Evaluation of histology and sperm parameters of testes treated by lycopene against cyclophosphamide that induced testicular toxicity in Male rats

The incorporation of cisplatin (CP) as a cytotoxic antineoplastic agent in most chemotherapeutic protocols is a challenge due to its toxic effect on testicular tissues. Natural compounds present a promising trend in research, so a new nutraceutical formulation (NCF) was designed to diminish CP spermatotoxicity. A combination of three nutraceutical materials, 250 mg Spirulina platensis powder (SP), 25 mg Tribulus terrestris L. extract (TT), and 100 mg fish oil (FO) were formulated in self-nanoemulsifying self-nanosuspension (SNESNS). SP was loaded into the optimized self-nanoemulsifying system (30% FO, 50% span 80/cremophor EL and 20% isopropanol) and mixed with TT aqueous solution to form SNESNS. For the SP, phytochemical profiling revealed the presence of valuable amounts of fatty acids (FAs), amino acids, flavonoids, polyphenols, vitamins, and minerals. Transmission electron microscopy (TEM) and particle size analysis confirmed the formation of nanoemulsion-based nanosuspension upon dilution. Method validation of the phytochemical constituents in NCF has been developed. Furthermore, NCF was biologically evaluated on male Wistar rats and revealed the improvement of spermatozoa, histopathological features, and biochemical markers over the CP and each ingredient group. Our findings suggest the potential of NCF with SNESNS as a delivery system against CP-induced testicular toxicity in male rats.

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Chlorella vulgaris ameliorates testicular toxicity induced by deltamethrin in male rats via modulating oxidative stress

Andrologia ◽

10.1111/and.13214 ◽

2018 ◽

Vol 51 (3) ◽

pp. e13214 ◽

Cited By ~ 3

Author(s):

Eman Osama ◽

Azza A. A. Galal ◽

Hany Abdalla ◽

Sawsan M. A. El-Sheikh

Keyword(s):

Oxidative Stress ◽

Chlorella Vulgaris ◽

Male Rats ◽

Testicular Toxicity

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Protective Effects of Wheat Sprout on Testicular Toxicity in Male Rats Exposed to Lead

Reproductive System & Sexual Disorders ◽

10.4172/2161-038x.1000156 ◽

2015 ◽

Vol 04 (04) ◽

Cited By ~ 2

Author(s):

Masoud Adibmoradi ◽

Hassan Morovvati

Keyword(s):

Male Rats ◽

Testicular Toxicity ◽

Protective Effects ◽

Wheat Sprout

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Curcumin Along With Fe3O4 Nanoparticles Improved Sperm Parameters In Rats With Testicular Ischemia

Galen Medical Journal ◽

10.31661/gmj.v10i0.2034 ◽

2021 ◽

Vol 10 ◽

pp. e2034

Author(s):

Shabnam Zarei Moradi ◽

Seyed Abdolhamid Angaji ◽

Mitra Salehi ◽

Mehrdad Hashemi

Keyword(s):

Fe3o4 Nanoparticles ◽

Sperm Concentration ◽

Concomitant Administration ◽

Testicular Tissue ◽

Sperm Parameters ◽

Male Rats ◽

Semen Parameters ◽

Control Group ◽

Fe3o4 Nps ◽

Testicular Ischemia

Background: Ischemic/reperfusion (I/R) in testicular tissue is one reason for the worldwide increase in male infertility. In the present study, we assessed the effects of curcumin and Fe3O4 nanoparticles (NPs) on sperm parameters in rats with I/R damage. Materials and Methods: Forty-eight adult male rats were divided into two groups (n=24 per group): control and torsion/detorsion. The control and torsion/detorsion groups were divided into four subgroups include sham, Fe3O4 NPs, curcumin, and Fe3O4 NPs+curcumin. After the rats were sacrificed, semen was collected from their epididymal tissues to assess sperm viability, motility, concentration, and morphology. Results: Curcumin significantly improved viability, motility, and normal sperm morphology in rats with I/R damage compared to the control group; however, it did not have a significant effect on sperm concentration (P<0.001). Fe3O4 NPs alone decreased all sperm parameters in the control and I/R rats (P<0.001). However, concomitant administration of Fe3O4 nanoparticles with curcumin significantly improved sperm parameters in rats with I/R damage (P<0.001). Conclusion: The increase in all semen parameters in the experimental groups with concomitant use of Fe3O4 NPs plus curcumin indicated that green synthesis of NPs could be recommended for future clinical studies.

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Melatonin and alpha lipoic acid attenuate lopinavir/ritonavir - induced testicular toxicity in albino rats

Macedonian Pharmaceutical Bulletin ◽

10.33320/maced.pharm.bull.2016.62.02.002 ◽

2016 ◽

Vol 62 (2) ◽

pp. 17-24

Author(s):

Elias Adikwu ◽

Brambaifa Nelson ◽

Wolfe Atuboyedia Obianime

Keyword(s):

Lipoic Acid ◽

Serum Testosterone ◽

Sperm Parameters ◽

Oral Exposure ◽

Albino Rats ◽

Sperm Cells ◽

Testicular Toxicity ◽

Alpha Lipoic Acid ◽

Testosterone Levels ◽

Oral Doses

The use of lopinavir/ritonavir (LPV/r) could be associated with testicular toxicity as a limiting factor. The present study evaluated the effects of melatonin (MT) and alpha lipoic (ALA) acid on LPV/r–induced testicular toxicity in male albino rats. Eighty five male albino rats used for this study were randomized into 6 groups (A-F). Rats in groups A1 and A2 served as placebo and solvent control and were orally exposed to water and 1% ethanol, respectively. Rats in group B were exposed to oral doses of MT (10 mg kg-1/day), ALA (10 mg kg-1/day) and combined doses of MT and ALA, respectively. Rats in group C were exposed to oral doses of LPV/r (22.9/5.71 - 91.4/22.9 mg kg-1/ day), respectively. Rats in group D-F were exposed to oral doses of MT (10 mg kg-1/day), ALA (10 mg kg-1/day) and combined doses of MT and ALA prior to oral exposure to LPV/r (22.9/5.71 - 91.4/22.9 mg kg-1/day), respectively. At the end of 60 days of exposure to drugs, rats were sacrificed; blood was collected and serum extracted and evaluated for testosterone. Testes were collected and evaluated for sperm parameters. LPV/r-treated rats showed significant (P<0.05) and dose-dependent decreases in sperm count, sperm motility, sperm viability and serum testosterone levels with increases in abnormal sperm cells, debris, and primordial sperm cells when compared to placebo control. However, LPV/r-induced changes in sperm parameters and serum testosterone levels were attenuated in rats pretreated with MT and ALA. The best effects were observed in rats pretreated with combined doses of MT and ALA. Melatonin and alpha lipoic acid have potential to reduce testicular toxicity associated with lopinavir/ritonavir treatment.

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Effect of atenolol on cadmium-induced testicular toxicity in male rats

Reproductive Toxicology ◽

10.1016/s0890-6238(01)00184-8 ◽

2001 ◽

Vol 15 (6) ◽

pp. 699-704 ◽

Cited By ~ 57

Author(s):

Narendra M. Biswas ◽

Ranojoy Sen Gupta ◽

Alok Chattopadhyay ◽

Gargi Roy Choudhury ◽

Mohitosh Sarkar

Keyword(s):

Male Rats ◽

Testicular Toxicity

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Ethyl acetate fraction of Spathodea campanulata (Bignoniaceae) attenuates lead acetate induced testicular toxicity in male Wistar rats

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2019-0289 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Glory Olubusayo Adesiyan ◽

Temitope Olaoluwa Talabi ◽

Oluwafemi Ezekiel Kale ◽

Temitope Funmi Ogundare

Keyword(s):

Ethyl Acetate ◽

Lead Acetate ◽

Octadecenoic Acid ◽

Morphological Characteristics ◽

Acid Methyl Ester ◽

Ethyl Acetate Fraction ◽

Male Rats ◽

Bark Extract ◽

Seminiferous Tubules ◽

Testicular Toxicity

Abstract Objectives The use of Spathodea campanulata in folklore medicine for the management of reproductive disorders has been poorly reported. We sought to investigate the protective potential of the ethyl acetate fraction of S. campanulata stem bark extract (EFSC) on lead acetate-induced (LA) testicular toxicity in male rats. Methods Animals during a 28 days treatment received dimethyl sulfoxide (DMSO, 0.1%), LA (20 mg/kg), and EFSC (200 mg/kg). Others received EFSC only (100, 200, and 400 mg/kg) or vitamin E (100 mg/kg) 1 h prior to LA (20 mg/kg) administration. Results LA administration decreased sperm counts and motility by 36.39 and 40.69% respectively in rats. Also, LA-untreated rats showed elevated malondialdehyde (MDA) and decreased total proteins in testis (260, 33%) and epididymis (62, 29%) respectively. However, EFSC (100, 200, or 400 mg/kg) administrations improved sperm morphological characteristics as well as antioxidant status in LA-treated rats. EFSC (400 mg/kg) showed improved testis seminiferous tubules that were almost normal in the LA-treated rats. Further, EFSC contains a high 9-octadecenoic acid methyl ester. Conclusions Overall, evidence by LA-induced testicular toxicity, EFSC provides chemopreventive roles via antioxidant mechanisms.

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Silver Nanoparticles Stimulates Spermatogenesis Impairments and Hematological Alterations in Testis and Epididymis of Male Rats

Molecules ◽

10.3390/molecules25051063 ◽

2020 ◽

Vol 25 (5) ◽

pp. 1063 ◽

Cited By ~ 4

Author(s):

Janet Olayemi Olugbodi ◽

Oladipupo David ◽

Ene Naomi Oketa ◽

Bashir Lawal ◽

Bamidele Joseph Okoli ◽

...

Keyword(s):

Silver Nanoparticles ◽

Hematological Parameters ◽

Silver Ions ◽

Thiol Group ◽

Sperm Parameters ◽

Male Rats ◽

Ag Nps ◽

Hematological Indices ◽

Electron Microscopies ◽

Pharmaceutical Application

The potential pharmaceutical application of nanoparticles has led to the toxicity within the male reproductive system. In the present study, the effects of silver nanoparticles (Ag-NPs) on hematological parameters, free radical generation, antioxidant system, sperm parameters, and organ histo-morphometry in male rats were investigated. Ag-NPs were produced by the reduction of silver ions, while the formation of which was monitored by UV–visible spectrophotometry. Zeta potential, transmission, and scanning electron microscopies were applied for the characterization of AgNPs. A total of 30 rats were divided into 6 groups and were sub-dermally exposed to Ag-NPs at the dosage of 0 (control), 10, and 50 mg/kg bodyweight (bw) doses for either 7 or 28 days. Ag-NP administration altered hematological indices and caused dose-dependent decreases in sperm motility, velocity, kinematic parameters, concentrations of luteinizing hormone, follicle-stimulating hormone, and testosterone. In the epididymis and testis, the concentrations of malondialdehyde and peroxide increases while superoxide dismutase, catalase, reduced glutathione, and total thiol group decreases. These findings suggest that Ag-NP triggered hormonal imbalance and induce oxidative stress in testis and epididymis; which negatively affect sperm parameters of male rats.

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