Metabolomics, genetics and epigenetics analysis of European yew for the selection of antineoplastic taxol/taxane producing plant material in Greece

2019 ◽  
Author(s):  
FA Aravanopoulos ◽  
EV Avramidou ◽  
A Xanthopoulou ◽  
E Dalmaris
Plant Disease ◽  
1999 ◽  
Vol 83 (5) ◽  
pp. 482-485 ◽  
Author(s):  
Margaret J. Green ◽  
Dan A. Thompson ◽  
Donald J. MacKenzie

A simple and efficient procedure for the extraction of high-quality DNA from phytoplasma-infected woody and herbaceous plants for polymerase chain reaction (PCR) detection is described. This procedure does not require phenol, chloroform, or alcohol for the precipitation of nucleic acids. Herbaceous and woody plant material are extracted in an identical manner with no additional purification or enrichment steps required. The method utilizes commercially available microspin-column matrices, and the extraction of total DNA can be achieved in less than 1 h. The method has been used to successfully purify phytoplasma DNA from whole leaves, leaf petioles and midribs, roots, and dormant wood from a diverse selection of plant material. The phytoplasmas detected by PCR include pear decline, western X-disease, peach yellow leaf roll, peach rosette, apple proliferation, Australian grapevine yellows, and Vaccinium witches'-broom.


2017 ◽  
Vol 45 (1) ◽  
pp. 1-8
Author(s):  
Gerland Akhmadi ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Desta Wirnas

Anther culture technique is able to accelerate plant breeding activities. The objectives of this research was to determine the agronomic traits that could be used for selection of the dihaploid rice line population through the calculation of heritability, genotypic correlation, path analysis and selecting dihaploid rice lines. The plant material used was 65 dihaploid rice lines DH 1 of F1 anther culture plants and Ciherang and Inpari 13 as check varieties. Agronomic characters that could be used as selection character are generative plant height, number of filled grain per panicle, the total number of grains per panicle, weight of 1,000 grains, and grain per hill. Twenty three dihaploid lines were selected based good agronomic characters with criteria generative plant height between 80-120 cm, number of filled grains per panicle > 100, number of grains per panicle > 120, weight of 1,000 grains > 20 g, and grain per hill > 25 g.Keywords: anther culture, heritability, agronomic characters


2015 ◽  
Vol 26 (1) ◽  
pp. 5-15
Author(s):  
E. Kuta ◽  
C. Ryka

The aim of the present paper Was to test the value of various plant extracts (yeast barm, mature walnut embryos, coconut meal, young spinach leaves and tomato juice) used as the complementary elements of White's mineral nutrient for the culture <i>in vitro</i> of generative apices of winter wheat, rape and wetch. The results showed that the generative apices of investigated planits had various nutritive requirements which point to the necessity of a careful selection of a specific nutrient for particular plant material.


Author(s):  
N. Triapitsyna ◽  
K. Udovychenko ◽  
S. Vasyuta

The results are the work during 2004—2018 years in Ukraine on the selection of material of stone fruits crops, which satisfies the requirements of the normative international documents for the creation of a prebasic clones collection. Analyzed state of plant material certification of these cultures.


Radiocarbon ◽  
2014 ◽  
Vol 56 (02) ◽  
pp. 679-690
Author(s):  
V Caracuta ◽  
G Fiorentino ◽  
M Turchiano ◽  
G Volpe

Charred plant materials found in archaeological contexts are usually considered the most reliable remains for radiocarbon dating. Usually, seeds and fruits are preferred to wood fragments because their short lifecycle reduces the range of uncertainty of the14C measurement. A selection of short-lived samples, mainly from barley and wheat, from the Late Roman site of Faragola (SE Italy) were14C dated; however, the14C dates obtained were not always consistent with the chronology provided by other archaeological evidence. A careful analysis of all the macrobotanical remains found in each of the dated contexts provides insight into the origin of the plant material, helping to distinguish betweenin situand non-in situmaterial. The14C dates are reconsidered in the context of findings and the kind of material selected for dating. Using the archaeological context, a Bayesian model was employed to reduce the range of the calibrated date and thereby refine the chronology of the site on an absolute basis.


2021 ◽  
Vol 22 (21) ◽  
pp. 11392
Author(s):  
Paweł Śliwa ◽  
Karolina Śliwa

The selection of the appropriate extraction method is crucial, especially for the receiving of active substances from plant material. The extraction using supercritical liquids and micellar-mediated extraction (MME) is the most advantageous among the alternative methods to classical solid–liquid extraction. However, the latter seems to be the best solution when the desired actives are polar. The following article presents a comprehensive review of the micellar-mediated extraction method in the last decade. The theoretical principle of the process was also refreshed and the current state of knowledge on the applications for analytical and manufacturing purposes was summarized.


Radiocarbon ◽  
2014 ◽  
Vol 56 (2) ◽  
pp. 679-690 ◽  
Author(s):  
V Caracuta ◽  
G Fiorentino ◽  
M Turchiano ◽  
G Volpe

Charred plant materials found in archaeological contexts are usually considered the most reliable remains for radiocarbon dating. Usually, seeds and fruits are preferred to wood fragments because their short lifecycle reduces the range of uncertainty of the14C measurement. A selection of short-lived samples, mainly from barley and wheat, from the Late Roman site of Faragola (SE Italy) were14C dated; however, the14C dates obtained were not always consistent with the chronology provided by other archaeological evidence. A careful analysis of all the macrobotanical remains found in each of the dated contexts provides insight into the origin of the plant material, helping to distinguish betweenin situand non-in situmaterial. The14C dates are reconsidered in the context of findings and the kind of material selected for dating. Using the archaeological context, a Bayesian model was employed to reduce the range of the calibrated date and thereby refine the chronology of the site on an absolute basis.


Author(s):  
Magdalena Gantner ◽  
Eugenia Czernyszewicz ◽  
Klaudia Kopczyńska ◽  
Katarzyna Król

The aim of the study is to compare the quality of hazelnuts grown in Poland in opposition to imported ones, based on their changes in physicochemical properties depending on the roasting parameters. The plant material was two hazelnuts cultivars grown in Poland: ‘Olbrzym z Halle’ and ‘Cosford’. The samples were roasted at nine specific temperatures and time conditions: at 110°C, 130°C and 160°C, each at 15, 30 and 60 min. Parameters such as water activity, color of the inner (brown) skin of the husk and color of the pure kernel’s surface and hardness were investigated. The roasting process reduced water activity, decreased luminosity (L*) of kernel hazelnuts irrespective of cultivar and increased browning index (BI) value. The main parameter determining these changes was temperature. In addition, as the process temperature increased the hardness of the nuts and their structure became more fragile and delicate. Nuts of ‘Cosford’ cultivar were characterized by higher hardness compared to the nuts of ‘Olbrzym z Halle’ cultivar. The temperature of 130° for 30 min provided the best quality of the roasted nuts, regardless of the cultivar. Nuts of both cultivars, roasted in these parameters were characterized by comparable, high ease of removal of the brown inner skin. The selection of optimal parameters of technological process can improve the processing properties of Polish hazelnuts and, consequently, ensure their competitive advantage against nuts grown in other countries. This fact could increase both the chance of their export as well as the use in Polish confectionery production.


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