Antioxidant activity of extracts of Cynomorium songaricum and their neuroprotective effects against cytotoxicity induced by Aβ25–35 on SK-N-SH neuroblastoma cells

Kefir, a fermented probiotic drink was tested for its potential anti-oxidative, anti-apoptotic, and neuroprotective effects to attenuate cellular oxidative stress on human SH-SY5Y neuroblastoma cells. Here, the antioxidant potentials of the six different kefir water samples were analysed by total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH) assays, whereas the anti-apoptotic activity on hydrogen peroxide (H2O2) induced SH-SY5Y cells was examined using MTT, AO/PI double staining, and PI/Annexin V-FITC assays. The surface and internal morphological features of SH-SY5Y cells were studied using scanning and transmission electron microscopy. The results indicate that Kefir B showed the higher TPC (1.96 ± 0.54 µg GAE/µL), TFC (1.09 ± 0.02 µg CAT eq/µL), FRAP (19.68 ± 0.11 mM FRAP eq/50 µL), and DPPH (0.45 ± 0.06 mg/mL) activities compared to the other kefir samples. The MTT and PI/Annexin V-FITC assays showed that Kefir B pre-treatment at 10 mg/mL for 48 h resulted in greater cytoprotection (97.04%), and a significantly lower percentage of necrotic cells (7.79%), respectively. The Kefir B pre-treatment also resulted in greater protection to cytoplasmic and cytoskeleton inclusion, along with the conservation of the surface morphological features and the overall integrity of SH-SY5Y cells. Our findings indicate that the anti-oxidative, anti-apoptosis, and neuroprotective effects of kefir were mediated via the upregulation of SOD and catalase, as well as the modulation of apoptotic genes (Tp73, Bax, and Bcl-2).

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Genome-Wide Microarray Analysis of the Differential Neuroprotective Effects of Antioxidants in Neuroblastoma Cells Overexpressing the Familial Parkinson’s Disease α-Synuclein A53T Mutation

Neurochemical Research ◽

10.1007/s11064-009-0038-1 ◽

2009 ◽

Vol 35 (1) ◽

pp. 130-142 ◽

Cited By ~ 15

Author(s):

Lei Ma ◽

Tracy T. Cao ◽

Geeta Kandpal ◽

Lee Warren ◽

J. Fred Hess ◽

...

Keyword(s):

Parkinson’S Disease ◽

Parkinson's Disease ◽

Microarray Analysis ◽

Neuroblastoma Cells ◽

Neuroprotective Effects ◽

Genome Wide ◽

Familial Parkinson’S Disease ◽

A53t Mutation

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The antioxidant xanthorrhizol prevents amyloid-β-induced oxidative modification and inactivation of neprilysin

Bioscience Reports ◽

10.1042/bsr20171611 ◽

2018 ◽

Vol 38 (1) ◽

Cited By ~ 6

Author(s):

Chol Seung Lim ◽

Jung-Soo Han

Keyword(s):

Therapeutic Potential ◽

Neuroblastoma Cells ◽

Amyloid Β ◽

Enzymatic Activities ◽

Oxidative Modification ◽

Amyloid Β Peptide ◽

Oxygen Species ◽

Human Neuroblastoma Cells ◽

Neuroprotective Effects ◽

Human Neuroblastoma

Activity of neprilysin (NEP), the major protease which cleaves amyloid-β peptide (Aβ), is reportedly reduced in the brains of patients with Alzheimer’s disease (AD). Accumulation of Aβ generates reactive oxygen species (ROS) such as 4-hydroxynonenal (HNE), and then reduces activities of Aβ-degrading enzymes including NEP. Xanthorrhizol (Xan), a natural sesquiterpenoid, has been reported to possess antioxidant and anti-inflammatory properties. The present study examined the effects of Xan on HNE- or oligomeric Aβ42-induced oxidative modification of NEP protein. Xan was added to the HNE- or oligomeric Aβ42-treated SK-N-SH human neuroblastoma cells and then levels, oxidative modification and enzymatic activities of NEP protein were measured. Increased HNE levels on NEP proteins and reduced enzymatic activities of NEP were observed in the HNE- or oligomeric Aβ42-treated cells. Xan reduced HNE levels on NEP proteins and preserved enzymatic activities of NEP in HNE- or oligomeric Aβ42-treated cells. Xan reduced Aβ42 accumulation and protected neurones against oligomeric Aβ42-induced neurotoxicity through preservation of NEP activities. These findings indicate that Xan possesses therapeutic potential for the treatment of neurodegenerative diseases, including AD, and suggest a potential mechanism for the neuroprotective effects of antioxidants for the prevention of AD.

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Neuroprotective effects of Liriope platyphylla extract against H2O2-induced cytotoxicity in the human SH-SY5Y neuroblastoma cells

Integrative Medicine Research ◽

10.1016/j.imr.2015.04.061 ◽

2015 ◽

Vol 4 (1) ◽

pp. 62

Author(s):

Hee Ra Park ◽

Heeeun Lee ◽

Hwayong Park ◽

Jong Wook Jeon ◽

Won-Kyung Cho ◽

...

Keyword(s):

Neuroblastoma Cells ◽

Neuroprotective Effects ◽

Liriope Platyphylla

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Amarogentin from Gentiana rigescens Franch Exhibits Antiaging and Neuroprotective Effects through Antioxidative Stress

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/3184019 ◽

2020 ◽

Vol 2020 ◽

pp. 1-15

Author(s):

Dejene Disasa ◽

Lihong Cheng ◽

Majid Manzoor ◽

Qian Liu ◽

Ying Wang ◽

...

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Antioxidant Activity ◽

Natural Products ◽

Survival Rate ◽

Pc12 Cells ◽

Neuroprotective Effects ◽

Replicative Lifespan ◽

Antioxidative Stress ◽

Gentiana Rigescens

In the present study, the replicative lifespan assay of yeast was used to guide the isolation of antiaging substance from Gentiana rigescens Franch, a traditional Chinese medicine. A compound with antiaging effect was isolated, and the chemical structure of this molecule as amarogentin was identified by spectral analysis and compared with the reported data. It significantly extended the replicative lifespan of K6001 yeast at doses of 1, 3, and 10 μM. Furthermore, amarogentin improved the survival rate of yeast under oxidative stress by increasing the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and these enzymes’ gene expression. In addition, this compound did not extend the replicative lifespan of sod1, sod2, uth1, and skn7 mutants with K6001 background. These results suggested that amarogentin exhibited antiaging effect on yeast via increase of SOD2, CAT, GPx gene expression, enzyme activity, and antioxidative stress. Moreover, we evaluated antioxidant activity of this natural products using PC12 cell system, a useful model for studying the nervous system at the cellular level. Amarogentin significantly improved the survival rate of PC12 cells under H2O2-induced oxidative stress and increased the activities of SOD and SOD2, and gene expression of SOD2, CAT, GPx, Nrf2, and Bcl-x1. Meanwhile, the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) of PC12 cells were significantly reduced after treatment of the amarogentin. These results indicated that antioxidative stress play an important role for antiaging and neuroprotection of amarogentin. Interestingly, amarogentin exhibited neuritogenic activity in PC12 cells. Therefore, the natural products, amarogentin from G. rigescens with antioxidant activity could be a good candidate molecule to develop drug for treating neurodegenerative diseases.

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