scholarly journals Synthesis of Caffeoyl-Prolyl-Histidyl-Xaa Derivatives and Evaluation of Their Activities and Stability upon Long-Term Storage

2021 ◽  
Vol 22 (12) ◽  
pp. 6301
Author(s):  
Hyeri Jeong ◽  
Young-Eun Jeon ◽  
Jin-Kyoung Yang ◽  
Jaehi Kim ◽  
Woo-Jae Chung ◽  
...  
Keyword(s):  
Large Scale ◽  
Antioxidative Activity ◽  
Solid Phase ◽  
Critical Role ◽  
Radical Scavenging ◽  
Scale Production ◽  
Ambient Conditions ◽  
Long Term Storage ◽  
Term Storage

Antioxidants play a critical role in the treatment of degenerative diseases and delaying the aging of dermal tissue. Caffeic acid (CA) is a representative example of the antioxidants found in plants. However, CA is unsuitable for long-term storage because of its poor stability under ambient conditions. Caffeoyl-Pro-His-NH2 (CA-Pro-His-NH2, CA-PH) exhibits the highest antioxidant activity, free radical scavenging and lipid peroxidation inhibition activity among the histidine-containing CA-conjugated dipeptides reported to date. The addition of short peptides to CA, such as Pro-His, is assumed to synergistically enhance its antioxidative activity. In this study, several caffeoyl-prolyl-histidyl-Xaa-NH2 derivatives were synthesized and their antioxidative activities evaluated. CA-Pro-His-Asn-NH2 showed enhanced antioxidative activity and higher structural stability than CA-PH, even after long-term storage. CA-Pro-His-Asn-NH2 was stable for 3 months, its stability being evaluated by observing the changes in its NMR spectra. Moreover, the solid-phase synthetic strategy used to prepare these CA-Pro-His-Xaa-NH2 derivatives was optimized for large-scale production. We envision that CA-Pro-His-Xaa-NH2 derivatives can be used as potent dermal therapeutic agents and useful cosmetic ingredients.

Diapause induction and termination in Indian strains of Trichogramma chilonis (Hymenoptera: Trichogrammatidae)

2017 ◽  
Vol 149 (5) ◽  
pp. 607-615 ◽  
Author(s):  
Enakshi Ghosh ◽  
Chandish R. Ballal
Keyword(s):  
Large Scale ◽  
Biocontrol Agent ◽  
Storage Temperature ◽  
Adult Emergence ◽  
Diapause Induction ◽  
Storage Duration ◽  
Trichogramma Chilonis ◽  
Long Term Storage ◽  
Term Storage

AbstractThe role of temperature in diapause induction was studied as a mode of long-term storage of Trichogramma chilonis (Ishii) (Hymenoptera: Trichogrammatidae). Three different strains of this widely used biocontrol agent (T. chilonis Nilgiris strain, T. chilonis Kodaikanal strain, and T. chilonis 15 °C strain) reared on the factitious host Corcyra cephalonica (Stainton) (Lepidoptera: Pyralidae) were used for this experiment. Except T. chilonis laboratory strain, all the other strains could successfully undergo diapause at their pre-pupal stage. Maximum percentage of healthy pre-pupae were recorded in the three strains by providing a pre-storage temperature of 10 °C for 35 days with eight hours of photophase wherein 75–87% could enter into diapause. Further, at a maintenance temperature of 5 °C with 24 hours of scotophase, diapause could be maintained. Diapause could be terminated after six months of storage with 23–36% of adult emergence. However, there was significant reduction in longevity and parasitism rate of the emerged adults. Considering superior biological parameters, 95 days of storage (including pre-storage duration) could provide around 60% adult emergence. Successful long-term storage of T. chilonis strains through diapause induction can facilitate commercial insectaries in stockpiling this biocontrol agent for large-scale field releases. This is the first study on successful induction and termination of diapause in T. chilonis strains and evaluating their performance attributes.

scholarly journals The effect of pre-analytical conditions on blood metabolomics in epidemiological studies

2019 ◽  
Author(s):  
Diana L Santos Ferreira ◽  
Hannah J Maple ◽  
Matt Goodwin ◽  
Judith S Brand ◽  
Vikki Yip ◽  
...  
Keyword(s):  
Amino Acids ◽  
Large Scale ◽  
Epidemiological Studies ◽  
Storage Conditions ◽  
Long Term Storage ◽  
Edta Plasma ◽  
Beta Hydroxybutyrate ◽  
The Impact ◽  
Term Storage

AbstractBackgroundSerum and plasma are commonly used biofluids for large-scale metabolomic-epidemiology studies. Their metabolomic profile is susceptible to changes due to variability in pre-analytical conditions and the impact of this is unclear.MethodsParticipant-matched EDTA-plasma and serum samples were collected from 37 non-fasting volunteers and profiled using a targeted nuclear magnetic resonance (NMR) metabolomics platform (N=151 traits). Metabolic concentrations were compared between reference (pre-storage: 4°C, 1.5h; post-storage: no sample preparation or NMR-analysis delays) and four, pre-storage, blood processing conditions, where samples were incubated at (i) 4°C, 24h; (ii) 4°C, 48h; (iii) 21°C, 24h; (iv) 21°C, 48h, before centrifugation; and two, post-storage, sample processing conditions in which samples (i) thawed overnight, then left for 24h before addition of sodium buffer followed by immediate NMR analysis; (ii) thawed overnight, addition of sodium buffer, then left for 24h before profiling. Linear regression models with random-intercepts were used to assess the impact of these six pre-analytical conditions on EDTA-plasma/serum metabolome.ResultsFatty acids, beta-hydroxybutyrate, glycoprotein-acetyls and most lipid-related traits, in serum and plasma, were robust to the tested pre and post-storage conditions. Pre-storage conditions impacted concentrations of glycolysis metabolites, acetate, albumin and amino-acids by levels that could potentially bias research results (up to 1.4SD difference compared with reference). Post-storage conditions affected histidine, phenylalanine and LDL-particle-size, with differences up to 1.4SD.ConclusionsMost metabolic traits are robust to the pre- and post-storage conditions tested here and that may commonly occur in large-scale cohorts. However, concentrations of glycolysis metabolites, and amino-acids may be compromised.Key messagesIn large scale epidemiological studies, blood processing delays, incubation at high temperature prior to long term storage, and NMR profiling delays after long term storage, may occur.Concentrations of fatty acids, beta-hydroxybutyrate, glycoprotein acetyls and most lipid-related traits are robust to variations in pre-storage temperature and duration of incubation (4°C or 21°C for up to 48h prior to centrifugation) and post-storage sample handling (24h delay in sample preparation or NMR profiling).Glycolytic metabolite concentrations are altered by pre-storage conditions and amino-acids, particularly histidine and phenylalanine, by both, pre and post-storage conditions.

Large-Scale Production of Intermediate-Purity, Soluble Human Plasminogen

1971 ◽  
Vol 25 (01) ◽  
pp. 105-113 ◽  
Author(s):  
K Bergström ◽  
Ann O. Tse ◽  
A. J Johnson
Keyword(s):  
Large Scale ◽  
Fresh Frozen Plasma ◽  
Scale Production ◽  
Plasmin Activity ◽  
Long Term Storage ◽  
Large Scale Production ◽  
Fresh Frozen ◽  
Human Plasminogen ◽  
Frozen Plasma

SummaryA method is described for laboratory, pilot or large-scale production of intermediatepurity human plasminogen soluble at neutral pH, with a specific proteolytic activity of approximately 12.5 CTA units/mg protein. Batches of 100-200 g can be readily produced from Fraction III paste from outdated or fresh-frozen plasma. Since this plasminogen is stable on long-term storage, and low in spontaneous plasmin activity, it is especially useful as an assay reagent (substrate) and as starting material for the preparation of high-purity plasminogen.

A Practical, Large-Scale Synthesis of p-(Difluoroiodo)toluene (p-TolIF2)

Synthesis ◽  
2019 ◽  
Vol 51 (16) ◽  
pp. 3055-3059 ◽  
Author(s):  
Jason Tao ◽  
Graham K. Murphy
Keyword(s):  
Large Scale ◽  
High Quality ◽  
Elemental Fluorine ◽  
Fluorinating Agent ◽  
Long Term Storage ◽  
Quality Product ◽  
Large Scale Synthesis ◽  
Term Storage ◽  
High Quality Product

p-(Difluoroiodo)toluene (p-TolIF2) is a versatile fluorinating agent that acts as both a surrogate for elemental fluorine, and as a source of ‘electrophilic’ fluorine. Described here is a detailed three-step synthesis of p-TolIF2, carried out on a 50 mmol scale, that consistently provides high-quality product that is suitable for long-term storage. The reactions employ inexpensive, readily available starting materials and reagents, and uses the commodity chemical 48% aqueous HF as the source of fluorine atoms.

scholarly journals Morphology and Chemical Purity of Water Suspension of Graphene Oxide FLAKES Aged for 14 Months in Ambient Conditions. A Preliminary Study

Materials ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4108
Author(s):  
Adrian Chlanda ◽  
Krystian Kowiorski ◽  
Marcin Małek ◽  
Ewa Kijeńska-Gawrońska ◽  
Monika Bil ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Chemical Properties ◽  
Ambient Conditions ◽  
Long Term Storage ◽  
Graphene Derivatives ◽  
Nanoscale Characterization ◽  
Chemical Purity ◽  
Preliminary Study ◽  
Term Storage

Graphene and its derivatives have attracted scientists’ interest due to their exceptional properties, making them alluring candidates for multiple applications. However, still little is known about the properties of as-obtained graphene derivatives during long-term storage. The aim of this study was to check whether or not 14 months of storage time impacts graphene oxide flakes’ suspension purity. Complementary micro and nanoscale characterization techniques (SEM, AFM, EDS, FTIR, Raman spectroscopy, and elemental combustion analysis) were implemented for a detailed description of the topography and chemical properties of graphene oxide flakes. The final step was pH evaluation of as-obtained and aged samples. Our findings show that purified flakes sustained their purity over 14 months of storage.

Documentation and Digital Preservation of Syrian Heritage

2018 ◽  
Vol 40 (4) ◽  
pp. 107-128 ◽  
Author(s):  
Karin Pütt
Keyword(s):  
Built Environment ◽  
Cultural Heritage ◽  
Large Scale ◽  
Digital Archive ◽  
Islamic Art ◽  
Digital World ◽  
Long Term Storage ◽  
The Face ◽  
Term Storage

Since 2013, the Museum of Islamic Art in Berlin and the German Archeological Institute (DAI) have hosted a digital archive documenting Syrian heritage and built environment. This project was developed in the face of a war that would destroy places of cultural heritage on a large scale. The archive consists of photos, plans, and documents and includes more than 200,000 items. For long-term storage and attainability they are integrated into the digital world of the DAI. In order to raise awareness and to present the data to a wider audience, selected photos and drawings are bundled into stories on a new website with text in English, Arabic, and German. The project exhibits Syrian cultural heritage in both its tangible and nontangible aspects.

REPLENISHMENT OF AN APPLE TREE ASSORTMENT WITH DEEP WINTER VARIETIES

1970 ◽  
pp. 31-33
Author(s):  
Е. N. Sedov ◽  
T. V. Yanchuk ◽  
S. А. Korneeva
Keyword(s):  
Large Scale ◽  
The State ◽  
State Testing ◽  
Crop Breeding ◽  
State Register ◽  
Long Term Storage ◽  
Russian Research Institute ◽  
Apple Cultivars ◽  
Term Storage

Large-scale and purposeful work on the development of new apple cultivars has been carried out at Russian Research Institute of Fruit Crop Breeding since 1956. Over a long period of time, 55 apple cultivars of different fruit maturation periods were created and included in the state register of breeding achievements approved for use (zoned). Apple cultivars with fruits capable of long-term storage in refrigerator are of particular interest. Cultivars fruits of which can store until mid-March or longer include zoned cultivars: Aleksandr Boiko, Den Pobedy, Kulikovskoe, Ministr Kisilev, Sinap Orlovsky, as well as Zhdanovskoe, which is passing state testing. The article provides a brief description of six cultivars and a more complete characteristic of new cultivar Zhdanovskoe. Triploid cultivar Sinap Orlovsky is included in the state register in four regions of Russia, Kulikovskoe – in three regions, Svezhest - in two regions while Aleksandr Boiko, Den Pobedy, Kulikovskoe and Ministr Kisilev are included in the state register only in the Central Chernozem region. Zhdanovskoe, which is passing state testing, is also of interest for industry. Its fruit are stored in the refrigerator till mid-March. Among the listed cultivars there are 5 triploid cultivars, including 2 triploid cultivars that are immune to scab. It is known that triploid cultivars are prone to more regular fruiting over the years and have larger and more marketable fruits, and the introduction of scab-immune cultivars into widespread production improves the environmental situation in the orchard and provides cleaner products. The primary task of breeding institutions and production is to give these cultivars a comprehensive assessment.

Sign in / Sign up

Export Citation Format

Share Document