining biodiversity. Origanum vulgare is considered a promising source of essential oil with herbicidal effect. The mode of action is not known. The present study focused on (1) comparison of phytotoxic activity of Origanum vulgare EO on monocot (Triticum aestivum and Hordeum vulgare) and dicot species (Lepidium sativum and Sinapis alba); (2) and evaluating other antimicrobial biological activities against phytopatogen bacteria (Clavibacter michiganensis, Pseudomonas syringae pv. phaseolicola, Pseudomonas savastanoi, and Xanthomonas campestris); antifungal activity against Monilinia fructicola, Aspergillus niger, Penicillium expansum, and Botrytis cinerea; cytotoxic activity and antioxidant activity. According to the GC/MS analyses, the EO belongs to the thymol chemotype O. vulgare with its high content of thymol (76%). Germination of all four species was not influenced by EO. The phytotoxic effect was statistically significant in the monocot species, while in the dicot species the opposite was observed—a stimulation effect, which was also statistically significant. Strong biological activity of O. vulgare EO was noted on all phytopatogen bacteria and fungi in the highest dose. Cytotoxic activity showed an IC50 = 50.5 μg/mL. Antioxidant activity showed an IC50 = 106.6 μg/mL after 45 min experimental time. Based on the presented results, it is possible to conclude that thymol chemotype O. vulgare essential oil could be potentially used as a herbicide with selective effects on monocot plant species.

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Composition and in-vitro CytotoxicActivities of the Leaf Essential Oil of Beilschmiedia erythrophloia from Taiwan

Natural Product Communications ◽

10.1177/1934578x1300800135 ◽

2013 ◽

Vol 8 (1) ◽

pp. 1934578X1300800

Author(s):

Yu-Chang Su ◽

Chen-Lung Ho

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Cytotoxic Activity ◽

Cancer Cells ◽

Cytotoxic Activities ◽

Anticancer Activities ◽

Leaf Essential Oil ◽

Main Components ◽

Type Apparatus

This study investigated the chemical composition and in-vitro cytotoxic activities of the essential oil isolated from the leaf of Beilschmiedia erythrophloia. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Fifty-five compounds were identified, representing 100% of the oil. The main components identified were β-caryophyllene (22.6%), α-humulene (21.9%), terpinen-4-ol (5.3%), cis-β-ocimene (5.1%), sabinene (5.0%) and limonene (4.5%). The anticancer activities of oil were evaluated. The results showed that the oil exhibited cytotoxic activity against human oral, liver, lung, colon, melanoma, and leukemic cancer cells.

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