Large-Scale Production of Intermediate-Purity, Soluble Human Plasminogen

1971 ◽  
Vol 25 (01) ◽  
pp. 105-113 ◽  
Author(s):  
K Bergström ◽  
Ann O. Tse ◽  
A. J Johnson
Keyword(s):  
Large Scale ◽  
Fresh Frozen Plasma ◽  
Scale Production ◽  
Plasmin Activity ◽  
Long Term Storage ◽  
Large Scale Production ◽  
Fresh Frozen ◽  
Human Plasminogen ◽  
Frozen Plasma

SummaryA method is described for laboratory, pilot or large-scale production of intermediatepurity human plasminogen soluble at neutral pH, with a specific proteolytic activity of approximately 12.5 CTA units/mg protein. Batches of 100-200 g can be readily produced from Fraction III paste from outdated or fresh-frozen plasma. Since this plasminogen is stable on long-term storage, and low in spontaneous plasmin activity, it is especially useful as an assay reagent (substrate) and as starting material for the preparation of high-purity plasminogen.

scholarly journals ACTIVITIES OF COAGULATION FACTORS IN FRESH FROZEN PLASMA AFTER LONG-TERM STORAGE

2016 ◽  
Vol 62 (4) ◽  
pp. 545-551
Author(s):  
Akihiro Fuchizaki ◽  
Jumpei Mori ◽  
Akira Iwama ◽  
Masayuki Shiba ◽  
Yu Naito ◽  
...  
Keyword(s):  
Coagulation Factors ◽  
Fresh Frozen Plasma ◽  
Long Term Storage ◽  
Fresh Frozen ◽  
Term Storage ◽  
Frozen Plasma

scholarly journals Low-cost Large Scale Vermicompost Unit

2021 ◽  
Vol 2115 (1) ◽  
pp. 012026
Author(s):  
Sonam Solanki ◽  
Gunendra Mahore
Keyword(s):  
Large Scale ◽  
Low Cost ◽  
Small Scale ◽  
Scale Production ◽  
Key Innovation ◽  
Main Challenge ◽  
Long Run ◽  
Large Scale Production ◽  
Term Maintenance

Abstract In the current process of producing vermicompost on a large-scale, the main challenge is to keep the worms alive. This is achieved by maintaining temperature and moisture in their living medium. It is a difficult task to maintain these parameters throughout the process. Currently, this is achieved by building infrastructure but this method requires a large initial investment and long-run maintenance. Also, these methods are limited to small-scale production. For large-scale production, a unit is developed which utilises natural airflow with water and automation. The main aim of this unit is to provide favourable conditions to worms in large-scale production with very low investment and minimum maintenance in long term. The key innovation of this research is that the technology used in the unit should be practical and easy to adopt by small farmers. For long-term maintenance of the technology lesser number of parts are used.

scholarly journals Fresh frozen plasma as a source of plasminogen for ligneous conjunctivitis: Case report and a review of the literature

2021 ◽  
pp. 031-035
Author(s):  
Park Benjamin J ◽  
Camoriano David ◽  
Vital Mark C ◽  
Chévez-Barrios Patricia ◽  
Goosey John D ◽  
...  
Keyword(s):  
Case Report ◽  
Fresh Frozen Plasma ◽  
Term Treatment ◽  
Ligneous Conjunctivitis ◽  
Caucasian Girl ◽  
Long Term Treatment ◽  
Effective Option ◽  
Fresh Frozen ◽  
Frozen Plasma

Purpose: To describe management of a patient with ligneous conjunctivitis secondary to plasminogen deficiency and review the literature on treatment with plasminogen. Design: Interventional case report. Methods: A 6-year-old Caucasian girl developed ligneous conjunctivitis recalcitrant to debridement and treatment with topical cyclosporin A and steroids. The literature was reviewed regarding treatment of ligneous conjunctivitis with plasminogen. Results: The patient was treated with plasminogen containing drops derived from her father’s fresh frozen plasma. The ligneous conjunctivitis resolved, and the patient was managed on these drops for the following 8 years, initiating treatment for flares and tapering according to clinical response. Conclusions: Plasminogen drops concentrated from fresh frozen plasma are a safe and effective option for long-term treatment of ligneous conjunctivitis.

scholarly journals Large-Scale Production of Lentiviral Vectors: Current Perspectives and Challenges

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1051
Author(s):  
Eduardo Martínez-Molina ◽  
Carlos Chocarro-Wrona ◽  
Daniel Martínez-Moreno ◽  
Juan A. Marchal ◽  
Houria Boulaiz
Keyword(s):  
Viral Vectors ◽  
Large Scale ◽  
Lentiviral Vectors ◽  
Good Manufacturing Practice ◽  
Scale Production ◽  
Gene Therapies ◽  
Packaging Cell ◽  
Large Scale Production ◽  
Target Production

Lentiviral vectors (LVs) have gained value over recent years as gene carriers in gene therapy. These viral vectors are safer than what was previously being used for gene transfer and are capable of infecting both dividing and nondividing cells with a long-term expression. This characteristic makes LVs ideal for clinical research, as has been demonstrated with the approval of lentivirus-based gene therapies from the Food and Drug Administration and the European Agency for Medicine. A large number of functional lentiviral particles are required for clinical trials, and large-scale production has been challenging. Therefore, efforts are focused on solving the drawbacks associated with the production and purification of LVsunder current good manufacturing practice. In recent years, we have witnessed the development and optimization of new protocols, packaging cell lines, and culture devices that are very close to reaching the target production level. Here, we review the most recent, efficient, and promising methods for the clinical-scale production ofLVs.

scholarly journals Argan Oil, Functional Food, and the Sustainable Development of the Argan Forest

2008 ◽  
Vol 3 (2) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Zoubida Charrouf ◽  
Dominique Guillaume
Keyword(s):  
Functional Food ◽  
Large Scale ◽  
Economic Value ◽  
Developed Countries ◽  
Scale Production ◽  
The Sustainable Development ◽  
Argan Oil ◽  
Large Scale Production ◽  
Argan Tree

For years, in southwestern Morocco, the decline of the argan forest has been accompanied by the concomitant desert encroachment. Preservation of this forest by increasing the economic value of argan tree was proposed twenty years ago, but successful large scale production of certified, high quality argan oil, an edible oil introduced as a functional food, has only been recently achieved. Argan oil is now marketed in most developed countries, despite its elevated price, and protection of the argan forest is now seriously being considered. The aim of this work is to present the recent progress made in argan oil production, the ways explored to commercialize the oil extraction by-products, and recent attempts to use other argan tree parts as part of a long term aim to preserve the argan forest.

scholarly journals ARTIFICIAL FOREST ECOSYSTEMS: CONDITION AND DEVELOPMENT PROSPECTS

2018 ◽  
Vol 13 (3) ◽  
pp. 39-45
Author(s):  
Раис Минниханов ◽  
Rais Minnihanov ◽  
Харрис Мусин ◽  
Kharris Musin ◽  
Нурсиль Гибадуллин ◽  
...  
Keyword(s):  
Large Scale ◽  
Forest Ecosystems ◽  
Scale Production ◽  
Good Seed ◽  
Clear Cutting ◽  
Large Scale Production ◽  
Soil Preparation ◽  
The Republic ◽  
Development Prospects

The research methodology is based on generally accepted silvicultural and taxation techniques, methods of observation, and the method of test plots. The objects of research were the forests of the Republic of Tatarstan. Forests were created on an area of 17 553 hectares with a clear cutting area of 9 190 hectares for the years 1962-2001 in the forest fund of the GKU “Sabinskoe Forestry”. A part of forest cultures was created under the canopy of soft-leaved stands, in which non-continuous logging was carried out, but they did not have a sufficient amount of conifer undergrowth. The number of self-seeding per 1 m2 of mineralized area with the disc soil mineralization of KLB-1.7 on average is 1.4 times more than in the control variant. The bulk of self-seeding settles in the middle of the prepared lanes and grounds. In 1990, the number of self-seeding at the center of the bands averaged 81%, and at the edges - 19%, with fluctuations in some areas from 51 to 85%. In the next 2 years, the number of self seeding at the center of the lanes decreased on average by 65%, at the edges to 97%. Consequently, in high-grade crops of spruce, one cannot count on the accumulation of self-seeding after sampling either every 4th or every 8th rows of crops and soil mineralization even with good seed yield. It is necessary to improve the method of cutting and soil preparation. The thinning of plantations in the adjacent rows at the same time as felling could be effective, but they were not considered in further studies, because clear felling showed its futility. Conducted long-term large-scale production experiments demonstrate the possibilities of transferring artificial forest ecosystems to the path of natural development by the system of complex logging.

scholarly journals Long-term audit of the use of fresh frozen plasma in a university hospital

2017 ◽  
Vol 12 (5) ◽  
pp. 437-444 ◽  
Author(s):  
Abdel Galil M. Abdel Gader ◽  
Abeer K. Al-Ghumlas ◽  
Abdul Kareem M. Al Momen ◽  
Sitel Banat A. Awadalla ◽  
Motasim Badri
Keyword(s):  
Fresh Frozen Plasma ◽  
University Hospital ◽  
Fresh Frozen ◽  
Frozen Plasma

scholarly journals Synthesis of Caffeoyl-Prolyl-Histidyl-Xaa Derivatives and Evaluation of Their Activities and Stability upon Long-Term Storage

2021 ◽  
Vol 22 (12) ◽  
pp. 6301
Author(s):  
Hyeri Jeong ◽  
Young-Eun Jeon ◽  
Jin-Kyoung Yang ◽  
Jaehi Kim ◽  
Woo-Jae Chung ◽  
...  
Keyword(s):  
Large Scale ◽  
Antioxidative Activity ◽  
Solid Phase ◽  
Critical Role ◽  
Radical Scavenging ◽  
Scale Production ◽  
Ambient Conditions ◽  
Long Term Storage ◽  
Term Storage

Antioxidants play a critical role in the treatment of degenerative diseases and delaying the aging of dermal tissue. Caffeic acid (CA) is a representative example of the antioxidants found in plants. However, CA is unsuitable for long-term storage because of its poor stability under ambient conditions. Caffeoyl-Pro-His-NH2 (CA-Pro-His-NH2, CA-PH) exhibits the highest antioxidant activity, free radical scavenging and lipid peroxidation inhibition activity among the histidine-containing CA-conjugated dipeptides reported to date. The addition of short peptides to CA, such as Pro-His, is assumed to synergistically enhance its antioxidative activity. In this study, several caffeoyl-prolyl-histidyl-Xaa-NH2 derivatives were synthesized and their antioxidative activities evaluated. CA-Pro-His-Asn-NH2 showed enhanced antioxidative activity and higher structural stability than CA-PH, even after long-term storage. CA-Pro-His-Asn-NH2 was stable for 3 months, its stability being evaluated by observing the changes in its NMR spectra. Moreover, the solid-phase synthetic strategy used to prepare these CA-Pro-His-Xaa-NH2 derivatives was optimized for large-scale production. We envision that CA-Pro-His-Xaa-NH2 derivatives can be used as potent dermal therapeutic agents and useful cosmetic ingredients.

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