Synthesis, characterization, antioxidant activity and neuroprotective effects of selenium polysaccharide from Radix hedysari

2015 ◽  
Vol 125 ◽  
pp. 161-168 ◽  
Author(s):  
Dongfeng Wei ◽  
Tong Chen ◽  
Mingfei Yan ◽  
Wanghong Zhao ◽  
Fei Li ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Neuroprotective Effects

scholarly journals Amarogentin from Gentiana rigescens Franch Exhibits Antiaging and Neuroprotective Effects through Antioxidative Stress

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Dejene Disasa ◽  
Lihong Cheng ◽  
Majid Manzoor ◽  
Qian Liu ◽  
Ying Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Antioxidant Activity ◽  
Natural Products ◽  
Survival Rate ◽  
Pc12 Cells ◽  
Neuroprotective Effects ◽  
Replicative Lifespan ◽  
Antioxidative Stress ◽  
Gentiana Rigescens

In the present study, the replicative lifespan assay of yeast was used to guide the isolation of antiaging substance from Gentiana rigescens Franch, a traditional Chinese medicine. A compound with antiaging effect was isolated, and the chemical structure of this molecule as amarogentin was identified by spectral analysis and compared with the reported data. It significantly extended the replicative lifespan of K6001 yeast at doses of 1, 3, and 10 μM. Furthermore, amarogentin improved the survival rate of yeast under oxidative stress by increasing the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and these enzymes’ gene expression. In addition, this compound did not extend the replicative lifespan of sod1, sod2, uth1, and skn7 mutants with K6001 background. These results suggested that amarogentin exhibited antiaging effect on yeast via increase of SOD2, CAT, GPx gene expression, enzyme activity, and antioxidative stress. Moreover, we evaluated antioxidant activity of this natural products using PC12 cell system, a useful model for studying the nervous system at the cellular level. Amarogentin significantly improved the survival rate of PC12 cells under H2O2-induced oxidative stress and increased the activities of SOD and SOD2, and gene expression of SOD2, CAT, GPx, Nrf2, and Bcl-x1. Meanwhile, the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) of PC12 cells were significantly reduced after treatment of the amarogentin. These results indicated that antioxidative stress play an important role for antiaging and neuroprotection of amarogentin. Interestingly, amarogentin exhibited neuritogenic activity in PC12 cells. Therefore, the natural products, amarogentin from G. rigescens with antioxidant activity could be a good candidate molecule to develop drug for treating neurodegenerative diseases.

scholarly journals Development of Novel 2-Acetylphenol-O-Alkylhydroxyethylamine Derivatives as Multifunctional Agents for Alzheimer’s Disease Treatment

2021 ◽  
Author(s):  
Gaofeng zhu ◽  
xinfeng li ◽  
Jing Yang ◽  
ying he ◽  
jing mi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Metal Ions ◽  
Blood Brain Barrier ◽  
Mixed Type ◽  
Ache Inhibition ◽  
Disease Treatment ◽  
Neuroprotective Effects ◽  
Mao B ◽  
Multifunctional Agents

Abstract Due to the complex pathogenesis of AD, the multi-target-directed ligands (MTDLs) strategy presented the best pharmacological option for AD treatment. Herein, a series of novel 2-acetylphenol-O-alkylhydroxyethylamine derivatives was rationally designed and synthesized. Of these derivatives, 5c was a good multifunctional agent (eeAChE IC50 =7.9 μM, MAO-B IC50 = 9.9 μM, BACE1 IC50 = 8.3 μM) in vitro and displayed a mixed-type AChE inhibition, which could bind to the CAS and PAS of AChE. Compound 5c also exhibited good antioxidant activity (ORAC = 2.5 eq) and neuroprotective effects. Furthermore, compound 5c was a selective metal ions chelator. And it could cross blood-brain barrier in vitro and complied with drug-like properties rule of 5. Therefore, compound 5c was a promising multifunctional agent for the treatment of AD.

Salen‑manganese complexes for controlling ROS damage: Neuroprotective effects, antioxidant activity and kinetic studies

2020 ◽  
Vol 203 ◽  
pp. 110918 ◽  
Author(s):  
Lara Rouco ◽  
Andrea Liberato ◽  
M. Jesús Fernández-Trujillo ◽  
Angeles Máñez ◽  
Manuel G. Basallote ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Kinetic Studies ◽  
Manganese Complexes ◽  
Neuroprotective Effects

scholarly journals Protective effect of medicinal fungus Xylaria nigripes mycelia extracts against hydrogen peroxide-induced apoptosis in PC12 cells

2017 ◽  
Vol 30 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Rupesh D Divate ◽  
Pei-Ming Wang ◽  
Chiun-Chuang Wang ◽  
Su-Tze Chou ◽  
Chen-Tien Chang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Pc12 Cells ◽  
Water Extract ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Hot Water ◽  
Neuroprotective Effects ◽  
Medicinal Fungus ◽  
Induced Apoptosis

Xylaria nigripes ( XN) is a medicinal fungus that was used traditionally as a diuretic, nerve tonic, and for treating insomnia and trauma. In this study, we elucidated possible mechanisms of neuroprotective effects of XN mycelia extracts. XN mycelia were produced by fermentation. Hot water extract and 70% ethanol extract of XN mycelia were evaluated on hydrogen peroxide (H2O2)-induced apoptosis in PC12, a rat pheochromocytoma cell line. Both XN extracts effectively protected PC12 cells against H2O2-induced cell damage by inhibiting release of lactate dehydrogenase, decreasing DNA damage, restoring mitochondrial membrane potential, and arresting abnormal apoptosis through upregulation of Bcl-2 and downregulation of Bax and caspase 3. Compared to water extract, ethanol extract showed not only greater neuroprotective effects but also a higher antioxidant activity by scavenging DPPH radicals, inhibiting lipid peroxidation, and reducing power. High phenolic content and antioxidant activity may provide the neuroprotective properties of XN ethanol extract.

scholarly journals Phytochemical Composition, Antioxidant Activity, and Neuroprotective Effect ofTerminalia chebulaRetzius Extracts

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Chia Lin Chang ◽  
Che San Lin
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Neuroprotective Effect ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Cupric Sulfate ◽  
Neuroprotective Effects

The objectives of this study were to determine phytochemical compositions, chemiluminescence antioxidant activities, and neuroprotective effects on PC12 cells for water, methanol, and 95% ethanol extracts of the air-dried fruit ofTerminalia chebulaRetzius. The water extract afforded the greatest yield, and total phenolic and tannin content. The methanol extract yielded the greatest total triterpenoid content. Based on four chemiluminescence antioxidant assays, the three extracts showed various degrees of antioxidant activity. The methanol extract showed good antioxidant activity based on the horseradish peroxidase-luminol-hydrogen peroxide (H2O2) assay. The water extract appeared to have good antioxidant activities in cupric sulfate-Phen-Vc-H2O2and luminol-H2O2assays. Pyrogallol-luminol assay showed the 95% ethanol extract to have good antioxidant activity. The methanol and water extracts presented neuroprotective activities on H2O2-induced PC12 cell death at 0.5–5.0 μg/mL. Further investigations are necessary to verify these activitiesin vivo.

Sign in / Sign up

Export Citation Format

Share Document