scholarly journals In Vitro Susceptibilities of Shigella flexneri and Streptococcus pyogenes to Inner Gel of Aloe barbadensis Miller

2003 ◽  
Vol 47 (3) ◽  
pp. 1137-1139 ◽  
Author(s):  
Valerie A. Ferro ◽  
Fiona Bradbury ◽  
Pamela Cameron ◽  
Eisin Shakir ◽  
Sabita R. Rahman ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Shigella Flexneri ◽  
Aloe Vera ◽  
Aloe Barbadensis ◽  
Whole Plant ◽  
Health Products

ABSTRACT Aloe barbadensis Miller (or Aloe vera) has widespread use in health products, and despite numerous reports on the whole plant, little work has been performed on the inner gel, which has been used extensively in these products. This report describes the in vitro susceptibilities of two bacteria to this component.

scholarly journals Effectiveness of isolated compound from Aloe barbadensis Miller and its formulated ointment against bacteria and fungi

2021 ◽  
Vol 8 (2) ◽  
pp. 47-53
Author(s):  
Martin Ntiamoah Donkor ◽  
Ngmenpone Kuubabongnaa ◽  
Addai-Mensah Donkor
Keyword(s):  
Fatty Acid ◽  
Acid Derivative ◽  
Fungal Infections ◽  
Fatty Acid Derivative ◽  
Aloe Vera ◽  
Skin Wound ◽  
Pure Compound ◽  
Aloe Barbadensis ◽  
Talaromyces Flavus

Ethnomedicinally, the family Liliaceae is prominent in controlling skin, wound and fungal infections. A carboxylic fatty acid derivative has been isolated from Aloe vera leaves and its structure was elucidated on the basis of NMR and FT-IR analysis. The compound was formulated as ointment with the aid of polymer based delivery agent. The ointment and the uncontaminated fatty acid have been scrutinized aimed at their anti-infectious potential. Individually, the pure compound and the formulated ointment exhibited growth inhibitory activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Candida albicans and Talaromyces flavus. Minimum inhibitory concentration (MIC) values were found to be appreciably low, ranging between 0.30 and 2.50 mg/ml. The formulated ointment displayed highly significant inhibitory activities against all six pathogens compared to the unformulated compound. The MIC values of the ointment ranged between 0.08 and 1.25 mg/g. The in vitro antibiotic activity studies discovered that, both the pure compound and the formulated ointment showed potency against the selected multi-resistant microorganisms tested in the current work. These findings suggest that the isolated carboxylic acid derivative may be beneficial in the discovery of antibiotics highly potent against drug-resistant pathogens. Furthermore, it may add to the improvement of preservatives in the food industry. Lastly, it could be considered as new source of natural antibiotics in the pharmaceutical industry.

scholarly journals 5.Evaluación de dos medios de cultivo: aloe vera (aloe barbadensis miller) y la pulpa de papel como gelificantes alternativos del agar-agar para la propagación in-vitro de la variedad de papa andinita (SOLANUM TUBEROSUM L)

2018 ◽  
Vol 21 (2) ◽  
pp. 58-67 ◽  
Author(s):  
Juan Marcos Oviedo Montero (Colegio Pablo VI) ◽  
Albany García Montero
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Aloe Vera ◽  
Aloe Barbadensis

El estudio tiene como propósito evaluar el efecto de la aplicación de Aloe Vera (Aloe Barbadensis Miller) y la Pulpa de Papel seca como gelificantes alternativos del agar-agar para la preparación de medios de cultivo para la propagación in-vitro de la variedad de papa Andinita (SolanumTuberosum L.). El mismo es realizado bajo el enfoque cuantitativo y paradigma positivista, adoptando un estudio experimental carácter explicativo. El procedimiento se realizó utilizando como base la solución Murashige-Skooge (MS) sustituyendo el agar-agar y aplicando técnicas tradicionales de cultivo in-vitro. La población objeto de estudio estuvo conformada por 300 vitro-plantas de un lote de papa Andinita (SolanumTuberosum L.) pertenecientes al (INIA) de las cuales se tomó como muestra 100 vitro-plantas de dicho lote cultivo. Para el tratamiento se seleccionó un testigo de la vitro-planta el cual tuvo un medio tradicional, mientras que 4 grupos fueron sometidos a diferentes concentraciones de los medios sustitutos para el Agar-Agar. Se espera que esta investigación permita generar medios alternos que contribuyan a la reducción de la utilización del Agar en los medios de cultivos in vitro.

scholarly journals Phytochemical Screening and Antimicrobial Analysis of Fadogia andersonii Robyn Plant Extrac

2021 ◽  
Vol 25 (2) ◽  
pp. 209-213
Author(s):  
J. Nzeako ◽  
G.I. Ndukwe ◽  
J.D. Habila ◽  
E.A. Oluwabukola ◽  
I. Owoicho
Keyword(s):  
Escherichia Coli ◽  
Streptococcus Pyogenes ◽  
Cardiac Glycosides ◽  
Salmonella Typhi ◽  
Inhibitory Effect ◽  
Klebsiella Pneumonia ◽  
Phytochemical Screening ◽  
Whole Plant ◽  
The Family

Medicinal plants extracts are now generally considered as effective medicines that play a major role in modern pharmacy. The plant Fadogia andersonii belonging to the Family Rubiaceae, which is used in ethno-medicine was studied. Preliminary phytochemical analyses of the whole plant revealed the presence of the following metabolites: Saponins, terpenes, steroids, flavonoids, tannins, alkaloids, cardiac glycosides and carbohydrates. Anthraquinones was found to be absent. Antimicrobial screening of the methanol plant’s extract carried out (in vitro) on Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Bacillus cereus, Klebsiella pneumonia, Streptococcus pneumoniae, Streptococcus pyogenes, Candida albican and Aspergillus flavus showed that the extract has activity on the tested microorganisms. However, it showed no inhibitory effect against Escherichia coli. The extract was found to inhibit the growth of S. aureus, B. cereus, S. pyogenes and C. albican at 25mg/ml with a corresponding MBC at 50mg/ml. S.typhi and S. pneumonia were inhibited at 50mg/ml with a corresponding MBC at 100mg/ml. It also inhibited the growth of P. aeruginosa, K. pneumonia and A. flavus at 100mg/ml with a corresponding MBC at 200mg/ml. The observed antimicrobial effects were believed to be due to the presence of active principles which were detected in the phytochemical screening. Keywords: Phytochemicals, Antimicrobials, Fadogia andersonii Robyn

Enhanced in vitro caulogenesis and quantitative estimation of aloin through HPLC in Aloe vera

2013 ◽  
Vol 61 (1-4) ◽  
pp. 57-63 ◽  
Author(s):  
V. Sharma ◽  
M. Guleria ◽  
P. Chaudhary
Keyword(s):  
Large Scale ◽  
Quantitative Estimation ◽  
Aloe Vera ◽  
Shoot Proliferation ◽  
Multiple Shoots ◽  
Fold Increase ◽  
Nutrient Media ◽  
Aloe Barbadensis ◽  
Shoot Bud

Aloe vera(Liliaceae) is well known for its medicinal properties. It contains flavonoids, saponins, tannins, alkaloids and other compounds which exhibit medicinal, cosmetic and pharmacological properties. There is a lack of production of aloe leaf to meet the industry demand and therefore the means to facilitate large-scale aloe cultivation schemes need to be developed. Because sexual reproduction by seeds is almost ineffective inAloe veradue to male sterility and vegetative propagation by offshoots is only possible during the growing seasons, there is a need to develop a propagation method to facilitate large-scale cultivation.In vitroregeneration of medicinal plants is important and there is the potential for the production of high-quality plant-based medicine. In the present study, an attempt has been made to enhancein vitrocaulogenesis and aloin content in plants in the presence of a precursor (tryptophan) in the nutrient media. Forin vitroculture establishment and shoot bud multiplication, MS basal media were used supplemented with different concentrations and different combinations of growth regulators such as BAP (6-benzylaminopurine) and IAA (3-indole acetic acid). Shoot proliferation was optimal in MS medium containing 2.0 mg l−1BAP. For evaluation ofin vitroenhancement of aloin production the precursor tryptophan was added to the nutrient media at different levels (5, 10, 15 and 20 mg l−1). Addition of 20 mg l−1tryptophan induced a 2.43-fold increase in aloin content in multiple shoots cultures ofAloe barbadensis.

scholarly journals Screening of Antimicrobial Activity of Siddha drug Kukkiladhi Choornam

2018 ◽  
Vol 10 (1) ◽  
pp. 40-42
Author(s):  
Marimuthu Revathi ◽  
Murugesan Karthikeyan ◽  
Ashok Gnanasekaran ◽  
Ranjith MS
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Streptococcus Pyogenes ◽  
Shigella Flexneri ◽  
Salmonella Typhi ◽  
Antibacterial And Antifungal Activity ◽  
Proteus Vulgaris ◽  
Solid Media ◽  
Antibacterial And Antifungal

Plan: Kukkiladhi choornam is one of the Siddha drugs, which has been indicated for its anti-microbial properties. The aim of the present study was to validate the antibacterial and antifungal activity of Kukkiladhi choornam extract against various microorganisms. Preface: The present study examined the in-vitro screening of antimicrobial activity of Siddha drug Kukkiladhi choornam. Methodology: The extracts were tested for antimicrobial activity using agar well diffusion on solid media. The microorganisms used in the present study include Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli, Salmonella typhi, Shigella flexneri, Proteus vulgaris, and Candida albicans. Outcome: The study revealed that 50µl extract of Kukkiladhichoornam had significant inhibiting activity against Shigellaflexneri (17mm), Streptococcus pyogenes (17mm) and Candida albicans (18mm).

scholarly journals DAYA HAMBAT EKSTRAK LIDAH BUAYA (Aloe barbadensis Miller) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus SECARA IN VITRO

2019 ◽  
Vol 10 (01) ◽  
pp. 23-32
Author(s):  
Pramesti Indah Prabasari ◽  
I M Sumarya ◽  
N.K.A. Juliasih
Keyword(s):  
Staphylococcus Aureus ◽  
Aloe Vera ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Control Group ◽  
Aloe Barbadensis ◽  
Control Group Design ◽  
Inhibitory Power

Aloe vera (Aloe barbadensis Miller) contains anthraquinone compounds, flavonoids and saponins which are thought to have antibacterial effects so as to inhibit bacterial growth. The purpose of the study was to determine the inhibitory power of Aloe barbadensis Miller on the growth of Staphylococcus aureus bacteria. The pure experimental study was conducted with The Randomized Posttest Only Control Group Design using six (6) treatments, namely aloe vera extract with a concentration of 25%, 50%, 75%, 100%, Chloramphenicol 30 mcg antibiotics as a positive control, and sterile aquadest as a control negative for Staphylococcus aureus bacteria grown on Mueller Hinton media. After being treated, it was incubated for 24 hours and measured its inhibitory power. The growth inhibition data of Staphylococcus aureus bacteria in the form of inhibition zone diameter was analyzed statistically by Kruskal Wallis test, at the level of confidence α = 0.05. The results showed that the average inhibitory effect of Aloe vera extract on the growth of Staphylococcus aureus bacteria in concentrations of 25%, 50%, 75% and 100% were 0 ± 0 mm; 7.125 ± 0.1250 mm; 8.375 ± 0.23936 mm; and 9.0 ± 0.40825 mm. Based on the results of the study concluded that aloe vera extract can inhibit the growth of Staphylococcus aureus bacteria. The higher the concentration of Aloe vera extract the greater the inhibitory effect on the growth of Staphylococcus aureus bacteria.

In vitro and in vivo antioxidant activities of polysaccharide purified from aloe vera (Aloe barbadensis) gel

2014 ◽  
Vol 99 ◽  
pp. 365-371 ◽  
Author(s):  
Min-Cheol Kang ◽  
Seo Young Kim ◽  
Yoon Taek Kim ◽  
Eun-A. Kim ◽  
Seung-Hong Lee ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Aloe Vera ◽  
Aloe Barbadensis
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