Phenolic Constituents in Dried Flowers of Aloe vera (Aloe barbadensis) and their in vitro Antioxidative Capacity

Ethnomedicinally, the family Liliaceae is prominent in controlling skin, wound and fungal infections. A carboxylic fatty acid derivative has been isolated from Aloe vera leaves and its structure was elucidated on the basis of NMR and FT-IR analysis. The compound was formulated as ointment with the aid of polymer based delivery agent. The ointment and the uncontaminated fatty acid have been scrutinized aimed at their anti-infectious potential. Individually, the pure compound and the formulated ointment exhibited growth inhibitory activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Candida albicans and Talaromyces flavus. Minimum inhibitory concentration (MIC) values were found to be appreciably low, ranging between 0.30 and 2.50 mg/ml. The formulated ointment displayed highly significant inhibitory activities against all six pathogens compared to the unformulated compound. The MIC values of the ointment ranged between 0.08 and 1.25 mg/g. The in vitro antibiotic activity studies discovered that, both the pure compound and the formulated ointment showed potency against the selected multi-resistant microorganisms tested in the current work. These findings suggest that the isolated carboxylic acid derivative may be beneficial in the discovery of antibiotics highly potent against drug-resistant pathogens. Furthermore, it may add to the improvement of preservatives in the food industry. Lastly, it could be considered as new source of natural antibiotics in the pharmaceutical industry.

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5.Evaluación de dos medios de cultivo: aloe vera (aloe barbadensis miller) y la pulpa de papel como gelificantes alternativos del agar-agar para la propagación in-vitro de la variedad de papa andinita (SOLANUM TUBEROSUM L)

Revista EDUCARE - UPEL-IPB - Segunda Nueva Etapa 2.0 ◽

10.46498/reduipb.v21i2.53 ◽

2018 ◽

Vol 21 (2) ◽

pp. 58-67 ◽

Cited By ~ 1

Author(s):

Juan Marcos Oviedo Montero (Colegio Pablo VI) ◽

Albany García Montero

Keyword(s):

Solanum Tuberosum ◽

Solanum Tuberosum L ◽

Aloe Vera ◽

Aloe Barbadensis

El estudio tiene como propósito evaluar el efecto de la aplicación de Aloe Vera (Aloe Barbadensis Miller) y la Pulpa de Papel seca como gelificantes alternativos del agar-agar para la preparación de medios de cultivo para la propagación in-vitro de la variedad de papa Andinita (SolanumTuberosum L.). El mismo es realizado bajo el enfoque cuantitativo y paradigma positivista, adoptando un estudio experimental carácter explicativo. El procedimiento se realizó utilizando como base la solución Murashige-Skooge (MS) sustituyendo el agar-agar y aplicando técnicas tradicionales de cultivo in-vitro. La población objeto de estudio estuvo conformada por 300 vitro-plantas de un lote de papa Andinita (SolanumTuberosum L.) pertenecientes al (INIA) de las cuales se tomó como muestra 100 vitro-plantas de dicho lote cultivo. Para el tratamiento se seleccionó un testigo de la vitro-planta el cual tuvo un medio tradicional, mientras que 4 grupos fueron sometidos a diferentes concentraciones de los medios sustitutos para el Agar-Agar. Se espera que esta investigación permita generar medios alternos que contribuyan a la reducción de la utilización del Agar en los medios de cultivos in vitro.

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Enhanced in vitro caulogenesis and quantitative estimation of aloin through HPLC in Aloe vera

Israel Journal of Plant Sciences ◽

10.1080/07929978.2014.961388 ◽

2013 ◽

Vol 61 (1-4) ◽

pp. 57-63 ◽

Cited By ~ 2

Author(s):

V. Sharma ◽

M. Guleria ◽

P. Chaudhary

Keyword(s):

Large Scale ◽

Quantitative Estimation ◽

Aloe Vera ◽

Shoot Proliferation ◽

Multiple Shoots ◽

Fold Increase ◽

Nutrient Media ◽

Aloe Barbadensis ◽

Shoot Bud

Aloe vera(Liliaceae) is well known for its medicinal properties. It contains flavonoids, saponins, tannins, alkaloids and other compounds which exhibit medicinal, cosmetic and pharmacological properties. There is a lack of production of aloe leaf to meet the industry demand and therefore the means to facilitate large-scale aloe cultivation schemes need to be developed. Because sexual reproduction by seeds is almost ineffective inAloe veradue to male sterility and vegetative propagation by offshoots is only possible during the growing seasons, there is a need to develop a propagation method to facilitate large-scale cultivation.In vitroregeneration of medicinal plants is important and there is the potential for the production of high-quality plant-based medicine. In the present study, an attempt has been made to enhancein vitrocaulogenesis and aloin content in plants in the presence of a precursor (tryptophan) in the nutrient media. Forin vitroculture establishment and shoot bud multiplication, MS basal media were used supplemented with different concentrations and different combinations of growth regulators such as BAP (6-benzylaminopurine) and IAA (3-indole acetic acid). Shoot proliferation was optimal in MS medium containing 2.0 mg l−1BAP. For evaluation ofin vitroenhancement of aloin production the precursor tryptophan was added to the nutrient media at different levels (5, 10, 15 and 20 mg l−1). Addition of 20 mg l−1tryptophan induced a 2.43-fold increase in aloin content in multiple shoots cultures ofAloe barbadensis.

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In Vitro Susceptibilities of Shigella flexneri and Streptococcus pyogenes to Inner Gel of Aloe barbadensis Miller

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.47.3.1137-1139.2003 ◽

2003 ◽

Vol 47 (3) ◽

pp. 1137-1139 ◽

Cited By ~ 37

Author(s):

Valerie A. Ferro ◽

Fiona Bradbury ◽

Pamela Cameron ◽

Eisin Shakir ◽

Sabita R. Rahman ◽

...

Keyword(s):

Streptococcus Pyogenes ◽

Shigella Flexneri ◽

Aloe Vera ◽

Aloe Barbadensis ◽

Whole Plant ◽

Health Products

ABSTRACT Aloe barbadensis Miller (or Aloe vera) has widespread use in health products, and despite numerous reports on the whole plant, little work has been performed on the inner gel, which has been used extensively in these products. This report describes the in vitro susceptibilities of two bacteria to this component.

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DAYA HAMBAT EKSTRAK LIDAH BUAYA (Aloe barbadensis Miller) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus SECARA IN VITRO

JURNAL WIDYA BIOLOGI ◽

10.32795/widyabiologi.v10i01.234 ◽

2019 ◽

Vol 10 (01) ◽

pp. 23-32

Author(s):

Pramesti Indah Prabasari ◽

I M Sumarya ◽

N.K.A. Juliasih

Keyword(s):

Staphylococcus Aureus ◽

Aloe Vera ◽

Inhibition Zone ◽

Inhibitory Effect ◽

Positive Control ◽

Control Group ◽

Aloe Barbadensis ◽

Control Group Design ◽

Inhibitory Power

Aloe vera (Aloe barbadensis Miller) contains anthraquinone compounds, flavonoids and saponins which are thought to have antibacterial effects so as to inhibit bacterial growth. The purpose of the study was to determine the inhibitory power of Aloe barbadensis Miller on the growth of Staphylococcus aureus bacteria. The pure experimental study was conducted with The Randomized Posttest Only Control Group Design using six (6) treatments, namely aloe vera extract with a concentration of 25%, 50%, 75%, 100%, Chloramphenicol 30 mcg antibiotics as a positive control, and sterile aquadest as a control negative for Staphylococcus aureus bacteria grown on Mueller Hinton media. After being treated, it was incubated for 24 hours and measured its inhibitory power. The growth inhibition data of Staphylococcus aureus bacteria in the form of inhibition zone diameter was analyzed statistically by Kruskal Wallis test, at the level of confidence α = 0.05. The results showed that the average inhibitory effect of Aloe vera extract on the growth of Staphylococcus aureus bacteria in concentrations of 25%, 50%, 75% and 100% were 0 ± 0 mm; 7.125 ± 0.1250 mm; 8.375 ± 0.23936 mm; and 9.0 ± 0.40825 mm. Based on the results of the study concluded that aloe vera extract can inhibit the growth of Staphylococcus aureus bacteria. The higher the concentration of Aloe vera extract the greater the inhibitory effect on the growth of Staphylococcus aureus bacteria.

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In vitro and in vivo antioxidant activities of polysaccharide purified from aloe vera (Aloe barbadensis) gel

Carbohydrate Polymers ◽

10.1016/j.carbpol.2013.07.091 ◽

2014 ◽

Vol 99 ◽

pp. 365-371 ◽

Cited By ~ 79

Author(s):

Min-Cheol Kang ◽

Seo Young Kim ◽

Yoon Taek Kim ◽

Eun-A. Kim ◽

Seung-Hong Lee ◽

...

Keyword(s):

Antioxidant Activities ◽

Aloe Vera ◽

Aloe Barbadensis

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In vitro evaluation of anthraquinones from Aloe vera (Aloe barbadensis Miller) roots and several derivatives against strains of influenza virus

Industrial Crops and Products ◽

10.1016/j.indcrop.2019.02.056 ◽

2019 ◽

Vol 132 ◽

pp. 468-475 ◽

Cited By ~ 9

Author(s):

Rocío Borges-Argáez ◽

Reyna Chan-Balan ◽

Lisseth Cetina-Montejo ◽

Guadalupe Ayora-Talavera ◽

Pablo Sansores-Peraza ◽

...

Keyword(s):

Influenza Virus ◽

Aloe Vera ◽

Aloe Barbadensis ◽

In Vitro Evaluation

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Preliminary Phytochemical Analysis: In-Vitro Comparative Evaluation of Anti-arthritic and Anti-inflammatory Potential of Some Traditionally Used Medicinal Plants

Dose-Response ◽

10.1177/15593258211069720 ◽

2022 ◽

Vol 20 (1) ◽

pp. 155932582110697

Author(s):

Sundas Farooq ◽

Ghazala Shaheen ◽

Hafiz Muhammad Asif ◽

Muhammad Rahil Aslam ◽

Rabia Zahid ◽

...

Keyword(s):

Medicinal Plants ◽

Protein Denaturation ◽

Aloe Vera ◽

Stabilization Method ◽

Membrane Stabilization ◽

Aloe Barbadensis ◽

Standard Drug ◽

Colchicum Autumnale ◽

Anti Inflammatory

Background: Colchicum autumnale, Strychnous nux-vomica and Aloe barbadensis are the medicinal plants clinically utilized for the management of rhuematic disorders. Purpose: The present work was focused to evaluate the in-vitro anti-arthritic and anti-inflammatory activities of Colchicum ( Colchicum autumnale), Nux-vomica ( Strychnous nux-vomica), and Aloe-vera ( Aloe barbadensis). Research Design: Primarily, the aqueous-ethanolic extracts of these medicinal plants were phytochemically screened followed by Fourier Transform Infrared (FTIR) analysis. Anti-arthritic activity by protein denaturation method and anti-inflammatory activity by human red blood cell (HRBC) membrane stabilization method at the concentration of 125, 250, and 500 µg/mL along with standard were performed. Results: Phytochemical screening revealed that alkaloids, saponins, terpenoids, phenols, and anthraquinones were found in all the extracts, and organic acids, amine group, aromatic or aliphatic compounds, esters and halogens, and phenolics were identified by FTIR. Protein denaturation method revealed that colchicum, nux-vomica, and aloe-vera showed maximum 98.5%, 99.6%, and 72.3% of inhibition at 500 µg/mL compared with that of standard drug, that is, Diclofenac sodium. Membrane stabilization method showed that colchicum, nux-vomica, and aloe-vera showed maximum 40.20%, 35.67%, and 40.1% protection at 500 µg/mL when compared with standard drug. Conclusion: It is concluded from the current study that extracts of colchicum, nux-vomica, and aloe-vera showed more potent effect and thus can be used as alternative options for the management of inflammatory and arthritic ailments.

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