scholarly journals A Study on the Serum γ-Glutamyltranspeptidase and Plasma Osteopontin in Alcoholic Liver Disease

2021 ◽  
Author(s):  
Arpita Suri ◽  
Naveen Singh ◽  
Sanjiv Kumar Bansal
Keyword(s):  
Liver Disease ◽  
Alcoholic Liver Disease ◽  
Cell Injury ◽  
Liver Function Tests ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Subjects ◽  
Serum Aspartate Aminotransferase ◽  
Liver Cell Injury ◽  
Positive Correlations ◽  
And Gender

Abstract Background Alcoholic liver disease (ALD) is a major source of alcohol-related morbidity and mortality. Heavy drinkers and alcoholics may progress from fatty liver to alcoholic hepatitis to cirrhosis. The enzyme γ-glutamyltranspeptidase (GGT) is a membrane-bound glycoprotein which catalyzes the transfer of the γ-glutamyl group from γ-glutamyl peptides to other peptides, amino acids, and water. Serum GGT activity mainly attributed to hepatobiliary system and thus is an important marker of ALD. Hence the present study is conducted to estimate and correlate the levels of GGT and osteopontin (OPN) in ALD. Aims and Objectives The objective of this study is to estimate and correlate the levels of GGT and OPN in ALD. Materials and Methods Sixty clinically diagnosed cases of ALD and sixty age- and gender-matched healthy controls were recruited for the study. Blood samples were collected from them and serum aspartate aminotransferase, serum alanine transaminases (ALTs), serum ALP levels, and plasma OPN levels were measured. Estimation of serum aspartate transaminases (AST), ALTs, and alkaline phosphatase (ALP) was assayed by standard photometric methods in autoanalyzer ERBA-XL (EM-200) using commercially available kits. OPN was estimated by using commercial kit based on enzyme-linked immunosorbent assay. Results The parameters of the liver function tests such as AST, ALT, and ALP were significantly increased in patients with ALD (p < 0.001) when compared with the healthy control subjects. In the present study, significantly increased levels of γ-glutamyl transferases and OPN were found in patients with ALD (p < 0.001) when compared with the control subjects. OPN showed significant positive correlations with AST (r = 0.76, p < 0.001), ALT (r = 0.64, p < 0.001), ALP (r = 0.68, p < 0.001), and GGT (r = 0.61, p < 0.001). Conclusion The present study focuses on the role of GGT and OPN that are sensitive indicators of liver cell injury and are most helpful in recognizing hepatocellular diseases such as ALD, hepatitis, and liver cirrhosis. Hence, the pattern of the GGT and OPN levels elevation can be helpful diagnostically.

scholarly journals Role of Serum Osteopontin as a Biomarker in the Diagnosis of Alcoholic Liver Disease

2020 ◽  
Author(s):  
Sanjiv Kumar Bansal ◽  
Arpita Suri ◽  
Naveen Kumar Singh
Keyword(s):  
Liver Disease ◽  
Alcohol Consumption ◽  
Alcoholic Liver Disease ◽  
Matrix Protein ◽  
Liver Function Tests ◽  
Extracellular Matrix Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gamma Glutamyl Transferase ◽  
Roc Curve Analysis ◽  
Glutamyl Transferase

Introduction: Alcohol consumption is a major cause of liver disease worldwide. The amount of alcohol ingested is the most important risk factor for the development of Alcoholic Liver Disease (ALD). Osteopontin (OPN) is an extracellular matrix protein that is markedly up-regulated in patients with ALD. Aim: To determine the best predictor of ALD among Serum Aspartate Transaminase (AST)/Alanine Transaminase (ALT) ratio, Alkaline Phosphatase (ALP), Gamma Glutamyl Transferase (GGT) and serum OPN. Materials and Methods: Clinically diagnosed cases of ALD (N=60) with alcohol consumption ≥100 gm/day, for more than eight years and age and gender matched healthy controls (N=60) were recruited for the study. Estimation of AST, ALT, ALP and GGT were assayed by standard photometric methods in auto analyser ERBA-XL (EM-200) and plasma OPN was estimated by using commercial kit based on Enzyme-Linked Immunosorbent Assay (ELISA). Receiver Operator Characteristic (ROC) curve analysis was done to establish the best predictor of ALD among the markers. Results: The parameters of the liver function tests such as AST, ALT, ALP were significantly increased in the cases (p<0.001) as compared to controls. In the study, there was a significant increase in the level of OPN and GGT in the patients with ALD (p<0.001) as compared to controls. OPN showed significant positive correlations with AST (r=0.76, p<0.001), ALT (r=0.64, p<0.001), ALP (r=0.68, p<0.001). Upon ROC analysis, OPN had the maximum area (0.998) under curve as compared to GGT and AST/ALT ratio. Conclusion: OPN is a better predictor of ALD as compared to GGT and AST/ALT ratio.

scholarly journals Újabb adatok a nem alkoholos zsírmáj patogeneziséhez

2017 ◽  
Vol 158 (23) ◽  
pp. 882-894
Author(s):  
Alajos Pár ◽  
Gabriella Pár
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Cell Injury ◽  
Bacterial Overgrowth ◽  
Small Intestinal Bacterial Overgrowth ◽  
Alcoholic Fatty Liver ◽  
Liver Cell Injury ◽  
Genetic And Environmental Factors ◽  
Alcoholic Fatty Liver Disease

Abstract: Non alcoholic fatty liver disease is the hepatic manifestation of metabolic syndrome, and the most common liver disease. Its more aggressive form is the non alcoholic steatohepatitis. Multiple genetic and environmental factors lead to the accumulation of triglicerides and the inflammatory cascade. High fat diet, obesity, adipocyte dysfunction with cytokine production, insulin resistance and increased lipolysis with free fatty acid flux into the liver – all are the drivers of liver cell injury. Activation of inflammasome by damage- or pathogen-associated molecular patterns results in “steril inflammation” and immune response, while the hepatic stellate cells and progenitor cells lead to fibrogenesis. Small intestinal bacterial overgrowth and gut dysbiosis are also of pivotal importance in the inflammation. Among the susceptible genetic factors, mutations of patatin-like phospholipase domain containing 3 and the transmembrane 6 superfamily 2 genes play a role in the development and progression of the disease, similarly as do epigenetic regulators such as microRNAs and extracellular vesicles. Better understanding of the pathogenesis of non alcoholic fatty liver disease may identify novel therapeutic agents that improve the outcome of the disease. Orv Hetil. 2017; 158(23): 882–894.

Studies of human zinc kinetics using the stable isotope 70Zn

1993 ◽  
Vol 84 (1) ◽  
pp. 113-117 ◽  
Author(s):  
N. M. Lowe ◽  
A. Green ◽  
J. M. Rhodes ◽  
M. Lombard ◽  
R. Jalan ◽  
...  
Keyword(s):  
Liver Disease ◽  
Alcoholic Liver Disease ◽  
Turnover Rate ◽  
Kinetic Studies ◽  
Normal Subjects ◽  
Short Term ◽  
Control Subjects ◽  
Two Component ◽  
Kinetics Of ◽  
Fractional Turnover

1. The short-term (120 min) kinetics of Zn turnover has been studied in control subjects and patients with alcoholic liver disease after intravenous injection of 0.5 mg of 96.5% enriched 70ZnCl2. 2. The 70Zn enrichment of plasma was found closely to obey two-compartment kinetics and the derived two-component decay equation has been used to calculate the size and turnover of the initial two rapidly exchanging pools of body Zn. 3. In normal subjects isotopic Zn appears initially to equilibrate with the whole of the plasma Zn which comprises the first metabolic compartment, pool a. This has a size of 0.72 ± 0.1 μmol/kg. 70Zn equilibration then occurs with a second compartment, pool b, consistent with a rapidly exchanging liver Zn pool of size 3.60 ± 0.93 μmol/kg. The fractional turnover rate of pool b was found to be fivefold slower than that of pool a. 4. In the alcoholic group an expansion of pool a was observed (1.63 ± 0.39 μmol/kg), but the size of the second pool was not significantly different from that of control subjects (5.55 ± 1.0 μmol/kg), although its fractional turnover was significantly increased (Kab: control subjects, 0.018 ± 0.002 min−1, alcoholic patients, 0.031 ± 0.006 min−1). 5. These data therefore demonstrate that kinetic studies using stable isotopes of Zn can provide novel information on exchangeable Zn pools in man, but provide no support for the possibility of an underlying Zn depletion in patients with alcoholic liver disease.

P292 ROLE OF NECROPTOSIS IN PATIENTS WITH LIVER DISEASE AND IN RAT LIVER CELL INJURY

2014 ◽  
Vol 60 (1) ◽  
pp. S162-S163
Author(s):  
M.B. Afonso ◽  
D.M.S. Ferreira ◽  
H. Cortez-Pinto ◽  
R.E. Castro ◽  
C.M.P. Rodrigues
Keyword(s):  
Liver Disease ◽  
Rat Liver ◽  
Liver Cell ◽  
Cell Injury ◽  
Liver Cell Injury

scholarly journals Evaluation of Protective Activity of Curcumin in Reducing Methotrexate Induced Liver Cells Injury: An Experimental Study on Iraqi White Domestic Rabbits

2018 ◽  
Vol 26 (5) ◽  
pp. 145-157
Author(s):  
Hussain Abady Aljebori ◽  
Ali H Abady ◽  
Isra’a Mahdi Al-Sudani
Keyword(s):  
Liver Cell ◽  
Cell Injury ◽  
Antioxidant Properties ◽  
Concomitant Administration ◽  
Liver Function Tests ◽  
Food Supplement ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Liver Cell Injury ◽  
Domestic Rabbits

Background: Hepatotoxicity is a common problem in medical practice, most of the commonly used drugs are potentially hepatotoxic. Although Methotrexate is a hepa- toxic drug, it is widely used in the treatment of many cancerous and non-cancerous conditions because of its cytotoxic and immunosuppressant activity. Curcumin con- tains a variety of natural substances with antioxidant properties, it is widely used in  folk medicine.Antioxidant activity of Curcumin can reduce liver cell injury induced by Methotrexate administration. Objective: The research aims to study the methotrexate hepatoxicity on rabbits, and the hepatoprotective activity of Curcumin. Materials and Methods: Thirty white domestic rabbits were bought from animal market and grouped randomly into three groups; control group received intraperitoneal normal saline, methotrexate group received 6.5 mg/Kgm body weight intraperitoneal methotrexate, and curcumin group received oral Curcumin in addition to intraperitoneal methotrexate. Results: The study showed abnormal liver function tests, INR, liver tissues oxida- tive markers, and liver cell injury on histopathology in Methotrexate group, and normal findings in Curcumin groups. Conclusion: It is concluded that the Methotrexate is a hepatotoxic drug. The results also shoe that the concomitant administration of Curcumin reduced hepatotoxicity. Recommendation: It is recommended to use of Curcumin in clinical practice as a food supplement to patient receiving methotrexate to reduce hepatotoxicity.

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