The Comparison of Hemoglobin Values Measured by Blood and Continuous Non-Invasive Monitoring (SpHb) in Newborn Infants

2020 ◽  
Author(s):  
Halil Kazanasmaz ◽  
Mahmut Demir
Keyword(s):  
Neonatal Intensive Care ◽  
Venous Blood ◽  
Newborn Infants ◽  
Blood Sampling ◽  
Neonatal Intensive Care Units ◽  
P Value ◽  
Invasive Monitoring ◽  
Non Invasive ◽  
The Difference ◽  
Venous Blood Sampling

Abstract Objective Hemoglobin (Hb) measurement is one of the most commonly used laboratory tests in medical practice. Unnecessary blood sampling, especially in neonatal intensive care units (NICUs), contributes to iatrogenic anemia. Continuous non-invasive monitoring of total Hb (SpHb) was compared with invasive venous blood samples (tHb) in NICU patients. Methods Three hundred and ten patients were identified in NICU. Non-invasive Hb measurement was performed immediately before venous blood sampling and comparison of invasive with non-invasive values was undertaken. Results There was a strongly positive correlation between SpHb and tHb (r = 0.965, p < 0.001). Bland–Altman analysis was performed in 95% limits of agreement for Hb values measured by both methods. The mean bias between tHb and SpHb measurements was 0.05 g/dl (−1.85 to 1.96). In Passing–Bablok regression analysis, the CUSUM test p value was found to be 0.98 for Hb levels measured by SpHb and tHb; and the difference between the methods was not significant. Conclusion In newborns, SpHb method offers reliable Hb values, which are comparable with the more traditional tHb method. Continuous non-invasive monitoring of total Hb may help prevent unnecessary blood sampling and iatrogenic anemia. Further clinical studies are required for the effectiveness of the method in critically ill patients with circulatory disorders.

scholarly journals THE REDUCTION OF ANXIETY AND PAIN DURING VENOUS BLOOD SAMPLING USING HYPNO-EFT (EMOTIONAL FREEDOM TECHNIQUES) METHOD

2018 ◽  
Vol 4 (2) ◽  
pp. 67-72
Author(s):  
Diah Navianti ◽  
Ardiya Garini ◽  
Karneli Karneli
Keyword(s):  
Rating Scale ◽  
Venous Blood ◽  
Blood Sampling ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Minimum Value ◽  
Average Pain ◽  
Severe Anxiety ◽  
Venous Blood Sampling

Background: Venous blood sampling has several risks of complication, the basic cause was emotional factor, such as anxiety and pain, which remain important to be solved. Objective: The aim of this study was to determine the effect of hypno–EFT to reduce the anxiety and pain during venous blood sampling. Methods: The design of the study was quasi experimental, with pretest posttest with control group design. The sampling technique was simple random sampling to select 52 respondents. Anxiety was measured using a Hamilton anxiety rating scale (HARS) modification and pain was measured using the Visual analogue scale (VAS) method. Wilcoxon and Manova multivariate test were used for data analysis.Results: Before hypno-EFT there was 1 person (2.9%) did not experience anxiety, 9 people (25.7%) with mild anxiety, 20 people (57.1%) with moderate anxiety and 5 people (14.3%) with severe anxiety. After hypno-EFT therapy, there were 23 people (65.7%) did not get anxiety, mild anxiety were 11 people (31.4%) and severe anxiety was found in 1 person (2.9%). For the average pain of  respondent in venipuncture before hypno-EFT was 3.20 with a median 3.00 and a standard deviation was 1.91. The minimum value of pain was 0 and 9 in maximum. After therapy Hypno-EFT, the average pain was 1.54  and the minimum value of pain was 0 and 6 at maximum. Statistical  result  showed that the p-value of anxiety and pain was 0.000 (0.05)Conclusion: There was a significant effect of hypno-EFT therapy to decrease the anxiety and pain during venous blood sampling. This method is recommended as an alternative procedure in venous blood sampling with complicating factors.

scholarly journals Improving the Quality of Venous Blood Sampling Procedure (Phlebotomy): Avoiding Tourniquet Use

2021 ◽  
Author(s):  
Francisco Freitas ◽  
Mónica Alves
Keyword(s):  
Venous Blood ◽  
Sampling Procedure ◽  
T Test ◽  
Blood Sampling ◽  
Age Group ◽  
Daily Data ◽  
Significant Difference ◽  
The Difference ◽  
Without Tourniquet ◽  
Venous Blood Sampling

Abstract Background Phlebotomy guidelines discourage tourniquet use whenever possible. We assessed phlebotomists' capability of not using the tourniquet in venous blood sampling, hypothesizing it to be equal to 50% of the patients attended, and identifying the most frequent venipuncture site. Materials and Methods We assigned two phlebotomists of the same age (41 years) and experience (20 years) to record 10 phlebotomy days, the first with prioritized and the latter with nonprioritized patients. Each acquired daily data for the number of attended patients, age, gender, frequency of nontourniquet usage, and punctured vein. To test our work hypothesis we used the two-tailed single sample t-test. Differences between age-group means and nontourniquet use means by each phlebotomist were tested by two-tailed t-test for independent means. Results In 10 phlebotomy days, 683 patients were attended (males 43.2%). We found no statistically significant difference between age-group means. The combined capability of nontourniquet use was 50.5%, which did not differ from our null hypothesis, but the difference in individual group means was statistically significant, the means being 33% and 66.9% (prioritized vs. nonprioritized). The medial cubital vein was the most prone to be punctured (77.7%). Conclusion Performing phlebotomies without tourniquet was possible in at least half of the attended patients, though it was more limited in specific group populations.

Perbedaan Penggunaan Antikoagulan K2EDTA DAN K3EDTA Terhadap Hasil Pemeriksaan Indeks Eritrosit

2018 ◽  
Vol 1 (2) ◽  
pp. 114
Author(s):  
Wahdaniah Wahdaniah ◽  
Sri Tumpuk
Keyword(s):  
Screening Test ◽  
Venous Blood ◽  
The Body ◽  
Ethylene Diamine ◽  
P Value ◽  
Cross Sectional ◽  
Significant Difference ◽  
Ethylene Diamine Tetra Acetate ◽  
The Difference ◽  
Index Value

Abstract: Routine blood examination is the earliest blood test or screening test to determine the diagnosis of an abnormality. Blood easily froze if it is outside the body and can be prevented by the addition of anticoagulants, one of which Ethylene Diamine Tetra Acetate (EDTA). Currently available vacuum tubes containing EDTA anticoagulants in the form of K2EDTA and K3EDTA. K3EDTA is usually a salt that has better stability than other EDTA salts because it shows a pH approaching a blood pH of about 6.4. The purpose of this research is to know the difference of erythrocyte index results include MCH, MCV and MCHC using K3EDTA anticoagulant with K2EDTA. This research is a cross sectional design. This study used venous blood samples mixed with K2EDTA anticoagulant and venous blood mixed with K3EDTA anticoagulants, each of 30 samples. Data were collected and analyzed using paired different test. Based on data analysis that has been done on MCH examination, p value <0,05 then there is a significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value. Then on the examination of MCV and MCHC obtained p value <0.05 then there is no significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value.Abstrak: Pemeriksaan darah rutin merupakan pemeriksaan darah yang paling awal atau screening test untuk mengetahui diagnosis suatu kelainan. Darah mudah membeku jika berada diluar tubuh dan bisa dicegah dengan penambahan antikoagulan, salah satunya Ethylene Diamine Tetra Acetate (EDTA). Dewasa ini telah tersedia tabung vakum yang sudah berisi antikoagulan EDTA dalam bentuk  K2EDTA dan  K3EDTA. K3EDTA  biasanya berupa garam yang mempunyai stabilitas yang lebih baik dari garam EDTA yang lain karena menunjukkan pH yang mendekati pH darah yaitu sekitar 6,4. Tujuan dari penelitian ini adalah untuk mengetahui perbedaan hasil indeks eritrosit meliputi MCH, MCV dan MCHC menggunakan antikoagulan K3EDTA dengan K2EDTA. Penelitian ini merupakan penelitian dengan desain cross sectional. Penelitian ini menggunakan sampel darah vena yang dicampur dengan antikoagulan K2EDTA dan darah vena yang dicampur dengan antikoagulan K3EDTA, masing-masing sebanyak 30 sampel. Data dikumpulkan dan dianalisis menggunakan uji beda berpasangan. Berdasarkan analisis data yang telah dilakukan pada pemeriksaan MCH didapatkan nilai p < 0,05 maka ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit. Kemudian pada pemeriksaan MCV dan MCHC didapatkan nilai p < 0,05 maka tidak ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit.

scholarly journals An Anti-Nucleocapsid Antigen Sars-Cov-2 Total Antibody Assay Finds Comparable Results in Edta-Anticoagulated Whole Blood Obtained from Capillary and Venous Blood Sampling

Data ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 105
Author(s):  
Martin Risch ◽  
Marc Kovac ◽  
Corina Risch ◽  
Dorothea Hillmann ◽  
Michael Ritzler ◽  
...  
Keyword(s):  
Whole Blood ◽  
Venous Blood ◽  
Capillary Blood ◽  
Blood Sampling ◽  
Attractive Alternative ◽  
Antibody Assay ◽  
Antibody Assays ◽  
Antibody Titers ◽  
Antibody Levels ◽  
Venous Blood Sampling

Although SARS-CoV-2 antibody assays have been found to provide valid results in EDTA-anticoagulated whole blood, so far, they have not demonstrated that antibody levels in whole blood originating from capillary blood samples are comparable to antibody levels measured in blood from a venous origin. Here, blood is drawn simultaneously by capillary and venous blood sampling. Antibody titers are determined by an assay employing electrochemiluminescence (ECLIA) and SARS-CoV-2 total immunoglobulins are detected with specificity directed against the nucleocapsid antigen. Six individuals with confirmed COVID-19 and six individuals without COVID-19 are analyzed. Antibody titers in capillary venous whole blood did not show significant differences, and when corrected for hematocrit, they did not differ from the results obtained from serum. In conclusion, capillary sampled EDTA-anticoagulated whole blood seems to be an attractive alternative matrix for the evaluation of SARS-CoV-2 antibodies when employing ECLIA for detecting total antibodies directed against nucleocapsid antibodies.

scholarly journals Improving the quality of venous blood sampling procedure (phlebotomy): avoiding tourniquet use

2020 ◽  
Author(s):  
Francisco Freitas ◽  
Mónica Alves
Keyword(s):  
Venous Blood ◽  
Real Life ◽  
Sampling Procedure ◽  
T Test ◽  
Blood Sampling ◽  
Age Group ◽  
The Individual ◽  
Sampling Procedures ◽  
Without Tourniquet ◽  
Venous Blood Sampling

AbstractBackgroundGuidelines for venous blood sampling procedure (phlebotomy) discourage tourniquet use whenever possible. Here, we aimed to assess the Biomedical Scientists capability of not using the tourniquet in phlebotomy, which we hypothesized to be equal to 50% of the patients attended, and identifying the most frequent venipuncture site.Materials and MethodsWe selected and assigned two (BMS) with the same age (41 years) and experience (20 years) to record ten phlebotomy days, the first with prioritized and the latter with non-prioritized patients. In a simple record form, each acquired daily data for the number of attended patients, age and gender, the frequency of non-tourniquet usage and the punctured vein. To test our work hypothesis we used the two-tailed single sample t-test (p < 0.05). Differences between age-group means and non-tourniquet use means by each BMS were tested by two-tailed t-test for independent means (p < 0.05).ResultsIn 10 phlebotomy days 683 patients were attended, with males representing 43,2% of the population. We found no statistically difference between age-group means. The combined capability of non-tourniquet use was 50,5%, which did not differ from our null hypothesis, but the individual group-means were statistically different, being 33% and 66.9% in the prioritized vs non-prioritized group. The medial cubital vein was the most prone to be punctured (77,7%).ConclusionsWe have shown that performing phlebotomies without tourniquet use is possible and desirable in at least half of the attended patients, though being more limited in specific group populations. Our results provide room for quality improvement in the laboratory pre-analytical phase.Key points summaryWe assessed the capability of Biomedical Scientists not using the tourniquet in real life blood sampling procedures for diagnostic purposes.Blood was collected from at least half of the attended patients without tourniquet use.Biomedical Scientists were able to prioritize the antecubital veins without tourniquet application (medial cubital vein the most prone to be punctured - 78% of attempts).

scholarly journals Significance of serum-pleural effusion albumin gradient in differentiating transudative and exudative pleural effusions in comparison to light’s criteria

2017 ◽  
Vol 4 (2) ◽  
pp. 457
Author(s):  
Sujatha G. ◽  
Vindhya P. ◽  
Kalyan Kumar K.
Keyword(s):  
Pleural Effusion ◽  
Clinical Outcome ◽  
Pleural Fluid ◽  
Venous Blood ◽  
P Value ◽  
Protein Ratio ◽  
Clinical Disorder ◽  
Significant Difference ◽  
The Difference ◽  
Sensitivity Specificity

Background: Approximately one million patients develop pleural effusion every year. It is a common clinical disorder and is either a manifestation or a complication of one or other respiratory or non-respiratory disorders. It leads to serious prognosis, if not diagnosed and treated properly. To calculate SEAG and Light’s criteria and to compare SEAG with Light’s criteria in analyzing pleural effusions.Methods: A total of hundred patients were selected for the study. Pleural fluid of patients who met the inclusion and exclusion criteria were collected, when pleural fluid is being tapped for diagnostic thoracocentesis. Venous blood sample was collected along with diagnostic thoracocentesis or within 24 hours of thoracocentesis.  Written informed consent was obtained from them for thoracocentesis.Results: In our study we compared the clinical outcome with outcome as per Pleural fluid/Serum protein ratio (p value of <0.0001), pleural fluid/serum LDH (p value of <0.0001) and pleural fluid LDH (p value of <0.0001) separately and the p values were statistically significant. The sensitivity, specificity, PPV and NPV of Light’s criteria were 77.2%, 100%, 100%, 93.9% respectively. We compared Light’s criteria outcome with clinical outcome and the difference was statistically significant (p value of <0.0001). SEAG showed 100% sensitivity, 97.43% specificity, 91.6% PPV and is 91.66% and NPV is 100%. We compared the clinical outcome with SEAG and there was statistically significant difference (p value of <0.0001). We compared SEAG with Light’s criteria and the difference was statistically significant (p <0.0001). We compared Light’s plus pleural fluid protein gradient with SEAG and the difference is statistically significant (p value of <0.0001).Conclusions: SEAG is more sensitive for classifying transudates and more specific for exudates than Light’s criteria.

scholarly journals How to Perform Umbilical Cord Arterial and Venous Blood Sampling in Neonatal Foals

2019 ◽  
Vol 73 ◽  
pp. 24-29 ◽  
Author(s):  
Sunita S. Jeawon ◽  
Lisa M. Katz ◽  
Noreen P. Galvin ◽  
Stephen D. Cahalan ◽  
Vivienne E. Duggan
Keyword(s):  
Umbilical Cord ◽  
Venous Blood ◽  
Blood Sampling ◽  
Venous Blood Sampling
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